Restriction map for
pFN21AE2544
(Circular) (Six-base) MAPSORT of: pFN21AE2544.seq Check: 7210 from: 1 to: 7936
pFN21AE2544 7936 bp
With 182 enzymes: SgfI *
September 30, 2014 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2154 2963 5041 2154
Size: 809 2078 5049
Fragments arranged by size:
5049 2078 809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5746 278
Size: 53 83 186 936 4210 2468
Fragments arranged by size:
4210 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2372 5279 1937
Size: 435 2907 4594
Fragments arranged by size:
4594 2907 435
Acc65I G'GTAC_C
Cuts at: 2277 5257 2277
Size: 2980 4956
AclI AA'CG_TT
Cuts at: 1563 6063 6436 1563
Size: 4500 373 3063
Fragments arranged by size:
4500 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 2802 2990 3111 3504 3531 5999 7383
Size: 978 192 188 121 393 27 2468 1384
Cuts at: 7383 7651 1632
Size: 268 1917
Fragments arranged by size:
2468 1917 1384 978 393 268 192 188
121 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7739
AflIII A'CryG_T
Cuts at: 1203 6835 7812 1203
Size: 5632 977 1327
Fragments arranged by size:
5632 1327 977
AgeI A'CCGG_T
Cuts at: 6767 6767
Size: 7936
AhdI GACnn_n'nnGTC
Cuts at: 6665 6665
Size: 7936
AleI CACnn'nnGTG
Cuts at: 1166 3176 1166
Size: 2010 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 7904
AlwNI CAG_nnn'CTG
Cuts at: 2846 2978 4085 5679 7251 2846
Size: 132 1107 1594 1572 3531
Fragments arranged by size:
3531 1594 1572 1107 132
ApaLI G'TGCA_C
Cuts at: 5993 7149 5993
Size: 1156 6780
ApoI r'AATT_y
Cuts at: 1493 2630 2902 3023 3432 5245 5471 5623 7732
Size: 1137 272 121 409 1813 226 152 2109
Cuts at: 7732 1493
Size: 1697
Fragments arranged by size:
2109 1813 1697 1137 409 272 226 152
121
AseI AT'TA_AT
Cuts at: 160 1032 6490 160
Size: 872 5458 1606
Fragments arranged by size:
5458 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7936
AvaI C'yCGr_G
Cuts at: 1870 1943 5261 1870
Size: 73 3318 4545
Fragments arranged by size:
4545 3318 73
AvrII C'CTAG_G
Cuts at: 2009 2009
Size: 7936
BamHI G'GATC_C
Cuts at: 2307 3163 4591 5266 5633 2307
Size: 856 1428 675 367 4610
Fragments arranged by size:
4610 1428 856 675 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2277 2909 5257 6712 618
Size: 325 217 627 490 632 2348 1455 1842
Fragments arranged by size:
2348 1842 1455 632 627 490 325 217
BanII G_rGCy'C
Cuts at: 1391 3298 5255 1391
Size: 1907 1957 4072
Fragments arranged by size:
4072 1957 1907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3294 3762 4140 5185 928
Size: 971 1395 468 378 1045 3679
Fragments arranged by size:
3679 1395 1045 971 468 378
BbvCI CC'TCA_GC
Cuts at: 3441 3441
Size: 7936
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3551 3585 6127 6161 1903
Size: 34 50 34 1530 34 2542 34 3678
Fragments arranged by size:
3678 2542 1530 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5828 7038 5828
Size: 1210 6726
BclI T'GATC_A
Cuts at: 1548 4566 1548
Size: 3018 4918
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6547 136
Size: 107 122 71 631 605 4875 1525
Fragments arranged by size:
4875 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4663 7931 4663
Size: 3268 4668
BlpI GC'TnA_GC
Cuts at: 5353 5353
Size: 7936
Bme1580I G_kGCm'C
Cuts at: 1790 5997 7153 1790
Size: 4207 1156 2573
Fragments arranged by size:
4207 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7936
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3268 3784 3838
Size: 416 53 83 399 24 1851 516 54
Cuts at: 3838 5667 6625 442
Size: 1829 958 1753
Fragments arranged by size:
1851 1829 1753 958 516 416 399 83
54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6596 1239
Size: 5357 2579
Bpu10I CC'TnA_GC
Cuts at: 3188 3441 5157 3188
Size: 253 1716 5967
Fragments arranged by size:
5967 1716 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3378 3930 6061 6926 7224 3378
Size: 552 2131 865 298 4090
Fragments arranged by size:
4090 2131 865 552 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 6599 882
Size: 617 909 4191 2219
Fragments arranged by size:
4191 2219 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 5727 493
Size: 711 4523 2702
Fragments arranged by size:
4523 2702 711
BsaBI GATnn'nnATC
Cuts at: 4235 7930 4235
Size: 3695 4241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5743 6125 6760 275
Size: 53 83 186 936 4210 382 635 1451
Fragments arranged by size:
4210 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4227 6369 6767 7041 7188 7599 4227
Size: 2142 398 274 147 411 4564
Fragments arranged by size:
4564 2142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4614 4644 1178
Size: 30 165 30 3211 30 4470
Fragments arranged by size:
4470 3211 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3933 5097 1186
Size: 2747 1164 4025
Fragments arranged by size:
4025 2747 1164
BseYI C'CCAG_C
Cuts at: 2793 3856 4149 7139 7635 2793
Size: 1063 293 2990 496 3094
Fragments arranged by size:
3094 2990 1063 496 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3334 3877 5104 1163
Size: 573 1598 543 1227 3995
Fragments arranged by size:
3995 1598 1227 573 543
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5386 6147 7175 1540
Size: 275 186 3385 761 1028 2301
Fragments arranged by size:
3385 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3298 3410 5997 6082 7153 3298
Size: 112 2587 85 1071 4081
Fragments arranged by size:
4081 2587 1071 112 85
BsmI GAATG_Cn'
Cuts at: 3192 5447 5540 3192
Size: 2255 93 5588
Fragments arranged by size:
5588 2255 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2274 2274
Size: 7936
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3298 3410 5255 5997 6082 7153 1391
Size: 399 1508 112 1845 742 85 1071 2174
Fragments arranged by size:
2174 1845 1508 1071 742 399 112 85
BspEI T'CCGG_A
Cuts at: 7599 7599
Size: 7936
BspHI T'CATG_A
Cuts at: 5825 7555 5825
Size: 1730 6206
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2963 5041 5115 5291 844
Size: 1310 809 2078 74 176 3489
Fragments arranged by size:
3489 2078 1310 809 176 74
BsrBI CCG'CTC
Cuts at: 1394 1823 5823 1394
Size: 429 4000 3507
Fragments arranged by size:
4000 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3887 6431 6605 65
Size: 1183 2639 2544 174 1396
Fragments arranged by size:
2639 2544 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6580 6767 1700
Size: 110 4770 187 2869
Fragments arranged by size:
4770 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3826 96
Size: 3730 4206
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7936
BssSI C'ACGA_G
Cuts at: 3815 5996 7008 3815
Size: 2181 1012 4743
Fragments arranged by size:
4743 2181 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7936
BstBI TT'CG_AA
Cuts at: 3546 6740 3546
Size: 3194 4742
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7936
BstXI CCAn_nnnn'nTGG
Cuts at: 2132 2213 4161 2132
Size: 81 1948 5907
Fragments arranged by size:
5907 1948 81
BstYI r'GATC_y
Cuts at: 1972 2307 3163 4591 4663 5266 5633 6018 6035
Size: 335 856 1428 72 603 367 385 17
Cuts at: 6035 7487 7573 7585 7931 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1428 856 603 385 367 346
335 86 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2913 4586 513
Size: 552 1848 1673 3863
Fragments arranged by size:
3863 1848 1673 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7197
Fragments arranged by size:
7197 683 56
BtsI GCAGTG_nn'
Cuts at: 3760 3958 5452 6246 6266 3760
Size: 198 1494 794 20 5430
Fragments arranged by size:
5430 1494 794 198 20
ClaI AT'CG_AT
Cuts at: 1554 3699 1554
Size: 2145 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7901
DraI TTT'AAA
Cuts at: 5240 5240
Size: 7936
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6943 809
Size: 507 5627 1802
Fragments arranged by size:
5627 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3205 3307 4087
Size: 54 1425 50 275 18 1375 102 780
Cuts at: 4087 5383 6272 7901 8
Size: 1296 889 1629 43
Fragments arranged by size:
1629 1425 1375 1296 889 780 275 102
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5383 1537
Size: 275 3571 4090
Fragments arranged by size:
4090 3571 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 2868 3507 3771 4160 4446 4818 5866
Size: 1233 282 639 264 389 286 372 1048
Cuts at: 5866 1353
Size: 3423
Fragments arranged by size:
3423 1233 1048 639 389 372 286 282
264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6523 6897 7043 387
Size: 1064 138 4934 374 146 1280
Fragments arranged by size:
4934 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3296 3296
Size: 7936
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2610 2802 2990 3111 3504 3531 5999
Size: 393 978 192 188 121 393 27 2468
Cuts at: 5999 6596 7383 7651 1239
Size: 597 787 268 1524
Fragments arranged by size:
2468 1524 978 787 597 393 393 268
192 188 121 27
EcoO109I rG'GnC_Cy
Cuts at: 3262 3262
Size: 7936
EcoRI G'AATT_C
Cuts at: 5245 5623 5245
Size: 378 7558
EcoRV GAT'ATC
Cuts at: 3694 3694
Size: 7936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4157 4157
Size: 7936
FspI TGC'GCA
Cuts at: 6442 6442
Size: 7936
HaeII r_GCGC'y
Cuts at: 1678 7083 1678
Size: 5405 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 2822 2854 3557
Size: 32 135 32 1027 32 191 32 703
Cuts at: 3557 3589 3681 3713 3770 3802 4614 4646 4948
Size: 32 92 32 57 32 812 32 302
Cuts at: 4948 4980 5196 5228 6577 6609 6651 6683 1373
Size: 32 216 32 1349 32 42 32 2626
Fragments arranged by size:
2626 1349 1027 812 703 302 216 191
135 92 57 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2373 3849 5280 5526 669
Size: 988 281 435 1476 1431 246 3079
Fragments arranged by size:
3079 1476 1431 988 435 281 246
HindIII A'AGCT_T
Cuts at: 748 3060 3486 3528 4321 748
Size: 2312 426 42 793 4363
Fragments arranged by size:
4363 2312 793 426 42
HpaI GTT'AAC
Cuts at: 3849 5526 3849
Size: 1677 6259
KpnI G_GTAC'C
Cuts at: 2281 5261 2281
Size: 2980 4956
MfeI C'AATT_G
Cuts at: 2211 5535 2211
Size: 3324 4612
MluI A'CGCG_T
Cuts at: 7812 7812
Size: 7936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3753 7050 7234 1305
Size: 2448 3297 184 2007
Fragments arranged by size:
3297 2448 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3207 3309 4089 10
Size: 54 1425 343 1375 102 780 3857
Fragments arranged by size:
3857 1425 1375 780 343 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3176 3365 4199 5894 6253 6412 518
Size: 648 2010 189 834 1695 359 159 2042
Fragments arranged by size:
2042 2010 1695 834 648 359 189 159
MspA1I CmG'CkG
Cuts at: 1606 2846 3683 4330 5352 6029 7177 7422 1606
Size: 1240 837 647 1022 677 1148 245 2120
Fragments arranged by size:
2120 1240 1148 1022 837 677 647 245
NcoI C'CATG_G
Cuts at: 513 1065 2913 513
Size: 552 1848 5536
Fragments arranged by size:
5536 1848 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7936
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7936
NotI GC'GGCC_GC
Cuts at: 5383 5383
Size: 7936
NspI r_CATG'y
Cuts at: 1132 1229 2947 3055 4776 5294 6839 1132
Size: 97 1718 108 1721 518 1545 2229
Fragments arranged by size:
2229 1721 1718 1545 518 108 97
PasI CC'CwG_GG
Cuts at: 1348 5030 1348
Size: 3682 4254
PciI A'CATG_T
Cuts at: 6835 6835
Size: 7936
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5075 1108
Size: 3967 3969
PfoI T'CCnGG_A
Cuts at: 3639 3987 3639
Size: 348 7588
PmeI GTTT'AAAC
Cuts at: 5240 5240
Size: 7936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6005 6037 7518 7550 6005
Size: 32 1481 32 6391
Fragments arranged by size:
6391 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3262 3262
Size: 7936
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7936
PsiI TTA'TAA
Cuts at: 5506 5506
Size: 7936
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4911 4943 4911
Size: 32 7904
PstI C_TGCA'G
Cuts at: 830 3229 3477 3767 5112 5288 830
Size: 2399 248 290 1345 176 3478
Fragments arranged by size:
3478 2399 1345 290 248 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7936
PvuII CAG'CTG
Cuts at: 2846 3683 4330 2846
Size: 837 647 6452
Fragments arranged by size:
6452 837 647
SacI G_AGCT'C
Cuts at: 3298 3298
Size: 7936
SalI G'TCGA_C
Cuts at: 1936 2371 5278 1936
Size: 435 2907 4594
Fragments arranged by size:
4594 2907 435
SanDI GG'GwC_CC
Cuts at: 3262 3262
Size: 7936
SapI GCTCTTCn'nnn_
Cuts at: 2586 2586
Size: 7936
SbfI CC_TGCA'GG
Cuts at: 5288 5288
Size: 7936
ScaI AGT'ACT
Cuts at: 6184 7896 6184
Size: 1712 6224
SfcI C'TryA_G
Cuts at: 826 1045 3225 3473 3654 3763 3867 4429 5108
Size: 219 2180 248 181 109 104 562 679
Cuts at: 5108 5284 6419 7100 7291 826
Size: 176 1135 681 191 1471
Fragments arranged by size:
2180 1471 1135 681 679 562 248 219
191 181 176 109 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7936
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7936
SmaI CCC'GGG
Cuts at: 1872 5263 1872
Size: 3391 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3393 3945 6040 6941 7203 820
Size: 197 926 1450 552 2095 901 262 1553
Fragments arranged by size:
2095 1553 1450 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 5727 493
Size: 5234 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7936
SphI G_CATG'C
Cuts at: 1132 3055 5294 1132
Size: 1923 2239 3774
Fragments arranged by size:
3774 2239 1923
SspI AAT'ATT
Cuts at: 5 52 3328 5860 5
Size: 47 3276 2532 2081
Fragments arranged by size:
3276 2532 2081 47
StuI AGG'CCT
Cuts at: 1517 2587 1517
Size: 1070 6866
StyI C'CwwG_G
Cuts at: 513 1065 2009 2282 2850 2913 2967 3837 5375
Size: 552 944 273 568 63 54 870 1538
Cuts at: 5375 513
Size: 3074
Fragments arranged by size:
3074 1538 944 870 568 552 273 63
54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2660 3306 5963 5980 6133 6318 6763
Size: 1255 1080 646 2657 17 153 185 445
Cuts at: 6763 325
Size: 1498
Fragments arranged by size:
2657 1498 1255 1080 646 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2533 3255 3826
Size: 274 80 33 51 1443 556 722 571
Cuts at: 3826 6182 7894 96
Size: 2356 1712 138
Fragments arranged by size:
2356 1712 1443 722 571 556 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7896 7928 7896
Size: 32 7904
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7936
XbaI T'CTAG_A
Cuts at: 5272 5272
Size: 7936
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7936
XmaI C'CCGG_G
Cuts at: 1870 5261 1870
Size: 3391 4545
XmnI GAAnn'nnTTC
Cuts at: 3118 3432 6065 3118
Size: 314 2633 4989
Fragments arranged by size:
4989 2633 314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5744 276
Size: 53 83 186 936 4210 2468
Fragments arranged by size:
4210 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI