Restriction map for
pFN21AE2542
(Circular) (Six-base) MAPSORT of: pFN21AE2542.seq Check: 4269 from: 1 to: 7408
pFN21AE2542 7408 bp
With 182 enzymes: SgfI *
January 27, 2015 16:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2854 2854
Size: 7408
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4169 5218 278
Size: 53 83 186 936 2633 1049 2468
Fragments arranged by size:
2633 2468 1049 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 3007 4751 1937
Size: 729 341 1744 4594
Fragments arranged by size:
4594 1744 729 341
Acc65I G'GTAC_C
Cuts at: 4729 4729
Size: 7408
AclI AA'CG_TT
Cuts at: 1563 5535 5908 1563
Size: 3972 373 3063
Fragments arranged by size:
3972 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2864 3837 5471 6855 7123 1632
Size: 1232 973 1634 1384 268 1917
Fragments arranged by size:
1917 1634 1384 1232 973 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7211
AflIII A'CryG_T
Cuts at: 1203 2116 3677 6307 7284 1203
Size: 913 1561 2630 977 1327
Fragments arranged by size:
2630 1561 1327 977 913
AgeI A'CCGG_T
Cuts at: 6239 6239
Size: 7408
AhdI GACnn_n'nnGTC
Cuts at: 6137 6137
Size: 7408
AleI CACnn'nnGTG
Cuts at: 1166 2786 4448 1166
Size: 1620 1662 4126
Fragments arranged by size:
4126 1662 1620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4136 4711 1184
Size: 2952 575 3881
Fragments arranged by size:
3881 2952 575
AlwNI CAG_nnn'CTG
Cuts at: 3380 5151 6723 3380
Size: 1771 1572 4065
Fragments arranged by size:
4065 1771 1572
ApaI G_GGCC'C
Cuts at: 3410 3410
Size: 7408
ApaLI G'TGCA_C
Cuts at: 5465 6621 5465
Size: 1156 6252
ApoI r'AATT_y
Cuts at: 1493 2220 2554 4649 4673 4717 4943 5095 7204
Size: 727 334 2095 24 44 226 152 2109
Cuts at: 7204 1493
Size: 1697
Fragments arranged by size:
2109 2095 1697 727 334 226 152 44
24
AscI GG'CGCG_CC
Cuts at: 2398 2398
Size: 7408
AseI AT'TA_AT
Cuts at: 160 1032 3888 5962 160
Size: 872 2856 2074 1606
Fragments arranged by size:
2856 2074 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7408
AvaI C'yCGr_G
Cuts at: 1870 1943 4733 1870
Size: 73 2790 4545
Fragments arranged by size:
4545 2790 73
BamHI G'GATC_C
Cuts at: 4738 5105 4738
Size: 367 7041
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 4313 4729 6184 618
Size: 325 217 627 1079 1447 416 1455 1842
Fragments arranged by size:
1842 1455 1447 1079 627 416 325 217
BanII G_rGCy'C
Cuts at: 1391 2044 2419 3410 4671 4727 1391
Size: 653 375 991 1261 56 4072
Fragments arranged by size:
4072 1261 991 653 375 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5599 5633 1903
Size: 34 3662 34 3678
Fragments arranged by size:
3678 3662 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5300 6510 5300
Size: 1210 6198
BclI T'GATC_A
Cuts at: 1548 2913 3750 1548
Size: 1365 837 5206
Fragments arranged by size:
5206 1365 837
BfrBI ATG'CAT
Cuts at: 2896 4698 2896
Size: 1802 5606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2025 6019
Size: 107 122 71 631 605 337 16 3994
Cuts at: 6019 136
Size: 1525
Fragments arranged by size:
3994 1525 631 605 337 122 107 71
16
BglII A'GATC_T
Cuts at: 7403 7403
Size: 7408
BlpI GC'TnA_GC
Cuts at: 4825 4825
Size: 7408
Bme1580I G_kGCm'C
Cuts at: 1790 2783 3410 5469 6625 1790
Size: 993 627 2059 1156 2573
Fragments arranged by size:
2573 2059 1156 993 627
BmgBI CAC'GTC
Cuts at: 1318 3712 3905 1318
Size: 2394 193 4821
Fragments arranged by size:
4821 2394 193
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3701 5139 6097
Size: 416 53 83 399 24 2284 1438 958
Cuts at: 6097 442
Size: 1753
Fragments arranged by size:
2284 1753 1438 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2553 2640 2655 3190 3860 4260 6068 1239
Size: 1314 87 15 535 670 400 1808 2579
Fragments arranged by size:
2579 1808 1314 670 535 400 87 15
Bpu10I CC'TnA_GC
Cuts at: 4299 4299
Size: 7408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5533 6398 6696 5533
Size: 865 298 6245
Fragments arranged by size:
6245 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3611 4343 6071 882
Size: 617 2112 732 1728 2219
Fragments arranged by size:
2219 2112 1728 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 5199 493
Size: 711 3995 2702
Fragments arranged by size:
3995 2702 711
BsaBI GATnn'nnATC
Cuts at: 4539 7402 4539
Size: 2863 4545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4166 5215 5597 6232
Size: 53 83 186 936 2633 1049 382 635
Cuts at: 6232 275
Size: 1451
Fragments arranged by size:
2633 1451 1049 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5841 6239 6513 6660 7071 5841
Size: 398 274 147 411 6178
Fragments arranged by size:
6178 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3664 3694 4219 4249 1178
Size: 30 165 30 2261 30 525 30 4337
Fragments arranged by size:
4337 2261 525 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4083 1186
Size: 2897 4511
BseYI C'CCAG_C
Cuts at: 4203 4601 6611 7107 4203
Size: 398 2010 496 4504
Fragments arranged by size:
4504 2010 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4018 1163
Size: 573 2282 4553
Fragments arranged by size:
4553 2282 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4858 5619 6647 1540
Size: 275 186 2857 761 1028 2301
Fragments arranged by size:
2857 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3368 5469 5554 6625 3368
Size: 2101 85 1071 4151
Fragments arranged by size:
4151 2101 1071 85
BsmI GAATG_Cn'
Cuts at: 4919 5012 4919
Size: 93 7315
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2419 2783 3368 3410 4671 4727
Size: 399 254 375 364 585 42 1261 56
Cuts at: 4727 5469 5554 6625 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1261 1071 742 585 399 375 364
254 85 56 42
BspEI T'CCGG_A
Cuts at: 7071 7071
Size: 7408
BspHI T'CATG_A
Cuts at: 5297 7027 5297
Size: 1730 5678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2854 4763 844
Size: 2010 1909 3489
Fragments arranged by size:
3489 2010 1909
BsrBI CCG'CTC
Cuts at: 1394 1823 5295 1394
Size: 429 3472 3507
Fragments arranged by size:
3507 3472 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3205 3481 4118 5903 6077 65
Size: 1183 1957 276 637 1785 174 1396
Fragments arranged by size:
1957 1785 1396 1183 637 276 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 6052 6239 1700
Size: 110 239 4003 187 2869
Fragments arranged by size:
4003 2869 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7408
BssHII G'CGCG_C
Cuts at: 1925 2398 1925
Size: 473 6935
BssSI C'ACGA_G
Cuts at: 5468 6480 5468
Size: 1012 6396
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7408
BstBI TT'CG_AA
Cuts at: 6212 6212
Size: 7408
BstEII G'GTnAC_C
Cuts at: 1184 3556 1184
Size: 2372 5036
BstXI CCAn_nnnn'nTGG
Cuts at: 3552 4360 3552
Size: 808 6600
BstYI r'GATC_y
Cuts at: 1972 4048 4738 5105 5490 5507 6959 7045 7057
Size: 2076 690 367 385 17 1452 86 12
Cuts at: 7057 7403 1972
Size: 346 1977
Fragments arranged by size:
2076 1977 1452 690 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2667 2667
Size: 7408
BtgI C'CryG_G
Cuts at: 513 1065 2004 3814 4317 513
Size: 552 939 1810 503 3604
Fragments arranged by size:
3604 1810 939 552 503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6669
Fragments arranged by size:
6669 683 56
BtsI GCAGTG_nn'
Cuts at: 2681 3520 4627 4924 5718 5738 2681
Size: 839 1107 297 794 20 4351
Fragments arranged by size:
4351 1107 839 794 297 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7408
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7373
DraI TTT'AAA
Cuts at: 3192 3217 4712 3192
Size: 25 1495 5888
Fragments arranged by size:
5888 1495 25
DraIII CAC_nnn'GTG
Cuts at: 4010 4043 4010
Size: 33 7375
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6415 809
Size: 507 5099 1802
Fragments arranged by size:
5099 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4405 4855 5744
Size: 54 1425 50 275 18 2575 450 889
Cuts at: 5744 7373 8
Size: 1629 43
Fragments arranged by size:
2575 1629 1425 889 450 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4855 1537
Size: 275 3043 4090
Fragments arranged by size:
4090 3043 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 4582 4697 5338 1353
Size: 917 2312 115 641 3423
Fragments arranged by size:
3423 2312 917 641 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5995 6369 6515 387
Size: 1064 138 4406 374 146 1280
Fragments arranged by size:
4406 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2553 2640 2655 2864 3190 3837 3860
Size: 393 921 87 15 209 326 647 23
Cuts at: 3860 4260 5471 6068 6855 7123 1239
Size: 400 1211 597 787 268 1524
Fragments arranged by size:
1524 1211 921 787 647 597 400 393
326 268 209 87 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2474 2474
Size: 7408
EcoO109I rG'GnC_Cy
Cuts at: 2359 2453 2528 3406 3407 2359
Size: 94 75 878 1 6360
Fragments arranged by size:
6360 878 94 75 1
EcoRI G'AATT_C
Cuts at: 4717 5095 4717
Size: 378 7030
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3269 4661 3269
Size: 1392 6016
FspI TGC'GCA
Cuts at: 5914 5914
Size: 7408
HaeII r_GCGC'y
Cuts at: 1678 4293 6555 1678
Size: 2615 2262 2531
Fragments arranged by size:
2615 2531 2262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4314 4346 6049 6081 6123
Size: 32 135 32 2742 32 1703 32 42
Cuts at: 6123 6155 1373
Size: 32 2626
Fragments arranged by size:
2742 2626 1703 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2161 2539 4752 4998 669
Size: 988 281 223 378 2213 246 3079
Fragments arranged by size:
3079 2213 988 378 281 246 223
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7408
HpaI GTT'AAC
Cuts at: 2161 4998 2161
Size: 2837 4571
KpnI G_GTAC'C
Cuts at: 4733 4733
Size: 7408
MfeI C'AATT_G
Cuts at: 5007 5007
Size: 7408
MluI A'CGCG_T
Cuts at: 7284 7284
Size: 7408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2325 2924 3569 6522 6706 1305
Size: 1020 599 645 2953 184 2007
Fragments arranged by size:
2953 2007 1020 645 599 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4407 10
Size: 54 1425 343 2575 3011
Fragments arranged by size:
3011 2575 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2202 2786 4358 4448 4548 5366 5725
Size: 648 1036 584 1572 90 100 818 359
Cuts at: 5725 5884 518
Size: 159 2042
Fragments arranged by size:
2042 1572 1036 818 648 584 359 159
100 90
MspA1I CmG'CkG
Cuts at: 1606 2183 4824 5501 6649 6894 1606
Size: 577 2641 677 1148 245 2120
Fragments arranged by size:
2641 2120 1148 677 577 245
NaeI GCC'GGC
Cuts at: 2051 2051
Size: 7408
NcoI C'CATG_G
Cuts at: 513 1065 2004 3814 513
Size: 552 939 1810 4107
Fragments arranged by size:
4107 1810 939 552
NdeI CA'TA_TG
Cuts at: 387 3490 3940 387
Size: 3103 450 3855
Fragments arranged by size:
3855 3103 450
NgoMIV G'CCGG_C
Cuts at: 2049 2049
Size: 7408
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7408
NotI GC'GGCC_GC
Cuts at: 4855 4855
Size: 7408
NruI TCG'CGA
Cuts at: 2072 2072
Size: 7408
NsiI A_TGCA'T
Cuts at: 2898 4700 2898
Size: 1802 5606
NspI r_CATG'y
Cuts at: 1132 1229 2120 3681 3979 4553 4698 4766 6311
Size: 97 891 1561 298 574 145 68 1545
Cuts at: 6311 1132
Size: 2229
Fragments arranged by size:
2229 1561 1545 891 574 298 145 97
68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7408
PciI A'CATG_T
Cuts at: 2116 3677 6307 2116
Size: 1561 2630 3217
Fragments arranged by size:
3217 2630 1561
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 4058 4285 1108
Size: 1116 1834 227 4231
Fragments arranged by size:
4231 1834 1116 227
PfoI T'CCnGG_A
Cuts at: 2030 2530 4237 2030
Size: 500 1707 5201
Fragments arranged by size:
5201 1707 500
PmeI GTTT'AAAC
Cuts at: 4712 4712
Size: 7408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5477 5509 6990 7022 5477
Size: 32 1481 32 5863
Fragments arranged by size:
5863 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2453 2528 2359
Size: 94 75 7239
Fragments arranged by size:
7239 94 75
PshAI GACnn'nnGTC
Cuts at: 1613 3834 1613
Size: 2221 5187
PsiI TTA'TAA
Cuts at: 4978 4978
Size: 7408
PspOMI G'GGCC_C
Cuts at: 3406 3406
Size: 7408
PstI C_TGCA'G
Cuts at: 830 4433 4463 4760 830
Size: 3603 30 297 3478
Fragments arranged by size:
3603 3478 297 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7408
PvuII CAG'CTG
Cuts at: 2183 2183
Size: 7408
SalI G'TCGA_C
Cuts at: 1936 4750 1936
Size: 2814 4594
SbfI CC_TGCA'GG
Cuts at: 4433 4760 4433
Size: 327 7081
ScaI AGT'ACT
Cuts at: 2849 5656 7368 2849
Size: 2807 1712 2889
Fragments arranged by size:
2889 2807 1712
SfcI C'TryA_G
Cuts at: 826 1045 2604 2904 3008 4429 4459 4608 4756
Size: 219 1559 300 104 1421 30 149 148
Cuts at: 4756 5891 6572 6763 826
Size: 1135 681 191 1471
Fragments arranged by size:
1559 1471 1421 1135 681 300 219 191
149 148 104 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7408
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7408
SmaI CCC'GGG
Cuts at: 1872 4735 1872
Size: 2863 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5512 6413 6675 820
Size: 197 926 3569 901 262 1553
Fragments arranged by size:
3569 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5199 493
Size: 4706 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7408
SphI G_CATG'C
Cuts at: 1132 4553 4698 4766 1132
Size: 3421 145 68 3774
Fragments arranged by size:
3774 3421 145 68
SspI AAT'ATT
Cuts at: 5 52 2082 2331 3643 4104 5332 5
Size: 47 2030 249 1312 461 1228 2081
Fragments arranged by size:
2081 2030 1312 1228 461 249 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7408
StyI C'CwwG_G
Cuts at: 513 1065 2004 2448 3664 3814 4052 4847 513
Size: 552 939 444 1216 150 238 795 3074
Fragments arranged by size:
3074 1216 939 795 552 444 238 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 5435 5452 5605 5790 6235 325
Size: 1255 1313 2542 17 153 185 445 1498
Fragments arranged by size:
2542 1498 1313 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2594 2847 2977
Size: 274 80 33 51 1443 617 253 130
Cuts at: 2977 3859 4072 5654 7366 96
Size: 882 213 1582 1712 138
Fragments arranged by size:
1712 1582 1443 882 617 274 253 213
138 130 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4048 4080 4219 4251 7368 7400 4048
Size: 32 139 32 3117 32 4056
Fragments arranged by size:
4056 3117 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4562 1316
Size: 3246 4162
XbaI T'CTAG_A
Cuts at: 4744 4744
Size: 7408
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7408
XmaI C'CCGG_G
Cuts at: 1870 4733 1870
Size: 2863 4545
XmnI GAAnn'nnTTC
Cuts at: 2388 3249 4268 5537 2388
Size: 861 1019 1269 4259
Fragments arranged by size:
4259 1269 1019 861
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4167 5216 276
Size: 53 83 186 936 2633 1049 2468
Fragments arranged by size:
2633 2468 1049 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BbvCI BplI BsiWI
BsmBI Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI