Restriction map for
pFN21AE2540
(Circular) (Six-base) MAPSORT of: pFN21AE2540.seq Check: 5825 from: 1 to: 7627
pFN21AE2540 7627 bp
With 182 enzymes: SgfI *
September 19, 2014 18:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5437 278
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3000 4970 1937
Size: 1063 1970 4594
Fragments arranged by size:
4594 1970 1063
Acc65I G'GTAC_C
Cuts at: 4948 4948
Size: 7627
AclI AA'CG_TT
Cuts at: 1563 5754 6127 1563
Size: 4191 373 3063
Fragments arranged by size:
4191 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2223 2306 2429 3327 3879 4633 4691 4760
Size: 591 83 123 898 552 754 58 69
Cuts at: 4760 5690 7074 7342 1632
Size: 930 1384 268 1917
Fragments arranged by size:
1917 1384 930 898 754 591 552 268
123 83 69 58
AfeI AGC'GCT
Cuts at: 2153 2153
Size: 7627
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7430
AflIII A'CryG_T
Cuts at: 1203 2440 6526 7503 1203
Size: 1237 4086 977 1327
Fragments arranged by size:
4086 1327 1237 977
AgeI A'CCGG_T
Cuts at: 2659 6458 2659
Size: 3799 3828
AhdI GACnn_n'nnGTC
Cuts at: 6356 6356
Size: 7627
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7627
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7627
AlwNI CAG_nnn'CTG
Cuts at: 4046 4124 4738 5370 6942 4046
Size: 78 614 632 1572 4731
Fragments arranged by size:
4731 1572 632 614 78
ApaI G_GGCC'C
Cuts at: 4150 4150
Size: 7627
ApaLI G'TGCA_C
Cuts at: 5684 6840 5684
Size: 1156 6471
ApoI r'AATT_y
Cuts at: 1493 2598 2649 4490 4936 5162 5314 7423 1493
Size: 1105 51 1841 446 226 152 2109 1697
Fragments arranged by size:
2109 1841 1697 1105 446 226 152 51
AseI AT'TA_AT
Cuts at: 160 1032 6181 160
Size: 872 5149 1606
Fragments arranged by size:
5149 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
AvaI C'yCGr_G
Cuts at: 1870 1943 2589 4952 1870
Size: 73 646 2363 4545
Fragments arranged by size:
4545 2363 646 73
BamHI G'GATC_C
Cuts at: 3802 4957 5324 3802
Size: 1155 367 6105
Fragments arranged by size:
6105 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3011 3499 4766 4948 6403
Size: 325 217 627 1224 488 1267 182 1455
Cuts at: 6403 618
Size: 1842
Fragments arranged by size:
1842 1455 1267 1224 627 488 325 217
182
BanII G_rGCy'C
Cuts at: 1391 2025 4150 4385 4647 4946 1391
Size: 634 2125 235 262 299 4072
Fragments arranged by size:
4072 2125 634 299 262 235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 3050 3136 928
Size: 971 333 818 86 5419
Fragments arranged by size:
5419 971 818 333 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5818 5852 1903
Size: 34 3881 34 3678
Fragments arranged by size:
3881 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5519 6729 5519
Size: 1210 6417
BclI T'GATC_A
Cuts at: 1548 2367 1548
Size: 819 6808
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3309 3314 6238
Size: 107 122 71 631 605 1637 5 2924
Cuts at: 6238 136
Size: 1525
Fragments arranged by size:
2924 1637 1525 631 605 122 107 71
5
BglII A'GATC_T
Cuts at: 7622 7622
Size: 7627
BlpI GC'TnA_GC
Cuts at: 2839 5044 2839
Size: 2205 5422
Bme1580I G_kGCm'C
Cuts at: 1790 2091 3014 3502 3612 3624 4150 5688 6844
Size: 301 923 488 110 12 526 1538 1156
Cuts at: 6844 1790
Size: 2573
Fragments arranged by size:
2573 1538 1156 923 526 488 301 110
12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7627
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 2967 4667
Size: 416 53 83 399 24 661 889 1700
Cuts at: 4667 5358 6316 442
Size: 691 958 1753
Fragments arranged by size:
1753 1700 958 889 691 661 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2990 3624 3717 4074 4297 4471 6287 1239
Size: 1751 634 93 357 223 174 1816 2579
Fragments arranged by size:
2579 1816 1751 634 357 223 174 93
Bpu10I CC'TnA_GC
Cuts at: 4515 4641 4515
Size: 126 7501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 5752 6617 6915 2198
Size: 3554 865 298 2910
Fragments arranged by size:
3554 2910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2778 4449 6290 882
Size: 617 1279 1671 1841 2219
Fragments arranged by size:
2219 1841 1671 1279 617
BsaAI yAC'GTr
Cuts at: 493 1204 5418 493
Size: 711 4214 2702
Fragments arranged by size:
4214 2702 711
BsaBI GATnn'nnATC
Cuts at: 4734 7621 4734
Size: 2887 4740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5434 5816 6451 275
Size: 53 83 186 936 3901 382 635 1451
Fragments arranged by size:
3901 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2489 2659 6060 6458 6732 6879 7290 2489
Size: 170 3401 398 274 147 411 2826
Fragments arranged by size:
3401 2826 411 398 274 170 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 3765 3795 1178
Size: 30 165 30 636 30 1696 30 5010
Fragments arranged by size:
5010 1696 636 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 3236 1186
Size: 976 1074 5577
Fragments arranged by size:
5577 1074 976
BseYI C'CCAG_C
Cuts at: 2831 3292 3323 3482 4128 4747 4923 6830 7326
Size: 461 31 159 646 619 176 1907 496
Cuts at: 7326 2831
Size: 3132
Fragments arranged by size:
3132 1907 646 619 496 461 176 159
31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2111 1163
Size: 573 375 6679
Fragments arranged by size:
6679 573 375
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5077 5838 6866 1540
Size: 275 186 3076 761 1028 2301
Fragments arranged by size:
3076 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2864 2953 4422 4647 5688 5773 6844 2864
Size: 89 1469 225 1041 85 1071 3647
Fragments arranged by size:
3647 1469 1071 1041 225 89 85
BsmI GAATG_Cn'
Cuts at: 3436 3925 5138 5231 3436
Size: 489 1213 93 5832
Fragments arranged by size:
5832 1213 489 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2091 2864 2953 3014 3502 3612
Size: 399 235 66 773 89 61 488 110
Cuts at: 3612 3624 4150 4385 4422 4647 4946 5688 5773
Size: 12 526 235 37 225 299 742 85
Cuts at: 5773 6844 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 773 742 526 488 399 299
235 235 225 110 89 85 66 61
37 12
BspEI T'CCGG_A
Cuts at: 7290 7290
Size: 7627
BspHI T'CATG_A
Cuts at: 5516 7246 5516
Size: 1730 5897
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4982 844
Size: 4138 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5514 1394
Size: 429 3691 3507
Fragments arranged by size:
3691 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3114 4603 4902 6122 6296 65
Size: 1183 1866 1489 299 1220 174 1396
Fragments arranged by size:
1866 1489 1396 1220 1183 299 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2659 3308 6271 6458 1700
Size: 110 849 649 2963 187 2869
Fragments arranged by size:
2963 2869 849 649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7627
BssHII G'CGCG_C
Cuts at: 1925 4102 1925
Size: 2177 5450
BssSI C'ACGA_G
Cuts at: 5687 6699 5687
Size: 1012 6615
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4124 794
Size: 3330 4297
BstBI TT'CG_AA
Cuts at: 6431 6431
Size: 7627
BstEII G'GTnAC_C
Cuts at: 1184 3268 1184
Size: 2084 5543
BstXI CCAn_nnnn'nTGG
Cuts at: 3821 3821
Size: 7627
BstYI r'GATC_y
Cuts at: 1972 2311 2955 3103 3802 4735 4957 5324 5709
Size: 339 644 148 699 933 222 367 385
Cuts at: 5709 5726 7178 7264 7276 7622 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 933 699 644 385 367 346
339 222 148 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4160 513
Size: 552 939 2156 3980
Fragments arranged by size:
3980 2156 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6888
Fragments arranged by size:
6888 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 5143 5937 5957 3268
Size: 1875 794 20 4938
Fragments arranged by size:
4938 1875 794 20
ClaI AT'CG_AT
Cuts at: 1554 4701 1554
Size: 3147 4480
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7592
DraI TTT'AAA
Cuts at: 2728 3714 4931 2728
Size: 986 1217 5424
Fragments arranged by size:
5424 1217 986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 3823 4479 6634 809
Size: 507 792 1715 656 2155 1802
Fragments arranged by size:
2155 1802 1715 792 656 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2273 4918
Size: 54 1425 50 275 18 177 266 2645
Cuts at: 4918 5074 5963 7592 8
Size: 156 889 1629 43
Fragments arranged by size:
2645 1629 1425 889 275 266 177 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5074 1537
Size: 275 3262 4090
Fragments arranged by size:
4090 3262 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4652 5557 1353
Size: 3299 905 3423
Fragments arranged by size:
3423 3299 905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6214 6588 6734 387
Size: 1064 138 4625 374 146 1280
Fragments arranged by size:
4625 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4645 4645
Size: 7627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 2306 2429 2990 3327 3624 3717
Size: 393 591 83 123 561 337 297 93
Cuts at: 3717 3879 4074 4297 4471 4633 4691 4760 5690
Size: 162 195 223 174 162 58 69 930
Cuts at: 5690 6287 7074 7342 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 591 561 393 337
297 268 223 195 174 162 162 123
93 83 69 58
EcoO109I rG'GnC_Cy
Cuts at: 3380 4146 3380
Size: 766 6861
EcoRI G'AATT_C
Cuts at: 2598 2649 4936 5314 2598
Size: 51 2287 378 4911
Fragments arranged by size:
4911 2287 378 51
EcoRV GAT'ATC
Cuts at: 3334 3334
Size: 7627
FspI TGC'GCA
Cuts at: 3120 6133 3120
Size: 3013 4614
HaeII r_GCGC'y
Cuts at: 1678 2155 6774 1678
Size: 477 4619 2531
Fragments arranged by size:
4619 2531 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2484 2516 3778
Size: 32 135 32 467 32 413 32 1262
Cuts at: 3778 3810 3821 3853 3931 3963 4370 4402 6268
Size: 32 11 32 78 32 407 32 1866
Cuts at: 6268 6300 6342 6374 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1866 1262 467 413 407 135 78
42 32 32 32 32 32 32 32
32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4177 4971 5217 669
Size: 988 281 2239 794 246 3079
Fragments arranged by size:
3079 2239 988 794 281 246
HindIII A'AGCT_T
Cuts at: 748 4820 748
Size: 4072 3555
HpaI GTT'AAC
Cuts at: 5217 5217
Size: 7627
KpnI G_GTAC'C
Cuts at: 4952 4952
Size: 7627
MfeI C'AATT_G
Cuts at: 2640 5226 2640
Size: 2586 5041
MluI A'CGCG_T
Cuts at: 7503 7503
Size: 7627
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 2082 2541 4431 6741 6925 1305
Size: 720 57 459 1890 2310 184 2007
Fragments arranged by size:
2310 2007 1890 720 459 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2275 10
Size: 54 1425 343 443 5362
Fragments arranged by size:
5362 1425 443 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2439 5585 5944 6103 518
Size: 648 1273 3146 359 159 2042
Fragments arranged by size:
3146 2042 1273 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4124 4310 4923 5043 5720 6868 7113 1606
Size: 2518 186 613 120 677 1148 245 2120
Fragments arranged by size:
2518 2120 1148 677 613 245 186 120
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 7627
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6136
Fragments arranged by size:
6136 939 552
NdeI CA'TA_TG
Cuts at: 387 4171 387
Size: 3784 3843
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 7627
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7627
NotI GC'GGCC_GC
Cuts at: 5074 5074
Size: 7627
NspI r_CATG'y
Cuts at: 1132 1229 2444 3321 3725 3895 4183 4985 6530
Size: 97 1215 877 404 170 288 802 1545
Cuts at: 6530 1132
Size: 2229
Fragments arranged by size:
2229 1545 1215 877 802 404 288 170
97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 6961
PciI A'CATG_T
Cuts at: 2440 6526 2440
Size: 4086 3541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2645 3473 3495 1108
Size: 1537 828 22 5240
Fragments arranged by size:
5240 1537 828 22
PfoI T'CCnGG_A
Cuts at: 4854 4854
Size: 7627
PmeI GTTT'AAAC
Cuts at: 4931 4931
Size: 7627
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2935 2967 4533 4565 5696 5728 7209 7241 2935
Size: 32 1566 32 1131 32 1481 32 3321
Fragments arranged by size:
3321 1566 1481 1131 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3380 3380
Size: 7627
PshAI GACnn'nnGTC
Cuts at: 1613 4253 1613
Size: 2640 4987
PsiI TTA'TAA
Cuts at: 5197 5197
Size: 7627
PspOMI G'GGCC_C
Cuts at: 4146 4146
Size: 7627
PstI C_TGCA'G
Cuts at: 830 4111 4979 830
Size: 3281 868 3478
Fragments arranged by size:
3478 3281 868
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7627
PvuII CAG'CTG
Cuts at: 4124 4310 4124
Size: 186 7441
SacI G_AGCT'C
Cuts at: 4647 4647
Size: 7627
SalI G'TCGA_C
Cuts at: 1936 4969 1936
Size: 3033 4594
SanDI GG'GwC_CC
Cuts at: 3380 3380
Size: 7627
SapI GCTCTTCn'nnn_
Cuts at: 4652 4652
Size: 7627
SbfI CC_TGCA'GG
Cuts at: 4979 4979
Size: 7627
ScaI AGT'ACT
Cuts at: 5875 7587 5875
Size: 1712 5915
SexAI A'CCwGG_T
Cuts at: 2547 2547
Size: 7627
SfcI C'TryA_G
Cuts at: 826 1045 2556 4107 4975 6110 6791 6982 826
Size: 219 1511 1551 868 1135 681 191 1471
Fragments arranged by size:
1551 1511 1471 1135 868 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7627
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7627
SmaI CCC'GGG
Cuts at: 1872 4954 1872
Size: 3082 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 5731 6632 6894 820
Size: 197 926 234 3554 901 262 1553
Fragments arranged by size:
3554 1553 926 901 262 234 197
SnaBI TAC'GTA
Cuts at: 493 5418 493
Size: 4925 2702
SpeI A'CTAG_T
Cuts at: 152 2786 152
Size: 2634 4993
SphI G_CATG'C
Cuts at: 1132 3895 4985 1132
Size: 2763 1090 3774
Fragments arranged by size:
3774 2763 1090
SspI AAT'ATT
Cuts at: 5 52 2467 2713 3592 5551 5
Size: 47 2415 246 879 1959 2081
Fragments arranged by size:
2415 2081 1959 879 246 47
StuI AGG'CCT
Cuts at: 1517 3351 1517
Size: 1834 5793
StyI C'CwwG_G
Cuts at: 513 1065 2004 2358 3352 3954 5066 513
Size: 552 939 354 994 602 1112 3074
Fragments arranged by size:
3074 1112 994 939 602 552 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3496 3701 4473 5654 5671 5824 6009
Size: 1255 1916 205 772 1181 17 153 185
Cuts at: 6009 6454 325
Size: 445 1498
Fragments arranged by size:
1916 1498 1255 1181 772 445 205 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5873 7585 96
Size: 274 80 33 51 1443 3896 1712 138
Fragments arranged by size:
3896 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3765 3797 3910 3942 7587 7619 3765
Size: 32 113 32 3645 32 3773
Fragments arranged by size:
3773 3645 113 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2108 3369 1316
Size: 792 1261 5574
Fragments arranged by size:
5574 1261 792
XbaI T'CTAG_A
Cuts at: 4963 4963
Size: 7627
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7627
XmaI C'CCGG_G
Cuts at: 1870 4952 1870
Size: 3082 4545
XmnI GAAnn'nnTTC
Cuts at: 3904 5756 3904
Size: 1852 5775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5435 276
Size: 53 83 186 936 3901 2468
Fragments arranged by size:
3901 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI
XcmI