Restriction map for
pFN21AE2539
(Circular) (Six-base) MAPSORT of: pFN21AE2539.seq Check: 8951 from: 1 to: 6745
pFN21AE2539 6745 bp
With 182 enzymes: SgfI *
September 19, 2014 18:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4555 278
Size: 53 83 186 936 3019 2468
Fragments arranged by size:
3019 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3777 4088 1937
Size: 1840 311 4594
Fragments arranged by size:
4594 1840 311
Acc65I G'GTAC_C
Cuts at: 4066 4066
Size: 6745
AclI AA'CG_TT
Cuts at: 1563 3986 4872 5245 1563
Size: 2423 886 373 3063
Fragments arranged by size:
3063 2423 886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 2950 3908 4808 6192 6460 1632
Size: 430 888 958 900 1384 268 1917
Fragments arranged by size:
1917 1384 958 900 888 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6548
AflIII A'CryG_T
Cuts at: 1203 2463 5644 6621 1203
Size: 1260 3181 977 1327
Fragments arranged by size:
3181 1327 1260 977
AgeI A'CCGG_T
Cuts at: 5576 5576
Size: 6745
AhdI GACnn_n'nnGTC
Cuts at: 3550 5474 3550
Size: 1924 4821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6745
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6745
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2677 2709 2677
Size: 32 6713
AlwNI CAG_nnn'CTG
Cuts at: 4488 6060 4488
Size: 1572 5173
ApaI G_GGCC'C
Cuts at: 2646 2646
Size: 6745
ApaLI G'TGCA_C
Cuts at: 2984 4802 5958 2984
Size: 1818 1156 3771
Fragments arranged by size:
3771 1818 1156
ApoI r'AATT_y
Cuts at: 1493 4017 4054 4280 4432 6541 1493
Size: 2524 37 226 152 2109 1697
Fragments arranged by size:
2524 2109 1697 226 152 37
AseI AT'TA_AT
Cuts at: 160 1032 5299 160
Size: 872 4267 1606
Fragments arranged by size:
4267 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
AvaI C'yCGr_G
Cuts at: 1870 1943 2680 3485 4070 1870
Size: 73 737 805 585 4545
Fragments arranged by size:
4545 805 737 585 73
BamHI G'GATC_C
Cuts at: 4075 4442 4075
Size: 367 6378
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4066 5521 618
Size: 325 217 627 2279 1455 1842
Fragments arranged by size:
2279 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2646 3475 3845 4064 1391
Size: 1255 829 370 219 4072
Fragments arranged by size:
4072 1255 829 370 219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2887 4011 928
Size: 971 988 1124 3662
Fragments arranged by size:
3662 1124 988 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2324 2358 2720 2754 4936 4970 1903
Size: 34 387 34 362 34 2182 34 3678
Fragments arranged by size:
3678 2182 387 362 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2086 3014 4637 5847 2086
Size: 928 1623 1210 2984
Fragments arranged by size:
2984 1623 1210 928
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6745
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2650 5356 136
Size: 107 122 71 631 605 978 2706 1525
Fragments arranged by size:
2706 1525 978 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3825 6740 3825
Size: 2915 3830
BlpI GC'TnA_GC
Cuts at: 4162 4162
Size: 6745
Bme1580I G_kGCm'C
Cuts at: 1790 2646 2988 3972 4806 5962 1790
Size: 856 342 984 834 1156 2573
Fragments arranged by size:
2573 1156 984 856 834 342
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6745
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3197 4476 5434
Size: 416 53 83 399 24 1780 1279 958
Cuts at: 5434 442
Size: 1753
Fragments arranged by size:
1780 1753 1279 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6745
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3457 3482 3061
Size: 396 25 6324
Fragments arranged by size:
6324 396 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2398 2888 3738 5405 1239
Size: 1159 490 850 1667 2579
Fragments arranged by size:
2579 1667 1159 850 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2657 4870 5735 6033 2648
Size: 9 2213 865 298 3360
Fragments arranged by size:
3360 2213 865 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2767 3133 3973 5408 882
Size: 617 1268 366 840 1435 2219
Fragments arranged by size:
2219 1435 1268 840 617 366
BsaAI yAC'GTr
Cuts at: 493 1204 2466 2617 4536 493
Size: 711 1262 151 1919 2702
Fragments arranged by size:
2702 1919 1262 711 151
BsaBI GATnn'nnATC
Cuts at: 6739 6739
Size: 6745
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4552 4934 5569 275
Size: 53 83 186 936 3019 382 635 1451
Fragments arranged by size:
3019 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2126 5178 5576 5850 5997 6408 2126
Size: 3052 398 274 147 411 2463
Fragments arranged by size:
3052 2463 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2541 2571 1178
Size: 30 165 30 1138 30 5352
Fragments arranged by size:
5352 1138 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2565 3037 3539 1186
Size: 1379 472 502 4392
Fragments arranged by size:
4392 1379 502 472
BseYI C'CCAG_C
Cuts at: 2013 2275 2589 2741 5948 6444 2013
Size: 262 314 152 3207 496 2314
Fragments arranged by size:
3207 2314 496 314 262 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3385 3907 1163
Size: 573 1649 522 4001
Fragments arranged by size:
4001 1649 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4195 4956 5984 1540
Size: 275 186 2194 761 1028 2301
Fragments arranged by size:
2301 2194 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2197 2988 4806 4891 5962 2197
Size: 791 1818 85 1071 2980
Fragments arranged by size:
2980 1818 1071 791 85
BsmI GAATG_Cn'
Cuts at: 4256 4349 4256
Size: 93 6652
BsmBI CGTCTCn'nnnn_
Cuts at: 2547 2547
Size: 6745
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 2646 2988 3475 3845 3972 4064
Size: 399 407 449 342 487 370 127 92
Cuts at: 4064 4806 4891 5962 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 449 407 399 370
342 127 92 85
BspEI T'CCGG_A
Cuts at: 2126 6408 2126
Size: 4282 2463
BspHI T'CATG_A
Cuts at: 4634 6364 4634
Size: 1730 5015
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4100 844
Size: 3256 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4632 1394
Size: 429 2809 3507
Fragments arranged by size:
3507 2809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5240 5414 65
Size: 1183 3992 174 1396
Fragments arranged by size:
3992 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2499 2524 2977 5389 5576 1700
Size: 110 689 25 453 2412 187 2869
Fragments arranged by size:
2869 2412 689 453 187 110 25
BsrGI T'GTAC_A
Cuts at: 96 3936 96
Size: 3840 2905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6745
BssSI C'ACGA_G
Cuts at: 4805 5817 4805
Size: 1012 5733
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3967 794
Size: 3173 3572
BstBI TT'CG_AA
Cuts at: 5549 5549
Size: 6745
BstEII G'GTnAC_C
Cuts at: 1184 2041 3274 1184
Size: 857 1233 4655
Fragments arranged by size:
4655 1233 857
BstXI CCAn_nnnn'nTGG
Cuts at: 3580 3744 3580
Size: 164 6581
BstYI r'GATC_y
Cuts at: 1972 3825 4075 4442 4827 4844 6296 6382 6394
Size: 1853 250 367 385 17 1452 86 12
Cuts at: 6394 6740 1972
Size: 346 1977
Fragments arranged by size:
1977 1853 1452 385 367 346 250 86
17 12
BstZ17I GTA'TAC
Cuts at: 3778 3778
Size: 6745
Bsu36I CC'TnA_GG
Cuts at: 3522 3522
Size: 6745
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 3279 3864 513
Size: 552 939 42 1233 585 3394
Fragments arranged by size:
3394 1233 939 585 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2327 487
Size: 683 56 1101 4905
Fragments arranged by size:
4905 1101 683 56
BtsI GCAGTG_nn'
Cuts at: 3246 3972 4261 5055 5075 3246
Size: 726 289 794 20 4916
Fragments arranged by size:
4916 794 726 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6745
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6710
DraI TTT'AAA
Cuts at: 4049 4049
Size: 6745
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5752 809
Size: 507 4436 1802
Fragments arranged by size:
4436 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 3282 3570
Size: 54 1425 50 275 18 420 1032 288
Cuts at: 3570 4192 5081 6710 8
Size: 622 889 1629 43
Fragments arranged by size:
1629 1425 1032 889 622 420 288 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4192 1537
Size: 275 2380 4090
Fragments arranged by size:
4090 2380 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2827 3100 4675 1353
Size: 1474 273 1575 3423
Fragments arranged by size:
3423 1575 1474 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2750 3248 5332 5706 5852 387
Size: 1064 138 1161 498 2084 374 146 1280
Fragments arranged by size:
2084 1280 1161 1064 498 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2398 2888 2950 3738 3908 4808
Size: 393 430 336 490 62 788 170 900
Cuts at: 4808 5405 6192 6460 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 900 788 787 597 490 430 393
336 268 170 62
EcoO109I rG'GnC_Cy
Cuts at: 3151 3151
Size: 6745
EcoRI G'AATT_C
Cuts at: 4017 4054 4432 4017
Size: 37 378 6330
Fragments arranged by size:
6330 378 37
EcoRV GAT'ATC
Cuts at: 3691 3691
Size: 6745
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 6745
FspI TGC'GCA
Cuts at: 5251 5251
Size: 6745
HaeII r_GCGC'y
Cuts at: 1678 2828 5892 1678
Size: 1150 3064 2531
Fragments arranged by size:
3064 2531 1150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 3320 3352 3536
Size: 32 135 32 533 32 1183 32 184
Cuts at: 3536 3568 3785 3817 5386 5418 5460 5492 1373
Size: 32 217 32 1569 32 42 32 2626
Fragments arranged by size:
2626 1569 1183 533 217 184 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4089 4335 669
Size: 988 281 2151 246 3079
Fragments arranged by size:
3079 2151 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6745
HpaI GTT'AAC
Cuts at: 4335 4335
Size: 6745
KpnI G_GTAC'C
Cuts at: 4070 4070
Size: 6745
MfeI C'AATT_G
Cuts at: 4344 4344
Size: 6745
MluI A'CGCG_T
Cuts at: 6621 6621
Size: 6745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2527 3084 5859 6043 1305
Size: 1222 557 2775 184 2007
Fragments arranged by size:
2775 2007 1222 557 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3572 10
Size: 54 1425 343 1740 3183
Fragments arranged by size:
3183 1740 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 3113 3789 3944 4703 5062 5221
Size: 648 1309 638 676 155 759 359 159
Cuts at: 5221 518
Size: 2042
Fragments arranged by size:
2042 1309 759 676 648 638 359 159
155
MspA1I CmG'CkG
Cuts at: 1606 2013 3070 3568 4161 4838 5986 6231 1606
Size: 407 1057 498 593 677 1148 245 2120
Fragments arranged by size:
2120 1148 1057 677 593 498 407 245
NaeI GCC'GGC
Cuts at: 2526 2979 2526
Size: 453 6292
NcoI C'CATG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 5212
Fragments arranged by size:
5212 939 552 42
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6745
NgoMIV G'CCGG_C
Cuts at: 2524 2977 2524
Size: 453 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6745
NotI GC'GGCC_GC
Cuts at: 4192 4192
Size: 6745
NspI r_CATG'y
Cuts at: 1132 1229 3965 4103 5648 1132
Size: 97 2736 138 1545 2229
Fragments arranged by size:
2736 2229 1545 138 97
PasI CC'CwG_GG
Cuts at: 1348 3199 3854 1348
Size: 1851 655 4239
Fragments arranged by size:
4239 1851 655
PciI A'CATG_T
Cuts at: 5644 5644
Size: 6745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2307 1108
Size: 1199 5546
PmeI GTTT'AAAC
Cuts at: 4049 4049
Size: 6745
PmlI CAC'GTG
Cuts at: 2617 2617
Size: 6745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4814 4846 6327 6359 4814
Size: 32 1481 32 5200
Fragments arranged by size:
5200 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3151 3151
Size: 6745
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6745
PsiI TTA'TAA
Cuts at: 4315 4315
Size: 6745
PspOMI G'GGCC_C
Cuts at: 2642 2642
Size: 6745
PstI C_TGCA'G
Cuts at: 830 4097 830
Size: 3267 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6745
PvuII CAG'CTG
Cuts at: 2013 3568 2013
Size: 1555 5190
SalI G'TCGA_C
Cuts at: 1936 4087 1936
Size: 2151 4594
SbfI CC_TGCA'GG
Cuts at: 4097 4097
Size: 6745
ScaI AGT'ACT
Cuts at: 3497 3587 4993 6705 3497
Size: 90 1406 1712 3537
Fragments arranged by size:
3537 1712 1406 90
SfcI C'TryA_G
Cuts at: 826 1045 4093 5228 5909 6100 826
Size: 219 3048 1135 681 191 1471
Fragments arranged by size:
3048 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6745
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6745
SmaI CCC'GGG
Cuts at: 1872 3487 4072 1872
Size: 1615 585 4545
Fragments arranged by size:
4545 1615 585
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2663 2672 4849 5750 6012 820
Size: 197 926 720 9 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 720 262 197 9
SnaBI TAC'GTA
Cuts at: 493 4536 493
Size: 4043 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6745
SphI G_CATG'C
Cuts at: 1132 3965 4103 1132
Size: 2833 138 3774
Fragments arranged by size:
3774 2833 138
SspI AAT'ATT
Cuts at: 5 52 4669 5
Size: 47 4617 2081
Fragments arranged by size:
4617 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6745
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 3264 4184 513
Size: 552 939 42 1218 920 3074
Fragments arranged by size:
3074 1218 939 920 552 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2744 4772 4789 4942 5127 5572 325
Size: 1255 1164 2028 17 153 185 445 1498
Fragments arranged by size:
2028 1498 1255 1164 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3495 3585 3936
Size: 274 80 33 51 1443 1518 90 351
Cuts at: 3936 4991 6703 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1518 1443 1055 351 274 138 90
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6705 6737 6705
Size: 32 6713
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6745
XbaI T'CTAG_A
Cuts at: 4081 4081
Size: 6745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2304 3067 3634 2304
Size: 763 567 5415
Fragments arranged by size:
5415 763 567
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6745
XmaI C'CCGG_G
Cuts at: 1870 3485 4070 1870
Size: 1615 585 4545
Fragments arranged by size:
4545 1615 585
XmnI GAAnn'nnTTC
Cuts at: 4874 4874
Size: 6745
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4553 276
Size: 53 83 186 936 3019 2468
Fragments arranged by size:
3019 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI DraIII EcoICRI EcoNI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI