Restriction map for
pFN21AE2538
(Circular) (Six-base) MAPSORT of: pFN21AE2538.seq Check: 9722 from: 1 to: 12397
pFN21AE2538 12397 bp
With 182 enzymes: SgfI *
February 10, 2017 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 8167 2599
Size: 5568 6829
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10207 278
Size: 53 83 186 936 8671 2468
Fragments arranged by size:
8671 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2334 2553 5099 5689 5938 9740 1937
Size: 397 219 2546 590 249 3802 4594
Fragments arranged by size:
4594 3802 2546 590 397 249 219
Acc65I G'GTAC_C
Cuts at: 9718 9718
Size: 12397
AclI AA'CG_TT
Cuts at: 1563 6992 10524 10897 1563
Size: 5429 3532 373 3063
Fragments arranged by size:
5429 3532 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2218 2312 2967 3552 4431 4446 4637
Size: 462 124 94 655 585 879 15 191
Cuts at: 4637 6068 6251 6349 6851 6960 7325 7376 8128
Size: 1431 183 98 502 109 365 51 752
Cuts at: 8128 8972 9531 9587 10460 11844 12112 1632
Size: 844 559 56 873 1384 268 1917
Fragments arranged by size:
1917 1431 1384 879 873 844 752 655
585 559 502 462 365 268 191 183
124 109 98 94 56 51 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12200
AflIII A'CryG_T
Cuts at: 1203 2800 4969 5737 5891 8686 8906 11296 12273
Size: 1597 2169 768 154 2795 220 2390 977
Cuts at: 12273 1203
Size: 1327
Fragments arranged by size:
2795 2390 2169 1597 1327 977 768 220
154
AgeI A'CCGG_T
Cuts at: 11228 11228
Size: 12397
AhdI GACnn_n'nnGTC
Cuts at: 6097 11126 6097
Size: 5029 7368
AleI CACnn'nnGTG
Cuts at: 1166 6894 1166
Size: 5728 6669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4078 1184
Size: 2894 9503
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3603 3635 3603
Size: 32 12365
AlwNI CAG_nnn'CTG
Cuts at: 2972 4046 4592 7607 7871 8434 8699 9460 10140
Size: 1074 546 3015 264 563 265 761 680
Cuts at: 10140 11712 2972
Size: 1572 3657
Fragments arranged by size:
3657 3015 1572 1074 761 680 563 546
265 264
ApaI G_GGCC'C
Cuts at: 6345 6345
Size: 12397
ApaLI G'TGCA_C
Cuts at: 10454 11610 10454
Size: 1156 11241
ApoI r'AATT_y
Cuts at: 1493 2427 2877 3023 3707 3715 3920 4522 4541
Size: 934 450 146 684 8 205 602 19
Cuts at: 4541 4903 5020 5227 5351 5430 5557 6068 8076
Size: 362 117 207 124 79 127 511 2008
Cuts at: 8076 9706 9932 10084 12193 1493
Size: 1630 226 152 2109 1697
Fragments arranged by size:
2109 2008 1697 1630 934 684 602 511
450 362 226 207 205 152 146 127
124 117 79 19 8
AseI AT'TA_AT
Cuts at: 160 1032 3813 7926 10951 160
Size: 872 2781 4113 3025 1606
Fragments arranged by size:
4113 3025 2781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12397
AvaI C'yCGr_G
Cuts at: 1870 1943 6777 9198 9722 1870
Size: 73 4834 2421 524 4545
Fragments arranged by size:
4834 4545 2421 524 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4335 4368 5843 5876 4335
Size: 33 1475 33 10856
Fragments arranged by size:
10856 1475 33 33
BamHI G'GATC_C
Cuts at: 5923 6041 8536 9256 9727 10094 5923
Size: 118 2495 720 471 367 8226
Fragments arranged by size:
8226 2495 720 471 367 118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 8680 9718 11173 618
Size: 325 217 627 390 6503 1038 1455 1842
Fragments arranged by size:
6503 1842 1455 1038 627 390 325 217
BanII G_rGCy'C
Cuts at: 1391 3068 3317 5769 6345 6535 7890 9716 1391
Size: 1677 249 2452 576 190 1355 1826 4072
Fragments arranged by size:
4072 2452 1826 1677 1355 576 249 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3410 4652 4936 5040 6566 8174 8978
Size: 971 1511 1242 284 104 1526 1608 804
Cuts at: 8978 9197 9519 928
Size: 219 322 3806
Fragments arranged by size:
3806 1608 1526 1511 1242 971 804 322
284 219 104
BbvCI CC'TCA_GC
Cuts at: 9087 9087
Size: 12397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10588 10622 1903
Size: 34 8651 34 3678
Fragments arranged by size:
8651 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10289 11499 10289
Size: 1210 11187
BclI T'GATC_A
Cuts at: 1548 3985 7732 8614 9234 1548
Size: 2437 3747 882 620 4711
Fragments arranged by size:
4711 3747 2437 882 620
BfrBI ATG'CAT
Cuts at: 4146 5057 4146
Size: 911 11486
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11008 136
Size: 107 122 71 631 605 9336 1525
Fragments arranged by size:
9336 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2711 3215 12392 2711
Size: 504 9177 2716
Fragments arranged by size:
9177 2716 504
BlpI GC'TnA_GC
Cuts at: 3067 7211 7595 8622 9814 3067
Size: 4144 384 1027 1192 5650
Fragments arranged by size:
5650 4144 1192 1027 384
Bme1580I G_kGCm'C
Cuts at: 1790 6321 6345 10458 11614 1790
Size: 4531 24 4113 1156 2573
Fragments arranged by size:
4531 4113 2573 1156 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12397
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3623 5702 5929
Size: 416 53 83 399 24 2206 2079 227
Cuts at: 5929 5961 8585 8713 8789 9436 10128 11086 442
Size: 32 2624 128 76 647 692 958 1753
Fragments arranged by size:
2624 2206 2079 1753 958 692 647 416
399 227 128 83 76 53 32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3234 3256 6770 7207 3234
Size: 22 3514 437 8424
Fragments arranged by size:
8424 3514 437 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2298 3831 4704 5186 8743 9376 9614 11057
Size: 1059 1533 873 482 3557 633 238 1443
Cuts at: 11057 1239
Size: 2579
Fragments arranged by size:
3557 2579 1533 1443 1059 873 633 482
238
Bpu10I CC'TnA_GC
Cuts at: 9087 9087
Size: 12397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4875 5778 5958 6519 6715 8862 9317 10522
Size: 1478 903 180 561 196 2147 455 1205
Cuts at: 10522 11387 11685 3397
Size: 865 298 4109
Fragments arranged by size:
4109 2147 1478 1205 903 865 561 455
298 196 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3441 3491 3849 7193 7491 8187 11060
Size: 617 1942 50 358 3344 298 696 2873
Cuts at: 11060 882
Size: 2219
Fragments arranged by size:
3344 2873 2219 1942 696 617 358 298
50
BsaAI yAC'GTr
Cuts at: 493 1204 2803 5814 5894 10188 493
Size: 711 1599 3011 80 4294 2702
Fragments arranged by size:
4294 3011 2702 1599 711 80
BsaBI GATnn'nnATC
Cuts at: 3187 4614 7731 12391 3187
Size: 1427 3117 4660 3193
Fragments arranged by size:
4660 3193 3117 1427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 10204 10586 11221 275
Size: 53 83 186 936 8671 382 635 1451
Fragments arranged by size:
8671 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5425 9471 10830 11228 11502 11649 12060 5425
Size: 4046 1359 398 274 147 411 5762
Fragments arranged by size:
5762 4046 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4621 4651 4956 4986 7603
Size: 30 165 30 3218 30 305 30 2617
Cuts at: 7603 7633 7773 7803 7949 7979 8741 8771 9024
Size: 30 140 30 146 30 762 30 253
Cuts at: 9024 9054 9554 9584 1178
Size: 30 500 30 3991
Fragments arranged by size:
3991 3218 2617 762 500 305 253 165
146 140 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4971 6032 7089 7970 9141 1186
Size: 3785 1061 1057 881 1171 4442
Fragments arranged by size:
4442 3785 1171 1061 1057 881
BseYI C'CCAG_C
Cuts at: 3839 6687 8540 8579 8976 11600 12096 3839
Size: 2848 1853 39 397 2624 496 4140
Fragments arranged by size:
4140 2848 2624 1853 496 397 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2259 8853 1163
Size: 573 393 130 6594 4707
Fragments arranged by size:
6594 4707 573 393 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7372 9847 10608 11636 1540
Size: 275 186 5371 2475 761 1028 2301
Fragments arranged by size:
5371 2475 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 3068 3317 3370 5769 6535 7212 7558 7601
Size: 1004 249 53 2399 766 677 346 43
Cuts at: 7601 8568 10458 10543 11614 2064
Size: 967 1890 85 1071 2847
Fragments arranged by size:
2847 2399 1890 1071 1004 967 766 677
346 249 85 53 43
BsmI GAATG_Cn'
Cuts at: 6498 7814 9908 10001 6498
Size: 1316 2094 93 8894
Fragments arranged by size:
8894 2094 1316 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5204 5204
Size: 12397
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 3068 3317 3370 5769 6321 6345
Size: 399 274 1004 249 53 2399 552 24
Cuts at: 6345 6535 7212 7558 7601 7890 8568 9716 10458
Size: 190 677 346 43 289 678 1148 742
Cuts at: 10458 10543 11614 1391
Size: 85 1071 2174
Fragments arranged by size:
2399 2174 1148 1071 1004 742 678 677
552 399 346 289 274 249 190 85
53 43 24
BspEI T'CCGG_A
Cuts at: 5425 12060 5425
Size: 6635 5762
BspHI T'CATG_A
Cuts at: 5107 6579 6882 10286 12016 5107
Size: 1472 303 3404 1730 5488
Fragments arranged by size:
5488 3404 1730 1472 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 4217 8167 9752 844
Size: 1755 1618 3950 1585 3489
Fragments arranged by size:
3950 3489 1755 1618 1585
BsrBI CCG'CTC
Cuts at: 1394 1823 8272 10284 1394
Size: 429 6449 2012 3507
Fragments arranged by size:
6449 3507 2012 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2290 5917 9607 10892 11066 65
Size: 1183 1042 3627 3690 1285 174 1396
Fragments arranged by size:
3690 3627 1396 1285 1183 1042 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8782 11041 11228 1700
Size: 110 6972 2259 187 2869
Fragments arranged by size:
6972 2869 2259 187 110
BsrGI T'GTAC_A
Cuts at: 96 3728 6506 8848 8962 96
Size: 3632 2778 2342 114 3531
Fragments arranged by size:
3632 3531 2778 2342 114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12397
BssSI C'ACGA_G
Cuts at: 3369 10457 11469 3369
Size: 7088 1012 4297
Fragments arranged by size:
7088 4297 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 8425 794
Size: 7631 4766
BstBI TT'CG_AA
Cuts at: 6719 11201 6719
Size: 4482 7915
BstEII G'GTnAC_C
Cuts at: 1184 5549 7064 8501 1184
Size: 4365 1515 1437 5080
Fragments arranged by size:
5080 4365 1515 1437
BstXI CCAn_nnnn'nTGG
Cuts at: 6381 6967 7016 8041 8738 6381
Size: 586 49 1025 697 10040
Fragments arranged by size:
10040 1025 697 586 49
BstYI r'GATC_y
Cuts at: 1972 2711 3088 3215 4028 5923 6041 7344 7726
Size: 739 377 127 813 1895 118 1303 382
Cuts at: 7726 8536 9133 9202 9256 9727 10094 10479 10496
Size: 810 597 69 54 471 367 385 17
Cuts at: 10496 11948 12034 12046 12392 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1895 1452 1303 813 810 739 597
471 385 382 377 367 346 127 118
86 69 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 3452 9585 3452
Size: 6133 6264
BtgI C'CryG_G
Cuts at: 513 1065 5028 5241 513
Size: 552 3963 213 7669
Fragments arranged by size:
7669 3963 552 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6394 7533 487
Size: 683 56 5168 1139 5351
Fragments arranged by size:
5351 5168 1139 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 2468 2535 4740 6503 8450 9063 9913 10707
Size: 197 67 2205 1763 1947 613 850 794
Cuts at: 10707 10727 2271
Size: 20 3941
Fragments arranged by size:
3941 2205 1947 1763 850 794 613 197
67 20
ClaI AT'CG_AT
Cuts at: 1554 3862 1554
Size: 2308 10089
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12362
DraI TTT'AAA
Cuts at: 3021 3058 3078 3712 3885 4767 6772 9520 9701
Size: 37 20 634 173 882 2005 2748 181
Cuts at: 9701 3021
Size: 5717
Fragments arranged by size:
5717 2748 2005 882 634 181 173 37
20
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8907 11404 809
Size: 507 7591 2497 1802
Fragments arranged by size:
7591 2497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3347 7369 8028
Size: 54 1425 50 275 18 1517 4022 659
Cuts at: 8028 9844 10733 12362 8
Size: 1816 889 1629 43
Fragments arranged by size:
4022 1816 1629 1517 1425 889 659 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7369 9844 1537
Size: 275 5557 2475 4090
Fragments arranged by size:
5557 4090 2475 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 3387 3442 3517 4656 4918 5131 5598
Size: 978 1056 55 75 1139 262 213 467
Cuts at: 5598 5757 6009 7392 7755 7947 8263 10327 1353
Size: 159 252 1383 363 192 316 2064 3423
Fragments arranged by size:
3423 2064 1383 1139 1056 978 467 363
316 262 252 213 192 159 75 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2562 3223 10984 11358 11504 387
Size: 1064 138 973 661 7761 374 146 1280
Fragments arranged by size:
7761 1280 1064 973 661 374 146 138
EcoICRI GAG'CTC
Cuts at: 3066 3315 5767 6533 3066
Size: 249 2452 766 8930
Fragments arranged by size:
8930 2452 766 249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2218 2298 2312 2967 3552 3831
Size: 393 462 124 80 14 655 585 279
Cuts at: 3831 4431 4446 4637 4704 5186 6068 6251 6349
Size: 600 15 191 67 482 882 183 98
Cuts at: 6349 6851 6960 7325 7376 8128 8743 8972 9376
Size: 502 109 365 51 752 615 229 404
Cuts at: 9376 9531 9587 9614 10460 11057 11844 12112 1239
Size: 155 56 27 846 597 787 268 1524
Fragments arranged by size:
1524 882 846 787 752 655 615 600
597 585 502 482 462 404 393 365
279 268 229 191 183 155 124 109
98 80 67 56 51 27 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3262 4818 8063 3262
Size: 1556 3245 7596
Fragments arranged by size:
7596 3245 1556
EcoO109I rG'GnC_Cy
Cuts at: 6209 6342 6370 9589 6209
Size: 133 28 3219 9017
Fragments arranged by size:
9017 3219 133 28
EcoRI G'AATT_C
Cuts at: 4903 5227 8076 9706 10084 4903
Size: 324 2849 1630 378 7216
Fragments arranged by size:
7216 2849 1630 378 324
EcoRV GAT'ATC
Cuts at: 6196 6196
Size: 12397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6581 7745 6581
Size: 1164 11233
FspI TGC'GCA
Cuts at: 10903 10903
Size: 12397
HaeII r_GCGC'y
Cuts at: 1678 11544 1678
Size: 9866 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2765 2797 2822
Size: 32 135 32 788 32 373 32 25
Cuts at: 2822 2854 3953 3985 4700 4732 4923 4955 5519
Size: 32 1099 32 715 32 191 32 564
Cuts at: 5519 5551 5915 5947 6083 6115 7077 7109 7532
Size: 32 364 32 136 32 962 32 423
Cuts at: 7532 7564 7949 7981 9109 9141 11038 11070 11112
Size: 32 385 32 1128 32 1897 32 42
Cuts at: 11112 11144 1373
Size: 32 2626
Fragments arranged by size:
2626 1897 1128 1099 962 788 715 564
423 385 373 364 191 136 135 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 6990 9741 9987 669
Size: 988 281 5052 2751 246 3079
Fragments arranged by size:
5052 3079 2751 988 281 246
HindIII A'AGCT_T
Cuts at: 748 7388 748
Size: 6640 5757
HpaI GTT'AAC
Cuts at: 6990 9987 6990
Size: 2997 9400
KpnI G_GTAC'C
Cuts at: 9722 9722
Size: 12397
MfeI C'AATT_G
Cuts at: 7054 8958 9996 7054
Size: 1904 1038 9455
Fragments arranged by size:
9455 1904 1038
MluI A'CGCG_T
Cuts at: 12273 12273
Size: 12397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3646 7428 8030 8936 9353 11511 11695 1305
Size: 2341 3782 602 906 417 2158 184 2007
Fragments arranged by size:
3782 2341 2158 2007 906 602 417 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 8030 10
Size: 54 1425 343 6198 4377
Fragments arranged by size:
6198 4377 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3285 4289 4605 5062 5340 5526 5742
Size: 648 2119 1004 316 457 278 186 216
Cuts at: 5742 6379 6654 6894 10355 10714 10873 518
Size: 637 275 240 3461 359 159 2042
Fragments arranged by size:
3461 2119 2042 1004 648 637 457 359
316 278 275 240 216 186 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2318 6894 7801 9813 10490 11638 11883
Size: 408 304 4576 907 2012 677 1148 245
Cuts at: 11883 1606
Size: 2120
Fragments arranged by size:
4576 2120 2012 1148 907 677 408 304
245
NaeI GCC'GGC
Cuts at: 8784 8784
Size: 12397
NcoI C'CATG_G
Cuts at: 513 1065 5028 5241 513
Size: 552 3963 213 7669
Fragments arranged by size:
7669 3963 552 213
NdeI CA'TA_TG
Cuts at: 387 9334 387
Size: 8947 3450
NgoMIV G'CCGG_C
Cuts at: 8782 8782
Size: 12397
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12397
NotI GC'GGCC_GC
Cuts at: 9844 9844
Size: 12397
NsiI A_TGCA'T
Cuts at: 4148 5059 4148
Size: 911 11486
NspI r_CATG'y
Cuts at: 1132 1229 4146 4150 4216 4551 4775 4973 5741
Size: 97 2917 4 66 335 224 198 768
Cuts at: 5741 6552 6653 8690 8910 9649 9755 11300 1132
Size: 811 101 2037 220 739 106 1545 2229
Fragments arranged by size:
2917 2229 2037 1545 811 768 739 335
224 220 198 106 101 97 66 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 12397
PciI A'CATG_T
Cuts at: 4969 5737 8686 8906 11296 4969
Size: 768 2949 220 2390 6070
Fragments arranged by size:
6070 2949 2390 768 220
PflMI CCAn_nnn'nTGG
Cuts at: 1108 8725 1108
Size: 7617 4780
PfoI T'CCnGG_A
Cuts at: 5944 8040 5944
Size: 2096 10301
PmeI GTTT'AAAC
Cuts at: 9701 9701
Size: 12397
PmlI CAC'GTG
Cuts at: 5894 5894
Size: 12397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7274 7306 10466 10498 11979 12011 7274
Size: 32 3160 32 1481 32 7660
Fragments arranged by size:
7660 3160 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 6370 9589 6370
Size: 3219 9178
PshAI GACnn'nnGTC
Cuts at: 1613 3628 7502 1613
Size: 2015 3874 6508
Fragments arranged by size:
6508 3874 2015
PsiI TTA'TAA
Cuts at: 2735 3669 3926 7406 7655 8475 9967 2735
Size: 934 257 3480 249 820 1492 5165
Fragments arranged by size:
5165 3480 1492 934 820 257 249
PspOMI G'GGCC_C
Cuts at: 6341 6341
Size: 12397
PspXI vC'TCGA_Gb
Cuts at: 6777 6777
Size: 12397
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4567 4599 4738 4770 4567
Size: 32 139 32 12194
Fragments arranged by size:
12194 139 32 32
PstI C_TGCA'G
Cuts at: 830 2019 2386 9749 830
Size: 1189 367 7363 3478
Fragments arranged by size:
7363 3478 1189 367
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12397
PvuII CAG'CTG
Cuts at: 2014 2318 6894 7801 2014
Size: 304 4576 907 6610
Fragments arranged by size:
6610 4576 907 304
SacI G_AGCT'C
Cuts at: 3068 3317 5769 6535 3068
Size: 249 2452 766 8930
Fragments arranged by size:
8930 2452 766 249
SalI G'TCGA_C
Cuts at: 1936 9739 1936
Size: 7803 4594
SanDI GG'GwC_CC
Cuts at: 6370 6370
Size: 12397
SapI GCTCTTCn'nnn_
Cuts at: 5131 5598 5757 5131
Size: 467 159 11771
Fragments arranged by size:
11771 467 159
SbfI CC_TGCA'GG
Cuts at: 9749 9749
Size: 12397
ScaI AGT'ACT
Cuts at: 7464 8861 9572 10645 12357 7464
Size: 1397 711 1073 1712 7504
Fragments arranged by size:
7504 1712 1397 1073 711
SexAI A'CCwGG_T
Cuts at: 7203 8509 7203
Size: 1306 11091
SfcI C'TryA_G
Cuts at: 826 1045 2015 2382 2550 5100 7752 8355 8643
Size: 219 970 367 168 2550 2652 603 288
Cuts at: 8643 9745 10880 11561 11752 826
Size: 1102 1135 681 191 1471
Fragments arranged by size:
2652 2550 1471 1135 1102 970 681 603
367 288 219 191 168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12397
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12397
SmaI CCC'GGG
Cuts at: 1872 9724 1872
Size: 7852 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3412 4890 5793 5973 6534 6694
Size: 197 926 1469 1478 903 180 561 160
Cuts at: 6694 6777 8877 9198 9296 10501 11402 11664 820
Size: 83 2100 321 98 1205 901 262 1553
Fragments arranged by size:
2100 1553 1478 1469 1205 926 903 901
561 321 262 197 180 160 98 83
SnaBI TAC'GTA
Cuts at: 493 10188 493
Size: 9695 2702
SpeI A'CTAG_T
Cuts at: 152 4580 152
Size: 4428 7969
SphI G_CATG'C
Cuts at: 1132 4146 4150 4216 4775 6653 9755 1132
Size: 3014 4 66 559 1878 3102 3774
Fragments arranged by size:
3774 3102 3014 1878 559 66 4
SspI AAT'ATT
Cuts at: 5 52 2814 3134 4174 4438 6740 10321 5
Size: 47 2762 320 1040 264 2302 3581 2081
Fragments arranged by size:
3581 2762 2302 2081 1040 320 264 47
StuI AGG'CCT
Cuts at: 1517 8528 9356 1517
Size: 7011 828 4558
Fragments arranged by size:
7011 4558 828
StyI C'CwwG_G
Cuts at: 513 1065 5028 5241 8628 9624 9836 513
Size: 552 3963 213 3387 996 212 3074
Fragments arranged by size:
3963 3387 3074 996 552 213 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5568 8593 10424 10441 10594 10779 11224
Size: 1255 3988 3025 1831 17 153 185 445
Cuts at: 11224 325
Size: 1498
Fragments arranged by size:
3988 3025 1831 1498 1255 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 4388 5513
Size: 274 80 33 51 1443 1751 660 1125
Cuts at: 5513 6506 7462 8746 8848 8859 8962 9570 9630
Size: 993 956 1284 102 11 103 608 60
Cuts at: 9630 9642 10643 12355 96
Size: 12 1001 1712 138
Fragments arranged by size:
1751 1712 1443 1284 1125 1001 993 956
660 608 274 138 103 102 80 60
51 33 12 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4662 4694 4956 4988 6376 6408 7280 7312 7564
Size: 32 262 32 1388 32 872 32 252
Cuts at: 7564 7596 7603 7635 8579 8611 8741 8773 9007
Size: 32 7 32 944 32 130 32 234
Cuts at: 9007 9039 9581 9613 12357 12389 4662
Size: 32 542 32 2744 32 4670
Fragments arranged by size:
4670 2744 1388 944 872 542 262 252
234 130 32 32 32 32 32 32
32 32 32 32 32 7
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 12397
XbaI T'CTAG_A
Cuts at: 3242 9733 3242
Size: 6491 5906
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2470 5649 8038 8581 2470
Size: 3179 2389 543 6286
Fragments arranged by size:
6286 3179 2389 543
XhoI C'TCGA_G
Cuts at: 1943 6777 9198 1943
Size: 4834 2421 5142
Fragments arranged by size:
5142 4834 2421
XmaI C'CCGG_G
Cuts at: 1870 9722 1870
Size: 7852 4545
XmnI GAAnn'nnTTC
Cuts at: 2877 7037 10526 2877
Size: 4160 3489 4748
Fragments arranged by size:
4748 4160 3489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10205 276
Size: 53 83 186 936 8671 2468
Fragments arranged by size:
8671 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BsiWI BstZ17I DraIII FseI
FspAI KasI NarI NruI PacI RsrII SacII SfiI
SfoI SrfI SwaI