Restriction map for
pFN21AE2537
(Circular) (Six-base) MAPSORT of: pFN21AE2537.seq Check: 1266 from: 1 to: 5560
pFN21AE2537 5560 bp
With 182 enzymes: SgfI *
July 25, 2014 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2176 2831 2176
Size: 655 4905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2124 3370 278
Size: 53 83 186 936 588 1246 2468
Fragments arranged by size:
2468 1246 936 588 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2853 2903 1937
Size: 916 50 4594
Fragments arranged by size:
4594 916 50
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 2780 3623 5007 5275 1632
Size: 692 456 843 1384 268 1917
Fragments arranged by size:
1917 1384 843 692 456 268
AflII C'TTAA_G
Cuts at: 820 1017 2597 820
Size: 197 1580 3783
Fragments arranged by size:
3783 1580 197
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 3303 4875 3303
Size: 1572 3988
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2414 2869 3095 3247 5356 1493
Size: 921 455 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 455 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2282 2885 1870
Size: 73 126 213 603 4545
Fragments arranged by size:
4545 603 213 126 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2287 2881 4336 618
Size: 325 217 627 500 594 1455 1842
Fragments arranged by size:
1842 1455 627 594 500 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2879 1391
Size: 625 863 4072
Fragments arranged by size:
4072 863 625
BbeI G_GCGC'C
Cuts at: 2291 2291
Size: 5560
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2221 928
Size: 971 322 4267
Fragments arranged by size:
4267 971 322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2739 3452 4662 2739
Size: 713 1210 3637
Fragments arranged by size:
3637 1210 713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2700 3621 4777 1790
Size: 910 921 1156 2573
Fragments arranged by size:
2573 1156 921 910
BmgBI CAC'GTC
Cuts at: 1318 2242 2701 1318
Size: 924 459 4177
Fragments arranged by size:
4177 924 459
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2155 3351 493
Size: 711 951 1196 2702
Fragments arranged by size:
2702 1196 951 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2288 3367 3749
Size: 53 83 186 936 588 167 1079 382
Cuts at: 3749 4384 275
Size: 635 1451
Fragments arranged by size:
1451 1079 936 635 588 382 186 167
83 53
BsaWI w'CCGG_w
Cuts at: 2218 3993 4391 4665 4812 5223 2218
Size: 1775 398 274 147 411 2555
Fragments arranged by size:
2555 1775 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2273 1186
Size: 940 147 4473
Fragments arranged by size:
4473 940 147
BseYI C'CCAG_C
Cuts at: 2164 2176 2546 4763 5259 2164
Size: 12 370 2217 496 2465
Fragments arranged by size:
2465 2217 496 370 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2568 2693 1163
Size: 573 832 125 4030
Fragments arranged by size:
4030 832 573 125
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2205 3010 3771 4799 1540
Size: 275 186 37 167 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186 167 37
BsiHKAI G_wGCw'C
Cuts at: 3621 3706 4777 3621
Size: 85 1071 4404
Fragments arranged by size:
4404 1071 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2255
Size: 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2700 2879 3621 3706 4777 1391
Size: 399 226 684 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 684 399 226 179 85
BspEI T'CCGG_A
Cuts at: 2218 5223 2218
Size: 3005 2555
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2176 2831 2915 844
Size: 1332 655 84 3489
Fragments arranged by size:
3489 1332 655 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2200 4204 4391 1700
Size: 110 390 2004 187 2869
Fragments arranged by size:
2869 2004 390 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2704 794
Size: 1910 3650
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555
Size: 918 367 385 17 1452 86 12 346
Cuts at: 5555 1972
Size: 1977
Fragments arranged by size:
1977 1452 918 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2247 513
Size: 552 939 48 195 3826
Fragments arranged by size:
3826 939 552 195 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2053 2053
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2489 4567 809
Size: 507 1173 2078 1802
Fragments arranged by size:
2078 1802 1173 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2017 2035
Size: 54 1425 50 275 18 177 10 18
Cuts at: 2035 2080 2202 2244 3007 3896 5525 8
Size: 45 122 42 763 889 1629 43
Fragments arranged by size:
1629 1425 889 763 275 177 122 54
50 45 43 42 18 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 2035 2202 3007 1537
Size: 275 223 167 805 4090
Fragments arranged by size:
4090 805 275 223 167
EarI CTCTTCn'nnn_
Cuts at: 1353 2522 2718 3490 1353
Size: 1169 196 772 3423
Fragments arranged by size:
3423 1169 772 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2403 4147 4521 4667 387
Size: 1064 138 814 1744 374 146 1280
Fragments arranged by size:
1744 1280 1064 814 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2780 3623 4220 5007 5275 1239
Size: 393 692 456 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 692 597 456 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2593 2593
Size: 5560
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 2066 2103 2291 4707 1678
Size: 388 37 188 2416 2531
Fragments arranged by size:
2531 2416 388 188 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373
Size: 32 135 32 2629 32 42 32 2626
Fragments arranged by size:
2629 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 2904 3150 669
Size: 988 281 373 593 246 3079
Fragments arranged by size:
3079 988 593 373 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KasI G'GCGC_C
Cuts at: 2287 2287
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 2565 3159 2565
Size: 594 4966
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4674 4858 1305
Size: 3369 184 2007
Fragments arranged by size:
3369 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3738
Fragments arranged by size:
3738 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3518 3877 4036 518
Size: 648 2352 359 159 2042
Fragments arranged by size:
2352 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2976 3653 4801 5046 1606
Size: 1370 677 1148 245 2120
Fragments arranged by size:
2120 1370 1148 677 245
NaeI GCC'GGC
Cuts at: 2202 2202
Size: 5560
NarI GG'CG_CC
Cuts at: 2288 2288
Size: 5560
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4069
Fragments arranged by size:
4069 939 552
NdeI CA'TA_TG
Cuts at: 387 2769 387
Size: 2382 3178
NgoMIV G'CCGG_C
Cuts at: 2200 2200
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2791 2918 4463 1132
Size: 97 1562 127 1545 2229
Fragments arranged by size:
2229 1562 1545 127 97
PacI TTA_AT'TAA
Cuts at: 2841 2841
Size: 5560
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2053 1108
Size: 945 4615
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2187 2540 2912 830
Size: 1357 353 372 3478
Fragments arranged by size:
3478 1357 372 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2183 2536 2908 4043 4724 4915 826
Size: 219 1138 353 372 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 372 353 219 191
SfoI GGC'GCC
Cuts at: 2289 2289
Size: 5560
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2284 2887 1872
Size: 412 603 4545
Fragments arranged by size:
4545 603 412
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3664 4565 4827 820
Size: 197 926 654 1067 901 262 1553
Fragments arranged by size:
1553 1067 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2999 513
Size: 552 939 99 896 3074
Fragments arranged by size:
3074 939 896 552 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3587 3604 3757 3942 4387 325
Size: 1255 480 1527 17 153 185 445 1498
Fragments arranged by size:
1527 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 2225 1316
Size: 909 4651
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2503 2050
Size: 453 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2282 2885 1870
Size: 412 603 4545
Fragments arranged by size:
4545 603 412
XmnI GAAnn'nnTTC
Cuts at: 2337 2531 3689 2337
Size: 194 1158 4208
Fragments arranged by size:
4208 1158 194
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2122 3368 276
Size: 53 83 186 936 588 1246 2468
Fragments arranged by size:
2468 1246 936 588 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PacI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI