Restriction map for
pFN21AE2535
(Circular) (Six-base) MAPSORT of: pFN21AE2535.seq Check: 7021 from: 1 to: 5548
pFN21AE2535 5548 bp
With 182 enzymes: SgfI *
July 15, 2014 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2178 2178
Size: 5548
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3358 278
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2891 1937
Size: 954 4594
Acc65I G'GTAC_C
Cuts at: 2869 2869
Size: 5548
AclI AA'CG_TT
Cuts at: 1563 3675 4048 1563
Size: 2112 373 3063
Fragments arranged by size:
3063 2112 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 3611 4995 5263 1632
Size: 623 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 623 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5351
AflIII A'CryG_T
Cuts at: 1203 4447 5424 1203
Size: 3244 977 1327
Fragments arranged by size:
3244 1327 977
AgeI A'CCGG_T
Cuts at: 4379 4379
Size: 5548
AhdI GACnn_n'nnGTC
Cuts at: 4277 4277
Size: 5548
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2333 1184
Size: 1149 4399
AlwNI CAG_nnn'CTG
Cuts at: 2233 3291 4863 2233
Size: 1058 1572 2918
Fragments arranged by size:
2918 1572 1058
ApaLI G'TGCA_C
Cuts at: 3605 4761 3605
Size: 1156 4392
ApoI r'AATT_y
Cuts at: 1493 2857 3083 3235 5344 1493
Size: 1364 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4102 160
Size: 872 3070 1606
Fragments arranged by size:
3070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
AvaI C'yCGr_G
Cuts at: 1870 1943 2873 1870
Size: 73 930 4545
Fragments arranged by size:
4545 930 73
BamHI G'GATC_C
Cuts at: 2665 2707 2878 3245 2665
Size: 42 171 367 4968
Fragments arranged by size:
4968 367 171 42
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2150 2429 2869 4324
Size: 325 217 627 255 108 279 440 1455
Cuts at: 4324 618
Size: 1842
Fragments arranged by size:
1842 1455 627 440 325 279 255 217
108
BanII G_rGCy'C
Cuts at: 1391 2261 2867 1391
Size: 870 606 4072
Fragments arranged by size:
4072 870 606
BbeI G_GCGC'C
Cuts at: 2154 2154
Size: 5548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2011 2021 2045 3739 3773 1903
Size: 34 50 24 10 24 1694 34 3678
Fragments arranged by size:
3678 1694 50 34 34 24 24 10
BciVI GTATCCnnnnn_n'
Cuts at: 3440 4650 3440
Size: 1210 4338
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5548
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2148 2228 2609
Size: 107 122 71 631 605 476 80 381
Cuts at: 2609 4159 136
Size: 1550 1525
Fragments arranged by size:
1550 1525 631 605 476 381 122 107
80 71
BglII A'GATC_T
Cuts at: 5543 5543
Size: 5548
BlpI GC'TnA_GC
Cuts at: 2965 2965
Size: 5548
Bme1580I G_kGCm'C
Cuts at: 1790 2045 3609 4765 1790
Size: 255 1564 1156 2573
Fragments arranged by size:
2573 1564 1156 255
BmgBI CAC'GTC
Cuts at: 1318 2219 1318
Size: 901 4647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3279 4237 442
Size: 416 53 83 399 24 1862 958 1753
Fragments arranged by size:
1862 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2720 4208 1239
Size: 980 501 1488 2579
Fragments arranged by size:
2579 1488 980 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 3673 4538 4836 2336
Size: 1337 865 298 3048
Fragments arranged by size:
3048 1337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4211 882
Size: 617 2712 2219
Fragments arranged by size:
2712 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3339 493
Size: 711 2135 2702
Fragments arranged by size:
2702 2135 711
BsaBI GATnn'nnATC
Cuts at: 5542 5542
Size: 5548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 2391 3355 3737
Size: 53 83 186 936 618 240 964 382
Cuts at: 3737 4372 275
Size: 635 1451
Fragments arranged by size:
1451 964 936 635 618 382 240 186
83 53
BsaWI w'CCGG_w
Cuts at: 3981 4379 4653 4800 5211 3981
Size: 398 274 147 411 4318
Fragments arranged by size:
4318 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5323
Fragments arranged by size:
5323 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2717 1186
Size: 1531 4017
BseYI C'CCAG_C
Cuts at: 2068 2328 2422 4751 5247 2068
Size: 260 94 2329 496 2369
Fragments arranged by size:
2369 2329 496 260 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2150 2659 1163
Size: 573 297 117 509 4052
Fragments arranged by size:
4052 573 509 297 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3759 4787 1540
Size: 275 186 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 3609 3694 4765 2205
Size: 1404 85 1071 2988
Fragments arranged by size:
2988 1404 1071 85
BsmI GAATG_Cn'
Cuts at: 3059 3152 3059
Size: 93 5455
BsmBI CGTCTCn'nnnn_
Cuts at: 2483 2483
Size: 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2205 2261 2867 3609 3694 4765
Size: 399 255 160 56 606 742 85 1071
Cuts at: 4765 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 606 399 255 160 85
56
BspEI T'CCGG_A
Cuts at: 5211 5211
Size: 5548
BspHI T'CATG_A
Cuts at: 3437 5167 3437
Size: 1730 3818
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2836 2903 844
Size: 1334 658 67 3489
Fragments arranged by size:
3489 1334 658 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3435 1394
Size: 429 1612 3507
Fragments arranged by size:
3507 1612 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 4043 4217 65
Size: 1183 1026 1769 174 1396
Fragments arranged by size:
1769 1396 1183 1026 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2205 2222 2441 4192 4379 1700
Size: 110 198 197 17 219 1751 187 2869
Fragments arranged by size:
2869 1751 219 198 197 187 110 17
BsrGI T'GTAC_A
Cuts at: 96 2595 96
Size: 2499 3049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5548
BssSI C'ACGA_G
Cuts at: 2414 2554 3608 4620 2414
Size: 140 1054 1012 3342
Fragments arranged by size:
3342 1054 1012 140
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5548
BstBI TT'CG_AA
Cuts at: 4352 4352
Size: 5548
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5548
BstYI r'GATC_y
Cuts at: 1972 2665 2707 2878 3245 3630 3647 5099 5185
Size: 693 42 171 367 385 17 1452 86
Cuts at: 5185 5197 5543 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 693 385 367 346 171 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2249 513
Size: 552 1184 3812
Fragments arranged by size:
3812 1184 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4809
Fragments arranged by size:
4809 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 3064 3858 3878 2070
Size: 994 794 20 3740
Fragments arranged by size:
3740 994 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5513
DraI TTT'AAA
Cuts at: 2852 2852
Size: 5548
DraIII CAC_nnn'GTG
Cuts at: 2419 2419
Size: 5548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4555 809
Size: 507 3239 1802
Fragments arranged by size:
3239 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 2995 3884
Size: 54 1425 50 275 18 394 771 889
Cuts at: 3884 5513 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 771 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 1537
Size: 275 1183 4090
Fragments arranged by size:
4090 1183 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3478 1353
Size: 2125 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4135 4509 4655 387
Size: 1064 138 2546 374 146 1280
Fragments arranged by size:
2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2255 2720 3611 4208 4995 5263
Size: 393 587 36 465 891 597 787 268
Cuts at: 5263 1239
Size: 1524
Fragments arranged by size:
1524 891 787 597 587 465 393 268
36
EcoNI CCTnn'n_nnAGG
Cuts at: 2507 2702 2507
Size: 195 5353
EcoO109I rG'GnC_Cy
Cuts at: 2230 2293 2611 2230
Size: 63 318 5167
Fragments arranged by size:
5167 318 63
EcoRI G'AATT_C
Cuts at: 2857 3235 2857
Size: 378 5170
FspI TGC'GCA
Cuts at: 2309 4054 2309
Size: 1745 3803
HaeII r_GCGC'y
Cuts at: 1678 2154 4695 1678
Size: 476 2541 2531
Fragments arranged by size:
2541 2531 476
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373
Size: 32 135 32 2617 32 42 32 2626
Fragments arranged by size:
2626 2617 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2892 3138 669
Size: 988 281 954 246 3079
Fragments arranged by size:
3079 988 954 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5548
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 5548
KasI G'GCGC_C
Cuts at: 2150 2150
Size: 5548
KpnI G_GTAC'C
Cuts at: 2873 2873
Size: 5548
MfeI C'AATT_G
Cuts at: 3147 3147
Size: 5548
MluI A'CGCG_T
Cuts at: 5424 5424
Size: 5548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4662 4846 1305
Size: 3357 184 2007
Fragments arranged by size:
3357 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3726
Fragments arranged by size:
3726 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2366 2639 2684 3506 3865 4024
Size: 648 843 357 273 45 822 359 159
Cuts at: 4024 518
Size: 2042
Fragments arranged by size:
2042 843 822 648 359 357 273 159
45
MspA1I CmG'CkG
Cuts at: 1606 2017 2251 2964 3641 4789 5034 1606
Size: 411 234 713 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 411 245 234
NaeI GCC'GGC
Cuts at: 2224 2443 2224
Size: 219 5329
NarI GG'CG_CC
Cuts at: 2151 2151
Size: 5548
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4996
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5548
NgoMIV G'CCGG_C
Cuts at: 2222 2441 2222
Size: 219 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5548
NotI GC'GGCC_GC
Cuts at: 2995 2995
Size: 5548
NspI r_CATG'y
Cuts at: 1132 1229 2215 2401 2906 4451 1132
Size: 97 986 186 505 1545 2229
Fragments arranged by size:
2229 1545 986 505 186 97
PasI CC'CwG_GG
Cuts at: 1348 2747 1348
Size: 1399 4149
PciI A'CATG_T
Cuts at: 4447 4447
Size: 5548
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5548
PfoI T'CCnGG_A
Cuts at: 2196 2697 2196
Size: 501 5047
PmeI GTTT'AAAC
Cuts at: 2852 2852
Size: 5548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3617 3649 5130 5162 3617
Size: 32 1481 32 4003
Fragments arranged by size:
4003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2293
Size: 5548
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5548
PsiI TTA'TAA
Cuts at: 3118 3118
Size: 5548
PstI C_TGCA'G
Cuts at: 830 2508 2900 830
Size: 1678 392 3478
Fragments arranged by size:
3478 1678 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5548
PvuII CAG'CTG
Cuts at: 2017 2017
Size: 5548
SacII CC_GC'GG
Cuts at: 2252 2252
Size: 5548
SalI G'TCGA_C
Cuts at: 1936 2890 1936
Size: 954 4594
SbfI CC_TGCA'GG
Cuts at: 2508 2900 2508
Size: 392 5156
ScaI AGT'ACT
Cuts at: 3796 5508 3796
Size: 1712 3836
SexAI A'CCwGG_T
Cuts at: 2775 2775
Size: 5548
SfcI C'TryA_G
Cuts at: 826 1045 2504 2896 4031 4712 4903 826
Size: 219 1459 392 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 681 392 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2609 2609
Size: 5548
SfoI GGC'GCC
Cuts at: 2152 2152
Size: 5548
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5548
SgrAI Cr'CCGG_yG
Cuts at: 1700 2205 1700
Size: 505 5043
SmaI CCC'GGG
Cuts at: 1872 2875 1872
Size: 1003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 3652 4553 4815 820
Size: 197 926 408 1301 901 262 1553
Fragments arranged by size:
1553 1301 926 901 408 262 197
SnaBI TAC'GTA
Cuts at: 493 3339 493
Size: 2846 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5548
SphI G_CATG'C
Cuts at: 1132 2215 2906 1132
Size: 1083 691 3774
Fragments arranged by size:
3774 1083 691
SspI AAT'ATT
Cuts at: 5 52 3472 5
Size: 47 3420 2081
Fragments arranged by size:
3420 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5548
StyI C'CwwG_G
Cuts at: 513 1065 2987 513
Size: 552 1922 3074
Fragments arranged by size:
3074 1922 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3575 3592 3745 3930 4375 325
Size: 1255 480 1515 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3794 5506
Size: 274 80 33 51 1443 618 1199 1712
Cuts at: 5506 96
Size: 138
Fragments arranged by size:
1712 1443 1199 618 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 5508 5540 2117
Size: 32 3359 32 2125
Fragments arranged by size:
3359 2125 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2777 1316
Size: 1461 4087
XbaI T'CTAG_A
Cuts at: 2837 2884 2837
Size: 47 5501
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5548
XmaI C'CCGG_G
Cuts at: 1870 2873 1870
Size: 1003 4545
XmnI GAAnn'nnTTC
Cuts at: 3677 3677
Size: 5548
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3356 276
Size: 53 83 186 936 1822 2468
Fragments arranged by size:
2468 1822 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SanDI SapI SrfI SwaI
XcmI