Restriction map for
pFN21AE2531
(Circular) (Six-base) MAPSORT of: pFN21AE2531.seq Check: 3127 from: 1 to: 6808
pFN21AE2531 6808 bp
With 182 enzymes: SgfI *
July 15, 2014 17:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3879 3879
Size: 6808
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4618 278
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4151 1937
Size: 2214 4594
Acc65I G'GTAC_C
Cuts at: 4129 4129
Size: 6808
AclI AA'CG_TT
Cuts at: 1563 2293 4935 5308 1563
Size: 730 2642 373 3063
Fragments arranged by size:
3063 2642 730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2607 4871 6255 6523 1632
Size: 735 240 2264 1384 268 1917
Fragments arranged by size:
2264 1917 1384 735 268 240
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 2309 5707 6684 1203
Size: 1106 3398 977 1327
Fragments arranged by size:
3398 1327 1106 977
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 5537 5537
Size: 6808
AleI CACnn'nnGTG
Cuts at: 1166 2978 1166
Size: 1812 4996
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6808
AlwNI CAG_nnn'CTG
Cuts at: 2373 3025 4551 6123 2373
Size: 652 1526 1572 3058
Fragments arranged by size:
3058 1572 1526 652
ApaLI G'TGCA_C
Cuts at: 4865 6021 4865
Size: 1156 5652
ApoI r'AATT_y
Cuts at: 1493 2188 2541 3310 4078 4117 4343 4495 6604
Size: 695 353 769 768 39 226 152 2109
Cuts at: 6604 1493
Size: 1697
Fragments arranged by size:
2109 1697 769 768 695 353 226 152
39
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 3951 4133 1870
Size: 73 2008 182 4545
Fragments arranged by size:
4545 2008 182 73
BamHI G'GATC_C
Cuts at: 4138 4505 4138
Size: 367 6441
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3282 3891 4129 5584 618
Size: 325 217 627 1495 609 238 1455 1842
Fragments arranged by size:
1842 1495 1455 627 609 325 238 217
BanII G_rGCy'C
Cuts at: 1391 4067 4127 1391
Size: 2676 60 4072
Fragments arranged by size:
4072 2676 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2373 3768 3912 928
Size: 971 474 1395 144 3824
Fragments arranged by size:
3824 1395 971 474 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4999 5033 1903
Size: 34 3062 34 3678
Fragments arranged by size:
3678 3062 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4700 5910 4700
Size: 1210 5598
BclI T'GATC_A
Cuts at: 1548 2896 3061 3460 4008 1548
Size: 1348 165 399 548 4348
Fragments arranged by size:
4348 1348 548 399 165
BfrBI ATG'CAT
Cuts at: 2285 2941 2285
Size: 656 6152
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5419 136
Size: 107 122 71 631 605 3747 1525
Fragments arranged by size:
3747 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6803 6803
Size: 6808
BlpI GC'TnA_GC
Cuts at: 4225 4225
Size: 6808
Bme1580I G_kGCm'C
Cuts at: 1790 2179 3287 4869 6025 1790
Size: 389 1108 1582 1156 2573
Fragments arranged by size:
2573 1582 1156 1108 389
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6808
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3159 3248 4539
Size: 416 53 83 399 24 1742 89 1291
Cuts at: 4539 5497 442
Size: 958 1753
Fragments arranged by size:
1753 1742 1291 958 416 399 89 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6808
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2294 2294
Size: 6808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 2355 3468 5468 1239
Size: 819 297 1113 2000 2579
Fragments arranged by size:
2579 2000 1113 819 297
Bpu10I CC'TnA_GC
Cuts at: 2265 2265
Size: 6808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2800 4933 5798 6096 2800
Size: 2133 865 298 3512
Fragments arranged by size:
3512 2133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5471 882
Size: 617 3972 2219
Fragments arranged by size:
3972 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4599 493
Size: 711 3395 2702
Fragments arranged by size:
3395 2702 711
BsaBI GATnn'nnATC
Cuts at: 3516 4007 6802 3516
Size: 491 2795 3522
Fragments arranged by size:
3522 2795 491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4615 4997 5632 275
Size: 53 83 186 936 3082 382 635 1451
Fragments arranged by size:
3082 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5241 5639 5913 6060 6471 5241
Size: 398 274 147 411 5578
Fragments arranged by size:
5578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3723 3753 1178
Size: 30 165 30 2320 30 4233
Fragments arranged by size:
4233 2320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2761 1186
Size: 1575 5233
BseYI C'CCAG_C
Cuts at: 6011 6507 6011
Size: 496 6312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3844 3907 1163
Size: 573 2108 63 4064
Fragments arranged by size:
4064 2108 573 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4258 5019 6047 1540
Size: 275 186 2257 761 1028 2301
Fragments arranged by size:
2301 2257 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2562 4067 4869 4954 6025 2562
Size: 1505 802 85 1071 3345
Fragments arranged by size:
3345 1505 1071 802 85
BsmI GAATG_Cn'
Cuts at: 2266 2648 4319 4412 2266
Size: 382 1671 93 4662
Fragments arranged by size:
4662 1671 382 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2562 3287 4067 4127 4869 4954
Size: 399 389 383 725 780 60 742 85
Cuts at: 4954 6025 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 780 742 725 399 389 383
85 60
BspEI T'CCGG_A
Cuts at: 6471 6471
Size: 6808
BspHI T'CATG_A
Cuts at: 4697 6427 4697
Size: 1730 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3879 4163 844
Size: 3035 284 3489
Fragments arranged by size:
3489 3035 284
BsrBI CCG'CTC
Cuts at: 1394 1823 4695 1394
Size: 429 2872 3507
Fragments arranged by size:
3507 2872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3966 5303 5477 65
Size: 1183 2718 1337 174 1396
Fragments arranged by size:
2718 1396 1337 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5452 5639 1700
Size: 110 3642 187 2869
Fragments arranged by size:
3642 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6808
BssSI C'ACGA_G
Cuts at: 2051 2459 4868 5880 2051
Size: 408 2409 1012 2979
Fragments arranged by size:
2979 2409 1012 408
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2373 794
Size: 1579 5229
BstBI TT'CG_AA
Cuts at: 5612 5612
Size: 6808
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6808
BstXI CCAn_nnnn'nTGG
Cuts at: 2927 3483 2927
Size: 556 6252
BstYI r'GATC_y
Cuts at: 1972 2143 4138 4505 4890 4907 6359 6445 6457
Size: 171 1995 367 385 17 1452 86 12
Cuts at: 6457 6803 1972
Size: 346 1977
Fragments arranged by size:
1995 1977 1452 385 367 346 171 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5317
Fragments arranged by size:
5317 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6069
Fragments arranged by size:
6069 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 2985 3058 4324 5118 5138 2374
Size: 611 73 1266 794 20 4044
Fragments arranged by size:
4044 1266 794 611 73 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6808
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2350 2385 3452 3487 529
Size: 35 1786 35 1067 35 3850
Fragments arranged by size:
3850 1786 1067 35 35 35
DraI TTT'AAA
Cuts at: 4112 4112
Size: 6808
DraIII CAC_nnn'GTG
Cuts at: 3242 3242
Size: 6808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3962 5815 809
Size: 507 2646 1853 1802
Fragments arranged by size:
2646 1853 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2710 3473 4255
Size: 54 1425 50 275 18 880 763 782
Cuts at: 4255 5144 6773 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 880 782 763 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4255 1537
Size: 275 2443 4090
Fragments arranged by size:
4090 2443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2779 3373 3655 4738 1353
Size: 699 727 594 282 1083 3423
Fragments arranged by size:
3423 1083 727 699 594 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2112 5395 5769 5915 387
Size: 1064 138 523 3283 374 146 1280
Fragments arranged by size:
3283 1280 1064 523 374 146 138
EcoICRI GAG'CTC
Cuts at: 4065 4065
Size: 6808
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2355 2367 2607 3468 4871 5468
Size: 393 426 297 12 240 861 1403 597
Cuts at: 5468 6255 6523 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1403 861 787 597 426 393 297
268 240 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3009 3009
Size: 6808
EcoRI G'AATT_C
Cuts at: 2541 4117 4495 2541
Size: 1576 378 4854
Fragments arranged by size:
4854 1576 378
EcoRV GAT'ATC
Cuts at: 2122 2122
Size: 6808
FspI TGC'GCA
Cuts at: 5314 5314
Size: 6808
HaeII r_GCGC'y
Cuts at: 1678 5955 1678
Size: 4277 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 2939 2971 3812
Size: 32 135 32 1001 32 334 32 841
Cuts at: 3812 3844 4054 4086 5449 5481 5523 5555 1373
Size: 32 210 32 1363 32 42 32 2626
Fragments arranged by size:
2626 1363 1001 841 334 210 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4152 4398 669
Size: 988 281 2214 246 3079
Fragments arranged by size:
3079 2214 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6808
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KpnI G_GTAC'C
Cuts at: 4133 4133
Size: 6808
MfeI C'AATT_G
Cuts at: 2673 4407 2673
Size: 1734 5074
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2928 5922 6106 1305
Size: 1623 2994 184 2007
Fragments arranged by size:
2994 2007 1623 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3475 10
Size: 54 1425 343 1643 3343
Fragments arranged by size:
3343 1643 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2978 4766 5125 5284 518
Size: 648 1812 1788 359 159 2042
Fragments arranged by size:
2042 1812 1788 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3550 4224 4901 6049 6294 1606
Size: 1944 674 677 1148 245 2120
Fragments arranged by size:
2120 1944 1148 677 674 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5317
Fragments arranged by size:
5317 939 552
NdeI CA'TA_TG
Cuts at: 387 2233 387
Size: 1846 4962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6808
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NsiI A_TGCA'T
Cuts at: 2287 2943 2287
Size: 656 6152
NspI r_CATG'y
Cuts at: 1132 1229 2313 4103 4166 5711 1132
Size: 97 1084 1790 63 1545 2229
Fragments arranged by size:
2229 1790 1545 1084 97 63
PasI CC'CwG_GG
Cuts at: 1348 2880 1348
Size: 1532 5276
PciI A'CATG_T
Cuts at: 2309 5707 2309
Size: 3398 3410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2476 2719 1108
Size: 1368 243 5197
Fragments arranged by size:
5197 1368 243
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 6390 6422 4877
Size: 32 1481 32 5263
Fragments arranged by size:
5263 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2675 1613
Size: 1062 5746
PsiI TTA'TAA
Cuts at: 3692 3725 4378 3692
Size: 33 653 6122
Fragments arranged by size:
6122 653 33
PstI C_TGCA'G
Cuts at: 830 3097 4160 830
Size: 2267 1063 3478
Fragments arranged by size:
3478 2267 1063
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
SacI G_AGCT'C
Cuts at: 4067 4067
Size: 6808
SalI G'TCGA_C
Cuts at: 1936 4150 1936
Size: 2214 4594
SapI GCTCTTCn'nnn_
Cuts at: 2052 2052
Size: 6808
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 5056 6768 5056
Size: 1712 5096
SfcI C'TryA_G
Cuts at: 826 1045 2575 2635 3093 3561 4156 5291 5972
Size: 219 1530 60 458 468 595 1135 681
Cuts at: 5972 6163 826
Size: 191 1471
Fragments arranged by size:
1530 1471 1135 681 595 468 458 219
191 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 4135 1872
Size: 2263 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2779 4912 5813 6075 820
Size: 197 926 836 2133 901 262 1553
Fragments arranged by size:
2133 1553 926 901 836 262 197
SnaBI TAC'GTA
Cuts at: 493 4599 493
Size: 4106 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 4103 4166 1132
Size: 2971 63 3774
Fragments arranged by size:
3774 2971 63
SspI AAT'ATT
Cuts at: 5 52 4732 5
Size: 47 4680 2081
Fragments arranged by size:
4680 2081 47
StuI AGG'CCT
Cuts at: 1517 3610 1517
Size: 2093 4715
StyI C'CwwG_G
Cuts at: 513 1065 2004 2327 2920 3482 4247 513
Size: 552 939 323 593 562 765 3074
Fragments arranged by size:
3074 939 765 593 562 552 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2454 3230 4835 4852 5005 5190 5635
Size: 1255 874 776 1605 17 153 185 445
Cuts at: 5635 325
Size: 1498
Fragments arranged by size:
1605 1498 1255 874 776 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5054 6766 96
Size: 274 80 33 51 1443 3077 1712 138
Fragments arranged by size:
3077 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2804 2836 3266 3298 3896 3928 6768 6800 2804
Size: 32 430 32 598 32 2840 32 2812
Fragments arranged by size:
2840 2812 598 430 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2519 3854 1316
Size: 1203 1335 4270
Fragments arranged by size:
4270 1335 1203
XbaI T'CTAG_A
Cuts at: 2239 4144 2239
Size: 1905 4903
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 3443 2980
Size: 463 6345
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6808
XmaI C'CCGG_G
Cuts at: 1870 4133 1870
Size: 2263 4545
XmnI GAAnn'nnTTC
Cuts at: 4937 4937
Size: 6808
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4616 276
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I Bsu36I EcoO109I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI