Restriction map for
pFN21AE2530
(Circular) (Six-base) MAPSORT of: pFN21AE2530.seq Check: 7293 from: 1 to: 14071
pFN21AE2530 14071 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2389 2389
Size: 14071
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4311 8795 11881 278
Size: 53 83 186 936 2775 4484 3086 2468
Fragments arranged by size:
4484 3086 2775 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4193 11414 1937
Size: 2256 7221 4594
Fragments arranged by size:
7221 4594 2256
Acc65I G'GTAC_C
Cuts at: 8691 9075 11392 8691
Size: 384 2317 11370
Fragments arranged by size:
11370 2317 384
AclI AA'CG_TT
Cuts at: 1563 4830 11370 12198 12571 1563
Size: 3267 6540 828 373 3063
Fragments arranged by size:
6540 3267 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3075 3181 3963 4081 4428 5553 5588 7139
Size: 1443 106 782 118 347 1125 35 1551
Cuts at: 7139 7245 7901 7912 8219 8436 9491 10462 11297
Size: 106 656 11 307 217 1055 971 835
Cuts at: 11297 12134 13518 13786 1632
Size: 837 1384 268 1917
Fragments arranged by size:
1917 1551 1443 1384 1125 1055 971 837
835 782 656 347 307 268 217 118
106 106 35 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 13874
AflIII A'CryG_T
Cuts at: 1203 2174 2218 2942 3179 3859 8650 8887 10802
Size: 971 44 724 237 680 4791 237 1915
Cuts at: 10802 12970 13947 1203
Size: 2168 977 1327
Fragments arranged by size:
4791 2168 1915 1327 977 971 724 680
237 237 44
AgeI A'CCGG_T
Cuts at: 10844 12902 10844
Size: 2058 12013
AhdI GACnn_n'nnGTC
Cuts at: 4648 10248 12800 4648
Size: 5600 2552 5919
Fragments arranged by size:
5919 5600 2552
AleI CACnn'nnGTG
Cuts at: 1166 4344 1166
Size: 3178 10893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2526 5895 6711 1184
Size: 1342 3369 816 8544
Fragments arranged by size:
8544 3369 1342 816
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3849 3881 10603 10635 3849
Size: 32 6722 32 7285
Fragments arranged by size:
7285 6722 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4163 5499 7076 11814 13386 4163
Size: 1336 1577 4738 1572 4848
Fragments arranged by size:
4848 4738 1577 1572 1336
ApaI G_GGCC'C
Cuts at: 6889 6889
Size: 14071
ApaLI G'TGCA_C
Cuts at: 5136 5404 6736 12128 13284 5136
Size: 268 1332 5392 1156 5923
Fragments arranged by size:
5923 5392 1332 1156 268
ApoI r'AATT_y
Cuts at: 1493 2705 4022 4042 5255 5459 6043 7043 7141
Size: 1212 1317 20 1213 204 584 1000 98
Cuts at: 7141 8305 8573 8953 9291 9504 10007 10581 10898
Size: 1164 268 380 338 213 503 574 317
Cuts at: 10898 10904 11380 11606 11758 13867 1493
Size: 6 476 226 152 2109 1697
Fragments arranged by size:
2109 1697 1317 1213 1212 1164 1000 584
574 503 476 380 338 317 268 226
213 204 152 98 20 6
AseI AT'TA_AT
Cuts at: 160 1032 3141 12625 160
Size: 872 2109 9484 1606
Fragments arranged by size:
9484 2109 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14071
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 5787 5958 5979 7062 7119 7287
Size: 73 111 3733 171 21 1083 57 168
Cuts at: 7287 7881 11396 1870
Size: 594 3515 4545
Fragments arranged by size:
4545 3733 3515 1083 594 171 168 111
73 57 21
AvrII C'CTAG_G
Cuts at: 4223 9725 4223
Size: 5502 8569
BamHI G'GATC_C
Cuts at: 2745 3323 3442 5201 11401 11768 2745
Size: 578 119 1759 6200 367 5048
Fragments arranged by size:
6200 5048 1759 578 367 119
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4962 6248 8691 9075
Size: 325 217 627 1529 1646 1286 2443 384
Cuts at: 9075 11392 12847 618
Size: 2317 1455 1842
Fragments arranged by size:
2443 2317 1842 1646 1529 1455 1286 627
384 325 217
BanII G_rGCy'C
Cuts at: 1391 4109 4704 6889 8178 8746 9183 9221 9621
Size: 2718 595 2185 1289 568 437 38 400
Cuts at: 9621 11121 11390 1391
Size: 1500 269 4072
Fragments arranged by size:
4072 2718 2185 1500 1289 595 568 437
400 269 38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4361 4416 5991 7581 7907 7986 8442
Size: 971 2462 55 1575 1590 326 79 456
Cuts at: 8442 9354 928
Size: 912 5645
Fragments arranged by size:
5645 2462 1590 1575 971 912 456 326
79 55
BbvCI CC'TCA_GC
Cuts at: 3696 6879 3696
Size: 3183 10888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5079 5113 8273 8307 10282
Size: 34 50 34 3058 34 3160 34 1975
Cuts at: 10282 10316 12262 12296 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 3160 3058 1975 1946 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6359 11963 13173 6359
Size: 5604 1210 7257
Fragments arranged by size:
7257 5604 1210
BclI T'GATC_A
Cuts at: 1548 2959 5389 5826 9834 1548
Size: 1411 2430 437 4008 5785
Fragments arranged by size:
5785 4008 2430 1411 437
BfrBI ATG'CAT
Cuts at: 5094 9050 5094
Size: 3956 10115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2058 5454 12682
Size: 107 122 71 631 605 386 3396 7228
Cuts at: 12682 136
Size: 1525
Fragments arranged by size:
7228 3396 1525 631 605 386 122 107
71
BglII A'GATC_T
Cuts at: 5683 7843 14066 5683
Size: 2160 6223 5688
Fragments arranged by size:
6223 5688 2160
BlpI GC'TnA_GC
Cuts at: 5574 11488 5574
Size: 5914 8157
Bme1580I G_kGCm'C
Cuts at: 1790 4184 5005 5140 5408 6251 6535 6618 6740
Size: 2394 821 135 268 843 284 83 122
Cuts at: 6740 6889 8775 9195 9814 10138 12132 13288 1790
Size: 149 1886 420 619 324 1994 1156 2573
Fragments arranged by size:
2573 2394 1994 1886 1156 843 821 619
420 324 284 268 149 135 122 83
BmgBI CAC'GTC
Cuts at: 1318 5556 6101 8651 1318
Size: 4238 545 2550 6738
Fragments arranged by size:
6738 4238 2550 545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2337 2571 5094
Size: 416 53 83 399 24 920 234 2523
Cuts at: 5094 5406 5518 8724 8868 10229 11802 12760 442
Size: 312 112 3206 144 1361 1573 958 1753
Fragments arranged by size:
3206 2523 1753 1573 1361 958 920 416
399 312 234 144 112 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 14071
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5702 5702
Size: 14071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2676 3013 3775 3851 4335 4599 4992 6105
Size: 1437 337 762 76 484 264 393 1113
Cuts at: 6105 6585 6675 6791 8661 8784 9593 10158 10768
Size: 480 90 116 1870 123 809 565 610
Cuts at: 10768 10947 11327 12731 1239
Size: 179 380 1404 2579
Fragments arranged by size:
2579 1870 1437 1404 1113 809 762 610
565 484 480 393 380 337 264 179
123 116 90 76
Bpu10I CC'TnA_GC
Cuts at: 3696 6879 9220 9600 10197 3696
Size: 3183 2341 380 597 7570
Fragments arranged by size:
7570 3183 2341 597 380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3919 5747 5805 8062 8381 9460 11207 12196 13061
Size: 1828 58 2257 319 1079 1747 989 865
Cuts at: 13061 13359 3919
Size: 298 4631
Fragments arranged by size:
4631 2257 1828 1747 1079 989 865 319
298 58
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2662 9147 12734 882
Size: 617 1163 6485 3587 2219
Fragments arranged by size:
6485 3587 2219 1163 617
BsaAI yAC'GTr
Cuts at: 493 1204 3110 6645 8888 11862 493
Size: 711 1906 3535 2243 2974 2702
Fragments arranged by size:
3535 2974 2702 2243 1906 711
BsaBI GATnn'nnATC
Cuts at: 2648 2864 3707 9333 14065 2648
Size: 216 843 5626 4732 2654
Fragments arranged by size:
5626 4732 2654 843 216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4308 8792 11878 12260
Size: 53 83 186 936 2775 4484 3086 382
Cuts at: 12260 12895 275
Size: 635 1451
Fragments arranged by size:
4484 3086 2775 1451 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2045 4479 5550 6995 10844 12504 12902 13176 13323
Size: 2434 1071 1445 3849 1660 398 274 147
Cuts at: 13323 13734 2045
Size: 411 2382
Fragments arranged by size:
3849 2434 2382 1660 1445 1071 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2659 2689 3117 3147 3849
Size: 30 165 30 1256 30 428 30 702
Cuts at: 3849 3879 4716 4746 6100 6130 8782 8812 1178
Size: 30 837 30 1354 30 2652 30 6437
Fragments arranged by size:
6437 2652 1354 1256 837 702 428 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 3501 4192 5874 6108 7204 7622 8540
Size: 832 1483 691 1682 234 1096 418 918
Cuts at: 8540 8787 9356 9761 1186
Size: 247 569 405 5496
Fragments arranged by size:
5496 1682 1483 1096 918 832 691 569
418 405 247 234
BseYI C'CCAG_C
Cuts at: 3672 3790 4862 6708 8774 10213 11045 11161 11362
Size: 118 1072 1846 2066 1439 832 116 201
Cuts at: 11362 13274 13770 3672
Size: 1912 496 3973
Fragments arranged by size:
3973 2066 1912 1846 1439 1072 832 496
201 118 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2584 3917 4566 4840 5020 5302 6469
Size: 573 848 1333 649 274 180 282 1167
Cuts at: 6469 6625 1163
Size: 156 8609
Fragments arranged by size:
8609 1333 1167 848 649 573 282 274
180 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2078 11521 12282 13310 1540
Size: 275 186 77 9443 761 1028 2301
Fragments arranged by size:
9443 2301 1028 761 275 186 77
BsiHKAI G_wGCw'C
Cuts at: 4109 4704 5140 5408 5531 6361 6740 8178 9130
Size: 595 436 268 123 830 379 1438 952
Cuts at: 9130 9183 9621 10000 11121 12132 12217 13288 4109
Size: 53 438 379 1121 1011 85 1071 4892
Fragments arranged by size:
4892 1438 1121 1071 1011 952 830 595
438 436 379 379 268 123 85 53
BsmI GAATG_Cn'
Cuts at: 2764 3065 3657 4325 8248 8466 10659 11582 11675
Size: 301 592 668 3923 218 2193 923 93
Cuts at: 11675 2764
Size: 5160
Fragments arranged by size:
5160 3923 2193 923 668 592 301 218
93
BsmBI CGTCTCn'nnnn_
Cuts at: 7116 8799 10367 7116
Size: 1683 1568 10820
Fragments arranged by size:
10820 1683 1568
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4109 4184 4704 5005 5140 5408 5531
Size: 399 2319 75 520 301 135 268 123
Cuts at: 5531 6251 6361 6535 6618 6740 6889 8178 8746
Size: 720 110 174 83 122 149 1289 568
Cuts at: 8746 8775 9130 9183 9195 9221 9621 9814 10000
Size: 29 355 53 12 26 400 193 186
Cuts at: 10000 10138 11121 11390 12132 12217 13288 1391
Size: 138 983 269 742 85 1071 2174
Fragments arranged by size:
2319 2174 1289 1071 983 742 720 568
520 400 399 355 301 269 268 193
186 174 149 138 135 123 122 110
85 83 75 53 29 26 12
BspEI T'CCGG_A
Cuts at: 2045 5550 13734 2045
Size: 3505 8184 2382
Fragments arranged by size:
8184 3505 2382
BspHI T'CATG_A
Cuts at: 6783 9168 9859 11960 13690 6783
Size: 2385 691 2101 1730 7164
Fragments arranged by size:
7164 2385 2101 1730 691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2389 3939 11426 844
Size: 1545 1550 7487 3489
Fragments arranged by size:
7487 3489 1550 1545
BsrBI CCG'CTC
Cuts at: 1394 1823 2717 11958 1394
Size: 429 894 9241 3507
Fragments arranged by size:
9241 3507 894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3247 3331 4152 4612 5227 6413 7516
Size: 1183 1999 84 821 460 615 1186 1103
Cuts at: 7516 8277 8841 10323 12566 12740 65
Size: 761 564 1482 2243 174 1396
Fragments arranged by size:
2243 1999 1482 1396 1186 1183 1103 821
761 615 564 460 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 3763 4689 4769 5448 8325 10844 12715
Size: 110 1953 926 80 679 2877 2519 1871
Cuts at: 12715 12902 1700
Size: 187 2869
Fragments arranged by size:
2877 2869 2519 1953 1871 926 679 187
110 80
BsrGI T'GTAC_A
Cuts at: 96 5273 96
Size: 5177 8894
BssHII G'CGCG_C
Cuts at: 1925 3759 1925
Size: 1834 12237
BssSI C'ACGA_G
Cuts at: 6932 9150 12131 13143 6932
Size: 2218 2981 1012 7860
Fragments arranged by size:
7860 2981 2218 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3736 4736 5499 8985 794
Size: 2942 1000 763 3486 5880
Fragments arranged by size:
5880 3486 2942 1000 763
BstBI TT'CG_AA
Cuts at: 8470 12875 8470
Size: 4405 9666
BstEII G'GTnAC_C
Cuts at: 1184 10671 1184
Size: 9487 4584
BstXI CCAn_nnnn'nTGG
Cuts at: 7991 9260 11329 7991
Size: 1269 2069 10733
Fragments arranged by size:
10733 2069 1269
BstYI r'GATC_y
Cuts at: 1972 2745 3323 3442 5201 5683 7315 7843 8035
Size: 773 578 119 1759 482 1632 528 192
Cuts at: 8035 8257 8636 11270 11401 11768 12153 12170 13622
Size: 222 379 2634 131 367 385 17 1452
Cuts at: 13622 13708 13720 14066 1972
Size: 86 12 346 1977
Fragments arranged by size:
2634 1977 1759 1632 1452 773 578 528
482 385 379 367 346 222 192 131
119 86 17 12
BstZ17I GTA'TAC
Cuts at: 4194 4194
Size: 14071
Bsu36I CC'TnA_GG
Cuts at: 9000 9000
Size: 14071
BtgI C'CryG_G
Cuts at: 513 1065 5005 6497 6660 10083 513
Size: 552 3940 1492 163 3423 4501
Fragments arranged by size:
4501 3940 3423 1492 552 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3564 5104 487
Size: 683 56 2338 1540 9454
Fragments arranged by size:
9454 2338 1540 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 3156 3424 5408 6330 6605 7767 11028 11088
Size: 901 268 1984 922 275 1162 3261 60
Cuts at: 11088 11587 12381 12401 2255
Size: 499 794 20 3925
Fragments arranged by size:
3925 3261 1984 1162 922 901 794 499
275 268 60 20
ClaI AT'CG_AT
Cuts at: 1554 6305 1554
Size: 4751 9320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 14036
DraI TTT'AAA
Cuts at: 7500 11375 7500
Size: 3875 10196
DraIII CAC_nnn'GTG
Cuts at: 2331 2529 4535 2331
Size: 198 2006 11867
Fragments arranged by size:
11867 2006 198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2810 13078 809
Size: 507 1494 10268 1802
Fragments arranged by size:
10268 1802 1494 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 3652 5893
Size: 54 1425 50 275 18 245 1577 2241
Cuts at: 5893 6223 10537 11518 12407 14036 8
Size: 330 4314 981 889 1629 43
Fragments arranged by size:
4314 2241 1629 1577 1425 981 889 330
275 245 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2075 11518 1537
Size: 275 263 9443 4090
Fragments arranged by size:
9443 4090 275 263
EarI CTCTTCn'nnn_
Cuts at: 1353 3834 4043 4938 9135 9862 12001 1353
Size: 2481 209 895 4197 727 2139 3423
Fragments arranged by size:
4197 3423 2481 2139 895 727 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 12658 13032 13178 387
Size: 1064 138 11069 374 146 1280
Fragments arranged by size:
11069 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4107 4702 8176 9181 9619 11119 4107
Size: 595 3474 1005 438 1500 7059
Fragments arranged by size:
7059 3474 1500 1005 595 438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2676 3013 3075 3181 3775 3851 3963
Size: 393 1044 337 62 106 594 76 112
Cuts at: 3963 4081 4335 4428 4599 4992 5553 5588 6105
Size: 118 254 93 171 393 561 35 517
Cuts at: 6105 6585 6675 6791 7139 7245 7901 7912 8219
Size: 480 90 116 348 106 656 11 307
Cuts at: 8219 8436 8661 8784 9491 9593 10158 10462 10768
Size: 217 225 123 707 102 565 304 306
Cuts at: 10768 10947 11297 11327 12134 12731 13518 13786 1239
Size: 179 350 30 807 597 787 268 1524
Fragments arranged by size:
1524 1044 807 787 707 656 597 594
565 561 517 480 393 393 350 348
337 307 306 304 268 254 225 217
179 171 123 118 116 112 106 106
102 93 90 76 62 35 30 11
EcoNI CCTnn'n_nnAGG
Cuts at: 4395 6630 7739 10044 4395
Size: 2235 1109 2305 8422
Fragments arranged by size:
8422 2305 2235 1109
EcoO109I rG'GnC_Cy
Cuts at: 2023 2189 4866 6886 7366 7877 10756 11326 2023
Size: 166 2677 2020 480 511 2879 570 4768
Fragments arranged by size:
4768 2879 2677 2020 570 511 480 166
EcoRI G'AATT_C
Cuts at: 7043 9291 10581 10904 11380 11758 7043
Size: 2248 1290 323 476 378 9356
Fragments arranged by size:
9356 2248 1290 476 378 323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7931 10601 7931
Size: 2670 11401
FspI TGC'GCA
Cuts at: 12577 12577
Size: 14071
HaeII r_GCGC'y
Cuts at: 1678 13218 1678
Size: 11540 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2764 2796 3117 3149 3349
Size: 32 135 32 1192 32 321 32 200
Cuts at: 3349 3381 3674 3706 4497 4529 4634 4666 5668
Size: 32 293 32 791 32 105 32 1002
Cuts at: 5668 5700 5833 5865 7183 7215 7619 7651 7658
Size: 32 133 32 1318 32 404 32 7
Cuts at: 7658 7690 8621 8653 8782 8814 8976 9008 9203
Size: 32 931 32 129 32 162 32 195
Cuts at: 9203 9235 10234 10266 10933 10965 11250 11282 12712
Size: 32 999 32 667 32 285 32 1430
Cuts at: 12712 12744 12786 12818 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1430 1318 1192 1002 999 931 791
667 404 321 293 285 200 195 162
135 133 129 105 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3810 4114 4240 4418 6323 8521
Size: 988 281 1872 304 126 178 1905 2198
Cuts at: 8521 11415 11661 669
Size: 2894 246 3079
Fragments arranged by size:
3079 2894 2198 1905 1872 988 304 281
246 178 126
HindIII A'AGCT_T
Cuts at: 748 7658 7782 7924 9980 748
Size: 6910 124 142 2056 4839
Fragments arranged by size:
6910 4839 2056 142 124
HpaI GTT'AAC
Cuts at: 4114 4240 11661 4114
Size: 126 7421 6524
Fragments arranged by size:
7421 6524 126
KpnI G_GTAC'C
Cuts at: 8695 9079 11396 8695
Size: 384 2317 11370
Fragments arranged by size:
11370 2317 384
MfeI C'AATT_G
Cuts at: 3037 3258 5897 9315 9633 10329 10368 11670 3037
Size: 221 2639 3418 318 696 39 1302 5438
Fragments arranged by size:
5438 3418 2639 1302 696 318 221 39
MluI A'CGCG_T
Cuts at: 13947 13947
Size: 14071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2833 8614 8626 10356 10629 11182 13185 13369
Size: 1528 5781 12 1730 273 553 2003 184
Cuts at: 13369 1305
Size: 2007
Fragments arranged by size:
5781 2007 2003 1730 1528 553 273 184
12
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5895 10539 10
Size: 54 1425 343 4063 4644 3542
Fragments arranged by size:
4644 4063 3542 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3662 3954 4276 4344 4600 6265 6307
Size: 648 2496 292 322 68 256 1665 42
Cuts at: 6307 6693 7989 9095 9173 9258 11064 12029 12388
Size: 386 1296 1106 78 85 1806 965 359
Cuts at: 12388 12547 518
Size: 159 2042
Fragments arranged by size:
2496 2042 1806 1665 1296 1106 965 648
386 359 322 292 256 159 85 78
68 42
MspA1I CmG'CkG
Cuts at: 1606 2074 2089 4127 4163 4638 4998 5499 6125
Size: 468 15 2038 36 475 360 501 626
Cuts at: 6125 6483 6808 6883 6907 8096 8534 10178 10456
Size: 358 325 75 24 1189 438 1644 278
Cuts at: 10456 11177 11487 12164 13312 13557 1606
Size: 721 310 677 1148 245 2120
Fragments arranged by size:
2120 2038 1644 1189 1148 721 677 626
501 475 468 438 360 358 325 310
278 245 75 36 24 15
NaeI GCC'GGC
Cuts at: 3765 5450 3765
Size: 1685 12386
NcoI C'CATG_G
Cuts at: 513 1065 10083 513
Size: 552 9018 4501
Fragments arranged by size:
9018 4501 552
NdeI CA'TA_TG
Cuts at: 387 2575 3826 5874 10055 387
Size: 2188 1251 2048 4181 4403
Fragments arranged by size:
4403 4181 2188 2048 1251
NgoMIV G'CCGG_C
Cuts at: 3763 5448 3763
Size: 1685 12386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 14071
NotI GC'GGCC_GC
Cuts at: 2075 11518 2075
Size: 9443 4628
NsiI A_TGCA'T
Cuts at: 5096 9052 5096
Size: 3956 10115
NspI r_CATG'y
Cuts at: 1132 1229 2178 2222 2946 3036 3183 3302 6186
Size: 97 949 44 724 90 147 119 2884
Cuts at: 6186 6698 9943 10806 11429 12974 1132
Size: 512 3245 863 623 1545 2229
Fragments arranged by size:
3245 2884 2229 1545 949 863 724 623
512 147 119 97 90 44
PasI CC'CwG_GG
Cuts at: 1348 2225 5336 6154 6244 7036 1348
Size: 877 3111 818 90 792 8383
Fragments arranged by size:
8383 3111 877 818 792 90
PciI A'CATG_T
Cuts at: 2174 2218 2942 3179 10802 12970 2174
Size: 44 724 237 7623 2168 3275
Fragments arranged by size:
7623 3275 2168 724 237 44
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5084 5148 9072 1108
Size: 3976 64 3924 6107
Fragments arranged by size:
6107 3976 3924 64
PfoI T'CCnGG_A
Cuts at: 2971 7320 10638 2971
Size: 4349 3318 6404
Fragments arranged by size:
6404 4349 3318
PmeI GTTT'AAAC
Cuts at: 11375 11375
Size: 14071
PmlI CAC'GTG
Cuts at: 6645 6645
Size: 14071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3849 3881 4815 4847 12140 12172 13653 13685 3849
Size: 32 934 32 7293 32 1481 32 4235
Fragments arranged by size:
7293 4235 1481 934 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2023 2189 4866 7366 7877 2023
Size: 166 2677 2500 511 8217
Fragments arranged by size:
8217 2677 2500 511 166
PshAI GACnn'nnGTC
Cuts at: 1613 3495 4482 10125 1613
Size: 1882 987 5643 5559
Fragments arranged by size:
5643 5559 1882 987
PsiI TTA'TAA
Cuts at: 11641 11641
Size: 14071
PspOMI G'GGCC_C
Cuts at: 6885 6885
Size: 14071
PspXI vC'TCGA_Gb
Cuts at: 5958 5958
Size: 14071
PstI C_TGCA'G
Cuts at: 830 3419 5192 5495 5613 7246 7774 10264 11423
Size: 2589 1773 303 118 1633 528 2490 1159
Cuts at: 11423 830
Size: 3478
Fragments arranged by size:
3478 2589 2490 1773 1633 1159 528 303
118
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 14071
PvuII CAG'CTG
Cuts at: 4163 4638 5499 6125 6907 8096 8534 10178 10456
Size: 475 861 626 782 1189 438 1644 278
Cuts at: 10456 11177 4163
Size: 721 7057
Fragments arranged by size:
7057 1644 1189 861 782 721 626 475
438 278
SacI G_AGCT'C
Cuts at: 4109 4704 8178 9183 9621 11121 4109
Size: 595 3474 1005 438 1500 7059
Fragments arranged by size:
7059 3474 1500 1005 595 438
SalI G'TCGA_C
Cuts at: 1936 11413 1936
Size: 9477 4594
SapI GCTCTTCn'nnn_
Cuts at: 9135 9135
Size: 14071
SbfI CC_TGCA'GG
Cuts at: 11423 11423
Size: 14071
ScaI AGT'ACT
Cuts at: 6518 12319 14031 6518
Size: 5801 1712 6558
Fragments arranged by size:
6558 5801 1712
SexAI A'CCwGG_T
Cuts at: 4675 6217 11136 4675
Size: 1542 4919 7610
Fragments arranged by size:
7610 4919 1542
SfcI C'TryA_G
Cuts at: 826 1045 3415 3493 4164 5188 5383 5491 5609
Size: 219 2370 78 671 1024 195 108 118
Cuts at: 5609 7242 7770 8592 8940 10260 10714 11419 12554
Size: 1633 528 822 348 1320 454 705 1135
Cuts at: 12554 13235 13426 826
Size: 681 191 1471
Fragments arranged by size:
2370 1633 1471 1320 1135 1024 822 705
681 671 528 454 348 219 195 191
118 108 78
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 14071
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 14071
SmaI CCC'GGG
Cuts at: 1872 7064 7289 11398 1872
Size: 5192 225 4109 4545
Fragments arranged by size:
5192 4545 4109 225
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3898 5762 5820 5958 7119 8041
Size: 197 926 1955 1864 58 138 1161 922
Cuts at: 8041 8360 9439 11222 12175 13076 13338 820
Size: 319 1079 1783 953 901 262 1553
Fragments arranged by size:
1955 1864 1783 1553 1161 1079 953 926
922 901 319 262 197 138 58
SnaBI TAC'GTA
Cuts at: 493 11862 493
Size: 11369 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 14071
SphI G_CATG'C
Cuts at: 1132 3036 6186 6698 11429 1132
Size: 1904 3150 512 4731 3774
Fragments arranged by size:
4731 3774 3150 1904 512
SspI AAT'ATT
Cuts at: 5 52 2548 5942 7795 8512 9479 10033 11995
Size: 47 2496 3394 1853 717 967 554 1962
Cuts at: 11995 5
Size: 2081
Fragments arranged by size:
3394 2496 2081 1962 1853 967 717 554
47
StuI AGG'CCT
Cuts at: 1517 2052 7130 9755 10099 1517
Size: 535 5078 2625 344 5489
Fragments arranged by size:
5489 5078 2625 535 344
StyI C'CwwG_G
Cuts at: 513 1065 3227 4223 5205 5625 9725 10083 11322
Size: 552 2162 996 982 420 4100 358 1239
Cuts at: 11322 11510 513
Size: 188 3074
Fragments arranged by size:
4100 3074 2162 1239 996 982 552 420
358 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2824 3951 5155 5548 6953 10520 12098
Size: 1255 1244 1127 1204 393 1405 3567 1578
Cuts at: 12098 12115 12268 12453 12898 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3567 1578 1498 1405 1255 1244 1204 1127
445 393 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 3117 3182
Size: 274 80 33 51 1443 222 918 65
Cuts at: 3182 5273 6516 8155 12317 14029 96
Size: 2091 1243 1639 4162 1712 138
Fragments arranged by size:
4162 2091 1712 1639 1443 1243 918 274
222 138 80 65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2659 2691 3988 4020 4425 4457 4975 5007 6241
Size: 32 1297 32 405 32 518 32 1234
Cuts at: 6241 6273 6478 6510 8980 9012 10860 10892 14031
Size: 32 205 32 2470 32 1848 32 3139
Cuts at: 14031 14063 2659
Size: 32 2667
Fragments arranged by size:
3139 2667 2470 1848 1297 1234 518 405
205 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6626 8228 1316
Size: 5310 1602 7159
Fragments arranged by size:
7159 5310 1602
XbaI T'CTAG_A
Cuts at: 7201 8815 11407 7201
Size: 1614 2592 9865
Fragments arranged by size:
9865 2592 1614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3158 4802 5590 8189 3158
Size: 1644 788 2599 9040
Fragments arranged by size:
9040 2599 1644 788
XhoI C'TCGA_G
Cuts at: 1943 5958 7119 1943
Size: 4015 1161 8895
Fragments arranged by size:
8895 4015 1161
XmaI C'CCGG_G
Cuts at: 1870 7062 7287 11396 1870
Size: 5192 225 4109 4545
Fragments arranged by size:
5192 4545 4109 225
XmnI GAAnn'nnTTC
Cuts at: 3861 7152 7251 7925 8731 9115 9229 9275 10581
Size: 3291 99 674 806 384 114 46 1306
Cuts at: 10581 12200 3861
Size: 1619 5732
Fragments arranged by size:
5732 3291 1619 1306 806 674 384 114
99 46
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4309 8793 11879 276
Size: 53 83 186 936 2775 4484 3086 2468
Fragments arranged by size:
4484 3086 2775 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BsiWI EcoRV FseI FspAI
KasI NarI NruI PacI PsrI RsrII SacII SanDI
SfiI SfoI SrfI SwaI