Restriction map for
pFN21AE2528
(Circular) (Six-base) MAPSORT of: pFN21AE2528.seq Check: 8989 from: 1 to: 6052
pFN21AE2528 6052 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2720 2845 2720
Size: 125 5927
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3342 3862 278
Size: 53 83 186 936 1806 520 2468
Fragments arranged by size:
2468 1806 936 520 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3124 3395 1937
Size: 1187 271 4594
Fragments arranged by size:
4594 1187 271
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 2103 4115 5499 5767 1632
Size: 409 62 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 409 268 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 2540 3297 4951 5928 1203
Size: 1337 757 1654 977 1327
Fragments arranged by size:
1654 1337 1327 977 757
AgeI A'CCGG_T
Cuts at: 4883 4883
Size: 6052
AhdI GACnn_n'nnGTC
Cuts at: 4781 4781
Size: 6052
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 4354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6052
AlwNI CAG_nnn'CTG
Cuts at: 3795 5367 3795
Size: 1572 4480
ApaI G_GGCC'C
Cuts at: 2407 3116 2407
Size: 709 5343
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 2518 2705 3361 3587 3739 5848 1493
Size: 1025 187 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 656 226 187 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2898 3377 1870
Size: 73 955 479 4545
Fragments arranged by size:
4545 955 479 73
AvrII C'CTAG_G
Cuts at: 2851 2851
Size: 6052
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3155 3373 4828 618
Size: 325 217 627 1368 218 1455 1842
Fragments arranged by size:
1842 1455 1368 627 325 218 217
BanII G_rGCy'C
Cuts at: 1391 2407 2891 3116 3371 1391
Size: 1016 484 225 255 4072
Fragments arranged by size:
4072 1016 484 255 225
BbeI G_GCGC'C
Cuts at: 3159 3159
Size: 6052
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5081
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4663 136
Size: 107 122 71 631 605 2991 1525
Fragments arranged by size:
2991 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6047 6047
Size: 6052
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2407 3116 4113 5269 1790
Size: 617 709 997 1156 2573
Fragments arranged by size:
2573 1156 997 709 617
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 2638 3783
Size: 416 53 83 399 24 772 449 1145
Cuts at: 3783 4741 442
Size: 958 1753
Fragments arranged by size:
1753 1145 958 772 449 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2756 3096 4712 1239
Size: 1517 340 1616 2579
Fragments arranged by size:
2579 1616 1517 340
Bpu10I CC'TnA_GC
Cuts at: 2441 2441
Size: 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2692 4177 5042 5340 2692
Size: 1485 865 298 3404
Fragments arranged by size:
3404 1485 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 4715 882
Size: 617 537 2679 2219
Fragments arranged by size:
2679 2219 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3156 3339 3859 4241
Size: 53 83 186 936 1623 183 520 382
Cuts at: 4241 4876 275
Size: 635 1451
Fragments arranged by size:
1623 1451 936 635 520 382 186 183
83 53
BsaWI w'CCGG_w
Cuts at: 2153 4485 4883 5157 5304 5715 2153
Size: 2332 398 274 147 411 2490
Fragments arranged by size:
2490 2332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5827
Fragments arranged by size:
5827 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3224 1186
Size: 2038 4014
BseYI C'CCAG_C
Cuts at: 2777 3115 5255 5751 2777
Size: 338 2140 496 3078
Fragments arranged by size:
3078 2140 496 338
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 2665 2817 3174 1163
Size: 573 900 29 152 357 4041
Fragments arranged by size:
4041 900 573 357 152 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2871 2891 4113 4198 5269 2871
Size: 20 1222 85 1071 3654
Fragments arranged by size:
3654 1222 1071 85 20
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2407 2871 2891 3116 3371 4113 4198
Size: 399 617 464 20 225 255 742 85
Cuts at: 4198 5269 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 617 464 399 255 225
85 20
BspEI T'CCGG_A
Cuts at: 2153 5715 2153
Size: 3562 2490
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2608 2720 2845 3407 844
Size: 1764 112 125 562 3489
Fragments arranged by size:
3489 1764 562 125 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2740 3939 1394
Size: 429 917 1199 3507
Fragments arranged by size:
3507 1199 917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2238 2294 4547 4721 65
Size: 1183 990 56 2253 174 1396
Fragments arranged by size:
2253 1396 1183 990 174 56
BsrFI r'CCGG_y
Cuts at: 1700 1810 4696 4883 1700
Size: 110 2886 187 2869
Fragments arranged by size:
2886 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 4141
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 2841 4112 5124 2841
Size: 1271 1012 3769
Fragments arranged by size:
3769 1271 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6052
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2445 2805 3382 3749 4134 4151 5603 5689
Size: 473 360 577 367 385 17 1452 86
Cuts at: 5689 5701 6047 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 577 473 385 367 360 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2253 2505 2526 3276 513
Size: 552 1188 252 21 750 3289
Fragments arranged by size:
3289 1188 750 552 252 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 3568 4362 4382 3568
Size: 794 20 5238
Fragments arranged by size:
5238 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 2185 3356 2185
Size: 1171 4881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2264 5059 809
Size: 507 948 2795 1802
Fragments arranged by size:
2795 1802 948 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3499 4388 6017
Size: 54 1425 50 275 18 1669 889 1629
Cuts at: 6017 8
Size: 43
Fragments arranged by size:
1669 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2655 3230 3982 1353
Size: 675 627 575 752 3423
Fragments arranged by size:
3423 752 675 627 575
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4639 5013 5159 387
Size: 1064 138 3050 374 146 1280
Fragments arranged by size:
3050 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2889 2889
Size: 6052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2103 2756 3096 4115 4712 5499
Size: 393 409 62 653 340 1019 597 787
Cuts at: 5499 5767 1239
Size: 268 1524
Fragments arranged by size:
1524 1019 787 653 597 409 393 340
268 62
EcoO109I rG'GnC_Cy
Cuts at: 2189 2403 2189
Size: 214 5838
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 3159 5199 1678
Size: 1481 2040 2531
Fragments arranged by size:
2531 2040 1481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 3242 3274 3324
Size: 32 135 32 1556 32 82 32 50
Cuts at: 3324 3356 4693 4725 4767 4799 1373
Size: 32 1337 32 42 32 2626
Fragments arranged by size:
2626 1556 1337 135 82 50 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2269 3001 3396 3642 669
Size: 988 281 331 732 395 246 3079
Fragments arranged by size:
3079 988 732 395 331 281 246
HindIII A'AGCT_T
Cuts at: 748 2560 748
Size: 1812 4240
HpaI GTT'AAC
Cuts at: 3001 3642 3001
Size: 641 5411
KasI G'GCGC_C
Cuts at: 3155 3155
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5166 5350 1305
Size: 3861 184 2007
Fragments arranged by size:
3861 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4230
Fragments arranged by size:
4230 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2412 2864 2945 4010 4369 4528 518
Size: 648 1246 452 81 1065 359 159 2042
Fragments arranged by size:
2042 1246 1065 648 452 359 159 81
MspA1I CmG'CkG
Cuts at: 1606 3468 4145 5293 5538 1606
Size: 1862 677 1148 245 2120
Fragments arranged by size:
2120 1862 1148 677 245
NarI GG'CG_CC
Cuts at: 3156 3156
Size: 6052
NcoI C'CATG_G
Cuts at: 513 1065 2253 2505 2526 3276 513
Size: 552 1188 252 21 750 3289
Fragments arranged by size:
3289 1188 750 552 252 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 2544 3301 3410 4955 1132
Size: 97 1315 757 109 1545 2229
Fragments arranged by size:
2229 1545 1315 757 109 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 2540 3297 4951 2540
Size: 757 1654 3641
Fragments arranged by size:
3641 1654 757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6052
PfoI T'CCnGG_A
Cuts at: 2127 2450 2127
Size: 323 5729
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 4121 4153 5634 5666 2711
Size: 32 1378 32 1481 32 3097
Fragments arranged by size:
3097 1481 1378 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2189 2189
Size: 6052
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PspOMI G'GGCC_C
Cuts at: 2403 3112 2403
Size: 709 5343
PstI C_TGCA'G
Cuts at: 830 3404 830
Size: 2574 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
SacI G_AGCT'C
Cuts at: 2891 2891
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 3394 1936
Size: 1458 4594
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SexAI A'CCwGG_T
Cuts at: 3348 3348
Size: 6052
SfcI C'TryA_G
Cuts at: 826 1045 2260 2470 3125 3400 4535 5216 5407
Size: 219 1215 210 655 275 1135 681 191
Cuts at: 5407 826
Size: 1471
Fragments arranged by size:
1471 1215 1135 681 655 275 219 210
191
SfoI GGC'GCC
Cuts at: 3157 3157
Size: 6052
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 3379 1872
Size: 1507 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2671 2898 4156 5057 5319 820
Size: 197 926 728 227 1258 901 262 1553
Fragments arranged by size:
1553 1258 926 901 728 262 227 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6052
StyI C'CwwG_G
Cuts at: 513 1065 2253 2505 2526 2851 2913 3132 3276
Size: 552 1188 252 21 325 62 219 144
Cuts at: 3276 3491 513
Size: 215 3074
Fragments arranged by size:
3074 1188 552 325 252 219 215 144
62 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2116 2897 4079 4096 4249 4434 4879
Size: 1255 536 781 1182 17 153 185 445
Cuts at: 4879 325
Size: 1498
Fragments arranged by size:
1498 1255 1182 781 536 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 4298 6010
Size: 274 80 33 51 1443 30 2291 1712
Cuts at: 6010 96
Size: 138
Fragments arranged by size:
2291 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6012 6044 6012
Size: 32 6020
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 2898 1943
Size: 955 5097
XmaI C'CCGG_G
Cuts at: 1870 3377 1870
Size: 1507 4545
XmnI GAAnn'nnTTC
Cuts at: 2519 4181 2519
Size: 1662 4390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3340 3860 276
Size: 53 83 186 936 1806 520 2468
Fragments arranged by size:
2468 1806 936 520 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SrfI SwaI XcmI