Restriction map for
pFN21AE2527
(Circular) (Six-base) MAPSORT of: pFN21AE2527.seq Check: 7606 from: 1 to: 10315
pFN21AE2527 10315 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6818 6818
Size: 10315
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6106 8125 278
Size: 53 83 186 936 4570 2019 2468
Fragments arranged by size:
4570 2468 2019 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2740 7000 7489 7658 1937
Size: 803 4260 489 169 4594
Fragments arranged by size:
4594 4260 803 489 169
Acc65I G'GTAC_C
Cuts at: 7636 7636
Size: 10315
AclI AA'CG_TT
Cuts at: 1563 2075 4712 8442 8815 1563
Size: 512 2637 3730 373 3063
Fragments arranged by size:
3730 3063 2637 512 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 3551 3906 5228 5243 5306 5499 5573
Size: 1038 881 355 1322 15 63 193 74
Cuts at: 5573 5805 6281 6315 6628 7305 8378 9762 10030
Size: 232 476 34 313 677 1073 1384 268
Cuts at: 10030 1632
Size: 1917
Fragments arranged by size:
1917 1384 1322 1073 1038 881 677 476
355 313 268 232 193 74 63 34
15
AflII C'TTAA_G
Cuts at: 820 1017 3712 4214 4895 820
Size: 197 2695 502 681 6240
Fragments arranged by size:
6240 2695 681 502 197
AflIII A'CryG_T
Cuts at: 1203 9214 10191 1203
Size: 8011 977 1327
Fragments arranged by size:
8011 1327 977
AgeI A'CCGG_T
Cuts at: 9146 9146
Size: 10315
AhdI GACnn_n'nnGTC
Cuts at: 9044 9044
Size: 10315
AleI CACnn'nnGTG
Cuts at: 1166 6867 1166
Size: 5701 4614
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10315
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6723 6755 6723
Size: 32 10283
AlwNI CAG_nnn'CTG
Cuts at: 5711 7523 8058 9630 5711
Size: 1812 535 1572 6396
Fragments arranged by size:
6396 1812 1572 535
ApaLI G'TGCA_C
Cuts at: 8372 9528 8372
Size: 1156 9159
ApoI r'AATT_y
Cuts at: 1493 2016 2264 2423 2436 2893 3023 3580 3646
Size: 523 248 159 13 457 130 557 66
Cuts at: 3646 3774 3824 3997 4090 4585 5332 5809 5864
Size: 128 50 173 93 495 747 477 55
Cuts at: 5864 5956 5991 6146 6532 6875 7579 7624 7850
Size: 92 35 155 386 343 704 45 226
Cuts at: 7850 8002 10111 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 747 704 557 523 495 477
457 386 343 248 226 173 159 155
152 130 128 93 92 66 55 50
45 35 13
AseI AT'TA_AT
Cuts at: 160 1032 2169 3227 5144 6002 6231 8869 160
Size: 872 1137 1058 1917 858 229 2638 1606
Fragments arranged by size:
2638 1917 1606 1137 1058 872 858 229
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10315
AvaI C'yCGr_G
Cuts at: 1870 1943 7640 1870
Size: 73 5697 4545
Fragments arranged by size:
5697 4545 73
BamHI G'GATC_C
Cuts at: 7645 8012 7645
Size: 367 9948
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7636 9091 618
Size: 325 217 627 5849 1455 1842
Fragments arranged by size:
5849 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4233 5060 7634 1391
Size: 2842 827 2574 4072
Fragments arranged by size:
4072 2842 2574 827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3063 3102 3557 3699 4760 5085 5391
Size: 971 1164 39 455 142 1061 325 306
Cuts at: 5391 5448 5604 5813 6696 928
Size: 57 156 209 883 4547
Fragments arranged by size:
4547 1164 1061 971 883 455 325 306
209 156 142 57 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8506 8540 1903
Size: 34 6569 34 3678
Fragments arranged by size:
6569 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8207 9417 8207
Size: 1210 9105
BclI T'GATC_A
Cuts at: 1548 2295 5469 1548
Size: 747 3174 6394
Fragments arranged by size:
6394 3174 747
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8926 136
Size: 107 122 71 631 605 7254 1525
Fragments arranged by size:
7254 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10310 10310
Size: 10315
BlpI GC'TnA_GC
Cuts at: 3612 3812 4167 5939 7732 3612
Size: 200 355 1772 1793 6195
Fragments arranged by size:
6195 1793 1772 355 200
Bme1580I G_kGCm'C
Cuts at: 1790 8376 9532 1790
Size: 6586 1156 2573
Fragments arranged by size:
6586 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10315
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6956 8046 9004
Size: 416 53 83 399 24 5539 1090 958
Cuts at: 9004 442
Size: 1753
Fragments arranged by size:
5539 1753 1090 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5079 5079
Size: 10315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2700 6265 6953 7013 8975 1239
Size: 815 646 3565 688 60 1962 2579
Fragments arranged by size:
3565 2579 1962 815 688 646 60
Bpu10I CC'TnA_GC
Cuts at: 2792 2792
Size: 10315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2867 4820 5237 5594 7087 7317 8440 9305 9603
Size: 1953 417 357 1493 230 1123 865 298
Cuts at: 9603 2867
Size: 3579
Fragments arranged by size:
3579 1953 1493 1123 865 417 357 298
230
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2669 5583 7258 8978 882
Size: 617 1170 2914 1675 1720 2219
Fragments arranged by size:
2914 2219 1720 1675 1170 617
BsaAI yAC'GTr
Cuts at: 493 1204 4727 8106 493
Size: 711 3523 3379 2702
Fragments arranged by size:
3523 3379 2702 711
BsaBI GATnn'nnATC
Cuts at: 6231 10309 6231
Size: 4078 6237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6103 8122 8504 9139
Size: 53 83 186 936 4570 2019 382 635
Cuts at: 9139 275
Size: 1451
Fragments arranged by size:
4570 2019 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 8748 9146 9420 9567 9978 8748
Size: 398 274 147 411 9085
Fragments arranged by size:
9085 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 10090
Fragments arranged by size:
10090 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 5052 6153 1186
Size: 3866 1101 5348
Fragments arranged by size:
5348 3866 1101
BseYI C'CCAG_C
Cuts at: 3294 9518 10014 3294
Size: 6224 496 3595
Fragments arranged by size:
6224 3595 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2418 2679 3756 6509 6852 1163
Size: 573 682 261 1077 2753 343 4626
Fragments arranged by size:
4626 2753 1077 682 573 343 261
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7765 8526 9554 1540
Size: 275 186 5764 761 1028 2301
Fragments arranged by size:
5764 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3121 4233 5060 6969 8376 8461 9532 3121
Size: 1112 827 1909 1407 85 1071 3904
Fragments arranged by size:
3904 1909 1407 1112 1071 827 85
BsmI GAATG_Cn'
Cuts at: 2235 5317 5380 7826 7919 2235
Size: 3082 63 2446 93 4631
Fragments arranged by size:
4631 3082 2446 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3121 4233 5060 6969 7634 8376 8461
Size: 399 1331 1112 827 1909 665 742 85
Cuts at: 8461 9532 1391
Size: 1071 2174
Fragments arranged by size:
2174 1909 1331 1112 1071 827 742 665
399 85
BspEI T'CCGG_A
Cuts at: 9978 9978
Size: 10315
BspHI T'CATG_A
Cuts at: 2043 2298 3178 3781 4792 5364 5479 5500 5698
Size: 255 880 603 1011 572 115 21 198
Cuts at: 5698 6889 8204 9934 2043
Size: 1191 1315 1730 2424
Fragments arranged by size:
2424 1730 1315 1191 1011 880 603 572
255 198 115 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2529 6818 7581 7670 844
Size: 1685 4289 763 89 3489
Fragments arranged by size:
4289 3489 1685 763 89
BsrBI CCG'CTC
Cuts at: 1394 1823 2656 6273 8202 1394
Size: 429 833 3617 1929 3507
Fragments arranged by size:
3617 3507 1929 833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2531 2585 3475 4388 8810 8984 65
Size: 1183 1283 54 890 913 4422 174 1396
Fragments arranged by size:
4422 1396 1283 1183 913 890 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 8959 9146 1700
Size: 110 7149 187 2869
Fragments arranged by size:
7149 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10315
BssSI C'ACGA_G
Cuts at: 3114 7410 8375 9387 3114
Size: 4296 965 1012 4042
Fragments arranged by size:
4296 4042 1012 965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2471 794
Size: 1677 8638
BstBI TT'CG_AA
Cuts at: 9119 9119
Size: 10315
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10315
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 6658 6869 2056
Size: 4602 211 5502
Fragments arranged by size:
5502 4602 211
BstYI r'GATC_y
Cuts at: 1972 3592 6049 6647 7645 8012 8397 8414 9866
Size: 1620 2457 598 998 367 385 17 1452
Cuts at: 9866 9952 9964 10310 1972
Size: 86 12 346 1977
Fragments arranged by size:
2457 1977 1620 1452 998 598 385 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 6495 513
Size: 552 939 4491 4333
Fragments arranged by size:
4491 4333 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9576
Fragments arranged by size:
9576 683 56
BtsI GCAGTG_nn'
Cuts at: 5171 6874 6919 7539 7831 8625 8645 5171
Size: 1703 45 620 292 794 20 6841
Fragments arranged by size:
6841 1703 794 620 292 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10280
DraI TTT'AAA
Cuts at: 3957 4051 5775 7619 3957
Size: 94 1724 1844 6653
Fragments arranged by size:
6653 1844 1724 94
DraIII CAC_nnn'GTG
Cuts at: 2708 4727 6707 2708
Size: 2019 1980 6316
Fragments arranged by size:
6316 2019 1980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2545 9322 809
Size: 507 1229 6777 1802
Fragments arranged by size:
6777 1802 1229 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 6358 7194
Size: 54 1425 50 275 18 258 4270 836
Cuts at: 7194 7762 8651 10280 8
Size: 568 889 1629 43
Fragments arranged by size:
4270 1629 1425 889 836 568 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7762 1537
Size: 275 5950 4090
Fragments arranged by size:
5950 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 2692 3429 5304 6117 6637 7156 8245
Size: 1293 46 737 1875 813 520 519 1089
Cuts at: 8245 1353
Size: 3423
Fragments arranged by size:
3423 1875 1293 1089 813 737 520 519
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8902 9276 9422 387
Size: 1064 138 7313 374 146 1280
Fragments arranged by size:
7313 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4231 5058 4231
Size: 827 9488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2670 2700 3551 3906 5228 5243
Size: 393 422 616 30 851 355 1322 15
Cuts at: 5243 5306 5499 5573 5805 6265 6281 6315 6628
Size: 63 193 74 232 460 16 34 313
Cuts at: 6628 6953 7013 7305 8378 8975 9762 10030 1239
Size: 325 60 292 1073 597 787 268 1524
Fragments arranged by size:
1524 1322 1073 851 787 616 597 460
422 393 355 325 313 292 268 232
193 74 63 60 34 30 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 6415 7323 6415
Size: 908 9407
EcoRI G'AATT_C
Cuts at: 2436 4090 7624 8002 2436
Size: 1654 3534 378 4749
Fragments arranged by size:
4749 3534 1654 378
EcoRV GAT'ATC
Cuts at: 3312 3312
Size: 10315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3191 3632 4229 6311 7133 3191
Size: 441 597 2082 822 6373
Fragments arranged by size:
6373 2082 822 597 441
FspI TGC'GCA
Cuts at: 8821 8821
Size: 10315
HaeII r_GCGC'y
Cuts at: 1678 9462 1678
Size: 7784 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 3362 3394 3426
Size: 32 135 32 1325 32 433 32 32
Cuts at: 3426 4366 4398 5435 5461 5467 5493 5510 5542
Size: 940 32 1037 26 6 26 17 32
Cuts at: 5542 5891 5923 6665 6697 6890 6922 6988 7020
Size: 349 32 742 32 193 32 66 32
Cuts at: 7020 7255 7287 8956 8988 9030 9062 1373
Size: 235 32 1669 32 42 32 2626
Fragments arranged by size:
2626 1669 1325 1037 940 742 433 349
235 193 135 66 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 26 26 17 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2741 3375 5768 6628 7001 7659
Size: 988 281 803 634 2393 860 373 658
Cuts at: 7659 7905 669
Size: 246 3079
Fragments arranged by size:
3079 2393 988 860 803 658 634 373
281 246
HindIII A'AGCT_T
Cuts at: 748 2975 5318 7076 748
Size: 2227 2343 1758 3987
Fragments arranged by size:
3987 2343 2227 1758
HpaI GTT'AAC
Cuts at: 7905 7905
Size: 10315
KpnI G_GTAC'C
Cuts at: 7640 7640
Size: 10315
MfeI C'AATT_G
Cuts at: 2291 4502 4976 5894 7914 2291
Size: 2211 474 918 2020 4692
Fragments arranged by size:
4692 2211 2020 918 474
MluI A'CGCG_T
Cuts at: 10191 10191
Size: 10315
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2808 3072 4552 5616 9429 9613 1305
Size: 1503 264 1480 1064 3813 184 2007
Fragments arranged by size:
3813 2007 1503 1480 1064 264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6360 7196 10
Size: 54 1425 343 4528 836 3129
Fragments arranged by size:
4528 3129 1425 836 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2249 4160 6473 6867 6894 8273 8632
Size: 648 1083 1911 2313 394 27 1379 359
Cuts at: 8632 8791 518
Size: 159 2042
Fragments arranged by size:
2313 2042 1911 1379 1083 648 394 359
159 27
MspA1I CmG'CkG
Cuts at: 1606 2468 3721 4441 7520 7731 8408 9556 9801
Size: 862 1253 720 3079 211 677 1148 245
Cuts at: 9801 1606
Size: 2120
Fragments arranged by size:
3079 2120 1253 1148 862 720 677 245
211
NcoI C'CATG_G
Cuts at: 513 1065 2004 6495 513
Size: 552 939 4491 4333
Fragments arranged by size:
4491 4333 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10315
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10315
NotI GC'GGCC_GC
Cuts at: 7762 7762
Size: 10315
NspI r_CATG'y
Cuts at: 1132 1229 7673 9218 1132
Size: 97 6444 1545 2229
Fragments arranged by size:
6444 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3352 7220 1348
Size: 2004 3868 4443
Fragments arranged by size:
4443 3868 2004
PciI A'CATG_T
Cuts at: 9214 9214
Size: 10315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5072 5896 6367 1108
Size: 3964 824 471 5056
Fragments arranged by size:
5056 3964 824 471
PmeI GTTT'AAAC
Cuts at: 7619 7619
Size: 10315
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3135 8384 8416 9897 9929 3103
Size: 32 5249 32 1481 32 3489
Fragments arranged by size:
5249 3489 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 6696 1613
Size: 5083 5232
PsiI TTA'TAA
Cuts at: 3030 3864 4968 7885 3030
Size: 834 1104 2917 5460
Fragments arranged by size:
5460 2917 1104 834
PstI C_TGCA'G
Cuts at: 830 5716 6640 7546 7667 830
Size: 4886 924 906 121 3478
Fragments arranged by size:
4886 3478 924 906 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10315
PvuII CAG'CTG
Cuts at: 4441 7520 4441
Size: 3079 7236
SacI G_AGCT'C
Cuts at: 4233 5060 4233
Size: 827 9488
SalI G'TCGA_C
Cuts at: 1936 2739 6999 7657 1936
Size: 803 4260 658 4594
Fragments arranged by size:
4594 4260 803 658
SapI GCTCTTCn'nnn_
Cuts at: 2646 3429 2646
Size: 783 9532
SbfI CC_TGCA'GG
Cuts at: 7667 7667
Size: 10315
ScaI AGT'ACT
Cuts at: 4849 6526 6935 8563 10275 4849
Size: 1677 409 1628 1712 4889
Fragments arranged by size:
4889 1712 1677 1628 409
SexAI A'CCwGG_T
Cuts at: 2151 6483 7267 2151
Size: 4332 784 5199
Fragments arranged by size:
5199 4332 784
SfcI C'TryA_G
Cuts at: 826 1045 3459 4172 5712 5761 6008 6603 6636
Size: 219 2414 713 1540 49 247 595 33
Cuts at: 6636 7542 7663 8798 9479 9670 826
Size: 906 121 1135 681 191 1471
Fragments arranged by size:
2414 1540 1471 1135 906 713 681 595
247 219 191 121 49 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10315
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10315
SmaI CCC'GGG
Cuts at: 1872 7642 1872
Size: 5770 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 3712 4214 4799 4895 5216
Size: 197 926 903 866 502 585 96 321
Cuts at: 5216 5573 7102 7332 8419 9320 9582 820
Size: 357 1529 230 1087 901 262 1553
Fragments arranged by size:
1553 1529 1087 926 903 901 866 585
502 357 321 262 230 197 96
SnaBI TAC'GTA
Cuts at: 493 8106 493
Size: 7613 2702
SpeI A'CTAG_T
Cuts at: 152 3320 152
Size: 3168 7147
SphI G_CATG'C
Cuts at: 1132 7673 1132
Size: 6541 3774
SspI AAT'ATT
Cuts at: 5 52 8239 5
Size: 47 8187 2081
Fragments arranged by size:
8187 2081 47
StuI AGG'CCT
Cuts at: 1517 3983 6452 1517
Size: 2466 2469 5380
Fragments arranged by size:
5380 2469 2466
StyI C'CwwG_G
Cuts at: 513 1065 2004 6366 6495 7479 7754 513
Size: 552 939 4362 129 984 275 3074
Fragments arranged by size:
4362 3074 984 939 552 275 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6280 6442 8342 8359 8512 8697 9142
Size: 1255 4700 162 1900 17 153 185 445
Cuts at: 9142 325
Size: 1498
Fragments arranged by size:
4700 1900 1498 1255 445 185 162 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4847 6075 6524
Size: 274 80 33 51 1443 2870 1228 449
Cuts at: 6524 6933 7087 7564 8561 10273 96
Size: 409 154 477 997 1712 138
Fragments arranged by size:
2870 1712 1443 1228 997 477 449 409
274 154 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 10275 10307 2225
Size: 32 8018 32 2233
Fragments arranged by size:
8018 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5272 7269 1316
Size: 3956 1997 4362
Fragments arranged by size:
4362 3956 1997
XbaI T'CTAG_A
Cuts at: 7651 7651
Size: 10315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6481 6481
Size: 10315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10315
XmaI C'CCGG_G
Cuts at: 1870 7640 1870
Size: 5770 4545
XmnI GAAnn'nnTTC
Cuts at: 2436 3438 5496 6291 8444 2436
Size: 1002 2058 795 2153 4307
Fragments arranged by size:
4307 2153 2058 1002 795
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6104 8123 276
Size: 53 83 186 936 4570 2019 2468
Fragments arranged by size:
4570 2468 2019 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI