Restriction map for
pFN21AE2526
(Circular) (Six-base) MAPSORT of: pFN21AE2526.seq Check: 6973 from: 1 to: 5281
pFN21AE2526 5281 bp
With 182 enzymes: SgfI *
June 11, 2014 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3091 278
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 1937
Size: 687 4594
Acc65I G'GTAC_C
Cuts at: 2602 2602
Size: 5281
AclI AA'CG_TT
Cuts at: 1563 3408 3781 1563
Size: 1845 373 3063
Fragments arranged by size:
3063 1845 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2467 3344 4728 4996 1632
Size: 835 877 1384 268 1917
Fragments arranged by size:
1917 1384 877 835 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5084
AflIII A'CryG_T
Cuts at: 1203 4180 5157 1203
Size: 2977 977 1327
Fragments arranged by size:
2977 1327 977
AgeI A'CCGG_T
Cuts at: 4112 4112
Size: 5281
AhdI GACnn_n'nnGTC
Cuts at: 4010 4010
Size: 5281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5281
AlwNI CAG_nnn'CTG
Cuts at: 3024 4596 3024
Size: 1572 3709
ApaLI G'TGCA_C
Cuts at: 3338 4494 3338
Size: 1156 4125
ApoI r'AATT_y
Cuts at: 1493 2590 2816 2968 5077 1493
Size: 1097 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3835 160
Size: 872 2803 1606
Fragments arranged by size:
2803 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
AvaI C'yCGr_G
Cuts at: 1870 1943 2353 2532 2606 1870
Size: 73 410 179 74 4545
Fragments arranged by size:
4545 410 179 74 73
BamHI G'GATC_C
Cuts at: 2611 2978 2611
Size: 367 4914
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2276 2327 2602 4057 618
Size: 325 217 627 489 51 275 1455 1842
Fragments arranged by size:
1842 1455 627 489 325 275 217 51
BanII G_rGCy'C
Cuts at: 1391 2342 2553 2600 1391
Size: 951 211 47 4072
Fragments arranged by size:
4072 951 211 47
BbeI G_GCGC'C
Cuts at: 2280 2280
Size: 5281
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2104 2473 928
Size: 971 205 369 3736
Fragments arranged by size:
3736 971 369 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3472 3506 1903
Size: 34 50 34 1451 34 3678
Fragments arranged by size:
3678 1451 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3173 4383 3173
Size: 1210 4071
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3892 136
Size: 107 122 71 631 605 2220 1525
Fragments arranged by size:
2220 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5276 5276
Size: 5281
BlpI GC'TnA_GC
Cuts at: 2698 2698
Size: 5281
Bme1580I G_kGCm'C
Cuts at: 1790 3342 4498 1790
Size: 1552 1156 2573
Fragments arranged by size:
2573 1552 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5281
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3012 3970 442
Size: 416 53 83 399 24 1595 958 1753
Fragments arranged by size:
1753 1595 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3941 1239
Size: 2702 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 3406 4271 4569 2567
Size: 839 865 298 3279
Fragments arranged by size:
3279 865 839 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3944 882
Size: 617 2445 2219
Fragments arranged by size:
2445 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2221 3072 493
Size: 711 1017 851 2702
Fragments arranged by size:
2702 1017 851 711
BsaBI GATnn'nnATC
Cuts at: 5275 5275
Size: 5281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 2439 3088 3470
Size: 53 83 186 936 744 162 649 382
Cuts at: 3470 4105 275
Size: 635 1451
Fragments arranged by size:
1451 936 744 649 635 382 186 162
83 53
BsaWI w'CCGG_w
Cuts at: 3714 4112 4386 4533 4944 3714
Size: 398 274 147 411 4051
Fragments arranged by size:
4051 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 4201
Fragments arranged by size:
4201 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 1186
Size: 1063 4218
BseYI C'CCAG_C
Cuts at: 2092 2153 4484 4980 2092
Size: 61 2331 496 2393
Fragments arranged by size:
2393 2331 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2276 1163
Size: 573 456 84 4168
Fragments arranged by size:
4168 573 456 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2434 2731 3492 4520 1540
Size: 275 186 433 297 761 1028 2301
Fragments arranged by size:
2301 1028 761 433 297 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 3342 3427 4498 2553
Size: 789 85 1071 3336
Fragments arranged by size:
3336 1071 789 85
BsmI GAATG_Cn'
Cuts at: 2263 2792 2885 2263
Size: 529 93 4659
Fragments arranged by size:
4659 529 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2354 2354
Size: 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2342 2553 2600 3342 3427 4498 1391
Size: 399 552 211 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 552 399 211 85 47
BspEI T'CCGG_A
Cuts at: 4944 4944
Size: 5281
BspHI T'CATG_A
Cuts at: 3170 4900 3170
Size: 1730 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2636 844
Size: 1792 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3168 1394
Size: 429 1345 3507
Fragments arranged by size:
3507 1345 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3776 3950 65
Size: 1183 2528 174 1396
Fragments arranged by size:
2528 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2022 3925 4112 1700
Size: 110 212 1903 187 2869
Fragments arranged by size:
2869 1903 212 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5281
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5281
BssSI C'ACGA_G
Cuts at: 2165 3341 4353 2165
Size: 1176 1012 3093
Fragments arranged by size:
3093 1176 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5281
BstBI TT'CG_AA
Cuts at: 4085 4085
Size: 5281
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5281
BstXI CCAn_nnnn'nTGG
Cuts at: 2415 2415
Size: 5281
BstYI r'GATC_y
Cuts at: 1972 2611 2978 3363 3380 4832 4918 4930 5276
Size: 639 367 385 17 1452 86 12 346
Cuts at: 5276 1972
Size: 1977
Fragments arranged by size:
1977 1452 639 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2344 513
Size: 552 951 328 3450
Fragments arranged by size:
3450 951 552 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4542
Fragments arranged by size:
4542 683 56
BtsI GCAGTG_nn'
Cuts at: 2015 2249 2797 3591 3611 2015
Size: 234 548 794 20 3685
Fragments arranged by size:
3685 794 548 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2376 2411 529
Size: 35 1812 35 3399
Fragments arranged by size:
3399 1812 35 35
DraI TTT'AAA
Cuts at: 2585 2585
Size: 5281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4288 809
Size: 507 2972 1802
Fragments arranged by size:
2972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2347 2431 2469
Size: 54 1425 50 275 18 517 84 38
Cuts at: 2469 2569 2728 3617 5246 8
Size: 100 159 889 1629 43
Fragments arranged by size:
1629 1425 889 517 275 159 100 84
54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 2431 2728 1537
Size: 275 619 297 4090
Fragments arranged by size:
4090 619 297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2327 2513 2523 3211 1353
Size: 765 209 186 10 688 3423
Fragments arranged by size:
3423 765 688 209 186 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3868 4242 4388 387
Size: 1064 138 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 2551
Size: 5281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2467 3344 3941 4728 4996 1239
Size: 393 835 877 597 787 268 1524
Fragments arranged by size:
1524 877 835 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2536 2575 2536
Size: 39 5242
EcoRI G'AATT_C
Cuts at: 2590 2968 2590
Size: 378 4903
FspI TGC'GCA
Cuts at: 3787 3787
Size: 5281
HaeII r_GCGC'y
Cuts at: 1678 2280 4428 1678
Size: 602 2148 2531
Fragments arranged by size:
2531 2148 602
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3922 3954 3996 4028 1373
Size: 32 135 32 2350 32 42 32 2626
Fragments arranged by size:
2626 2350 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2625 2871 669
Size: 988 281 687 246 3079
Fragments arranged by size:
3079 988 687 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5281
HpaI GTT'AAC
Cuts at: 2871 2871
Size: 5281
KasI G'GCGC_C
Cuts at: 2276 2276
Size: 5281
KpnI G_GTAC'C
Cuts at: 2606 2606
Size: 5281
MfeI C'AATT_G
Cuts at: 2880 2880
Size: 5281
MluI A'CGCG_T
Cuts at: 5157 5157
Size: 5281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4395 4579 1305
Size: 3090 184 2007
Fragments arranged by size:
3090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2571 10
Size: 54 1425 343 739 2720
Fragments arranged by size:
2720 1425 739 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3239 3598 3757 518
Size: 648 1251 822 359 159 2042
Fragments arranged by size:
2042 1251 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2346 2393 2697 3374 4522 4767 1606
Size: 457 283 47 304 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 457 304 283 245 47
NaeI GCC'GGC
Cuts at: 2024 2024
Size: 5281
NarI GG'CG_CC
Cuts at: 2277 2277
Size: 5281
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4729
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5281
NgoMIV G'CCGG_C
Cuts at: 2022 2022
Size: 5281
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5281
NotI GC'GGCC_GC
Cuts at: 2728 2728
Size: 5281
NspI r_CATG'y
Cuts at: 1132 1229 2639 4184 1132
Size: 97 1410 1545 2229
Fragments arranged by size:
2229 1545 1410 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5281
PciI A'CATG_T
Cuts at: 4180 4180
Size: 5281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2170 1108
Size: 902 160 4219
Fragments arranged by size:
4219 902 160
PmeI GTTT'AAAC
Cuts at: 2585 2585
Size: 5281
PmlI CAC'GTG
Cuts at: 2221 2221
Size: 5281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4863 4895 3350
Size: 32 1481 32 3736
Fragments arranged by size:
3736 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2575 2575
Size: 5281
PshAI GACnn'nnGTC
Cuts at: 1613 2075 1613
Size: 462 4819
PsiI TTA'TAA
Cuts at: 2851 2851
Size: 5281
PstI C_TGCA'G
Cuts at: 830 2244 2633 830
Size: 1414 389 3478
Fragments arranged by size:
3478 1414 389
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5281
PvuII CAG'CTG
Cuts at: 2393 2393
Size: 5281
SacI G_AGCT'C
Cuts at: 2553 2553
Size: 5281
SacII CC_GC'GG
Cuts at: 2347 2347
Size: 5281
SalI G'TCGA_C
Cuts at: 1936 2623 1936
Size: 687 4594
SapI GCTCTTCn'nnn_
Cuts at: 2327 2327
Size: 5281
SbfI CC_TGCA'GG
Cuts at: 2633 2633
Size: 5281
ScaI AGT'ACT
Cuts at: 3529 5241 3529
Size: 1712 3569
SfcI C'TryA_G
Cuts at: 826 1045 2240 2629 3764 4445 4636 826
Size: 219 1195 389 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 681 389 219 191
SfoI GGC'GCC
Cuts at: 2278 2278
Size: 5281
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5281
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5281
SmaI CCC'GGG
Cuts at: 1872 2608 1872
Size: 736 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 3385 4286 4548 820
Size: 197 926 603 839 901 262 1553
Fragments arranged by size:
1553 926 901 839 603 262 197
SnaBI TAC'GTA
Cuts at: 493 3072 493
Size: 2579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5281
SphI G_CATG'C
Cuts at: 1132 2639 1132
Size: 1507 3774
SspI AAT'ATT
Cuts at: 5 52 3205 5
Size: 47 3153 2081
Fragments arranged by size:
3153 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5281
StyI C'CwwG_G
Cuts at: 513 1065 2334 2720 513
Size: 552 1269 386 3074
Fragments arranged by size:
3074 1269 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2226 2236 2504 3308 3325 3478 3663
Size: 1255 646 10 268 804 17 153 185
Cuts at: 3663 4108 325
Size: 445 1498
Fragments arranged by size:
1498 1255 804 646 445 268 185 153
17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2368 3527 5239
Size: 274 80 33 51 1443 391 1159 1712
Cuts at: 5239 96
Size: 138
Fragments arranged by size:
1712 1443 1159 391 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 5241 5273 2228
Size: 32 2981 32 2236
Fragments arranged by size:
2981 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2477 1316
Size: 1161 4120
XbaI T'CTAG_A
Cuts at: 2617 2617
Size: 5281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5281
XmaI C'CCGG_G
Cuts at: 1870 2606 1870
Size: 736 4545
XmnI GAAnn'nnTTC
Cuts at: 3410 3410
Size: 5281
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3089 276
Size: 53 83 186 936 1555 2468
Fragments arranged by size:
2468 1555 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI XcmI