Restriction map for
pFN21AE2520
(Circular) (Six-base) MAPSORT of: pFN21AE2520.seq Check: 5886 from: 1 to: 6478
pFN21AE2520 6478 bp
With 182 enzymes: SgfI *
March 20, 2014 17:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4288 278
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3821 1937
Size: 1884 4594
Acc65I G'GTAC_C
Cuts at: 3799 3799
Size: 6478
AclI AA'CG_TT
Cuts at: 1563 4605 4978 1563
Size: 3042 373 3063
Fragments arranged by size:
3063 3042 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2967 3698 4541 5925 6193 1632
Size: 1335 731 843 1384 268 1917
Fragments arranged by size:
1917 1384 1335 843 731 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6281
AflIII A'CryG_T
Cuts at: 1203 5377 6354 1203
Size: 4174 977 1327
Fragments arranged by size:
4174 1327 977
AgeI A'CCGG_T
Cuts at: 5309 5309
Size: 6478
AhdI GACnn_n'nnGTC
Cuts at: 3583 5207 3583
Size: 1624 4854
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6478
AlwNI CAG_nnn'CTG
Cuts at: 2972 3440 3757 4221 5793 2972
Size: 468 317 464 1572 3657
Fragments arranged by size:
3657 1572 468 464 317
ApaI G_GGCC'C
Cuts at: 3012 3012
Size: 6478
ApaLI G'TGCA_C
Cuts at: 4535 5691 4535
Size: 1156 5322
ApoI r'AATT_y
Cuts at: 1493 2338 2427 3787 4013 4165 6274 1493
Size: 845 89 1360 226 152 2109 1697
Fragments arranged by size:
2109 1697 1360 845 226 152 89
AseI AT'TA_AT
Cuts at: 160 1032 5032 160
Size: 872 4000 1606
Fragments arranged by size:
4000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
AvaI C'yCGr_G
Cuts at: 1870 1943 3803 1870
Size: 73 1860 4545
Fragments arranged by size:
4545 1860 73
BamHI G'GATC_C
Cuts at: 3808 4175 3808
Size: 367 6111
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 3799 5254 618
Size: 325 217 627 287 1725 1455 1842
Fragments arranged by size:
1842 1725 1455 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 3012 3735 3797 1391
Size: 1621 723 62 4072
Fragments arranged by size:
4072 1621 723 62
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2293 2443 3162 928
Size: 971 394 150 719 4244
Fragments arranged by size:
4244 971 719 394 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4669 4703 1903
Size: 34 2732 34 3678
Fragments arranged by size:
3678 2732 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3223 4370 5580 3223
Size: 1147 1210 4121
Fragments arranged by size:
4121 1210 1147
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6478
BfrBI ATG'CAT
Cuts at: 2320 2320
Size: 6478
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3359 5089 136
Size: 107 122 71 631 605 1687 1730 1525
Fragments arranged by size:
1730 1687 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6473 6473
Size: 6478
BlpI GC'TnA_GC
Cuts at: 3895 3895
Size: 6478
Bme1580I G_kGCm'C
Cuts at: 1790 3012 3355 4539 5695 1790
Size: 1222 343 1184 1156 2573
Fragments arranged by size:
2573 1222 1184 1156 343
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6478
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 4209 5167
Size: 416 53 83 399 24 2210 582 958
Cuts at: 5167 442
Size: 1753
Fragments arranged by size:
2210 1753 958 582 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 6478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2507 2567 2597 2636 2654 2672 2729
Size: 1253 15 60 30 39 18 18 57
Cuts at: 2729 3523 5138 1239
Size: 794 1615 2579
Fragments arranged by size:
2579 1615 1253 794 60 57 39 30
18 18 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2376 4603 5468 5766 2376
Size: 2227 865 298 3088
Fragments arranged by size:
3088 2227 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 5141 882
Size: 617 774 2868 2219
Fragments arranged by size:
2868 2219 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 4269 493
Size: 711 3065 2702
Fragments arranged by size:
3065 2702 711
BsaBI GATnn'nnATC
Cuts at: 6472 6472
Size: 6478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4285 4667 5302 275
Size: 53 83 186 936 2752 382 635 1451
Fragments arranged by size:
2752 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4911 5309 5583 5730 6141 4911
Size: 398 274 147 411 5248
Fragments arranged by size:
5248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3028 3058 1178
Size: 30 165 30 1625 30 4598
Fragments arranged by size:
4598 1625 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2820 2940 2988 3012 3048 3057 3081 3084
Size: 1634 120 48 24 36 9 24 3
Cuts at: 3084 3382 1186
Size: 298 4282
Fragments arranged by size:
4282 1634 298 120 48 36 24 24
9 3
BseYI C'CCAG_C
Cuts at: 2845 5681 6177 2845
Size: 2836 496 3146
Fragments arranged by size:
3146 2836 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2958 1163
Size: 573 1222 4683
Fragments arranged by size:
4683 1222 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2898 3928 4689 5717 1540
Size: 275 186 897 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 897 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 4539 4624 5695 3735
Size: 804 85 1071 4518
Fragments arranged by size:
4518 1071 804 85
BsmI GAATG_Cn'
Cuts at: 3259 3989 4082 3259
Size: 730 93 5655
Fragments arranged by size:
5655 730 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3012 3355 3735 3797 4539 4624 5695
Size: 399 1222 343 380 62 742 85 1071
Cuts at: 5695 1391
Size: 2174
Fragments arranged by size:
2174 1222 1071 742 399 380 343 85
62
BspEI T'CCGG_A
Cuts at: 6141 6141
Size: 6478
BspHI T'CATG_A
Cuts at: 4367 6097 4367
Size: 1730 4748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 3833 844
Size: 1551 1438 3489
Fragments arranged by size:
3489 1551 1438
BsrBI CCG'CTC
Cuts at: 1394 1823 2476 2620 2638 2656 4365 1394
Size: 429 653 144 18 18 1709 3507
Fragments arranged by size:
3507 1709 653 429 144 18 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 2360 4973 5147 65
Size: 1183 1112 2613 174 1396
Fragments arranged by size:
2613 1396 1183 1112 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2893 5122 5309 1700
Size: 110 1083 2229 187 2869
Fragments arranged by size:
2869 2229 1083 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6478
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6478
BssSI C'ACGA_G
Cuts at: 4538 5550 4538
Size: 1012 5466
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2036 794
Size: 1242 5236
BstBI TT'CG_AA
Cuts at: 5282 5282
Size: 6478
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6478
BstYI r'GATC_y
Cuts at: 1972 3808 4175 4560 4577 6029 6115 6127 6473
Size: 1836 367 385 17 1452 86 12 346
Cuts at: 6473 1972
Size: 1977
Fragments arranged by size:
1977 1836 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3298 513
Size: 552 2233 3693
Fragments arranged by size:
3693 2233 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5739
Fragments arranged by size:
5739 683 56
BtsI GCAGTG_nn'
Cuts at: 3994 4788 4808 3994
Size: 794 20 5664
Fragments arranged by size:
5664 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6478
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6443
DraI TTT'AAA
Cuts at: 3782 3782
Size: 6478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2174 5485 809
Size: 507 858 3311 1802
Fragments arranged by size:
3311 1802 858 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2895 3360 3925
Size: 54 1425 50 275 18 1065 465 565
Cuts at: 3925 4814 6443 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1065 889 565 465 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2895 3925 1537
Size: 275 1083 1030 4090
Fragments arranged by size:
4090 1083 1030 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2860 3463 3723 4408 1353
Size: 1507 603 260 685 3423
Fragments arranged by size:
3423 1507 685 603 260
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3262 5065 5439 5585 387
Size: 1064 138 1673 1803 374 146 1280
Fragments arranged by size:
1803 1673 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3733 3733
Size: 6478
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2507 2567 2597 2636 2654 2672
Size: 393 860 15 60 30 39 18 18
Cuts at: 2672 2729 2967 3523 3698 4541 5138 5925 6193
Size: 57 238 556 175 843 597 787 268
Cuts at: 6193 1239
Size: 1524
Fragments arranged by size:
1524 860 843 787 597 556 393 268
238 175 60 57 39 30 18 18
15
EcoNI CCTnn'n_nnAGG
Cuts at: 3003 3003
Size: 6478
EcoO109I rG'GnC_Cy
Cuts at: 2969 3008 3009 3585 2969
Size: 39 1 576 5862
Fragments arranged by size:
5862 576 39 1
EcoRI G'AATT_C
Cuts at: 3787 4165 3787
Size: 378 6100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3507 3507
Size: 6478
FspI TGC'GCA
Cuts at: 4984 4984
Size: 6478
HaeII r_GCGC'y
Cuts at: 1678 5625 1678
Size: 3947 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2097 2129 2891
Size: 32 135 32 436 32 57 32 762
Cuts at: 2891 2923 3569 3601 5119 5151 5193 5225 1373
Size: 32 646 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 762 646 436 135 57 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3822 4068 669
Size: 988 281 1884 246 3079
Fragments arranged by size:
3079 1884 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6478
HpaI GTT'AAC
Cuts at: 4068 4068
Size: 6478
KpnI G_GTAC'C
Cuts at: 3803 3803
Size: 6478
MfeI C'AATT_G
Cuts at: 3189 4077 3189
Size: 888 5590
MluI A'CGCG_T
Cuts at: 6354 6354
Size: 6478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 5592 5776 1305
Size: 1191 3096 184 2007
Fragments arranged by size:
3096 2007 1191 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3362 10
Size: 54 1425 343 1530 3126
Fragments arranged by size:
3126 1530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4436 4795 4954 518
Size: 648 3270 359 159 2042
Fragments arranged by size:
3270 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2081 2575 2770 3185 3894 4571 5719 5964
Size: 475 494 195 415 709 677 1148 245
Cuts at: 5964 1606
Size: 2120
Fragments arranged by size:
2120 1148 709 677 494 475 415 245
195
NaeI GCC'GGC
Cuts at: 2895 2895
Size: 6478
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5926
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6478
NgoMIV G'CCGG_C
Cuts at: 2893 2893
Size: 6478
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6478
NotI GC'GGCC_GC
Cuts at: 3925 3925
Size: 6478
NsiI A_TGCA'T
Cuts at: 2322 2322
Size: 6478
NspI r_CATG'y
Cuts at: 1132 1229 2324 3836 5381 1132
Size: 97 1095 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 1095 97
PasI CC'CwG_GG
Cuts at: 1348 3005 1348
Size: 1657 4821
PciI A'CATG_T
Cuts at: 5377 5377
Size: 6478
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2084 1108
Size: 976 5502
PmeI GTTT'AAAC
Cuts at: 3782 3782
Size: 6478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 4547 4579 6060 6092 3260
Size: 32 1255 32 1481 32 3646
Fragments arranged by size:
3646 1481 1255 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3585 3585
Size: 6478
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6478
PsiI TTA'TAA
Cuts at: 4048 4048
Size: 6478
PspOMI G'GGCC_C
Cuts at: 3008 3008
Size: 6478
PstI C_TGCA'G
Cuts at: 830 3778 3830 830
Size: 2948 52 3478
Fragments arranged by size:
3478 2948 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6478
PvuII CAG'CTG
Cuts at: 2081 2575 2770 3185 2081
Size: 494 195 415 5374
Fragments arranged by size:
5374 494 415 195
SacI G_AGCT'C
Cuts at: 3735 3735
Size: 6478
SalI G'TCGA_C
Cuts at: 1936 3820 1936
Size: 1884 4594
SapI GCTCTTCn'nnn_
Cuts at: 3723 3723
Size: 6478
SbfI CC_TGCA'GG
Cuts at: 3830 3830
Size: 6478
ScaI AGT'ACT
Cuts at: 4726 6438 4726
Size: 1712 4766
SfcI C'TryA_G
Cuts at: 826 1045 3774 3826 4961 5642 5833 826
Size: 219 2729 52 1135 681 191 1471
Fragments arranged by size:
2729 1471 1135 681 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6478
SmaI CCC'GGG
Cuts at: 1872 3805 1872
Size: 1933 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2391 4582 5483 5745 820
Size: 197 926 448 2191 901 262 1553
Fragments arranged by size:
2191 1553 926 901 448 262 197
SnaBI TAC'GTA
Cuts at: 493 4269 493
Size: 3776 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6478
SphI G_CATG'C
Cuts at: 1132 2324 3836 1132
Size: 1192 1512 3774
Fragments arranged by size:
3774 1512 1192
SspI AAT'ATT
Cuts at: 5 52 4402 5
Size: 47 4350 2081
Fragments arranged by size:
4350 2081 47
StuI AGG'CCT
Cuts at: 1517 3455 1517
Size: 1938 4540
StyI C'CwwG_G
Cuts at: 513 1065 3491 3554 3917 513
Size: 552 2426 63 363 3074
Fragments arranged by size:
3074 2426 552 363 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 4505 4522 4675 4860 5305 325
Size: 1255 482 2443 17 153 185 445 1498
Fragments arranged by size:
2443 1498 1255 482 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2211 3467 4724
Size: 274 80 33 51 1443 234 1256 1257
Cuts at: 4724 6436 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1257 1256 274 234 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2217 2249 3028 3060 6438 6470 2217
Size: 32 779 32 3378 32 2225
Fragments arranged by size:
3378 2225 779 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6478
XbaI T'CTAG_A
Cuts at: 3814 3814
Size: 6478
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6478
XmaI C'CCGG_G
Cuts at: 1870 3803 1870
Size: 1933 4545
XmnI GAAnn'nnTTC
Cuts at: 4607 4607
Size: 6478
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4286 276
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NarI NruI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI