Restriction map for
pFN21AE2517
(Circular) (Six-base) MAPSORT of: pFN21AE2517.seq Check: 2753 from: 1 to: 6028
pFN21AE2517 6028 bp
With 182 enzymes: SgfI *
February 25, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2024 3371 1937
Size: 87 1347 4594
Fragments arranged by size:
4594 1347 87
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4091 5475 5743 1632
Size: 2459 1384 268 1917
Fragments arranged by size:
2459 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 4927 5904 1203
Size: 3724 977 1327
Fragments arranged by size:
3724 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 2678 1166
Size: 1512 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 2071 3771 5343 2071
Size: 1700 1572 2756
Fragments arranged by size:
2756 1700 1572
ApaLI G'TGCA_C
Cuts at: 4085 5241 4085
Size: 1156 4872
ApoI r'AATT_y
Cuts at: 1493 2594 3337 3563 3715 5824 1493
Size: 1101 743 226 152 2109 1697
Fragments arranged by size:
2109 1697 1101 743 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 3353 1870
Size: 73 1410 4545
Fragments arranged by size:
4545 1410 73
BamHI G'GATC_C
Cuts at: 3358 3725 3358
Size: 367 5661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3349 4804 618
Size: 325 217 627 1562 1455 1842
Fragments arranged by size:
1842 1562 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3347 1391
Size: 1956 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5057
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4639 136
Size: 107 122 71 631 605 2967 1525
Fragments arranged by size:
2967 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6023 6023
Size: 6028
BlpI GC'TnA_GC
Cuts at: 2072 3445 2072
Size: 1373 4655
Bme1580I G_kGCm'C
Cuts at: 1790 2709 4089 5245 1790
Size: 919 1380 1156 2573
Fragments arranged by size:
2573 1380 1156 919
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3033 3759 4717
Size: 416 53 83 399 24 1616 726 958
Cuts at: 4717 442
Size: 1753
Fragments arranged by size:
1753 1616 958 726 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2502 2502
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 4688 1239
Size: 888 2561 2579
Fragments arranged by size:
2579 2561 888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 4153 5018 5316 2252
Size: 1901 865 298 2964
Fragments arranged by size:
2964 1901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4691 882
Size: 617 3192 2219
Fragments arranged by size:
3192 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2419 3819 493
Size: 711 1215 1400 2702
Fragments arranged by size:
2702 1400 1215 711
BsaBI GATnn'nnATC
Cuts at: 2613 2736 2844 6022 2613
Size: 123 108 3178 2619
Fragments arranged by size:
3178 2619 123 108
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3835 4217 4852 275
Size: 53 83 186 936 2302 382 635 1451
Fragments arranged by size:
2302 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2400 4461 4859 5133 5280 5691 2400
Size: 2061 398 274 147 411 2737
Fragments arranged by size:
2737 2061 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5803
Fragments arranged by size:
5803 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6028
BseYI C'CCAG_C
Cuts at: 3312 5231 5727 3312
Size: 1919 496 3613
Fragments arranged by size:
3613 1919 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5455
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 4239 5267 1540
Size: 275 186 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4089 4174 5245 4089
Size: 85 1071 4872
Fragments arranged by size:
4872 1071 85
BsmI GAATG_Cn'
Cuts at: 2824 3273 3539 3632 2824
Size: 449 266 93 5220
Fragments arranged by size:
5220 449 266 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2103 2103
Size: 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2709 3347 4089 4174 5245 1391
Size: 399 919 638 742 85 1071 2174
Fragments arranged by size:
2174 1071 919 742 638 399 85
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3383 844
Size: 2539 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 1394
Size: 429 2092 3507
Fragments arranged by size:
3507 2092 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4523 4697 65
Size: 1183 3275 174 1396
Fragments arranged by size:
3275 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 4672 4859 1700
Size: 110 222 2640 187 2869
Fragments arranged by size:
2869 2640 222 187 110
BsrGI T'GTAC_A
Cuts at: 96 3206 96
Size: 3110 2918
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 4088 5100 4088
Size: 1012 5016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3130 794
Size: 2336 3692
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 2329 2504 2608 3187 3358 3725 4110 4127
Size: 357 175 104 579 171 367 385 17
Cuts at: 4127 5579 5665 5677 6023 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 579 385 367 357 346 175
171 104 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 3972
Fragments arranged by size:
3972 1504 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2956 487
Size: 683 56 1730 3559
Fragments arranged by size:
3559 1730 683 56
BtsI GCAGTG_nn'
Cuts at: 2083 2552 3544 4338 4358 2083
Size: 469 992 794 20 3753
Fragments arranged by size:
3753 992 794 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 3332 3332
Size: 6028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5035 809
Size: 507 3719 1802
Fragments arranged by size:
3719 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2566 3475
Size: 54 1425 50 275 18 225 511 909
Cuts at: 3475 4364 5993 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 909 889 511 275 225 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3475 1537
Size: 275 1663 4090
Fragments arranged by size:
4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3958 1353
Size: 2605 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4615 4989 5135 387
Size: 1064 138 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 4091 4688 5475 5743 1239
Size: 393 495 1964 597 787 268 1524
Fragments arranged by size:
1964 1524 787 597 495 393 268
EcoRI G'AATT_C
Cuts at: 3337 3715 3337
Size: 378 5650
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 3313 5175 1678
Size: 1635 1862 2531
Fragments arranged by size:
2531 1862 1635
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 2659 2691 4669
Size: 32 135 32 943 32 112 32 1978
Cuts at: 4669 4701 4743 4775 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 943 135 112 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2025 3372 3618 669
Size: 988 281 87 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6028
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5142 5326 1305
Size: 3837 184 2007
Fragments arranged by size:
3837 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2568 10
Size: 54 1425 343 736 3470
Fragments arranged by size:
3470 1425 736 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2678 2712 3228 3986 4345 4504
Size: 648 1408 104 34 516 758 359 159
Cuts at: 4504 518
Size: 2042
Fragments arranged by size:
2042 1408 758 648 516 359 159 104
34
MspA1I CmG'CkG
Cuts at: 1606 3444 4121 5269 5514 1606
Size: 1838 677 1148 245 2120
Fragments arranged by size:
2120 1838 1148 677 245
NaeI GCC'GGC
Cuts at: 2034 2034
Size: 6028
NcoI C'CATG_G
Cuts at: 513 1065 2569 513
Size: 552 1504 3972
Fragments arranged by size:
3972 1504 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6028
NgoMIV G'CCGG_C
Cuts at: 2032 2032
Size: 6028
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 2050 3386 4931 1132
Size: 97 821 1336 1545 2229
Fragments arranged by size:
2229 1545 1336 821 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6028
PciI A'CATG_T
Cuts at: 4927 4927
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2294 3306 1108
Size: 1186 1012 3830
Fragments arranged by size:
3830 1186 1012
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2703 2735 4097 4129 5610 5642 2703
Size: 32 1362 32 1481 32 3089
Fragments arranged by size:
3089 1481 1362 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 2844 3598 2844
Size: 754 5274
PstI C_TGCA'G
Cuts at: 830 3380 830
Size: 2550 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
SalI G'TCGA_C
Cuts at: 1936 2023 3370 1936
Size: 87 1347 4594
Fragments arranged by size:
4594 1347 87
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SfcI C'TryA_G
Cuts at: 826 1045 2349 2496 3376 4511 5192 5383 826
Size: 219 1304 147 880 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 880 681 219 191 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 2032 1700
Size: 332 5696
SmaI CCC'GGG
Cuts at: 1872 3355 1872
Size: 1483 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 4132 5033 5295 820
Size: 197 926 324 1865 901 262 1553
Fragments arranged by size:
1865 1553 926 901 324 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 2243 2688 3952 5
Size: 47 2191 445 1264 2081
Fragments arranged by size:
2191 2081 1264 445 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6028
StyI C'CwwG_G
Cuts at: 513 1065 2569 3467 513
Size: 552 1504 898 3074
Fragments arranged by size:
3074 1504 898 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2735 4055 4072 4225 4410 4855 325
Size: 1255 1155 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 1155 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3206 4274 5986
Size: 274 80 33 51 1443 1229 1068 1712
Cuts at: 5986 96
Size: 138
Fragments arranged by size:
1712 1443 1229 1068 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5988 6020 5988
Size: 32 5996
Tth111I GACn'n_nGTC
Cuts at: 1316 2126 1316
Size: 810 5218
XbaI T'CTAG_A
Cuts at: 2507 3364 2507
Size: 857 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 6028
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 3353 1870
Size: 1483 4545
XmnI GAAnn'nnTTC
Cuts at: 4157 4157
Size: 6028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI