Restriction map for
pFN21AE2514
(Circular) (Six-base) MAPSORT of: pFN21AE2514.seq Check: 455 from: 1 to: 7387
pFN21AE2514 7387 bp
With 182 enzymes: SgfI *
March 20, 2014 17:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2391 2391
Size: 7387
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4662 5197 278
Size: 53 83 186 936 3126 535 2468
Fragments arranged by size:
3126 2468 936 535 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4599 4730 1937
Size: 2662 131 4594
Fragments arranged by size:
4594 2662 131
Acc65I G'GTAC_C
Cuts at: 4708 4708
Size: 7387
AclI AA'CG_TT
Cuts at: 1563 5514 5887 1563
Size: 3951 373 3063
Fragments arranged by size:
3951 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2938 3655 3896 4576 5450 6834 7102 1632
Size: 1306 717 241 680 874 1384 268 1917
Fragments arranged by size:
1917 1384 1306 874 717 680 268 241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7190
AflIII A'CryG_T
Cuts at: 1203 3390 4122 4392 4497 6286 7263 1203
Size: 2187 732 270 105 1789 977 1327
Fragments arranged by size:
2187 1789 1327 977 732 270 105
AgeI A'CCGG_T
Cuts at: 6218 6218
Size: 7387
AhdI GACnn_n'nnGTC
Cuts at: 6116 6116
Size: 7387
AleI CACnn'nnGTG
Cuts at: 1166 3030 1166
Size: 1864 5523
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2452 3048 3182 1184
Size: 1268 596 134 5389
Fragments arranged by size:
5389 1268 596 134
AlwNI CAG_nnn'CTG
Cuts at: 2069 4138 5130 6702 2069
Size: 2069 992 1572 2754
Fragments arranged by size:
2754 2069 1572 992
ApaI G_GGCC'C
Cuts at: 2894 4355 2894
Size: 1461 5926
ApaLI G'TGCA_C
Cuts at: 3335 4442 5444 6600 3335
Size: 1107 1002 1156 4122
Fragments arranged by size:
4122 1156 1107 1002
ApoI r'AATT_y
Cuts at: 1493 2845 4696 4922 5074 7183 1493
Size: 1352 1851 226 152 2109 1697
Fragments arranged by size:
2109 1851 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5941 160
Size: 872 4909 1606
Fragments arranged by size:
4909 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
AvaI C'yCGr_G
Cuts at: 1870 1943 2578 2759 2810 2911 3148 3497 3659
Size: 73 635 181 51 101 237 349 162
Cuts at: 3659 3725 3733 4001 4040 4271 4297 4348 4355
Size: 66 8 268 39 231 26 51 7
Cuts at: 4355 4712 1870
Size: 357 4545
Fragments arranged by size:
4545 635 357 349 268 237 231 181
162 101 73 66 51 51 39 26
8 7
AvrII C'CTAG_G
Cuts at: 3814 3814
Size: 7387
BamHI G'GATC_C
Cuts at: 2352 4717 5084 2352
Size: 2365 367 4655
Fragments arranged by size:
4655 2365 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 3506 3721 3866 3986
Size: 325 217 627 474 1245 215 145 120
Cuts at: 3986 4708 6163 618
Size: 722 1455 1842
Fragments arranged by size:
1842 1455 1245 722 627 474 325 217
215 145 120
BanII G_rGCy'C
Cuts at: 1391 2055 2322 2760 2894 3015 3480 3504 3843
Size: 664 267 438 134 121 465 24 339
Cuts at: 3843 3996 4008 4047 4355 4706 1391
Size: 153 12 39 308 351 4072
Fragments arranged by size:
4072 664 465 438 351 339 308 267
153 134 121 39 24 12
BbeI G_GCGC'C
Cuts at: 3870 3870
Size: 7387
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 3434 4541 928
Size: 971 207 1328 1107 3774
Fragments arranged by size:
3774 1328 1107 971 207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3313 3347 4188 4222 5578 5612 1903
Size: 34 1376 34 841 34 1356 34 3678
Fragments arranged by size:
3678 1376 1356 841 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5279 6489 5279
Size: 1210 6177
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 6433
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2440 2812 2937
Size: 107 122 71 631 605 768 372 125
Cuts at: 2937 4299 4308 4461 5998 136
Size: 1362 9 153 1537 1525
Fragments arranged by size:
1537 1525 1362 768 631 605 372 153
125 122 107 71 9
BglII A'GATC_T
Cuts at: 7382 7382
Size: 7387
BlpI GC'TnA_GC
Cuts at: 3689 4804 3689
Size: 1115 6272
Bme1580I G_kGCm'C
Cuts at: 1790 2524 2894 2942 3339 3726 4355 4446 5448
Size: 734 370 48 397 387 629 91 1002
Cuts at: 5448 6604 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1002 734 629 397 387 370
91 48
BmgBI CAC'GTC
Cuts at: 1318 4221 1318
Size: 2903 4484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 5118 6076
Size: 416 53 83 399 24 1607 2094 958
Cuts at: 6076 442
Size: 1753
Fragments arranged by size:
2094 1753 1607 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7387
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2774 3662 4106 4631 6047 1239
Size: 893 642 888 444 525 1416 2579
Fragments arranged by size:
2579 1416 893 888 642 525 444
Bpu10I CC'TnA_GC
Cuts at: 2321 3215 2321
Size: 894 6493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3398 3518 5512 6377 6675 3398
Size: 120 1994 865 298 4110
Fragments arranged by size:
4110 1994 865 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2581 6050 882
Size: 617 1082 3469 2219
Fragments arranged by size:
3469 2219 1082 617
BsaAI yAC'GTr
Cuts at: 493 1204 4395 5178 493
Size: 711 3191 783 2702
Fragments arranged by size:
3191 2702 783 711
BsaBI GATnn'nnATC
Cuts at: 2351 7381 2351
Size: 5030 2357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3867 4154 4659 5194
Size: 53 83 186 936 2334 287 505 535
Cuts at: 5194 5576 6211 275
Size: 382 635 1451
Fragments arranged by size:
2334 1451 936 635 535 505 382 287
186 83 53
BsaWI w'CCGG_w
Cuts at: 5820 6218 6492 6639 7050 5820
Size: 398 274 147 411 6157
Fragments arranged by size:
6157 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2599 2629 1178
Size: 30 165 30 1196 30 5936
Fragments arranged by size:
5936 1196 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2296 2470 3017 3611 3614 3623 3626
Size: 949 161 174 547 594 3 9 3
Cuts at: 3626 3629 3632 3635 3638 3641 3644 4696 1186
Size: 3 3 3 3 3 3 1052 3877
Fragments arranged by size:
3877 1052 949 594 547 174 161 9
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2448 3021 3194 3580 3787 4034 4364 6590 7086
Size: 573 173 386 207 247 330 2226 496
Cuts at: 7086 2448
Size: 2749
Fragments arranged by size:
2749 2226 573 496 386 330 247 207
173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2458 1163
Size: 573 722 6092
Fragments arranged by size:
6092 722 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3463 3486 3543 4837 5598
Size: 275 186 1042 420 23 57 1294 761
Cuts at: 5598 6626 1540
Size: 1028 2301
Fragments arranged by size:
2301 1294 1042 1028 761 420 275 186
57 23
BsiHKAI G_wGCw'C
Cuts at: 2055 2232 2322 3221 3339 3398 3843 4233 4446
Size: 177 90 899 118 59 445 390 213
Cuts at: 4446 4644 5448 5533 6604 2055
Size: 198 804 85 1071 2838
Fragments arranged by size:
2838 1071 899 804 445 390 213 198
177 118 90 85 59
BsmI GAATG_Cn'
Cuts at: 4898 4991 4898
Size: 93 7294
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2232 2322 2524 2760 2894 2942
Size: 399 265 177 90 202 236 134 48
Cuts at: 2942 3015 3221 3339 3398 3480 3504 3726 3843
Size: 73 206 118 59 82 24 222 117
Cuts at: 3843 3996 4008 4047 4233 4355 4446 4644 4706
Size: 153 12 39 186 122 91 198 62
Cuts at: 4706 5448 5533 6604 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 265 236 222 206
202 198 186 177 153 134 122 118
117 91 90 85 82 73 62 59
48 39 24 12
BspEI T'CCGG_A
Cuts at: 7050 7050
Size: 7387
BspHI T'CATG_A
Cuts at: 2841 4167 5276 7006 2841
Size: 1326 1109 1730 3222
Fragments arranged by size:
3222 1730 1326 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2175 2391 2883 4287 4742 844
Size: 1331 216 492 1404 455 3489
Fragments arranged by size:
3489 1404 1331 492 455 216
BsrBI CCG'CTC
Cuts at: 1394 1823 2809 2839 3465 4468 5274 1394
Size: 429 986 30 626 1003 806 3507
Fragments arranged by size:
3507 1003 986 806 626 429 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 2424 5882 6056 65
Size: 1183 1176 3458 174 1396
Fragments arranged by size:
3458 1396 1183 1176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2338 2764 3171 3339 3446 3458 3654
Size: 110 528 426 407 168 107 12 196
Cuts at: 3654 4446 4460 6031 6218 1700
Size: 792 14 1571 187 2869
Fragments arranged by size:
2869 1571 792 528 426 407 196 187
168 110 107 14 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7387
BssHII G'CGCG_C
Cuts at: 1925 2565 2567 4503 1925
Size: 640 2 1936 4809
Fragments arranged by size:
4809 1936 640 2
BssSI C'ACGA_G
Cuts at: 3563 3587 5447 6459 3563
Size: 24 1860 1012 4491
Fragments arranged by size:
4491 1860 1012 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2679 3288 794
Size: 1885 609 4893
Fragments arranged by size:
4893 1885 609
BstBI TT'CG_AA
Cuts at: 6191 6191
Size: 7387
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7387
BstXI CCAn_nnnn'nTGG
Cuts at: 3517 3763 3517
Size: 246 7141
BstYI r'GATC_y
Cuts at: 1972 2352 4717 5084 5469 5486 6938 7024 7036
Size: 380 2365 367 385 17 1452 86 12
Cuts at: 7036 7382 1972
Size: 346 1977
Fragments arranged by size:
2365 1977 1452 385 380 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2786 2823 3063 3084 3154 513
Size: 552 1721 37 240 21 70 4746
Fragments arranged by size:
4746 1721 552 240 70 37 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6648
Fragments arranged by size:
6648 683 56
BtsI GCAGTG_nn'
Cuts at: 2249 3821 4139 4903 5697 5717 2249
Size: 1572 318 764 794 20 3919
Fragments arranged by size:
3919 1572 794 764 318 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7387
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7352
DraI TTT'AAA
Cuts at: 4691 4691
Size: 7387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6394 809
Size: 507 5078 1802
Fragments arranged by size:
5078 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 2766 3066
Size: 54 1425 50 275 18 349 587 300
Cuts at: 3066 3456 3460 3483 3540 3753 3846 4314 4834
Size: 390 4 23 57 213 93 468 520
Cuts at: 4834 5723 7352 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 587 520 468 390 349
300 275 213 93 57 54 50 43
23 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3460 3483 3540 4834 1537
Size: 275 1648 23 57 1294 4090
Fragments arranged by size:
4090 1648 1294 275 57 23
EarI CTCTTCn'nnn_
Cuts at: 1353 2957 4013 5317 1353
Size: 1604 1056 1304 3423
Fragments arranged by size:
3423 1604 1304 1056
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3545 4465 5974 6348 6494 387
Size: 1064 138 1956 920 1509 374 146 1280
Fragments arranged by size:
1956 1509 1280 1064 920 374 146 138
EcoICRI GAG'CTC
Cuts at: 2053 2320 3841 2053
Size: 267 1521 5599
Fragments arranged by size:
5599 1521 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2774 2938 3655 3662 3896 4106
Size: 393 500 642 164 717 7 234 210
Cuts at: 4106 4576 4631 5450 6047 6834 7102 1239
Size: 470 55 819 597 787 268 1524
Fragments arranged by size:
1524 819 787 717 642 597 500 470
393 268 234 210 164 55 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 2285
Size: 7387
EcoO109I rG'GnC_Cy
Cuts at: 2278 2891 3529 3730 4081 4174 4276 4352 2278
Size: 613 638 201 351 93 102 76 5313
Fragments arranged by size:
5313 638 613 351 201 102 93 76
EcoRI G'AATT_C
Cuts at: 4696 5074 4696
Size: 378 7009
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 4195 2707
Size: 1488 5899
FseI GG_CCGG'CC
Cuts at: 2768 3462 2768
Size: 694 6693
FspI TGC'GCA
Cuts at: 2882 5893 2882
Size: 3011 4376
HaeII r_GCGC'y
Cuts at: 1678 2532 3421 3764 3782 3870 4056 6534 1678
Size: 854 889 343 18 88 186 2478 2531
Fragments arranged by size:
2531 2478 889 854 343 186 88 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 3464 3496 4220
Size: 32 135 32 1027 32 833 32 724
Cuts at: 4220 4252 6028 6060 6102 6134 1373
Size: 32 1776 32 42 32 2626
Fragments arranged by size:
2626 1776 1027 833 724 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3958 4731 4977 669
Size: 988 281 2020 773 246 3079
Fragments arranged by size:
3079 2020 988 773 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7387
HpaI GTT'AAC
Cuts at: 4977 4977
Size: 7387
KasI G'GCGC_C
Cuts at: 3866 3866
Size: 7387
KpnI G_GTAC'C
Cuts at: 4712 4712
Size: 7387
MfeI C'AATT_G
Cuts at: 4986 4986
Size: 7387
MluI A'CGCG_T
Cuts at: 3390 4497 7263 3390
Size: 1107 2766 3514
Fragments arranged by size:
3514 2766 1107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3492 6501 6685 1305
Size: 2187 3009 184 2007
Fragments arranged by size:
3009 2187 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2181 3068 10
Size: 54 1425 343 349 887 4329
Fragments arranged by size:
4329 1425 887 349 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3030 4070 5345 5704 5863 518
Size: 648 1864 1040 1275 359 159 2042
Fragments arranged by size:
2042 1864 1275 1040 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2452 2459 2788 3156 3229 3379 4030 4308
Size: 846 7 329 368 73 150 651 278
Cuts at: 4308 4320 4569 4803 5480 6628 6873 1606
Size: 12 249 234 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 677 651 368 329 278
249 245 234 150 73 12 7
NaeI GCC'GGC
Cuts at: 2766 3448 3460 3656 2766
Size: 682 12 196 6497
Fragments arranged by size:
6497 682 196 12
NarI GG'CG_CC
Cuts at: 3867 3867
Size: 7387
NcoI C'CATG_G
Cuts at: 513 1065 3063 3084 513
Size: 552 1998 21 4816
Fragments arranged by size:
4816 1998 552 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7387
NgoMIV G'CCGG_C
Cuts at: 2764 3446 3458 3654 2764
Size: 682 12 196 6497
Fragments arranged by size:
6497 682 196 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7387
NotI GC'GGCC_GC
Cuts at: 4834 4834
Size: 7387
NspI r_CATG'y
Cuts at: 1132 1229 2682 3439 4745 6290 1132
Size: 97 1453 757 1306 1545 2229
Fragments arranged by size:
2229 1545 1453 1306 757 97
PasI CC'CwG_GG
Cuts at: 1348 2524 1348
Size: 1176 6211
PciI A'CATG_T
Cuts at: 6286 6286
Size: 7387
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2977 1108
Size: 1869 5518
PmeI GTTT'AAAC
Cuts at: 4691 4691
Size: 7387
PmlI CAC'GTG
Cuts at: 4395 4395
Size: 7387
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5456 5488 6969 7001 4112
Size: 32 1312 32 1481 32 4498
Fragments arranged by size:
4498 1481 1312 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3730 4081 4174 4276 3730
Size: 351 93 102 6841
Fragments arranged by size:
6841 351 102 93
PshAI GACnn'nnGTC
Cuts at: 1613 2106 1613
Size: 493 6894
PsiI TTA'TAA
Cuts at: 4957 4957
Size: 7387
PspOMI G'GGCC_C
Cuts at: 2890 4351 2890
Size: 1461 5926
PstI C_TGCA'G
Cuts at: 830 2564 2718 4430 4739 830
Size: 1734 154 1712 309 3478
Fragments arranged by size:
3478 1734 1712 309 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7387
PvuII CAG'CTG
Cuts at: 2452 3379 2452
Size: 927 6460
SacI G_AGCT'C
Cuts at: 2055 2322 3843 2055
Size: 267 1521 5599
Fragments arranged by size:
5599 1521 267
SacII CC_GC'GG
Cuts at: 2789 3157 2789
Size: 368 7019
SalI G'TCGA_C
Cuts at: 1936 4729 1936
Size: 2793 4594
SanDI GG'GwC_CC
Cuts at: 3730 3730
Size: 7387
SapI GCTCTTCn'nnn_
Cuts at: 4013 4013
Size: 7387
SbfI CC_TGCA'GG
Cuts at: 4739 4739
Size: 7387
ScaI AGT'ACT
Cuts at: 4403 5635 7347 4403
Size: 1232 1712 4443
Fragments arranged by size:
4443 1712 1232
SfcI C'TryA_G
Cuts at: 826 1045 2560 2714 4426 4600 4735 5870 6551
Size: 219 1515 154 1712 174 135 1135 681
Cuts at: 6551 6742 826
Size: 191 1471
Fragments arranged by size:
1712 1515 1471 1135 681 219 191 174
154 135
SfiI GGCCn_nnn'nGGCC
Cuts at: 2440 4299 2440
Size: 1859 5528
SfoI GGC'GCC
Cuts at: 3868 3868
Size: 7387
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7387
SgrAI Cr'CCGG_yG
Cuts at: 1700 3339 4446 4460 1700
Size: 1639 1107 14 4627
Fragments arranged by size:
4627 1639 1107 14
SmaI CCC'GGG
Cuts at: 1872 2761 2913 3150 3727 3735 4003 4042 4273
Size: 889 152 237 577 8 268 39 231
Cuts at: 4273 4350 4357 4714 1872
Size: 77 7 357 4545
Fragments arranged by size:
4545 889 577 357 268 237 231 152
77 39 8 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3413 3533 5491 6392 6654 820
Size: 197 926 1470 120 1958 901 262 1553
Fragments arranged by size:
1958 1553 1470 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 5178 493
Size: 4685 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7387
SphI G_CATG'C
Cuts at: 1132 4745 1132
Size: 3613 3774
SrfI GCCC'GGGC
Cuts at: 2761 4350 2761
Size: 1589 5798
SspI AAT'ATT
Cuts at: 5 52 5311 5
Size: 47 5259 2081
Fragments arranged by size:
5259 2081 47
StuI AGG'CCT
Cuts at: 1517 2443 1517
Size: 926 6461
StyI C'CwwG_G
Cuts at: 513 1065 2202 3063 3084 3756 3814 4371 4826
Size: 552 1137 861 21 672 58 557 455
Cuts at: 4826 513
Size: 3074
Fragments arranged by size:
3074 1137 861 672 557 552 455 58
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2249 2407 3057 5414 5431 5584
Size: 1255 558 111 158 650 2357 17 153
Cuts at: 5584 5769 6214 325
Size: 185 445 1498
Fragments arranged by size:
2357 1498 1255 650 558 445 185 158
153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4125 4401 5633
Size: 274 80 33 51 1443 2148 276 1232
Cuts at: 5633 7345 96
Size: 1712 138
Fragments arranged by size:
2148 1712 1443 1232 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2230 2262 2581 2613 7347 7379 2230
Size: 32 319 32 4734 32 2238
Fragments arranged by size:
4734 2238 319 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3044 1316
Size: 1728 5659
XbaI T'CTAG_A
Cuts at: 4723 4723
Size: 7387
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7387
XmaI C'CCGG_G
Cuts at: 1870 2759 2911 3148 3725 3733 4001 4040 4271
Size: 889 152 237 577 8 268 39 231
Cuts at: 4271 4348 4355 4712 1870
Size: 77 7 357 4545
Fragments arranged by size:
4545 889 577 357 268 237 231 152
77 39 8 7
XmnI GAAnn'nnTTC
Cuts at: 3185 5516 3185
Size: 2331 5056
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4660 5195 276
Size: 53 83 186 936 3126 535 2468
Fragments arranged by size:
3126 2468 936 535 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI
PacI PfoI PspXI PsrI RsrII SexAI SwaI XcmI