Restriction map for
pFN21AE2507
(Circular) (Six-base) MAPSORT of: pFN21AE2507.seq Check: 5339 from: 1 to: 6025
pFN21AE2507 6025 bp
With 182 enzymes: SgfI *
February 14, 2014 11:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3835 278
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3368 1937
Size: 1431 4594
Acc65I G'GTAC_C
Cuts at: 3144 3346 3144
Size: 202 5823
AclI AA'CG_TT
Cuts at: 1563 4152 4525 1563
Size: 2589 373 3063
Fragments arranged by size:
3063 2589 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2861 2878 2936 3065 4088 5472 5740
Size: 527 702 17 58 129 1023 1384 268
Cuts at: 5740 1632
Size: 1917
Fragments arranged by size:
1917 1384 1023 702 527 268 129 58
17
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5828
AflIII A'CryG_T
Cuts at: 1203 4924 5901 1203
Size: 3721 977 1327
Fragments arranged by size:
3721 1327 977
AgeI A'CCGG_T
Cuts at: 4856 4856
Size: 6025
AhdI GACnn_n'nnGTC
Cuts at: 2173 2326 4754 2173
Size: 153 2428 3444
Fragments arranged by size:
3444 2428 153
AleI CACnn'nnGTG
Cuts at: 1166 3312 1166
Size: 2146 3879
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6025
AlwNI CAG_nnn'CTG
Cuts at: 2452 2680 2697 2839 3191 3768 5340 2452
Size: 228 17 142 352 577 1572 3137
Fragments arranged by size:
3137 1572 577 352 228 142 17
ApaI G_GGCC'C
Cuts at: 2073 2224 2912 2073
Size: 151 688 5186
Fragments arranged by size:
5186 688 151
ApaLI G'TGCA_C
Cuts at: 2876 3113 4082 5238 2876
Size: 237 969 1156 3663
Fragments arranged by size:
3663 1156 969 237
ApoI r'AATT_y
Cuts at: 1493 3334 3560 3712 5821 1493
Size: 1841 226 152 2109 1697
Fragments arranged by size:
2109 1841 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4579 160
Size: 872 3547 1606
Fragments arranged by size:
3547 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
AvaI C'yCGr_G
Cuts at: 1870 1943 2799 3290 3350 1870
Size: 73 856 491 60 4545
Fragments arranged by size:
4545 856 491 73 60
BamHI G'GATC_C
Cuts at: 2517 2853 3030 3355 3722 2517
Size: 336 177 325 367 4820
Fragments arranged by size:
4820 367 336 325 177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3144 3346 4801 618
Size: 325 217 627 1357 202 1455 1842
Fragments arranged by size:
1842 1455 1357 627 325 217 202
BanII G_rGCy'C
Cuts at: 1391 2073 2224 2912 3126 3344 1391
Size: 682 151 688 214 218 4072
Fragments arranged by size:
4072 688 682 218 214 151
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4216 4250 1903
Size: 34 2279 34 3678
Fragments arranged by size:
3678 2279 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3917 5127 3917
Size: 1210 4815
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6025
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 4636 136
Size: 107 122 71 631 605 484 2480 1525
Fragments arranged by size:
2480 1525 631 605 484 122 107 71
BglII A'GATC_T
Cuts at: 6020 6020
Size: 6025
BlpI GC'TnA_GC
Cuts at: 3442 3442
Size: 6025
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2224 2367 2465 2880 2912 3117 4086
Size: 283 151 143 98 415 32 205 969
Cuts at: 4086 5242 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 969 415 283 205 151 143
98 32
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 5197
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3097 3756 4714
Size: 416 53 83 399 24 1680 659 958
Cuts at: 4714 442
Size: 1753
Fragments arranged by size:
1753 1680 958 659 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 4685 1239
Size: 1043 2403 2579
Fragments arranged by size:
2579 2403 1043
Bpu10I CC'TnA_GC
Cuts at: 3120 3120
Size: 6025
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4150 5015 5313 4150
Size: 865 298 4862
Fragments arranged by size:
4862 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 3297 4688 882
Size: 617 1023 775 1391 2219
Fragments arranged by size:
2219 1391 1023 775 617
BsaAI yAC'GTr
Cuts at: 493 1204 3816 493
Size: 711 2612 2702
Fragments arranged by size:
2702 2612 711
BsaBI GATnn'nnATC
Cuts at: 2132 6019 2132
Size: 3887 2138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2046 2372 2501 3832
Size: 53 83 186 936 513 326 129 1331
Cuts at: 3832 4214 4849 275
Size: 382 635 1451
Fragments arranged by size:
1451 1331 936 635 513 382 326 186
129 83 53
BsaWI w'CCGG_w
Cuts at: 2292 4458 4856 5130 5277 5688 2292
Size: 2166 398 274 147 411 2629
Fragments arranged by size:
2629 2166 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5800
Fragments arranged by size:
5800 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3005 1186
Size: 1210 609 4206
Fragments arranged by size:
4206 1210 609
BseYI C'CCAG_C
Cuts at: 2647 3184 3209 5228 5724 2647
Size: 537 25 2019 496 2948
Fragments arranged by size:
2948 2019 537 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2101 2426 2693 2923 1163
Size: 573 324 41 325 267 230 4265
Fragments arranged by size:
4265 573 325 324 267 230 41
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2334 2391 3475 4236 5264 1540
Size: 275 186 333 57 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 333 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2607 2730 2880 3117 3126 4086 4171 5242 2607
Size: 123 150 237 9 960 85 1071 3390
Fragments arranged by size:
3390 1071 960 237 150 123 85 9
BsmI GAATG_Cn'
Cuts at: 3536 3629 3536
Size: 93 5932
BsmBI CGTCTCn'nnnn_
Cuts at: 2378 2378
Size: 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2224 2367 2465 2607 2730 2880
Size: 399 283 151 143 98 142 123 150
Cuts at: 2880 2912 3117 3126 3344 4086 4171 5242 1391
Size: 32 205 9 218 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 283 218 205 151
150 143 142 123 98 85 32 9
BspEI T'CCGG_A
Cuts at: 2292 5688 2292
Size: 3396 2629
BspHI T'CATG_A
Cuts at: 2061 3914 5644 2061
Size: 1853 1730 2442
Fragments arranged by size:
2442 1853 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2688 3380 844
Size: 1844 692 3489
Fragments arranged by size:
3489 1844 692
BsrBI CCG'CTC
Cuts at: 1394 1823 2641 3912 1394
Size: 429 818 1271 3507
Fragments arranged by size:
3507 1271 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2096 2346 4520 4694 65
Size: 1183 848 250 2174 174 1396
Fragments arranged by size:
2174 1396 1183 848 250 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2424 4669 4856 1700
Size: 110 614 2245 187 2869
Fragments arranged by size:
2869 2245 614 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6025
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6025
BssSI C'ACGA_G
Cuts at: 2466 2879 4085 5097 2466
Size: 413 1206 1012 3394
Fragments arranged by size:
3394 1206 1012 413
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2680 794
Size: 1886 4139
BstBI TT'CG_AA
Cuts at: 4829 4829
Size: 6025
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6025
BstYI r'GATC_y
Cuts at: 1972 2187 2487 2517 2853 3006 3030 3355 3722
Size: 215 300 30 336 153 24 325 367
Cuts at: 3722 4107 4124 5576 5662 5674 6020 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 336 325 300
215 153 86 30 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2081 2367 2521 2857 3229 513
Size: 552 939 77 286 154 336 372 3309
Fragments arranged by size:
3309 939 552 372 336 286 154 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5286
Fragments arranged by size:
5286 683 56
BtsI GCAGTG_nn'
Cuts at: 2308 2574 2710 3541 4335 4355 2308
Size: 266 136 831 794 20 3978
Fragments arranged by size:
3978 831 794 266 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6025
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5990
DraI TTT'AAA
Cuts at: 3329 3329
Size: 6025
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2756 3107 5032 809
Size: 507 1440 351 1925 1802
Fragments arranged by size:
1925 1802 1440 507 351
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2107 2148 2331
Size: 54 1425 50 275 18 277 41 183
Cuts at: 2331 2388 3274 3472 4361 5990 8
Size: 57 886 198 889 1629 43
Fragments arranged by size:
1629 1425 889 886 277 275 198 183
57 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2331 2388 3472 1537
Size: 275 519 57 1084 4090
Fragments arranged by size:
4090 1084 519 275 57
EarI CTCTTCn'nnn_
Cuts at: 1353 2612 2690 3955 1353
Size: 1259 78 1265 3423
Fragments arranged by size:
3423 1265 1259 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3311 4612 4986 5132 387
Size: 1064 138 1722 1301 374 146 1280
Fragments arranged by size:
1722 1301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3124 3124
Size: 6025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2282 2861 2878 2936 3065 4088
Size: 393 527 123 579 17 58 129 1023
Cuts at: 4088 4685 5472 5740 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1023 787 597 579 527 393 268
129 123 58 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2843 2207
Size: 636 5389
EcoO109I rG'GnC_Cy
Cuts at: 2032 2070 2200 2220 2221 2257 2677 2778 2836
Size: 38 130 20 1 36 420 101 58
Cuts at: 2836 2908 2909 2032
Size: 72 1 5148
Fragments arranged by size:
5148 420 130 101 72 58 38 36
20 1 1
EcoRI G'AATT_C
Cuts at: 3334 3712 3334
Size: 378 5647
FspI TGC'GCA
Cuts at: 4531 4531
Size: 6025
HaeII r_GCGC'y
Cuts at: 1678 2453 2511 2576 2648 2828 3216 5172 1678
Size: 775 58 65 72 180 388 1956 2531
Fragments arranged by size:
2531 1956 775 388 180 72 65 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2043 2075 2159 2191 2312
Size: 32 135 32 471 32 84 32 121
Cuts at: 2312 2344 2486 2518 2957 2989 4666 4698 4740
Size: 32 142 32 439 32 1677 32 42
Cuts at: 4740 4772 1373
Size: 32 2626
Fragments arranged by size:
2626 1677 471 439 142 135 121 84
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3058 3369 3615 669
Size: 988 281 1120 311 246 3079
Fragments arranged by size:
3079 1120 988 311 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6025
HpaI GTT'AAC
Cuts at: 3615 3615
Size: 6025
KpnI G_GTAC'C
Cuts at: 3148 3350 3148
Size: 202 5823
MfeI C'AATT_G
Cuts at: 3624 3624
Size: 6025
MluI A'CGCG_T
Cuts at: 5901 5901
Size: 6025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2074 2607 2865 5139 5323 1305
Size: 769 533 258 2274 184 2007
Fragments arranged by size:
2274 2007 769 533 258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 3276 10
Size: 54 1425 343 318 1126 2759
Fragments arranged by size:
2759 1425 1126 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2981 3312 3983 4342 4501 518
Size: 648 1815 331 671 359 159 2042
Fragments arranged by size:
2042 1815 671 648 359 331 159
MspA1I CmG'CkG
Cuts at: 1606 2083 2369 2523 2859 3188 3441 4118 5266
Size: 477 286 154 336 329 253 677 1148
Cuts at: 5266 5511 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 477 336 329 286 253
245 154
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4534
Fragments arranged by size:
4534 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6025
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6025
NotI GC'GGCC_GC
Cuts at: 3472 3472
Size: 6025
NspI r_CATG'y
Cuts at: 1132 1229 3383 4928 1132
Size: 97 2154 1545 2229
Fragments arranged by size:
2229 2154 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6025
PciI A'CATG_T
Cuts at: 4924 4924
Size: 6025
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3087 1108
Size: 1979 4046
PmeI GTTT'AAAC
Cuts at: 3329 3329
Size: 6025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 5607 5639 4094
Size: 32 1481 32 4480
Fragments arranged by size:
4480 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2257 2677 2200
Size: 57 420 5548
Fragments arranged by size:
5548 420 57
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6025
PsiI TTA'TAA
Cuts at: 3595 3595
Size: 6025
PspOMI G'GGCC_C
Cuts at: 2069 2220 2908 2069
Size: 151 688 5186
Fragments arranged by size:
5186 688 151
PstI C_TGCA'G
Cuts at: 830 2658 2676 3377 830
Size: 1828 18 701 3478
Fragments arranged by size:
3478 1828 701 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6025
PvuII CAG'CTG
Cuts at: 3188 3188
Size: 6025
SacI G_AGCT'C
Cuts at: 3126 3126
Size: 6025
SacII CC_GC'GG
Cuts at: 2084 2370 2524 2860 2084
Size: 286 154 336 5249
Fragments arranged by size:
5249 336 286 154
SalI G'TCGA_C
Cuts at: 1936 3367 1936
Size: 1431 4594
SapI GCTCTTCn'nnn_
Cuts at: 2612 2690 2612
Size: 78 5947
SbfI CC_TGCA'GG
Cuts at: 2676 3377 2676
Size: 701 5324
ScaI AGT'ACT
Cuts at: 4273 5985 4273
Size: 1712 4313
SfcI C'TryA_G
Cuts at: 826 1045 2654 2672 3139 3373 4508 5189 5380
Size: 219 1609 18 467 234 1135 681 191
Cuts at: 5380 826
Size: 1471
Fragments arranged by size:
1609 1471 1135 681 467 234 219 191
18
SfiI GGCCn_nnn'nGGCC
Cuts at: 2156 2156
Size: 6025
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6025
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6025
SmaI CCC'GGG
Cuts at: 1872 2801 3352 1872
Size: 929 551 4545
Fragments arranged by size:
4545 929 551
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4129 5030 5292 820
Size: 197 926 2186 901 262 1553
Fragments arranged by size:
2186 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3816 493
Size: 3323 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6025
SphI G_CATG'C
Cuts at: 1132 3383 1132
Size: 2251 3774
SspI AAT'ATT
Cuts at: 5 52 3949 5
Size: 47 3897 2081
Fragments arranged by size:
3897 2081 47
StuI AGG'CCT
Cuts at: 1517 2588 1517
Size: 1071 4954
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 2583 3464 513
Size: 552 939 528 51 881 3074
Fragments arranged by size:
3074 939 881 552 528 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2941 3098 4052 4069 4222 4407 4852
Size: 1255 1361 157 954 17 153 185 445
Cuts at: 4852 325
Size: 1498
Fragments arranged by size:
1498 1361 1255 954 445 185 157 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4271 5983 96
Size: 274 80 33 51 1443 2294 1712 138
Fragments arranged by size:
2294 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2287 2319 2991 3023 5985 6017 2287
Size: 32 672 32 2962 32 2295
Fragments arranged by size:
2962 2295 672 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2550 1316
Size: 1234 4791
XbaI T'CTAG_A
Cuts at: 3361 3361
Size: 6025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 3319 2257
Size: 1062 4963
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6025
XmaI C'CCGG_G
Cuts at: 1870 2799 3350 1870
Size: 929 551 4545
Fragments arranged by size:
4545 929 551
XmnI GAAnn'nnTTC
Cuts at: 4154 4154
Size: 6025
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3833 276
Size: 53 83 186 936 2299 2468
Fragments arranged by size:
2468 2299 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI
SexAI SfoI SrfI SwaI