Restriction map for
pFN21AE2501
(Circular) (Six-base) MAPSORT of: pFN21AE2501.seq Check: 2308 from: 1 to: 5671
pFN21AE2501 5671 bp
With 182 enzymes: SgfI *
February 16, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 3014 1937
Size: 172 905 4594
Fragments arranged by size:
4594 905 172
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2797 3734 5118 5386 1632
Size: 1165 937 1384 268 1917
Fragments arranged by size:
1917 1384 1165 937 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 2067 4570 5547 1203
Size: 864 2503 977 1327
Fragments arranged by size:
2503 1327 977 864
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 2402 1184
Size: 969 249 4453
Fragments arranged by size:
4453 969 249
AlwNI CAG_nnn'CTG
Cuts at: 2413 2524 2732 3414 4986 2413
Size: 111 208 682 1572 3098
Fragments arranged by size:
3098 1572 682 208 111
ApaI G_GGCC'C
Cuts at: 2718 2718
Size: 5671
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2980 3206 3358 5467 1493
Size: 1487 226 152 2109 1697
Fragments arranged by size:
2109 1697 1487 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2710 2959 2996 1870
Size: 73 767 249 37 4545
Fragments arranged by size:
4545 767 249 73 37
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2992 4447 618
Size: 325 217 627 1205 1455 1842
Fragments arranged by size:
1842 1455 1205 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2292 2718 2990 1391
Size: 901 426 272 4072
Fragments arranged by size:
4072 901 426 272
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 2356 3088 2356
Size: 732 4939
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2687 2718 3732 4888 1790
Size: 601 296 31 1014 1156 2573
Fragments arranged by size:
2573 1156 1014 601 296 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2977 3402 4360
Size: 416 53 83 399 24 1560 425 958
Cuts at: 4360 442
Size: 1753
Fragments arranged by size:
1753 1560 958 425 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4331 1239
Size: 3092 2579
Bpu10I CC'TnA_GC
Cuts at: 2946 2946
Size: 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3796 4661 4959 2909
Size: 887 865 298 3621
Fragments arranged by size:
3621 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2068 3462 493
Size: 711 864 1394 2702
Fragments arranged by size:
2702 1394 864 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2922 2952 1178
Size: 30 165 30 1519 30 3897
Fragments arranged by size:
3897 1519 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5671
BseYI C'CCAG_C
Cuts at: 2406 4874 5370 2406
Size: 2468 496 2707
Fragments arranged by size:
2707 2468 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 1163
Size: 573 1062 4036
Fragments arranged by size:
4036 1062 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2292 3732 3817 4888 2292
Size: 1440 85 1071 3075
Fragments arranged by size:
3075 1440 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2391 2687 2718 2990 3732 3817
Size: 399 502 99 296 31 272 742 85
Cuts at: 3817 4888 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 296 272 99
85 31
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 2850 3560 5290 2850
Size: 710 1730 3231
Fragments arranged by size:
3231 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2770 3026 844
Size: 1926 256 3489
Fragments arranged by size:
3489 1926 256
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2088 4166 4340 65
Size: 1183 840 2078 174 1396
Fragments arranged by size:
2078 1396 1183 840 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 2732 794
Size: 1730 208 3733
Fragments arranged by size:
3733 1730 208
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2755 2755
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2193 2520 3001 3368 3753 3770 5222 5308
Size: 221 327 481 367 385 17 1452 86
Cuts at: 5308 5320 5666 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 481 385 367 346 327 221
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2676 2676
Size: 5671
BtgI C'CryG_G
Cuts at: 513 1065 2004 2167 2754 513
Size: 552 939 163 587 3430
Fragments arranged by size:
3430 939 587 552 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2570 2730 3187 3981 4001 2570
Size: 160 457 794 20 4240
Fragments arranged by size:
4240 794 457 160 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2263 2494 2865
Size: 54 1425 50 275 18 433 231 371
Cuts at: 2865 3118 4007 5636 8
Size: 253 889 1629 43
Fragments arranged by size:
1629 1425 889 433 371 275 253 231
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2150 3601 1353
Size: 797 1451 3423
Fragments arranged by size:
3423 1451 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2290 2290
Size: 5671
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2797 3734 4331 5118 5386 1239
Size: 393 1165 937 597 787 268 1524
Fragments arranged by size:
1524 1165 937 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2852 2852
Size: 5671
EcoO109I rG'GnC_Cy
Cuts at: 2172 2714 2715 2837 2845 2857 2172
Size: 542 1 122 8 12 4986
Fragments arranged by size:
4986 542 122 12 8 1
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 4818 1678
Size: 3140 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2387 2419 4312 4344 4386
Size: 32 135 32 815 32 1893 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 1893 815 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2965 4785 4969 1305
Size: 1660 1820 184 2007
Fragments arranged by size:
2007 1820 1660 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2265 2496 10
Size: 54 1425 343 433 231 3185
Fragments arranged by size:
3185 1425 433 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2622 2906 3629 3988 4147 518
Size: 648 1218 238 284 723 359 159 2042
Fragments arranged by size:
2042 1218 723 648 359 284 238 159
MspA1I CmG'CkG
Cuts at: 1606 2821 3087 3764 4912 5157 1606
Size: 1215 266 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 677 266 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2167 2754 513
Size: 552 939 163 587 3430
Fragments arranged by size:
3430 939 587 552 163
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 3029 4574 1132
Size: 97 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2172 2172
Size: 5671
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PspOMI G'GGCC_C
Cuts at: 2714 2714
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2529 2728 3023 830
Size: 1699 199 295 3478
Fragments arranged by size:
3478 1699 295 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2821 2821
Size: 5671
SacI G_AGCT'C
Cuts at: 2292 2292
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SapI GCTCTTCn'nnn_
Cuts at: 2150 2150
Size: 5671
SbfI CC_TGCA'GG
Cuts at: 2529 3023 2529
Size: 494 5177
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2314 2525 2724 3019 4154 4835 5026
Size: 219 1269 211 199 295 1135 681 191
Cuts at: 5026 826
Size: 1471
Fragments arranged by size:
1471 1269 1135 681 295 219 211 199
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2924 2959 3775 4676 4938 820
Size: 197 926 981 35 816 901 262 1553
Fragments arranged by size:
1553 981 926 901 816 262 197 35
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2004 2167 2502 2754 2840 3110 513
Size: 552 939 163 335 252 86 270 3074
Fragments arranged by size:
3074 939 552 335 270 252 163 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3698 3715 3868 4053 4498 325
Size: 1255 2118 17 153 185 445 1498
Fragments arranged by size:
2118 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2902 2902
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 2959 1943
Size: 1016 4655
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2060 3800 2060
Size: 1740 3931
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI