Restriction map for
pFN21AE2499
(Circular) (Six-base) MAPSORT of: pFN21AE2499.seq Check: 2098 from: 1 to: 18427
pFN21AE2499 18427 bp
With 182 enzymes: SgfI *
July 4, 2020 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4525 6580 7840 8404 8623 8899 9931 12742 15401
Size: 2055 1260 564 219 276 1032 2811 2659
Cuts at: 15401 4525
Size: 7551
Fragments arranged by size:
7551 2811 2659 2055 1260 1032 564 276
219
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10797 14477 16237 278
Size: 53 83 186 936 9261 3680 1760 2468
Fragments arranged by size:
9261 3680 2468 1760 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 10770 13170 15770 1937
Size: 8833 2400 2600 4594
Fragments arranged by size:
8833 4594 2600 2400
Acc65I G'GTAC_C
Cuts at: 2607 15748 2607
Size: 13141 5286
AclI AA'CG_TT
Cuts at: 1563 16554 16927 1563
Size: 14991 373 3063
Fragments arranged by size:
14991 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2061 2945 3071 3251 3581 4106 4259 4487
Size: 429 884 126 180 330 525 153 228
Cuts at: 4487 4856 5501 7895 8456 8741 10214 11237 11465
Size: 369 645 2394 561 285 1473 1023 228
Cuts at: 11465 11983 12550 14054 14210 14468 15227 16490 17874
Size: 518 567 1504 156 258 759 1263 1384
Cuts at: 17874 18142 1632
Size: 268 1917
Fragments arranged by size:
2394 1917 1504 1473 1384 1263 1023 884
759 645 567 561 525 518 429 369
330 285 268 258 228 228 180 156
153 126
AfeI AGC'GCT
Cuts at: 5036 5612 5645 6578 6866 6983 8876 9223 9299
Size: 576 33 933 288 117 1893 347 76
Cuts at: 9299 9605 10694 11099 15071 15242 5036
Size: 306 1089 405 3972 171 8221
Fragments arranged by size:
8221 3972 1893 1089 933 576 405 347
306 288 171 117 76 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 18230
AflIII A'CryG_T
Cuts at: 1203 6883 17326 18303 1203
Size: 5680 10443 977 1327
Fragments arranged by size:
10443 5680 1327 977
AgeI A'CCGG_T
Cuts at: 14003 15062 17258 14003
Size: 1059 2196 15172
Fragments arranged by size:
15172 2196 1059
AhdI GACnn_n'nnGTC
Cuts at: 2830 13594 17156 2830
Size: 10764 3562 4101
Fragments arranged by size:
10764 4101 3562
AleI CACnn'nnGTG
Cuts at: 1166 2274 4557 5137 10118 10970 12752 1166
Size: 1108 2283 580 4981 852 1782 6841
Fragments arranged by size:
6841 4981 2283 1782 1108 852 580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6443 6819 9963 10514 11042 12491 14225 15482
Size: 5259 376 3144 551 528 1449 1734 1257
Cuts at: 15482 1184
Size: 4129
Fragments arranged by size:
5259 4129 3144 1734 1449 1257 551 528
376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 2988 3019 3020 2987
Size: 1 31 1 18394
Fragments arranged by size:
18394 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2039 2617 3049 3667 4306 4720 4987 4990 4999
Size: 578 432 618 639 414 267 3 9
Cuts at: 4999 5845 6358 6517 6697 7210 7279 7342 7450
Size: 846 513 159 180 513 69 63 108
Cuts at: 7450 8290 8617 8647 8719 9154 9385 9538 9556
Size: 840 327 30 72 435 231 153 18
Cuts at: 9556 9673 9727 9811 9865 9886 10057 10246 10687
Size: 117 54 84 54 21 171 189 441
Cuts at: 10687 11083 11197 11443 11476 11893 12150 12304 12364
Size: 396 114 246 33 417 257 154 60
Cuts at: 12364 12664 12994 13195 15016 15205 15262 15358 16170
Size: 300 330 201 1821 189 57 96 812
Cuts at: 16170 17742 2039
Size: 1572 2724
Fragments arranged by size:
2724 1821 1572 846 840 812 639 618
578 513 513 441 435 432 417 414
396 330 327 300 267 257 246 231
201 189 189 180 171 159 154 153
117 114 108 96 84 72 69 63
60 57 54 54 33 30 21 18
9 3
ApaI G_GGCC'C
Cuts at: 4583 5407 5497 6688 10367 11351 11596 11743 12344
Size: 824 90 1191 3679 984 245 147 601
Cuts at: 12344 12892 13350 14078 14921 15710 4583
Size: 548 458 728 843 789 7300
Fragments arranged by size:
7300 3679 1191 984 843 824 789 728
601 548 458 245 147 90
ApaLI G'TGCA_C
Cuts at: 4550 5010 5096 6522 10541 12518 16484 17640 4550
Size: 460 86 1426 4019 1977 3966 1156 5337
Fragments arranged by size:
5337 4019 3966 1977 1426 1156 460 86
ApoI r'AATT_y
Cuts at: 1493 3785 8600 15736 15962 16114 18223 1493
Size: 2292 4815 7136 226 152 2109 1697
Fragments arranged by size:
7136 4815 2292 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 16981 160
Size: 872 15949 1606
Fragments arranged by size:
15949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18427
AvaI C'yCGr_G
Cuts at: 1870 1943 2335 2352 3629 3843 4928 5459 5690
Size: 73 392 17 1277 214 1085 531 231
Cuts at: 5690 8373 8466 10367 10709 10788 11351 11463 11562
Size: 2683 93 1901 342 79 563 112 99
Cuts at: 11562 11736 12344 12406 12686 12875 13064 13115 13349
Size: 174 608 62 280 189 189 51 234
Cuts at: 13349 14078 14522 14794 15576 15658 15682 15752 1870
Size: 729 444 272 782 82 24 70 4545
Fragments arranged by size:
4545 2683 1901 1277 1085 782 729 608
563 531 444 392 342 280 272 234
231 214 189 189 174 112 99 93
82 79 73 70 62 51 24 17
BamHI G'GATC_C
Cuts at: 14837 15757 16124 14837
Size: 920 367 17140
Fragments arranged by size:
17140 920 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2418 2607 3154 3474 3624
Size: 325 217 627 631 189 547 320 150
Cuts at: 3624 3848 4657 4734 5744 6735 7193 8031 8151
Size: 224 809 77 1010 991 458 838 120
Cuts at: 8151 11026 11150 11273 11602 11679 13091 13674 13691
Size: 2875 124 123 329 77 1412 583 17
Cuts at: 13691 13877 14685 15371 15748 17203 618
Size: 186 808 686 377 1455 1842
Fragments arranged by size:
2875 1842 1455 1412 1010 991 838 809
808 686 631 627 583 547 458 377
329 325 320 224 217 189 186 150
124 123 120 77 77 17
BanII G_rGCy'C
Cuts at: 1391 4110 4583 5052 5407 5460 5497 5691 6371
Size: 2719 473 469 355 53 37 194 680
Cuts at: 6371 6688 8473 8775 9271 9351 10131 10367 11073
Size: 317 1785 302 496 80 780 236 706
Cuts at: 11073 11143 11351 11596 11670 11743 11757 11853 12036
Size: 70 208 245 74 73 14 96 183
Cuts at: 12036 12064 12122 12344 12892 13157 13350 13605 13983
Size: 28 58 222 548 265 193 255 378
Cuts at: 13983 14078 14921 15285 15710 15746 1391
Size: 95 843 364 425 36 4072
Fragments arranged by size:
4072 2719 1785 843 780 706 680 548
496 473 469 425 378 364 355 317
302 265 255 245 236 222 208 194
193 183 96 95 80 74 73 70
58 53 37 36 28 14
BbeI G_GCGC'C
Cuts at: 3628 5748 6739 8035 8155 14689 15375 3628
Size: 2120 991 1296 120 6534 686 6680
Fragments arranged by size:
6680 6534 2120 1296 991 686 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 3257 6854 7557 10149 12974 13891
Size: 971 396 962 3597 703 2592 2825 917
Cuts at: 13891 14477 928
Size: 586 4878
Fragments arranged by size:
4878 3597 2825 2592 971 962 917 703
586 396
BbvCI CC'TCA_GC
Cuts at: 2022 2817 7407 7835 8043 8355 9066 9225 9282
Size: 795 4590 428 208 312 711 159 57
Cuts at: 9282 9849 12053 12732 13032 13649 2022
Size: 567 2204 679 300 617 6800
Fragments arranged by size:
6800 4590 2204 795 711 679 617 567
428 312 300 208 159 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4763 4797 5185 5219 6244 6278 9331
Size: 34 2826 34 388 34 1025 34 3053
Cuts at: 9331 9365 10159 10193 11041 11075 16618 16652 1903
Size: 34 794 34 848 34 5543 34 3678
Fragments arranged by size:
5543 3678 3053 2826 1025 848 794 388
34 34 34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 16319 17529 16319
Size: 1210 17217
BclI T'GATC_A
Cuts at: 1548 12939 14664 1548
Size: 11391 1725 5311
Fragments arranged by size:
11391 5311 1725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3083 3762 4445
Size: 107 122 71 631 605 1411 679 683
Cuts at: 4445 4685 5441 5471 5981 6401 6489 6665 6692
Size: 240 756 30 510 420 88 176 27
Cuts at: 6692 6950 7004 7355 7397 7490 8207 8348 8738
Size: 258 54 351 42 93 717 141 390
Cuts at: 8738 8951 9911 11346 11384 11600 12159 12236 12326
Size: 213 960 1435 38 216 559 77 90
Cuts at: 12326 12377 12828 13241 13496 15146 15200 17038 136
Size: 51 451 413 255 1650 54 1838 1525
Fragments arranged by size:
1838 1650 1525 1435 1411 960 756 717
683 679 631 605 559 510 451 420
413 390 351 258 255 240 216 213
176 141 122 107 93 90 88 77
71 54 54 51 42 38 30 27
BglII A'GATC_T
Cuts at: 2520 10440 15414 18422 2520
Size: 7920 4974 3008 2525
Fragments arranged by size:
7920 4974 3008 2525
BlpI GC'TnA_GC
Cuts at: 2172 4610 4947 6138 6414 6915 8804 9231 9444
Size: 2438 337 1191 276 501 1889 427 213
Cuts at: 9444 11705 13377 15844 2172
Size: 2261 1672 2467 4755
Fragments arranged by size:
4755 2467 2438 2261 1889 1672 1191 501
427 337 276 213
Bme1580I G_kGCm'C
Cuts at: 1790 3159 3811 4554 4583 4641 4662 5014 5100
Size: 1369 652 743 29 58 21 352 86
Cuts at: 5100 5146 5407 5497 5554 6526 6688 6928 7510
Size: 46 261 90 57 972 162 240 582
Cuts at: 7510 8536 10367 10545 10560 11283 11351 11544 11596
Size: 1026 1831 178 15 723 68 193 52
Cuts at: 11596 11743 12016 12344 12522 12892 13094 13350 14078
Size: 147 273 328 178 370 202 256 728
Cuts at: 14078 14921 15306 15461 15710 16488 17644 1790
Size: 843 385 155 249 778 1156 2573
Fragments arranged by size:
2573 1831 1369 1156 1026 972 843 778
743 728 723 652 582 385 370 352
328 273 261 256 249 240 202 193
178 178 162 155 147 90 86 68
58 57 52 46 29 21 15
BmgBI CAC'GTC
Cuts at: 1318 2806 2887 3451 4792 5599 5674 10147 10567
Size: 1488 81 564 1341 807 75 4473 420
Cuts at: 10567 12544 15367 1318
Size: 1977 2823 4378
Fragments arranged by size:
4473 4378 2823 1977 1488 1341 807 564
420 81 75
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5708 12058 14130
Size: 416 53 83 399 24 4291 6350 2072
Cuts at: 14130 16158 17116 442
Size: 2028 958 1753
Fragments arranged by size:
6350 4291 2072 2028 1753 958 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 18427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 4075 4978 6655 7438 8794 11776 12136 2350
Size: 1725 903 1677 783 1356 2982 360 8641
Fragments arranged by size:
8641 2982 1725 1677 1356 903 783 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2623 2665 2753 2995 3143 3239 3278
Size: 1106 278 42 88 242 148 96 39
Cuts at: 3278 3656 3950 4036 4100 4385 4721 4892 5093
Size: 378 294 86 64 285 336 171 201
Cuts at: 5093 5306 5327 5360 5870 5921 6212 6719 6806
Size: 213 21 33 510 51 291 507 87
Cuts at: 6806 6881 6998 7187 7301 7472 7745 7823 7937
Size: 75 117 189 114 171 273 78 114
Cuts at: 7937 7943 8006 8129 8690 9209 9233 9407 9470
Size: 6 63 123 561 519 24 174 63
Cuts at: 9470 9653 9833 9908 10295 10523 10709 11066 11198
Size: 183 180 75 387 228 186 357 132
Cuts at: 11198 11654 12191 12272 12500 12686 13277 13565 14006
Size: 456 537 81 228 186 591 288 441
Cuts at: 14006 14234 14612 14857 14972 15041 15269 15497 17087
Size: 228 378 245 115 69 228 228 1590
Cuts at: 17087 1239
Size: 2579
Fragments arranged by size:
2579 1590 1106 591 561 537 519 510
507 456 441 387 378 378 357 336
294 291 288 285 278 273 245 242
228 228 228 228 228 213 201 189
186 186 183 180 174 171 171 148
132 123 117 115 114 114 96 88
87 86 81 78 75 75 69 64
63 63 51 42 39 33 24 21
6
Bpu10I CC'TnA_GC
Cuts at: 2022 2817 5399 7407 7835 8043 8355 8472 9066
Size: 795 2582 2008 428 208 312 117 594
Cuts at: 9066 9225 9282 9849 12053 12732 13032 13649 13667
Size: 159 57 567 2204 679 300 617 18
Cuts at: 13667 2022
Size: 6782
Fragments arranged by size:
6782 2582 2204 2008 795 679 617 594
567 428 312 300 208 159 117 57
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3134 3446 5333 5405 5660 5984 6977 7613 7637
Size: 312 1887 72 255 324 993 636 24
Cuts at: 7637 8798 9005 9044 9224 9482 12062 13199 15395
Size: 1161 207 39 180 258 2580 1137 2196
Cuts at: 15395 16552 17417 17715 3134
Size: 1157 865 298 3846
Fragments arranged by size:
3846 2580 2196 1887 1161 1157 1137 993
865 636 324 312 298 258 255 207
180 72 39 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3045 8669 11576 11774 11966 12390 12737
Size: 617 1546 5624 2907 198 192 424 347
Cuts at: 12737 17090 882
Size: 4353 2219
Fragments arranged by size:
5624 4353 2907 2219 1546 617 424 347
198 192
BsaAI yAC'GTr
Cuts at: 493 1204 3208 4747 6034 6886 10674 12651 13012
Size: 711 2004 1539 1287 852 3788 1977 361
Cuts at: 13012 13624 16218 493
Size: 612 2594 2702
Fragments arranged by size:
3788 2702 2594 2004 1977 1539 1287 852
711 612 361
BsaBI GATnn'nnATC
Cuts at: 3029 10961 12944 14630 18421 3029
Size: 7932 1983 1686 3791 3035
Fragments arranged by size:
7932 3791 3035 1983 1686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2865 3333 3625 5526
Size: 53 83 186 936 1332 468 292 1901
Cuts at: 5526 5745 5760 6736 8032 8152 9291 10794 11490
Size: 219 15 976 1296 120 1139 1503 696
Cuts at: 11490 11616 13722 13908 14474 14686 15087 15339 15372
Size: 126 2106 186 566 212 401 252 33
Cuts at: 15372 15606 16234 16616 17251 275
Size: 234 628 382 635 1451
Fragments arranged by size:
2106 1901 1503 1451 1332 1296 1139 976
936 696 635 628 566 468 401 382
292 252 234 219 212 186 186 126
120 83 53 33 15
BsaWI w'CCGG_w
Cuts at: 9303 14003 14982 15062 16860 17258 17532 17679 18090
Size: 4700 979 80 1798 398 274 147 411
Cuts at: 18090 9303
Size: 9640
Fragments arranged by size:
9640 4700 1798 979 411 398 274 147
80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2356 2386 2621
Size: 30 165 30 897 30 26 30 235
Cuts at: 2621 2651 3176 3206 3959 3989 7211 7241 8162
Size: 30 525 30 753 30 3222 30 921
Cuts at: 8162 8192 10763 10793 11978 12008 12176 12206 12815
Size: 30 2571 30 1185 30 168 30 609
Cuts at: 12815 12845 13090 13120 14600 14630 14663 14693 14945
Size: 30 245 30 1480 30 33 30 252
Cuts at: 14945 14975 15242 15272 1178
Size: 30 267 30 4333
Fragments arranged by size:
4333 3222 2571 1480 1185 921 897 753
609 525 267 252 245 235 168 165
33 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3035 3326 3698 3895 4007 4010 4013 4859
Size: 1849 291 372 197 112 3 3 846
Cuts at: 4859 5405 5426 5867 5897 6134 6137 6140 6170
Size: 546 21 441 30 237 3 3 30
Cuts at: 6170 6296 6314 6359 6452 6455 6548 6581 6716
Size: 126 18 45 93 3 93 33 135
Cuts at: 6716 6737 6923 6974 7046 7100 7139 7298 7301
Size: 21 186 51 72 54 39 159 3
Cuts at: 7301 7424 7427 7502 7742 7775 7871 7874 8033
Size: 123 3 75 240 33 96 3 159
Cuts at: 8033 8036 8060 8090 8093 8156 8372 8381 8465
Size: 3 24 30 3 63 216 9 84
Cuts at: 8465 8687 8720 8780 8783 8813 8903 8933 8993
Size: 222 33 60 3 30 90 30 60
Cuts at: 8993 9176 9299 9332 9521 9695 9758 9788 9812
Size: 183 123 33 189 174 63 30 24
Cuts at: 9812 9851 9866 9905 9938 10100 10610 10991 11195
Size: 39 15 39 33 162 510 381 204
Cuts at: 11195 11594 11765 11978 12587 12779 12782 12893 13481
Size: 399 171 213 609 192 3 111 588
Cuts at: 13481 13784 13856 14063 14267 14321 14387 14626 14629
Size: 303 72 207 204 54 66 239 3
Cuts at: 14629 14636 14818 14833 14836 14924 15467 15643 15646
Size: 7 182 15 3 88 543 176 3
Cuts at: 15646 1186
Size: 3967
Fragments arranged by size:
3967 1849 846 609 588 546 543 510
441 399 381 372 303 291 240 239
237 222 216 213 207 204 204 197
192 189 186 183 182 176 174 171
162 159 159 135 126 123 123 112
111 96 93 93 90 88 84 75
72 72 66 63 63 60 60 54
54 51 45 39 39 39 33 33
33 33 33 30 30 30 30 30
30 24 24 21 21 18 15 15
9 7 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 2072 2141 2167 3731 3748 4118 4480 4707 5145
Size: 69 26 1564 17 370 362 227 438
Cuts at: 5145 5191 5296 5631 7773 7927 9294 9666 10622
Size: 46 105 335 2142 154 1367 372 956
Cuts at: 10622 11493 11522 11636 12115 12599 12891 13491 13604
Size: 871 29 114 479 484 292 600 113
Cuts at: 13604 14880 15056 17630 18126 2072
Size: 1276 176 2574 496 2373
Fragments arranged by size:
2574 2373 2142 1564 1367 1276 956 871
600 496 484 479 438 372 370 362
335 292 227 176 154 114 113 105
69 46 29 26 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2495 2909 3188 3473 3500 3638
Size: 573 498 261 414 279 285 27 138
Cuts at: 3638 3752 4481 4569 4814 4847 4862 5751 6614
Size: 114 729 88 245 33 15 889 863
Cuts at: 6614 6825 8075 8279 8399 8810 9062 9320 9398
Size: 211 1250 204 120 411 252 258 78
Cuts at: 9398 9542 10358 13340 15019 15275 1163
Size: 144 816 2982 1679 256 4315
Fragments arranged by size:
4315 2982 1679 1250 889 863 816 729
573 498 414 411 285 279 261 258
256 252 245 211 204 144 138 120
114 88 78 33 27 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2656 2985 4024 7468 7899 8073
Size: 275 186 655 329 1039 3444 431 174
Cuts at: 8073 10395 11065 12919 13363 14106 14674 14731 15195
Size: 2322 670 1854 444 743 568 57 464
Cuts at: 15195 15670 15877 16638 17666 1540
Size: 475 207 761 1028 2301
Fragments arranged by size:
3444 2322 2301 1854 1039 1028 761 743
670 655 568 475 464 444 431 329
275 207 186 174 57
BsiHKAI G_wGCw'C
Cuts at: 4110 4554 4611 5014 5052 5100 5400 5460 5691
Size: 444 57 403 38 48 300 60 231
Cuts at: 5691 6371 6526 8775 9351 10017 10131 10488 10545
Size: 680 155 2249 576 666 114 357 57
Cuts at: 10545 11073 11670 11757 11853 12036 12522 13983 16488
Size: 528 597 87 96 183 486 1461 2505
Cuts at: 16488 16573 17644 4110
Size: 85 1071 4893
Fragments arranged by size:
4893 2505 2249 1461 1071 680 666 597
576 528 486 444 403 357 300 231
183 155 114 96 87 85 60 57
57 48 38
BsiWI C'GTAC_G
Cuts at: 6044 6044
Size: 18427
BsmI GAATG_Cn'
Cuts at: 6336 15938 16031 6336
Size: 9602 93 8732
Fragments arranged by size:
9602 8732 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2643 2854 5078 6782 7241 9158 9539 10706 11103
Size: 211 2224 1704 459 1917 381 1167 397
Cuts at: 11103 11431 12683 14519 14875 14906 14939 2643
Size: 328 1252 1836 356 31 33 6131
Fragments arranged by size:
6131 2224 1917 1836 1704 1252 1167 459
397 381 356 328 211 33 31
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3159 3811 4110 4554 4583 4611 4641
Size: 399 1369 652 299 444 29 28 30
Cuts at: 4641 4662 5014 5052 5100 5146 5400 5407 5460
Size: 21 352 38 48 46 254 7 53
Cuts at: 5460 5497 5554 5691 6371 6526 6688 6928 7510
Size: 37 57 137 680 155 162 240 582
Cuts at: 7510 8473 8536 8775 9271 9351 10017 10131 10367
Size: 963 63 239 496 80 666 114 236
Cuts at: 10367 10488 10545 10560 11073 11143 11283 11351 11544
Size: 121 57 15 513 70 140 68 193
Cuts at: 11544 11596 11670 11743 11757 11853 12016 12036 12064
Size: 52 74 73 14 96 163 20 28
Cuts at: 12064 12122 12344 12522 12892 13094 13157 13350 13605
Size: 58 222 178 370 202 63 193 255
Cuts at: 13605 13983 14078 14921 15285 15306 15461 15710 15746
Size: 378 95 843 364 21 155 249 36
Cuts at: 15746 16488 16573 17644 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1369 1071 963 843 742 680 666
652 582 513 496 444 399 378 370
364 352 299 255 254 249 240 239
236 222 202 193 193 178 163 162
155 155 140 137 121 114 96 95
85 80 74 73 70 68 63 63
58 57 57 53 52 48 46 38
37 36 30 29 28 28 21 21
20 15 14 7
BspEI T'CCGG_A
Cuts at: 9303 18090 9303
Size: 8787 9640
BspHI T'CATG_A
Cuts at: 11359 16316 18046 11359
Size: 4957 1730 11740
Fragments arranged by size:
11740 4957 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2991 3666 3675 3735 3888 4380 4525 5302
Size: 2147 675 9 60 153 492 145 777
Cuts at: 5302 6048 6580 6760 7840 8404 8623 8703 8899
Size: 746 532 180 1080 564 219 80 196
Cuts at: 8899 9931 10167 10686 10914 11235 12742 13086 13182
Size: 1032 236 519 228 321 1507 344 96
Cuts at: 13182 13777 14397 15401 15782 844
Size: 595 620 1004 381 3489
Fragments arranged by size:
3489 2147 1507 1080 1032 1004 777 746
675 620 595 564 532 519 492 381
344 321 236 228 219 196 180 153
145 96 80 60 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 2203 2767 3131 3223 3805 4806
Size: 429 353 27 564 364 92 582 1001
Cuts at: 4806 5593 5623 6166 6247 6733 6919 6949 6970
Size: 787 30 543 81 486 186 30 21
Cuts at: 6970 7582 7807 8026 8122 8168 8431 9199 11719
Size: 612 225 219 96 46 263 768 2520
Cuts at: 11719 12670 13306 13381 14467 14679 14733 14817 16314
Size: 951 636 75 1086 212 54 84 1497
Cuts at: 16314 1394
Size: 3507
Fragments arranged by size:
3507 2520 1497 1086 1001 951 787 768
636 612 582 564 543 486 429 364
353 263 225 219 212 186 96 92
84 81 75 54 46 30 30 27
21
BsrDI GCAATG_nn'
Cuts at: 65 1248 16922 17096 65
Size: 1183 15674 174 1396
Fragments arranged by size:
15674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2121 2679 2718 4125 4935 5128 5440
Size: 110 311 558 39 1407 810 193 312
Cuts at: 5440 5968 6423 7463 7467 10067 10320 10575 10995
Size: 528 455 1040 4 2600 253 255 420
Cuts at: 10995 11270 11650 11682 12158 12386 12914 13391 13502
Size: 275 380 32 476 228 528 477 111
Cuts at: 13502 13694 14003 14262 14756 14804 15062 15233 15266
Size: 192 309 259 494 48 258 171 33
Cuts at: 15266 15557 15569 15581 15611 17071 17258 1700
Size: 291 12 12 30 1460 187 2869
Fragments arranged by size:
2869 2600 1460 1407 1040 810 558 528
528 494 477 476 455 420 380 312
311 309 291 275 259 258 255 253
228 193 192 187 171 111 110 48
39 33 32 30 12 12 4
BsrGI T'GTAC_A
Cuts at: 96 12756 96
Size: 12660 5767
BssHII G'CGCG_C
Cuts at: 1925 6668 6953 10399 14110 1925
Size: 4743 285 3446 3711 6242
Fragments arranged by size:
6242 4743 3711 3446 285
BssSI C'ACGA_G
Cuts at: 3518 5408 10661 12314 12353 13340 16487 17499 3518
Size: 1890 5253 1653 39 987 3147 1012 4446
Fragments arranged by size:
5253 4446 3147 1890 1653 1012 987 39
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3616 3667 4306 4741 4990 5104 5656 5845
Size: 2822 51 639 435 249 114 552 189
Cuts at: 5845 6274 6358 6517 6521 8290 8308 8617 9040
Size: 429 84 159 4 1769 18 309 423
Cuts at: 9040 9520 9538 9556 11333 11476 12150 13884 15016
Size: 480 18 18 1777 143 674 1734 1132
Cuts at: 15016 794
Size: 4205
Fragments arranged by size:
4205 2822 1777 1769 1734 1132 674 639
552 480 435 429 423 309 249 189
159 143 114 84 51 18 18 18
4
BstBI TT'CG_AA
Cuts at: 17231 17231
Size: 18427
BstEII G'GTnAC_C
Cuts at: 1184 9367 10849 11920 12922 13387 13846 1184
Size: 8183 1482 1071 1002 465 459 5765
Fragments arranged by size:
8183 5765 1482 1071 1002 465 459
BstXI CCAn_nnnn'nTGG
Cuts at: 2540 4118 5338 5362 5797 2540
Size: 1578 1220 24 435 15170
Fragments arranged by size:
15170 1578 1220 435 24
BstYI r'GATC_y
Cuts at: 1972 2520 3030 5781 6381 6444 7863 9390 10440
Size: 548 510 2751 600 63 1419 1527 1050
Cuts at: 10440 14319 14837 15414 15757 16124 16509 16526 17978
Size: 3879 518 577 343 367 385 17 1452
Cuts at: 17978 18064 18076 18422 1972
Size: 86 12 346 1977
Fragments arranged by size:
3879 2751 1977 1527 1452 1419 1050 600
577 548 518 510 385 367 346 343
86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 2799 3920 10291 14250 2799
Size: 1121 6371 3959 6976
Fragments arranged by size:
6976 6371 3959 1121
BtgI C'CryG_G
Cuts at: 513 1065 4158 5371 5866 7215 8733 9993 10116
Size: 552 3093 1213 495 1349 1518 1260 123
Cuts at: 10116 10518 10560 11247 11316 11502 11811 11925 12495
Size: 402 42 687 69 186 309 114 570
Cuts at: 12495 12823 13158 13741 13764 13803 14197 14229 14271
Size: 328 335 583 23 39 394 32 42
Cuts at: 14271 14922 15592 15720 513
Size: 651 670 128 3220
Fragments arranged by size:
3220 3093 1518 1349 1260 1213 687 670
651 583 570 552 495 402 394 335
328 309 186 128 123 114 69 42
42 39 32 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2176 2313 2443 2635 5137 5238
Size: 683 56 950 137 130 192 2502 101
Cuts at: 5238 9424 11146 13873 14695 487
Size: 4186 1722 2727 822 4219
Fragments arranged by size:
4219 4186 2727 2502 1722 950 822 683
192 137 130 101 56
BtsI GCAGTG_nn'
Cuts at: 2068 2087 4199 4316 6515 6808 8654 9930 11334
Size: 19 2112 117 2199 293 1846 1276 1404
Cuts at: 11334 11871 12151 15943 16737 16757 2068
Size: 537 280 3792 794 20 3738
Fragments arranged by size:
3792 3738 2199 2112 1846 1404 1276 794
537 293 280 117 20 19
ClaI AT'CG_AT
Cuts at: 1554 14700 1554
Size: 13146 5281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3573 3608 14788 14823 529
Size: 35 3009 35 11180 35 4133
Fragments arranged by size:
11180 4133 3009 35 35 35
DraI TTT'AAA
Cuts at: 15731 15731
Size: 18427
DraIII CAC_nnn'GTG
Cuts at: 2235 3700 3815 4731 6521 10555 11539 12532 2235
Size: 1465 115 916 1790 4034 984 993 8130
Fragments arranged by size:
8130 4034 1790 1465 993 984 916 115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2024 2810 14651 15187 17434 809
Size: 507 708 786 11841 536 2247 1802
Fragments arranged by size:
11841 2247 1802 786 708 536 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2302 2562 2653
Size: 54 1425 50 275 18 472 260 91
Cuts at: 2653 2982 3210 4437 4515 4818 5442 5799 5982
Size: 329 228 1227 78 303 624 357 183
Cuts at: 5982 6171 6210 7212 7356 7389 7465 7482 7542
Size: 189 39 1002 144 33 76 17 60
Cuts at: 7542 7602 7896 8070 9252 9657 9732 9970 10392
Size: 60 294 174 1182 405 75 238 422
Cuts at: 10392 10432 11244 11652 12264 12916 13497 13696 13998
Size: 40 812 408 612 652 581 199 302
Cuts at: 13998 14103 15192 15235 15667 15723 15874 16763 18392
Size: 105 1089 43 432 56 151 889 1629
Cuts at: 18392 8
Size: 43
Fragments arranged by size:
1629 1425 1227 1182 1089 1002 889 812
652 624 612 581 472 432 422 408
405 357 329 303 302 294 275 260
238 228 199 189 183 174 151 144
105 91 78 76 75 60 60 56
54 50 43 43 40 39 33 18
17
EagI C'GGCC_G
Cuts at: 1537 1812 2653 2982 7465 7896 8070 10392 12916
Size: 275 841 329 4483 431 174 2322 2524
Cuts at: 12916 14103 15192 15667 15874 1537
Size: 1187 1089 475 207 4090
Fragments arranged by size:
4483 4090 2524 2322 1187 1089 841 475
431 329 275 207 174
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3446 4751 5013 7043 7512 8054 8229
Size: 1313 780 1305 262 2030 469 542 175
Cuts at: 8229 8415 8780 9647 10454 12431 12783 13436 14165
Size: 186 365 867 807 1977 352 653 729
Cuts at: 14165 14303 15643 16357 1353
Size: 138 1340 714 3423
Fragments arranged by size:
3423 2030 1977 1340 1313 1305 867 807
780 729 714 653 542 469 365 352
262 186 175 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2777 3434 4336 6257 6392 6545
Size: 1064 138 1188 657 902 1921 135 153
Cuts at: 6545 6554 6638 6734 6743 6827 7043 7106 7136
Size: 9 84 96 9 84 216 63 30
Cuts at: 7136 7202 7529 7592 7655 7682 7709 7925 8030
Size: 66 327 63 63 27 27 216 105
Cuts at: 8030 8246 8348 8378 8399 8555 8741 8930 9086
Size: 216 102 30 21 156 186 189 156
Cuts at: 9086 9173 9329 10051 10484 10631 12461 12608 12737
Size: 87 156 722 433 147 1830 147 129
Cuts at: 12737 12971 13117 13136 13949 14195 17014 17388 17534
Size: 234 146 19 813 246 2819 374 146
Cuts at: 17534 387
Size: 1280
Fragments arranged by size:
2819 1921 1830 1280 1188 1064 902 813
722 657 433 374 327 246 234 216
216 216 189 186 156 156 156 153
147 147 146 146 138 135 129 105
102 96 87 84 84 66 63 63
63 30 30 27 27 21 19 9
9
EcoICRI GAG'CTC
Cuts at: 4108 5050 5458 5689 6369 8773 9349 10129 11071
Size: 942 408 231 680 2404 576 780 942
Cuts at: 11071 11668 11755 11851 12034 13981 4108
Size: 597 87 96 183 1947 8554
Fragments arranged by size:
8554 2404 1947 942 942 780 680 597
576 408 231 183 96 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2345 2623 2665 2753 2945 2995
Size: 393 429 284 278 42 88 192 50
Cuts at: 2995 3071 3143 3239 3251 3278 3581 3656 3950
Size: 76 72 96 12 27 303 75 294
Cuts at: 3950 4036 4100 4106 4259 4385 4487 4721 4856
Size: 86 64 6 153 126 102 234 135
Cuts at: 4856 4892 5093 5306 5327 5360 5501 5870 5921
Size: 36 201 213 21 33 141 369 51
Cuts at: 5921 6212 6719 6806 6881 6998 7187 7301 7472
Size: 291 507 87 75 117 189 114 171
Cuts at: 7472 7745 7823 7895 7937 7943 8006 8129 8456
Size: 273 78 72 42 6 63 123 327
Cuts at: 8456 8690 8741 9209 9233 9407 9470 9653 9833
Size: 234 51 468 24 174 63 183 180
Cuts at: 9833 9908 10214 10295 10523 10709 11066 11198 11237
Size: 75 306 81 228 186 357 132 39
Cuts at: 11237 11465 11654 11983 12191 12272 12500 12550 12686
Size: 228 189 329 208 81 228 50 136
Cuts at: 12686 13277 13565 14006 14054 14210 14234 14468 14612
Size: 591 288 441 48 156 24 234 144
Cuts at: 14612 14857 14972 15041 15227 15269 15497 16490 17087
Size: 245 115 69 186 42 228 993 597
Cuts at: 17087 17874 18142 1239
Size: 787 268 1524
Fragments arranged by size:
1524 993 787 597 591 507 468 441
429 393 369 357 329 327 306 303
294 291 288 284 278 273 268 245
234 234 234 228 228 228 228 213
208 201 192 189 189 186 186 183
180 174 171 156 153 144 141 136
135 132 126 123 117 115 114 102
96 88 87 86 81 81 78 76
75 75 75 72 72 69 64 63
63 51 51 50 50 48 42 42
42 39 36 33 27 24 24 21
12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2324 2744 4376 5003 5849 6743 6875 7058
Size: 69 420 1632 627 846 894 132 183
Cuts at: 7058 8885 9431 10274 10502 11435 11447 11576 11981
Size: 1827 546 843 228 933 12 129 405
Cuts at: 11981 12251 12428 12479 12848 13043 13106 13574 14162
Size: 270 177 51 369 195 63 468 588
Cuts at: 14162 14213 15209 15311 2255
Size: 51 996 102 5371
Fragments arranged by size:
5371 1827 1632 996 933 894 846 843
627 588 546 468 420 405 369 270
228 195 183 177 132 129 102 69
63 51 51 12
EcoO109I rG'GnC_Cy
Cuts at: 2329 2614 2770 3664 3924 4369 4580 4717 5403
Size: 285 156 894 260 445 211 137 686
Cuts at: 5403 5842 6685 6694 8983 9553 10364 10420 10468
Size: 439 843 9 2289 570 811 56 48
Cuts at: 10468 10705 10903 11011 11348 11404 11440 11452 11473
Size: 237 198 108 337 56 36 12 21
Cuts at: 11473 11592 11593 11806 12049 12103 12217 12341 12445
Size: 119 1 213 243 54 114 124 104
Cuts at: 12445 12682 13036 13336 13346 13471 13825 14075 14131
Size: 237 354 300 10 125 354 250 56
Cuts at: 14131 14179 14566 14877 14918 15100 15166 15202 15316
Size: 48 387 311 41 182 66 36 114
Cuts at: 15316 15687 15706 15707 2329
Size: 371 19 1 5049
Fragments arranged by size:
5049 2289 894 843 811 686 570 445
439 387 371 354 354 337 311 300
285 260 250 243 237 237 213 211
198 182 156 137 125 124 119 114
114 108 104 66 56 56 56 54
48 48 41 36 36 21 19 12
10 9 1 1
EcoRI G'AATT_C
Cuts at: 3785 15736 16114 3785
Size: 11951 378 6098
Fragments arranged by size:
11951 6098 378
EcoRV GAT'ATC
Cuts at: 2530 2530
Size: 18427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 7900 10660 12637 13753 14371 2242
Size: 5658 2760 1977 1116 618 6298
Fragments arranged by size:
6298 5658 2760 1977 1116 618
FseI GG_CCGG'CC
Cuts at: 7467 12918 15237 7467
Size: 5451 2319 10657
Fragments arranged by size:
10657 5451 2319
FspI TGC'GCA
Cuts at: 6431 7781 7964 8501 9102 11018 13616 14495 16933
Size: 1350 183 537 601 1916 2598 879 2438
Cuts at: 16933 6431
Size: 7925
Fragments arranged by size:
7925 2598 2438 1916 1350 879 601 537
183
HaeII r_GCGC'y
Cuts at: 1678 2142 3526 3628 3711 4067 4855 5038 5614
Size: 464 1384 102 83 356 788 183 576
Cuts at: 5614 5647 5748 5866 6484 6580 6739 6868 6985
Size: 33 101 118 618 96 159 129 117
Cuts at: 6985 7010 7210 7681 7768 7874 8035 8108 8155
Size: 25 200 471 87 106 161 73 47
Cuts at: 8155 8878 9225 9238 9301 9427 9607 10696 11101
Size: 723 347 13 63 126 180 1089 405
Cuts at: 11101 11307 12304 13648 13811 14503 14689 15073 15244
Size: 206 997 1344 163 692 186 384 171
Cuts at: 15244 15375 17574 1678
Size: 131 2199 2531
Fragments arranged by size:
2531 2199 1384 1344 1089 997 788 723
692 618 576 471 464 405 384 356
347 206 200 186 183 180 171 163
161 159 131 129 126 118 117 106
102 101 96 87 83 73 63 47
33 25 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2816 2848 7400 7432 8162
Size: 32 135 32 1244 32 4552 32 730
Cuts at: 8162 8194 11978 12010 12176 12208 12389 12421 13045
Size: 32 3784 32 166 32 181 32 624
Cuts at: 13045 13077 13109 13141 13580 13612 14123 14155 14496
Size: 32 32 32 439 32 511 32 341
Cuts at: 14496 14528 14663 14695 14777 14809 14945 14977 17068
Size: 32 135 32 82 32 136 32 2091
Cuts at: 17068 17100 17142 17174 1373
Size: 32 42 32 2626
Fragments arranged by size:
4552 3784 2626 2091 1244 730 624 511
439 341 181 166 136 135 135 82
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 10336 15094 15771 16017 669
Size: 988 281 304 8094 4758 677 246 3079
Fragments arranged by size:
8094 4758 3079 988 677 304 281 246
HindIII A'AGCT_T
Cuts at: 748 9565 748
Size: 8817 9610
HpaI GTT'AAC
Cuts at: 16017 16017
Size: 18427
KasI G'GCGC_C
Cuts at: 3624 5744 6735 8031 8151 14685 15371 3624
Size: 2120 991 1296 120 6534 686 6680
Fragments arranged by size:
6680 6534 2120 1296 991 686 120
KpnI G_GTAC'C
Cuts at: 2611 15752 2611
Size: 13141 5286
MfeI C'AATT_G
Cuts at: 16026 16026
Size: 18427
MluI A'CGCG_T
Cuts at: 18303 18303
Size: 18427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2529 3327 6447 9496 11238 11262 11466 12843
Size: 1224 798 3120 3049 1742 24 204 1377
Cuts at: 12843 13698 17541 17725 1305
Size: 855 3843 184 2007
Fragments arranged by size:
3843 3120 3049 2007 1742 1377 1224 855
798 204 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2304 3212 4439 4820 7358
Size: 54 1425 343 472 908 1227 381 2538
Cuts at: 7358 7391 9659 9734 10434 13499 10
Size: 33 2268 75 700 3065 4938
Fragments arranged by size:
4938 3065 2538 2268 1425 1227 908 700
472 381 343 75 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 2702 4034 4557 5137 10118 10967
Size: 648 1108 428 1332 523 580 4981 849
Cuts at: 10967 10970 11246 12752 16385 16744 16903 518
Size: 3 276 1506 3633 359 159 2042
Fragments arranged by size:
4981 3633 2042 1506 1332 1108 849 648
580 523 428 359 276 159 3
MspA1I CmG'CkG
Cuts at: 1606 2116 2130 2171 2643 2920 3175 3319 3385
Size: 510 14 41 472 277 255 144 66
Cuts at: 3385 3748 4213 4405 4465 4573 4987 5071 5116
Size: 363 465 192 60 108 414 84 45
Cuts at: 5116 5373 5581 5635 5710 5797 5827 5941 6148
Size: 257 208 54 75 87 30 114 207
Cuts at: 6148 6202 6208 6358 6682 6859 6901 7144 7177
Size: 54 6 150 324 177 42 243 33
Cuts at: 7177 7183 7217 7270 7276 7534 7540 7567 7714
Size: 6 34 53 6 258 6 27 147
Cuts at: 7714 7777 7843 7901 8002 8008 8095 8191 8197
Size: 63 66 58 101 6 87 96 6
Cuts at: 8197 8320 8573 8668 8716 8971 8998 9154 9181
Size: 123 253 95 48 255 27 156 27
Cuts at: 9181 9205 9211 9535 9598 9673 9691 9724 9811
Size: 24 6 324 63 75 18 33 87
Cuts at: 9811 9823 9877 9995 10096 10243 10897 11083 11497
Size: 12 54 118 101 147 654 186 414
Cuts at: 11497 11677 11695 11884 12003 12361 12661 12886 12895
Size: 180 18 189 119 358 300 225 9
Cuts at: 12895 13495 13594 13663 13702 13816 13884 14395 14446
Size: 600 99 69 39 114 68 511 51
Cuts at: 14446 14884 15013 15528 15545 15594 15843 16520 17668
Size: 438 129 515 17 49 249 677 1148
Cuts at: 17668 17913 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 654 600 515 511 510
472 465 438 414 414 363 358 324
324 300 277 258 257 255 255 253
249 245 243 225 208 207 192 189
186 180 177 156 150 147 147 144
129 123 119 118 114 114 108 101
101 99 96 95 87 87 87 84
75 75 69 68 66 66 63 63
60 58 54 54 54 53 51 49
48 45 42 41 39 34 33 33
30 27 27 27 24 18 18 17
14 12 9 6 6 6 6 6
6 6
NaeI GCC'GGC
Cuts at: 2123 5130 5442 6425 7465 10069 10997 11272 11652
Size: 3007 312 983 1040 2604 928 275 380
Cuts at: 11652 11684 12160 12916 14758 15235 15583 2123
Size: 32 476 756 1842 477 348 4967
Fragments arranged by size:
4967 3007 2604 1842 1040 983 928 756
477 476 380 348 312 275 32
NarI GG'CG_CC
Cuts at: 3625 5745 6736 8032 8152 14686 15372 3625
Size: 2120 991 1296 120 6534 686 6680
Fragments arranged by size:
6680 6534 2120 1296 991 686 120
NcoI C'CATG_G
Cuts at: 513 1065 13764 14197 513
Size: 552 12699 433 4743
Fragments arranged by size:
12699 4743 552 433
NdeI CA'TA_TG
Cuts at: 387 387
Size: 18427
NgoMIV G'CCGG_C
Cuts at: 2121 5128 5440 6423 7463 10067 10995 11270 11650
Size: 3007 312 983 1040 2604 928 275 380
Cuts at: 11650 11682 12158 12914 14756 15233 15581 2121
Size: 32 476 756 1842 477 348 4967
Fragments arranged by size:
4967 3007 2604 1842 1040 983 928 756
477 476 380 348 312 275 32
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 18427
NotI GC'GGCC_GC
Cuts at: 7896 15874 7896
Size: 7978 10449
NspI r_CATG'y
Cuts at: 1132 1229 6277 14791 15785 17330 1132
Size: 97 5048 8514 994 1545 2229
Fragments arranged by size:
8514 5048 2229 1545 994 97
PasI CC'CwG_GG
Cuts at: 1348 2078 15700 1348
Size: 730 13622 4075
Fragments arranged by size:
13622 4075 730
PciI A'CATG_T
Cuts at: 17326 17326
Size: 18427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3121 3175 4576 4720 12898 1108
Size: 2013 54 1401 144 8178 6637
Fragments arranged by size:
8178 6637 2013 1401 144 54
PfoI T'CCnGG_A
Cuts at: 2160 2790 3216 5451 6024 9645 9885 10479 13083
Size: 630 426 2235 573 3621 240 594 2604
Cuts at: 13083 13869 13983 14873 14934 15246 2160
Size: 786 114 890 61 312 5341
Fragments arranged by size:
5341 3621 2604 2235 890 786 630 594
573 426 312 240 114 61
PmeI GTTT'AAAC
Cuts at: 15731 15731
Size: 18427
PmlI CAC'GTG
Cuts at: 3208 4747 6886 3208
Size: 1539 2139 14749
Fragments arranged by size:
14749 2139 1539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 13025 13057 16496 16528 18009 18041 13025
Size: 32 3439 32 1481 32 13411
Fragments arranged by size:
13411 3439 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2329 2770 3664 3924 4369 8983 10420 10705 10903
Size: 441 894 260 445 4614 1437 285 198
Cuts at: 10903 11404 11806 12049 12103 12682 13036 14131 14877
Size: 501 402 243 54 579 354 1095 746
Cuts at: 14877 15166 15687 2329
Size: 289 521 5069
Fragments arranged by size:
5069 4614 1437 1095 894 746 579 521
501 445 441 402 354 289 285 260
243 198 54
PshAI GACnn'nnGTC
Cuts at: 1613 12095 1613
Size: 10482 7945
PsiI TTA'TAA
Cuts at: 15997 15997
Size: 18427
PspOMI G'GGCC_C
Cuts at: 4579 5403 5493 6684 10363 11347 11592 11739 12340
Size: 824 90 1191 3679 984 245 147 601
Cuts at: 12340 12888 13346 14074 14917 15706 4579
Size: 548 458 728 843 789 7300
Fragments arranged by size:
7300 3679 1191 984 843 824 789 728
601 548 458 245 147 90
PspXI vC'TCGA_Gb
Cuts at: 5459 5690 5459
Size: 231 18196
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2699 2731 4238 4270 6074 6106 10658 10690 12635
Size: 32 1507 32 1804 32 4552 32 1945
Cuts at: 12635 12667 14369 14401 2699
Size: 32 1702 32 6725
Fragments arranged by size:
6725 4552 1945 1804 1702 1507 32 32
32 32 32 32
PstI C_TGCA'G
Cuts at: 830 2388 2919 3291 3345 3370 3612 3663 3885
Size: 1558 531 372 54 25 242 51 222
Cuts at: 3885 4188 4311 4380 4970 4995 5070 5577 5757
Size: 303 123 69 590 25 75 507 180
Cuts at: 5757 5832 6267 6354 6465 6513 6693 6717 6822
Size: 75 435 87 111 48 180 24 105
Cuts at: 6822 6876 7026 7305 7321 8196 8229 8295 8491
Size: 54 150 279 16 875 33 66 196
Cuts at: 8491 8667 8715 8886 9084 9126 9495 9543 9561
Size: 176 48 171 198 42 369 48 18
Cuts at: 9561 9807 9882 10062 10095 10164 10236 10683 11088
Size: 246 75 180 33 69 72 447 405
Cuts at: 11088 11772 12141 13815 14394 15324 15779 830
Size: 684 369 1674 579 930 455 3478
Fragments arranged by size:
3478 1674 1558 930 875 684 590 579
531 507 455 447 435 405 372 369
369 303 279 246 242 222 198 196
180 180 180 176 171 150 123 111
105 87 75 75 75 72 69 69
66 54 54 51 48 48 48 42
33 33 25 25 24 18 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 18427
PvuII CAG'CTG
Cuts at: 2171 2643 2920 3319 3748 4213 4405 4465 4573
Size: 472 277 399 429 465 192 60 108
Cuts at: 4573 4987 5071 5635 5710 5827 5941 6208 6358
Size: 414 84 564 75 117 114 267 150
Cuts at: 6358 6859 6901 7183 7276 7540 7777 8008 8191
Size: 501 42 282 93 264 237 231 183
Cuts at: 8191 8197 8320 8573 8668 8971 9154 9211 9535
Size: 6 123 253 95 303 183 57 324
Cuts at: 9535 9673 9811 9877 10243 11083 11497 11695 12361
Size: 138 138 66 366 840 414 198 666
Cuts at: 12361 12661 12895 13495 13594 13663 13702 13816 13884
Size: 300 234 600 99 69 39 114 68
Cuts at: 13884 14395 14884 2171
Size: 511 489 5714
Fragments arranged by size:
5714 840 666 600 564 511 501 489
472 465 429 414 414 399 366 324
303 300 282 277 267 264 253 237
234 231 198 192 183 183 150 138
138 123 117 114 114 108 99 95
93 84 75 69 68 66 60 57
42 39 6
SacI G_AGCT'C
Cuts at: 4110 5052 5460 5691 6371 8775 9351 10131 11073
Size: 942 408 231 680 2404 576 780 942
Cuts at: 11073 11670 11757 11853 12036 13983 4110
Size: 597 87 96 183 1947 8554
Fragments arranged by size:
8554 2404 1947 942 942 780 680 597
576 408 231 183 96 87
SacII CC_GC'GG
Cuts at: 5374 7218 9996 15595 5374
Size: 1844 2778 5599 8206
Fragments arranged by size:
8206 5599 2778 1844
SalI G'TCGA_C
Cuts at: 1936 15769 1936
Size: 13833 4594
SanDI GG'GwC_CC
Cuts at: 11404 11404
Size: 18427
SapI GCTCTTCn'nnn_
Cuts at: 3446 4751 7043 8054 8780 9647 13436 3446
Size: 1305 2292 1011 726 867 3789 8437
Fragments arranged by size:
8437 3789 2292 1305 1011 867 726
SbfI CC_TGCA'GG
Cuts at: 3663 6693 6876 8886 10164 15779 3663
Size: 3030 183 2010 1278 5615 6311
Fragments arranged by size:
6311 5615 3030 2010 1278 183
ScaI AGT'ACT
Cuts at: 11867 16675 18387 11867
Size: 4808 1712 11907
Fragments arranged by size:
11907 4808 1712
SexAI A'CCwGG_T
Cuts at: 14988 14988
Size: 18427
SfcI C'TryA_G
Cuts at: 826 1045 2384 2915 3287 3341 3366 3608 3659
Size: 219 1339 531 372 54 25 242 51
Cuts at: 3659 3881 4184 4208 4307 4376 4966 4991 5066
Size: 222 303 24 99 69 590 25 75
Cuts at: 5066 5573 5753 5828 6052 6263 6350 6461 6509
Size: 507 180 75 224 211 87 111 48
Cuts at: 6509 6689 6713 6818 6872 7022 7301 7317 8192
Size: 180 24 105 54 150 279 16 875
Cuts at: 8192 8225 8279 8291 8487 8663 8711 8882 9080
Size: 33 54 12 196 176 48 171 198
Cuts at: 9080 9122 9491 9539 9557 9593 9803 9878 10058
Size: 42 369 48 18 36 210 75 180
Cuts at: 10058 10091 10160 10232 10679 11084 11626 11768 12137
Size: 33 69 72 447 405 542 142 369
Cuts at: 12137 12391 13811 14390 15320 15508 15517 15775 16910
Size: 254 1420 579 930 188 9 258 1135
Cuts at: 16910 17591 17782 826
Size: 681 191 1471
Fragments arranged by size:
1471 1420 1339 1135 930 875 681 590
579 542 531 507 447 405 372 369
369 303 279 258 254 242 224 222
219 211 210 198 196 191 188 180
180 180 176 171 150 142 111 105
99 87 75 75 75 72 69 69
54 54 54 51 48 48 48 42
36 33 33 25 25 24 24 18
16 12 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4445 4685 5441 5471 5981 6692 7490 8207 8738
Size: 240 756 30 510 711 798 717 531
Cuts at: 8738 11346 15146 15200 4445
Size: 2608 3800 54 7672
Fragments arranged by size:
7672 3800 2608 798 756 717 711 531
510 240 54 30
SfoI GGC'GCC
Cuts at: 3626 5746 6737 8033 8153 14687 15373 3626
Size: 2120 991 1296 120 6534 686 6680
Fragments arranged by size:
6680 6534 2120 1296 991 686 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 18427
SgrAI Cr'CCGG_yG
Cuts at: 1700 13502 14804 15062 1700
Size: 11802 1302 258 5065
Fragments arranged by size:
11802 5065 1302 258
SmaI CCC'GGG
Cuts at: 1872 2354 3845 8375 8468 10790 11465 15578 15754
Size: 482 1491 4530 93 2322 675 4113 176
Cuts at: 15754 1872
Size: 4545
Fragments arranged by size:
4545 4530 4113 2322 1491 675 482 176
93
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3149 3461 5348 5420 5459 5639
Size: 197 926 1206 312 1887 72 39 180
Cuts at: 5639 5690 5963 6992 7628 7652 8813 9020 9059
Size: 51 273 1029 636 24 1161 207 39
Cuts at: 9059 9239 9497 12077 13064 13115 13214 15410 16531
Size: 180 258 2580 987 51 99 2196 1121
Cuts at: 16531 17432 17694 820
Size: 901 262 1553
Fragments arranged by size:
2580 2196 1887 1553 1206 1161 1121 1029
987 926 901 636 312 273 262 258
207 197 180 180 99 72 51 51
39 39 24
SnaBI TAC'GTA
Cuts at: 493 16218 493
Size: 15725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 18427
SphI G_CATG'C
Cuts at: 1132 6277 15785 1132
Size: 5145 9508 3774
Fragments arranged by size:
9508 5145 3774
SrfI GCCC'GGGC
Cuts at: 8468 8468
Size: 18427
SspI AAT'ATT
Cuts at: 5 52 16351 5
Size: 47 16299 2081
Fragments arranged by size:
16299 2081 47
StuI AGG'CCT
Cuts at: 1517 2750 3765 4434 4889 5078 5474 7706 11624
Size: 1233 1015 669 455 189 396 2232 3918
Cuts at: 11624 14555 15032 15149 1517
Size: 2931 477 117 4795
Fragments arranged by size:
4795 3918 2931 2232 1233 1015 669 477
455 396 189 117
StyI C'CwwG_G
Cuts at: 513 1065 2101 2485 2565 3078 3102 3909 4449
Size: 552 1036 384 80 513 24 807 540
Cuts at: 4449 5469 5739 5994 6390 7359 8133 9003 9660
Size: 1020 270 255 396 969 774 870 657
Cuts at: 9660 10635 10752 11606 11619 12180 12612 12927 13764
Size: 975 117 854 13 561 432 315 837
Cuts at: 13764 13914 14197 14346 15498 15866 513
Size: 150 283 149 1152 368 3074
Fragments arranged by size:
3074 1152 1036 1020 975 969 870 854
837 807 774 657 561 552 540 513
432 396 384 368 315 283 270 255
150 149 117 80 24 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3667 4494 5684 8693 9371 11804 14342
Size: 1255 2087 827 1190 3009 678 2433 2538
Cuts at: 14342 16454 16471 16624 16809 17254 325
Size: 2112 17 153 185 445 1498
Fragments arranged by size:
3009 2538 2433 2112 2087 1498 1255 1190
827 678 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3546 4413 5907
Size: 274 80 33 51 1443 1569 867 1494
Cuts at: 5907 6081 11865 12756 16673 18385 96
Size: 174 5784 891 3917 1712 138
Fragments arranged by size:
5784 3917 1712 1569 1494 1443 891 867
274 174 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3176 3208 3959 3991 9375 9407 12815 12847 13090
Size: 32 751 32 5384 32 3408 32 243
Cuts at: 13090 13122 14600 14632 18387 18419 3176
Size: 32 1478 32 3755 32 3184
Fragments arranged by size:
5384 3755 3408 3184 1478 751 243 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 15089 1316
Size: 13773 4654
XbaI T'CTAG_A
Cuts at: 15763 15763
Size: 18427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3289 7447 9445 12034 12274 13771 14881 3289
Size: 4158 1998 2589 240 1497 1110 6835
Fragments arranged by size:
6835 4158 2589 1998 1497 1110 240
XhoI C'TCGA_G
Cuts at: 1943 5459 5690 13064 13115 1943
Size: 3516 231 7374 51 7255
Fragments arranged by size:
7374 7255 3516 231 51
XmaI C'CCGG_G
Cuts at: 1870 2352 3843 8373 8466 10788 11463 15576 15752
Size: 482 1491 4530 93 2322 675 4113 176
Cuts at: 15752 1870
Size: 4545
Fragments arranged by size:
4545 4530 4113 2322 1491 675 482 176
93
XmnI GAAnn'nnTTC
Cuts at: 2225 15149 16556 2225
Size: 12924 1407 4096
Fragments arranged by size:
12924 4096 1407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10795 14475 16235 276
Size: 53 83 186 936 9261 3680 1760 2468
Fragments arranged by size:
9261 3680 2468 1760 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BstZ17I FspAI NruI NsiI
PacI RsrII SwaI