Restriction map for
pFN21AE2486
(Circular) (Six-base) MAPSORT of: pFN21AE2486.seq Check: 5421 from: 1 to: 11374
pFN21AE2486 11374 bp
With 182 enzymes: SgfI *
November 15, 2013 16:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2904 5319 5412 9184
Size: 53 83 186 936 1368 2415 93 3772
Cuts at: 9184 278
Size: 2468
Fragments arranged by size:
3772 2468 2415 1368 936 186 93 83
53
AccI GT'mk_AC
Cuts at: 1937 2904 5454 8717 1937
Size: 967 2550 3263 4594
Fragments arranged by size:
4594 3263 2550 967
Acc65I G'GTAC_C
Cuts at: 8695 8695
Size: 11374
AclI AA'CG_TT
Cuts at: 1563 9501 9874 1563
Size: 7938 373 3063
Fragments arranged by size:
7938 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 4091 4315 5900 6354 6632 6812 8212
Size: 863 1596 224 1585 454 278 180 1400
Cuts at: 8212 9437 10821 11089 1632
Size: 1225 1384 268 1917
Fragments arranged by size:
1917 1596 1585 1400 1384 1225 863 454
278 268 224 180
AfeI AGC'GCT
Cuts at: 3257 4898 3257
Size: 1641 9733
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11177
AflIII A'CryG_T
Cuts at: 1203 6276 8046 10273 11250 1203
Size: 5073 1770 2227 977 1327
Fragments arranged by size:
5073 2227 1770 1327 977
AgeI A'CCGG_T
Cuts at: 10205 10205
Size: 11374
AhdI GACnn_n'nnGTC
Cuts at: 2326 3480 7474 10103 2326
Size: 1154 3994 2629 3597
Fragments arranged by size:
3994 3597 2629 1154
AleI CACnn'nnGTG
Cuts at: 1166 3009 3617 6839 1166
Size: 1843 608 3222 5701
Fragments arranged by size:
5701 3222 1843 608
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 3555 4574 5948 1184
Size: 1179 1192 1019 1374 6610
Fragments arranged by size:
6610 1374 1192 1179 1019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4403 4435 4403
Size: 32 11342
AlwNI CAG_nnn'CTG
Cuts at: 2054 3382 3403 4152 5721 6112 6403 7913 9117
Size: 1328 21 749 1569 391 291 1510 1204
Cuts at: 9117 10689 2054
Size: 1572 2739
Fragments arranged by size:
2739 1572 1569 1510 1328 1204 749 391
291 21
ApaI G_GGCC'C
Cuts at: 3410 3441 3787 5136 6826 7283 8603 3410
Size: 31 346 1349 1690 457 1320 6181
Fragments arranged by size:
6181 1690 1349 1320 457 346 31
ApaLI G'TGCA_C
Cuts at: 6454 9431 10587 6454
Size: 2977 1156 7241
Fragments arranged by size:
7241 2977 1156
ApoI r'AATT_y
Cuts at: 1493 8683 8909 9061 11170 1493
Size: 7190 226 152 2109 1697
Fragments arranged by size:
7190 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9928 160
Size: 872 8896 1606
Fragments arranged by size:
8896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11374
AvaI C'yCGr_G
Cuts at: 1870 1943 2441 3562 3697 3718 3733 5049 5238
Size: 73 498 1121 135 21 15 1316 189
Cuts at: 5238 5270 5468 6783 6911 7253 7288 7309 7625
Size: 32 198 1315 128 342 35 21 316
Cuts at: 7625 8099 8169 8287 8699 1870
Size: 474 70 118 412 4545
Fragments arranged by size:
4545 1316 1315 1121 498 474 412 342
316 198 189 135 128 118 73 70
35 32 21 21 15
AvrII C'CTAG_G
Cuts at: 4774 4774
Size: 11374
BamHI G'GATC_C
Cuts at: 2592 7753 8704 9071 2592
Size: 5161 951 367 4895
Fragments arranged by size:
5161 4895 951 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 2922 3011 3393 4251
Size: 325 217 627 380 755 89 382 858
Cuts at: 4251 4396 4740 4770 5045 5215 5228 5869 6369
Size: 145 344 30 275 170 13 641 500
Cuts at: 6369 7313 8578 8608 8695 10150 618
Size: 944 1265 30 87 1455 1842
Fragments arranged by size:
1842 1455 1265 944 858 755 641 627
500 382 380 344 325 275 217 170
145 89 87 30 30 13
BanII G_rGCy'C
Cuts at: 1391 2096 3161 3410 3441 3456 3734 3740 3749
Size: 705 1065 249 31 15 278 6 9
Cuts at: 3749 3787 4092 4649 4850 4922 5136 6826 6924
Size: 38 305 557 201 72 214 1690 98
Cuts at: 6924 7254 7283 7484 7692 7982 8279 8537 8603
Size: 330 29 201 208 290 297 258 66
Cuts at: 8603 8693 1391
Size: 90 4072
Fragments arranged by size:
4072 1690 1065 705 557 330 305 297
290 278 258 249 214 208 201 201
98 90 72 66 38 31 29 15
9 6
BbeI G_GCGC'C
Cuts at: 2171 2926 3397 4255 5049 5232 5873 6373 7317
Size: 755 471 858 794 183 641 500 944
Cuts at: 7317 8612 2171
Size: 1295 4933
Fragments arranged by size:
4933 1295 944 858 794 755 641 500
471 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2653 3479 4030 4280 5311 5448 5740
Size: 971 754 826 551 250 1031 137 292
Cuts at: 5740 928
Size: 6562
Fragments arranged by size:
6562 1031 971 826 754 551 292 250
137
BbvCI CC'TCA_GC
Cuts at: 5785 8384 5785
Size: 2599 8775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3961 3995 5386 5420 5545 5579 6707
Size: 34 2024 34 1391 34 125 34 1128
Cuts at: 6707 6741 7226 7260 9565 9599 1903
Size: 34 485 34 2305 34 3678
Fragments arranged by size:
3678 2305 2024 1391 1128 485 125 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9266 10476 9266
Size: 1210 10164
BclI T'GATC_A
Cuts at: 1548 3063 3171 3556 1548
Size: 1515 108 385 9366
Fragments arranged by size:
9366 1515 385 108
BfrBI ATG'CAT
Cuts at: 4348 4348
Size: 11374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2988 3641 3729
Size: 107 122 71 631 605 1316 653 88
Cuts at: 3729 5252 7650 7926 8214 8340 8513 9985 136
Size: 1523 2398 276 288 126 173 1472 1525
Fragments arranged by size:
2398 1525 1523 1472 1316 653 631 605
288 276 173 126 122 107 88 71
BglII A'GATC_T
Cuts at: 3315 4023 5802 11369 3315
Size: 708 1779 5567 3320
Fragments arranged by size:
5567 3320 1779 708
BlpI GC'TnA_GC
Cuts at: 2055 8791 2055
Size: 6736 4638
Bme1580I G_kGCm'C
Cuts at: 1790 2583 3410 3441 3581 3787 4401 4743 4773
Size: 793 827 31 140 206 614 342 30
Cuts at: 4773 5136 5220 6458 6826 7283 7943 8140 8581
Size: 363 84 1238 368 457 660 197 441
Cuts at: 8581 8603 9435 10591 1790
Size: 22 832 1156 2573
Fragments arranged by size:
2573 1238 1156 832 827 793 660 614
457 441 368 363 342 206 197 140
84 31 30 22
BmgBI CAC'GTC
Cuts at: 1318 6543 8049 1318
Size: 5225 1506 4643
Fragments arranged by size:
5225 4643 1506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2577 3196 3827
Size: 416 53 83 399 24 1160 619 631
Cuts at: 3827 4746 4772 9105 10063 442
Size: 919 26 4333 958 1753
Fragments arranged by size:
4333 1753 1160 958 919 631 619 416
399 83 53 26 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 5999 7588 2035
Size: 3964 1589 5821
Fragments arranged by size:
5821 3964 1589
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2747 3253 3263 4040 4079 4407 4661
Size: 899 609 506 10 777 39 328 254
Cuts at: 4661 4954 5269 5591 6395 6542 6569 6620 6728
Size: 293 315 322 804 147 27 51 108
Cuts at: 6728 7280 7849 8145 8660 10034 1239
Size: 552 569 296 515 1374 2579
Fragments arranged by size:
2579 1374 899 804 777 609 569 552
515 506 328 322 315 296 293 254
147 108 51 39 27 10
Bpu10I CC'TnA_GC
Cuts at: 5785 7100 7686 8117 8204 8384 5785
Size: 1315 586 431 87 180 8775
Fragments arranged by size:
8775 1315 586 431 180 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 2846 4970 5897 6545 9499 10364 10662 2384
Size: 462 2124 927 648 2954 865 298 3096
Fragments arranged by size:
3096 2954 2124 927 865 648 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3206 7030 7698 8372 10037 882
Size: 617 1707 3824 668 674 1665 2219
Fragments arranged by size:
3824 2219 1707 1665 674 668 617
BsaAI yAC'GTr
Cuts at: 493 1204 3100 9165 493
Size: 711 1896 6065 2702
Fragments arranged by size:
6065 2702 1896 711
BsaBI GATnn'nnATC
Cuts at: 3050 3950 6719 11368 3050
Size: 900 2769 4649 3056
Fragments arranged by size:
4649 3056 2769 900
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 2901 2923 3394
Size: 53 83 186 936 635 733 22 471
Cuts at: 3394 3605 4252 4614 5046 5229 5316 5409 5870
Size: 211 647 362 432 183 87 93 461
Cuts at: 5870 6370 7233 7314 8058 8609 8652 9181 9563
Size: 500 863 81 744 551 43 529 382
Cuts at: 9563 10198 275
Size: 635 1451
Fragments arranged by size:
1451 936 863 744 733 647 635 635
551 529 500 471 461 432 382 362
211 186 183 93 87 83 81 53
43 22
BsaWI w'CCGG_w
Cuts at: 7803 9807 10205 10479 10626 11037 7803
Size: 2004 398 274 147 411 8140
Fragments arranged by size:
8140 2004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3077 3107 3251 3281 3791
Size: 30 165 30 1674 30 144 30 510
Cuts at: 3791 3821 4380 4403 4410 4433 4883 4913 5000
Size: 30 559 23 7 23 450 30 87
Cuts at: 5000 5030 6224 6254 7391 7421 7484 7514 8291
Size: 30 1194 30 1137 30 63 30 777
Cuts at: 8291 8321 1178
Size: 30 4231
Fragments arranged by size:
4231 1674 1194 1137 777 559 510 450
165 144 87 63 30 30 30 30
30 30 30 30 30 30 30 23
23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 2135 2744 2798 3326 3799 3842 3845
Size: 834 115 609 54 528 473 43 3
Cuts at: 3845 3848 3851 3854 4016 5375 5896 6368 6725
Size: 3 3 3 162 1359 521 472 357
Cuts at: 6725 6866 7492 7796 8012 8342 1186
Size: 141 626 304 216 330 4218
Fragments arranged by size:
4218 1359 834 626 609 528 521 473
472 357 330 304 216 162 141 115
54 43 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2047 2629 3078 4784 5300 5680 6069 6396 7606
Size: 582 449 1706 516 380 389 327 1210
Cuts at: 7606 8066 10577 11073 2047
Size: 460 2511 496 2348
Fragments arranged by size:
2511 2348 1706 1210 582 516 496 460
449 389 380 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2708 3641 5084 5999 7060 7568 8278
Size: 573 972 933 1443 915 1061 508 710
Cuts at: 8278 1163
Size: 4259
Fragments arranged by size:
4259 1443 1061 972 933 915 710 573
508
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2335 3688 5338 7743 8218 8494
Size: 275 186 334 1353 1650 2405 475 276
Cuts at: 8494 8575 8824 9585 10613 1540
Size: 81 249 761 1028 2301
Fragments arranged by size:
2405 2301 1650 1353 1028 761 475 334
276 275 249 186 81
BsiHKAI G_wGCw'C
Cuts at: 2400 3231 3749 3987 4269 4563 4649 4922 5937
Size: 831 518 238 282 294 86 273 1015
Cuts at: 5937 6273 6458 6528 6924 7149 7167 7191 7254
Size: 336 185 70 396 225 18 24 63
Cuts at: 7254 8373 9435 9520 10591 2400
Size: 1119 1062 85 1071 3183
Fragments arranged by size:
3183 1119 1071 1062 1015 831 518 396
336 294 282 273 238 225 185 86
85 70 63 24 18
BsiWI C'GTAC_G
Cuts at: 2984 2984
Size: 11374
BsmI GAATG_Cn'
Cuts at: 6822 7721 8885 8978 6822
Size: 899 1164 93 9218
Fragments arranged by size:
9218 1164 899 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3881 7426 7553 7750 8643 3881
Size: 3545 127 197 893 6612
Fragments arranged by size:
6612 3545 893 197 127
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2400 2583 3161 3231 3410 3441
Size: 399 306 304 183 578 70 179 31
Cuts at: 3441 3456 3581 3734 3740 3749 3787 3987 4092
Size: 15 125 153 6 9 38 200 105
Cuts at: 4092 4269 4401 4563 4649 4743 4773 4850 4922
Size: 177 132 162 86 94 30 77 72
Cuts at: 4922 5136 5220 5937 6273 6458 6528 6826 6924
Size: 214 84 717 336 185 70 298 98
Cuts at: 6924 7149 7167 7191 7254 7283 7484 7692 7943
Size: 225 18 24 63 29 201 208 251
Cuts at: 7943 7982 8140 8279 8373 8537 8581 8603 8693
Size: 39 158 139 94 164 44 22 90
Cuts at: 8693 9435 9520 10591 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 578 399 336 306
304 298 251 225 214 208 201 200
185 183 179 177 164 162 158 153
139 132 125 105 98 94 94 90
86 85 84 77 72 70 70 63
44 39 38 31 30 29 24 22
18 15 9 6
BspEI T'CCGG_A
Cuts at: 11037 11037
Size: 11374
BspHI T'CATG_A
Cuts at: 3168 3942 4269 4539 6762 7053 7191 9263 10993
Size: 774 327 270 2223 291 138 2072 1730
Cuts at: 10993 3168
Size: 3549
Fragments arranged by size:
3549 2223 2072 1730 774 327 291 270
138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2341 2613 4110 4578 4807 8729 844
Size: 1497 272 1497 468 229 3922 3489
Fragments arranged by size:
3922 3489 1497 1497 468 272 229
BsrBI CCG'CTC
Cuts at: 1394 1823 2860 5575 6042 8192 8626 9261 1394
Size: 429 1037 2715 467 2150 434 635 3507
Fragments arranged by size:
3507 2715 2150 1037 635 467 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3517 4036 5293 6777 9869 10043 65
Size: 1183 2269 519 1257 1484 3092 174 1396
Fragments arranged by size:
3092 2269 1484 1396 1257 1183 519 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3231 3390 3513 3687 5591 6315 6972
Size: 110 1421 159 123 174 1904 724 657
Cuts at: 6972 7301 7374 7511 8174 10018 10205 1700
Size: 329 73 137 663 1844 187 2869
Fragments arranged by size:
2869 1904 1844 1421 724 663 657 329
187 174 159 137 123 110 73
BsrGI T'GTAC_A
Cuts at: 96 6162 96
Size: 6066 5308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11374
BssSI C'ACGA_G
Cuts at: 3989 4259 8539 9434 10446 3989
Size: 270 4280 895 1012 4917
Fragments arranged by size:
4917 4280 1012 895 270
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2433 6850 7162 7546 7913 794
Size: 1639 4417 312 384 367 4255
Fragments arranged by size:
4417 4255 1639 384 367 312
BstBI TT'CG_AA
Cuts at: 10178 10178
Size: 11374
BstEII G'GTnAC_C
Cuts at: 1184 6508 7998 1184
Size: 5324 1490 4560
Fragments arranged by size:
5324 4560 1490
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 3277 4018 4588 4837 5569 5650 5806 6520
Size: 885 741 570 249 732 81 156 714
Cuts at: 6520 6547 7606 7699 8479 8596 2392
Size: 27 1059 93 780 117 5170
Fragments arranged by size:
5170 1059 885 780 741 732 714 570
249 156 117 93 81 27
BstYI r'GATC_y
Cuts at: 1972 2343 2592 2850 3315 4023 4452 4702 5802
Size: 371 249 258 465 708 429 250 1100
Cuts at: 5802 7753 7785 8704 9071 9456 9473 10925 11011
Size: 1951 32 919 367 385 17 1452 86
Cuts at: 11011 11023 11369 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1951 1452 1100 919 708 465 429
385 371 367 346 258 250 249 86
32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2552 2552
Size: 11374
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2240 2385 3466 4278 4934
Size: 552 939 66 170 145 1081 812 656
Cuts at: 4934 5067 5084 5220 5338 7029 7270 8076 8310
Size: 133 17 136 118 1691 241 806 234
Cuts at: 8310 8335 8518 8547 8589 513
Size: 25 183 29 42 3298
Fragments arranged by size:
3298 1691 1081 939 812 806 656 552
241 234 183 170 145 136 133 118
66 42 29 25 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5290 5548 487
Size: 683 56 4064 258 6313
Fragments arranged by size:
6313 4064 683 258 56
BtsI GCAGTG_nn'
Cuts at: 2618 4814 7914 8890 9684 9704 2618
Size: 2196 3100 976 794 20 4288
Fragments arranged by size:
4288 3100 2196 976 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6574 6609 529
Size: 35 6010 35 5294
Fragments arranged by size:
6010 5294 35 35
DraI TTT'AAA
Cuts at: 8678 8678
Size: 11374
DraIII CAC_nnn'GTG
Cuts at: 4766 5382 4766
Size: 616 10758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4475 5450 5528 10381 809
Size: 507 3159 975 78 4853 1802
Fragments arranged by size:
4853 3159 1802 975 507 78
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3279 3685 3948
Size: 54 1425 50 275 18 1449 406 263
Cuts at: 3948 4290 4578 5205 5640 6054 6672 6807 7321
Size: 342 288 627 435 414 618 135 514
Cuts at: 7321 7651 7740 8215 8481 8491 8572 8821 9710
Size: 330 89 475 266 10 81 249 889
Cuts at: 9710 11339 8
Size: 1629 43
Fragments arranged by size:
1629 1449 1425 889 627 618 514 475
435 414 406 342 330 288 275 266
263 249 135 89 81 54 50 43
18 10
EagI C'GGCC_G
Cuts at: 1537 1812 3685 7740 8215 8491 8572 8821 1537
Size: 275 1873 4055 475 276 81 249 4090
Fragments arranged by size:
4090 4055 1873 475 276 275 249 81
EarI CTCTTCn'nnn_
Cuts at: 1353 3562 4097 4322 4568 4656 5465 5522 5990
Size: 2209 535 225 246 88 809 57 468
Cuts at: 5990 6330 6884 6896 7022 8231 8414 8663 9304
Size: 340 554 12 126 1209 183 249 641
Cuts at: 9304 1353
Size: 3423
Fragments arranged by size:
3423 2209 1209 809 641 554 535 468
340 249 246 225 183 126 88 57
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3785 3863 4187 4619 5021 9961
Size: 1064 138 2196 78 324 432 402 4940
Cuts at: 9961 10335 10481 387
Size: 374 146 1280
Fragments arranged by size:
4940 2196 1280 1064 432 402 374 324
146 138 78
EcoICRI GAG'CTC
Cuts at: 3747 4647 4920 6922 7252 3747
Size: 900 273 2002 330 7869
Fragments arranged by size:
7869 2002 900 330 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2495 2747 3253 3263 4040 4079
Size: 393 506 357 252 506 10 777 39
Cuts at: 4079 4091 4315 4407 4661 4954 5269 5591 5900
Size: 12 224 92 254 293 315 322 309
Cuts at: 5900 6354 6395 6542 6569 6620 6632 6728 6812
Size: 454 41 147 27 51 12 96 84
Cuts at: 6812 7280 7849 8145 8212 8660 9437 10034 10821
Size: 468 569 296 67 448 777 597 787
Cuts at: 10821 11089 1239
Size: 268 1524
Fragments arranged by size:
1524 787 777 777 597 569 506 506
468 454 448 393 357 322 315 309
296 293 268 254 252 224 147 96
92 84 67 51 41 39 27 12
12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2891 3344 3374 4460 4589 6104 7323 8027 8160
Size: 453 30 1086 129 1515 1219 704 133
Cuts at: 8160 2891
Size: 6105
Fragments arranged by size:
6105 1515 1219 1086 704 453 133 129
30
EcoO109I rG'GnC_Cy
Cuts at: 2556 2929 3379 3400 3415 3438 3783 4778 4796
Size: 373 450 21 15 23 345 995 18
Cuts at: 4796 5133 5485 5812 6823 7280 7590 7630 8165
Size: 337 352 327 1011 457 310 40 535
Cuts at: 8165 8221 8563 8600 2556
Size: 56 342 37 5330
Fragments arranged by size:
5330 1011 995 535 457 450 373 352
345 342 337 327 310 56 40 37
23 21 18 15
EcoRI G'AATT_C
Cuts at: 8683 9061 8683
Size: 378 10996
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7459 7459
Size: 11374
FseI GG_CCGG'CC
Cuts at: 8178 8178
Size: 11374
FspI TGC'GCA
Cuts at: 3344 5837 6776 9880 3344
Size: 2493 939 3104 4838
Fragments arranged by size:
4838 3104 2493 939
HaeII r_GCGC'y
Cuts at: 1678 2019 2171 2926 2997 3259 3358 3397 4255
Size: 341 152 755 71 262 99 39 858
Cuts at: 4255 4900 5049 5232 5873 6373 6388 6640 7317
Size: 645 149 183 641 500 15 252 677
Cuts at: 7317 7381 8612 10521 1678
Size: 64 1231 1909 2531
Fragments arranged by size:
2531 1909 1231 858 755 677 645 641
500 341 262 252 183 152 149 99
71 64 39 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 3094 3098 3126
Size: 32 135 32 740 32 750 4 28
Cuts at: 3126 3130 3466 3498 3791 3823 4631 4663 4883
Size: 4 336 32 293 32 808 32 220
Cuts at: 4883 4915 5000 5032 5787 5819 7406 7438 7460
Size: 32 85 32 755 32 1587 32 22
Cuts at: 7460 7492 7577 7609 10015 10047 10089 10121 1373
Size: 32 85 32 2406 32 42 32 2626
Fragments arranged by size:
2626 2406 1587 808 755 750 740 336
293 220 135 85 85 42 32 32
32 32 32 32 32 32 32 32
32 32 32 32 28 22 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2956 3961 8011 8718 8964 669
Size: 988 281 1018 1005 4050 707 246 3079
Fragments arranged by size:
4050 3079 1018 1005 988 707 281 246
HindIII A'AGCT_T
Cuts at: 748 6846 748
Size: 6098 5276
HpaI GTT'AAC
Cuts at: 8964 8964
Size: 11374
KasI G'GCGC_C
Cuts at: 2167 2922 3393 4251 5045 5228 5869 6369 7313
Size: 755 471 858 794 183 641 500 944
Cuts at: 7313 8608 2167
Size: 1295 4933
Fragments arranged by size:
4933 1295 944 858 794 755 641 500
471 183
KpnI G_GTAC'C
Cuts at: 8699 8699
Size: 11374
MfeI C'AATT_G
Cuts at: 8973 8973
Size: 11374
MluI A'CGCG_T
Cuts at: 11250 11250
Size: 11374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 4677 5649 10488 10672 1305
Size: 1050 2322 972 4839 184 2007
Fragments arranged by size:
4839 2322 2007 1050 972 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3281 3950 4292 4580 6674
Size: 54 1425 343 1449 669 342 288 2094
Cuts at: 6674 6809 10
Size: 135 4575
Fragments arranged by size:
4575 2094 1449 1425 669 343 342 288
135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2264 2390 2477 2684 3009 3465
Size: 648 1059 39 126 87 207 325 456
Cuts at: 3465 3617 3944 4046 4586 4835 5327 5648 5771
Size: 152 327 102 540 249 492 321 123
Cuts at: 5771 6281 6815 6839 7172 7697 9332 9691 9850
Size: 510 534 24 333 525 1635 359 159
Cuts at: 9850 518
Size: 2042
Fragments arranged by size:
2042 1635 1059 648 540 534 525 510
492 456 359 333 327 325 321 249
207 159 152 126 123 102 87 39
24
MspA1I CmG'CkG
Cuts at: 1606 2054 2337 2718 2943 3250 3660 3752 4698
Size: 448 283 381 225 307 410 92 946
Cuts at: 4698 4974 5069 5086 5222 5340 5721 6069 6400
Size: 276 95 17 136 118 381 348 331
Cuts at: 6400 6664 7095 7126 7552 8078 8271 8337 8549
Size: 264 431 31 426 526 193 66 212
Cuts at: 8549 8584 8790 9467 10615 10860 1606
Size: 35 206 677 1148 245 2120
Fragments arranged by size:
2120 1148 946 677 526 448 431 426
410 381 381 348 331 307 283 276
264 245 225 212 206 193 136 118
95 92 66 35 31 17
NaeI GCC'GGC
Cuts at: 3392 3515 3689 5593 6317 6974 7376 7513 8176
Size: 123 174 1904 724 657 402 137 663
Cuts at: 8176 3392
Size: 6590
Fragments arranged by size:
6590 1904 724 663 657 402 174 137
123
NarI GG'CG_CC
Cuts at: 2168 2923 3394 4252 5046 5229 5870 6370 7314
Size: 755 471 858 794 183 641 500 944
Cuts at: 7314 8609 2168
Size: 1295 4933
Fragments arranged by size:
4933 1295 944 858 794 755 641 500
471 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 2385 3466 4278 4934 7270 8310
Size: 552 939 381 1081 812 656 2336 1040
Cuts at: 8310 8589 513
Size: 279 3298
Fragments arranged by size:
3298 2336 1081 1040 939 812 656 552
381 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11374
NgoMIV G'CCGG_C
Cuts at: 3390 3513 3687 5591 6315 6972 7374 7511 8174
Size: 123 174 1904 724 657 402 137 663
Cuts at: 8174 3390
Size: 6590
Fragments arranged by size:
6590 1904 724 663 657 402 174 137
123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11374
NotI GC'GGCC_GC
Cuts at: 8821 8821
Size: 11374
NsiI A_TGCA'T
Cuts at: 4350 4350
Size: 11374
NspI r_CATG'y
Cuts at: 1132 1229 2263 2299 4051 4306 6280 6980 8732
Size: 97 1034 36 1752 255 1974 700 1752
Cuts at: 8732 10277 1132
Size: 1545 2229
Fragments arranged by size:
2229 1974 1752 1752 1545 1034 700 255
97 36
PasI CC'CwG_GG
Cuts at: 1348 2413 3364 3403 4512 4843 8140 1348
Size: 1065 951 39 1109 331 3297 4582
Fragments arranged by size:
4582 3297 1109 1065 951 331 39
PciI A'CATG_T
Cuts at: 6276 10273 6276
Size: 3997 7377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3403 3616 4261 4766 6010 6469 7276 8524
Size: 2295 213 645 505 1244 459 807 1248
Cuts at: 8524 1108
Size: 3958
Fragments arranged by size:
3958 2295 1248 1244 807 645 505 459
213
PfoI T'CCnGG_A
Cuts at: 2610 2724 3740 4017 4677 4913 8161 2610
Size: 114 1016 277 660 236 3248 5823
Fragments arranged by size:
5823 3248 1016 660 277 236 114
PmeI GTTT'AAAC
Cuts at: 8678 8678
Size: 11374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5462 5494 9443 9475 10956 10988 4403
Size: 32 1027 32 3949 32 1481 32 4789
Fragments arranged by size:
4789 3949 1481 1027 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2929 4778 4796 5485 5812 7590 7630 8165 8563
Size: 1849 18 689 327 1778 40 535 398
Cuts at: 8563 2929
Size: 5740
Fragments arranged by size:
5740 1849 1778 689 535 398 327 40
18
PshAI GACnn'nnGTC
Cuts at: 1613 4752 7620 7827 1613
Size: 3139 2868 207 5160
Fragments arranged by size:
5160 3139 2868 207
PsiI TTA'TAA
Cuts at: 8944 8944
Size: 11374
PspOMI G'GGCC_C
Cuts at: 3406 3437 3783 5132 6822 7279 8599 3406
Size: 31 346 1349 1690 457 1320 6181
Fragments arranged by size:
6181 1690 1349 1320 457 346 31
PspXI vC'TCGA_Gb
Cuts at: 7253 8099 7253
Size: 846 10528
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2954 2986 6146 6178 2954
Size: 32 3160 32 8150
Fragments arranged by size:
8150 3160 32 32
PstI C_TGCA'G
Cuts at: 830 2041 2352 3387 4821 7113 7909 7977 8091
Size: 1211 311 1035 1434 2292 796 68 114
Cuts at: 8091 8726 830
Size: 635 3478
Fragments arranged by size:
3478 2292 1434 1211 1035 796 635 311
114 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11374
PvuII CAG'CTG
Cuts at: 3660 4974 5721 6664 7095 7552 3660
Size: 1314 747 943 431 457 7482
Fragments arranged by size:
7482 1314 943 747 457 431
RsrII CG'GwC_CG
Cuts at: 8551 8551
Size: 11374
SacI G_AGCT'C
Cuts at: 3749 4649 4922 6924 7254 3749
Size: 900 273 2002 330 7869
Fragments arranged by size:
7869 2002 900 330 273
SacII CC_GC'GG
Cuts at: 5070 5087 5223 5341 8079 8338 8550 5070
Size: 17 136 118 2738 259 212 7894
Fragments arranged by size:
7894 2738 259 212 136 118 17
SalI G'TCGA_C
Cuts at: 1936 8716 1936
Size: 6780 4594
SapI GCTCTTCn'nnn_
Cuts at: 4097 4322 4568 5990 6896 4097
Size: 225 246 1422 906 8575
Fragments arranged by size:
8575 1422 906 246 225
SbfI CC_TGCA'GG
Cuts at: 2352 8726 2352
Size: 6374 5000
ScaI AGT'ACT
Cuts at: 2498 3926 6566 9622 11334 2498
Size: 1428 2640 3056 1712 2538
Fragments arranged by size:
3056 2640 2538 1712 1428
SfcI C'TryA_G
Cuts at: 826 1045 2037 2348 3383 4817 7109 7905 7973
Size: 219 992 311 1035 1434 2292 796 68
Cuts at: 7973 8087 8722 9857 10538 10729 826
Size: 114 635 1135 681 191 1471
Fragments arranged by size:
2292 1471 1434 1135 1035 992 796 681
635 311 219 191 114 68
SfoI GGC'GCC
Cuts at: 2169 2924 3395 4253 5047 5230 5871 6371 7315
Size: 755 471 858 794 183 641 500 944
Cuts at: 7315 8610 2169
Size: 1295 4933
Fragments arranged by size:
4933 1295 944 858 794 755 641 500
471 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11374
SmaI CCC'GGG
Cuts at: 1872 5051 5240 5272 7311 8171 8289 8701 1872
Size: 3179 189 32 2039 860 118 412 4545
Fragments arranged by size:
4545 3179 2039 860 412 189 118 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2399 2861 4985 5912 6560 7253
Size: 197 926 456 462 2124 927 648 693
Cuts at: 7253 7625 8099 9478 10379 10641 820
Size: 372 474 1379 901 262 1553
Fragments arranged by size:
2124 1553 1379 927 926 901 693 648
474 462 456 372 262 197
SnaBI TAC'GTA
Cuts at: 493 9165 493
Size: 8672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11374
SphI G_CATG'C
Cuts at: 1132 2263 4306 6980 8732 1132
Size: 1131 2043 2674 1752 3774
Fragments arranged by size:
3774 2674 2043 1752 1131
SrfI GCCC'GGGC
Cuts at: 8289 8289
Size: 11374
SspI AAT'ATT
Cuts at: 5 52 9298 5
Size: 47 9246 2081
Fragments arranged by size:
9246 2081 47
StuI AGG'CCT
Cuts at: 1517 2115 4686 7568 1517
Size: 598 2571 2882 5323
Fragments arranged by size:
5323 2882 2571 598
StyI C'CwwG_G
Cuts at: 513 1065 2004 2385 2892 3447 3466 4278 4339
Size: 552 939 381 507 555 19 812 61
Cuts at: 4339 4774 4934 6138 7270 7443 8003 8310 8589
Size: 435 160 1204 1132 173 560 307 279
Cuts at: 8589 8604 8813 513
Size: 15 209 3074
Fragments arranged by size:
3074 1204 1132 939 812 560 555 552
507 435 381 307 279 209 173 160
61 19 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2172 3070 4475 4754 6139 6275 6666
Size: 1255 592 898 1405 279 1385 136 391
Cuts at: 6666 7094 7598 8312 9401 9418 9571 9756 10201
Size: 428 504 714 1089 17 153 185 445
Cuts at: 10201 325
Size: 1498
Fragments arranged by size:
1498 1405 1385 1255 1089 898 714 592
504 445 428 391 279 185 153 136
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 3093 3924
Size: 274 80 33 51 1443 519 597 831
Cuts at: 3924 5688 6162 6231 6564 7128 9620 11332 96
Size: 1764 474 69 333 564 2492 1712 138
Fragments arranged by size:
2492 1764 1712 1443 831 597 564 519
474 333 274 138 80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4380 4412 7391 7423 7484 7516 8291 8323 11334
Size: 32 2979 32 61 32 775 32 3011
Cuts at: 11334 11366 4380
Size: 32 4388
Fragments arranged by size:
4388 3011 2979 775 61 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2900 5173 5525 7418 1316
Size: 1584 2273 352 1893 5272
Fragments arranged by size:
5272 2273 1893 1584 352
XbaI T'CTAG_A
Cuts at: 8710 8710
Size: 11374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3530 4285 4741 4759 6466 3530
Size: 755 456 18 1707 8438
Fragments arranged by size:
8438 1707 755 456 18
XhoI C'TCGA_G
Cuts at: 1943 7253 7625 8099 1943
Size: 5310 372 474 5218
Fragments arranged by size:
5310 5218 474 372
XmaI C'CCGG_G
Cuts at: 1870 5049 5238 5270 7309 8169 8287 8699 1870
Size: 3179 189 32 2039 860 118 412 4545
Fragments arranged by size:
4545 3179 2039 860 412 189 118 32
XmnI GAAnn'nnTTC
Cuts at: 4412 5477 9503 4412
Size: 1065 4026 6283
Fragments arranged by size:
6283 4026 1065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2902 5317 5410 9182
Size: 53 83 186 936 1368 2415 93 3772
Cuts at: 9182 276
Size: 2468
Fragments arranged by size:
3772 2468 2415 1368 936 186 93 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BstZ17I EcoRV FspAI NruI PacI
PmlI SanDI SexAI SfiI SwaI