Restriction map for
pFN21AE2484
(Circular) (Six-base) MAPSORT of: pFN21AE2484.seq Check: 1810 from: 1 to: 11836
pFN21AE2484 11836 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3368 5530 7472 3368
Size: 2162 1942 7732
Fragments arranged by size:
7732 2162 1942
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7967 9646 278
Size: 53 83 186 936 6431 1679 2468
Fragments arranged by size:
6431 2468 1679 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3075 4971 9179 1937
Size: 1138 1896 4208 4594
Fragments arranged by size:
4594 4208 1896 1138
Acc65I G'GTAC_C
Cuts at: 9157 9157
Size: 11836
AclI AA'CG_TT
Cuts at: 1563 9963 10336 1563
Size: 8400 373 3063
Fragments arranged by size:
8400 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 4400 4550 4624 5381 5601 6218 6953
Size: 2198 570 150 74 757 220 617 735
Cuts at: 6953 7184 7193 7865 8753 9899 11283 11551 1632
Size: 231 9 672 888 1146 1384 268 1917
Fragments arranged by size:
2198 1917 1384 1146 888 757 735 672
617 570 268 231 220 150 74 9
AfeI AGC'GCT
Cuts at: 2342 2774 5299 5917 6754 2342
Size: 432 2525 618 837 7424
Fragments arranged by size:
7424 2525 837 618 432
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11639
AflIII A'CryG_T
Cuts at: 1203 6594 8160 10735 11712 1203
Size: 5391 1566 2575 977 1327
Fragments arranged by size:
5391 2575 1566 1327 977
AgeI A'CCGG_T
Cuts at: 6384 10667 6384
Size: 4283 7553
AhdI GACnn_n'nnGTC
Cuts at: 5764 7456 10565 5764
Size: 1692 3109 7035
Fragments arranged by size:
7035 3109 1692
AleI CACnn'nnGTG
Cuts at: 1166 7329 1166
Size: 6163 5673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2663 2873 4880 5577 1184
Size: 1479 210 2007 697 7443
Fragments arranged by size:
7443 2007 1479 697 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 11804
AlwNI CAG_nnn'CTG
Cuts at: 3447 3659 4762 5234 5292 6196 6430 6919 7222
Size: 212 1103 472 58 904 234 489 303
Cuts at: 7222 8208 8440 9579 11151 3447
Size: 986 232 1139 1572 4132
Fragments arranged by size:
4132 1572 1139 1103 986 904 489 472
303 234 232 212 58
ApaI G_GGCC'C
Cuts at: 3547 3640 3852 3888 4157 5195 5638 7697 8193
Size: 93 212 36 269 1038 443 2059 496
Cuts at: 8193 8481 8627 3547
Size: 288 146 6756
Fragments arranged by size:
6756 2059 1038 496 443 288 269 212
146 93 36
ApaLI G'TGCA_C
Cuts at: 3488 3662 3782 5349 6826 9893 11049 3488
Size: 174 120 1567 1477 3067 1156 4275
Fragments arranged by size:
4275 3067 1567 1477 1156 174 120
ApoI r'AATT_y
Cuts at: 1493 2740 4717 9145 9371 9523 11632 1493
Size: 1247 1977 4428 226 152 2109 1697
Fragments arranged by size:
4428 2109 1977 1697 1247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10390 160
Size: 872 9358 1606
Fragments arranged by size:
9358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11836
AvaI C'yCGr_G
Cuts at: 1870 1943 2125 3369 3416 3549 3881 4017 4307
Size: 73 182 1244 47 133 332 136 290
Cuts at: 4307 5631 5750 7155 8496 8951 9161 1870
Size: 1324 119 1405 1341 455 210 4545
Fragments arranged by size:
4545 1405 1341 1324 1244 455 332 290
210 182 136 133 119 73 47
BamHI G'GATC_C
Cuts at: 2349 5721 9028 9166 9533 2349
Size: 3372 3307 138 367 4652
Fragments arranged by size:
4652 3372 3307 367 138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2584 3002 3712 3929
Size: 325 217 627 243 554 418 710 217
Cuts at: 3929 5542 5622 6113 8339 8397 9157 10612 618
Size: 1613 80 491 2226 58 760 1455 1842
Fragments arranged by size:
2226 1842 1613 1455 760 710 627 554
491 418 325 243 217 217 80 58
BanII G_rGCy'C
Cuts at: 1391 2807 3152 3291 3547 3640 3852 3888 4044
Size: 1416 345 139 256 93 212 36 156
Cuts at: 4044 4157 4170 5013 5195 5441 5638 5731 7296
Size: 113 13 843 182 246 197 93 1565
Cuts at: 7296 7697 7729 7829 8193 8217 8481 8627 8715
Size: 401 32 100 364 24 264 146 88
Cuts at: 8715 9010 9155 1391
Size: 295 145 4072
Fragments arranged by size:
4072 1565 1416 843 401 364 345 295
264 256 246 212 197 182 156 146
145 139 113 100 93 93 88 36
32 24 13
BbeI G_GCGC'C
Cuts at: 2034 3006 3716 5626 8401 2034
Size: 972 710 1910 2775 5469
Fragments arranged by size:
5469 2775 1910 972 710
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2659 4339 4589 6694 7637 8460 928
Size: 971 760 1680 250 2105 943 823 4304
Fragments arranged by size:
4304 2105 1680 971 943 823 760 250
BbvCI CC'TCA_GC
Cuts at: 6134 7637 6134
Size: 1503 10333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 5863 5897 6713 6747 10027
Size: 34 2333 34 1559 34 816 34 3280
Cuts at: 10027 10061 1903
Size: 34 3678
Fragments arranged by size:
3678 3280 2333 1559 816 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2614 3783 6331 9728 10938 2614
Size: 1169 2548 3397 1210 3512
Fragments arranged by size:
3512 3397 2548 1210 1169
BclI T'GATC_A
Cuts at: 1548 3162 6177 1548
Size: 1614 3015 7207
Fragments arranged by size:
7207 3015 1614
BfrBI ATG'CAT
Cuts at: 4657 4657
Size: 11836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3551 5466 7875
Size: 107 122 71 631 605 1879 1915 2409
Cuts at: 7875 7914 8395 10447 136
Size: 39 481 2052 1525
Fragments arranged by size:
2409 2052 1915 1879 1525 631 605 481
122 107 71 39
BglII A'GATC_T
Cuts at: 3951 6936 8562 11831 3951
Size: 2985 1626 3269 3956
Fragments arranged by size:
3956 3269 2985 1626
BlpI GC'TnA_GC
Cuts at: 2974 3398 6745 7611 8666 9253 2974
Size: 424 3347 866 1055 587 5557
Fragments arranged by size:
5557 3347 1055 866 587 424
Bme1580I G_kGCm'C
Cuts at: 1790 2589 2862 3492 3547 3640 3666 3786 3852
Size: 799 273 630 55 93 26 120 66
Cuts at: 3852 3888 4018 4157 5195 5353 5638 6830 7697
Size: 36 130 139 1038 158 285 1192 867
Cuts at: 7697 8053 8193 8342 8481 8627 8858 9897 11053
Size: 356 140 149 139 146 231 1039 1156
Cuts at: 11053 1790
Size: 2573
Fragments arranged by size:
2573 1192 1156 1039 1038 867 799 630
356 285 273 231 158 149 146 140
139 139 130 120 93 66 55 36
26
BmgBI CAC'GTC
Cuts at: 1318 4812 1318
Size: 3494 8342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6008 6763 7513
Size: 416 53 83 399 24 4591 755 750
Cuts at: 7513 7670 8623 9567 10525 442
Size: 157 953 944 958 1753
Fragments arranged by size:
4591 1753 958 953 944 755 750 416
399 157 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3279 1056
Size: 2223 9613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4189 5455 5750 5776 9024 4189
Size: 1266 295 26 3248 7001
Fragments arranged by size:
7001 3248 1266 295 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 2789 2911 3083 3932 3940 4210 4388
Size: 1280 270 122 172 849 8 270 178
Cuts at: 4388 5311 5314 5516 6713 7100 7328 7586 7637
Size: 923 3 202 1197 387 228 258 51
Cuts at: 7637 7643 7793 7853 8075 8297 8613 10496 1239
Size: 6 150 60 222 222 316 1883 2579
Fragments arranged by size:
2579 1883 1280 1197 923 849 387 316
270 270 258 228 222 222 202 178
172 150 122 60 51 8 6 3
Bpu10I CC'TnA_GC
Cuts at: 2567 2756 3360 3539 5693 6134 6170 7637 2567
Size: 189 604 179 2154 441 36 1467 6766
Fragments arranged by size:
6766 2154 1467 604 441 189 179 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2215 3299 9961 10826 11124 2215
Size: 1084 6662 865 298 2927
Fragments arranged by size:
6662 2927 1084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3197 3370 3422 6106 7296 8627 8676
Size: 617 1698 173 52 2684 1190 1331 49
Cuts at: 8676 10499 882
Size: 1823 2219
Fragments arranged by size:
2684 2219 1823 1698 1331 1190 617 173
52 49
BsaAI yAC'GTr
Cuts at: 493 1204 4864 5845 9627 493
Size: 711 3660 981 3782 2702
Fragments arranged by size:
3782 3660 2702 981 711
BsaBI GATnn'nnATC
Cuts at: 3041 4259 11830 3041
Size: 1218 7571 3047
Fragments arranged by size:
7571 3047 1218
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2691 3003 3620
Size: 53 83 186 936 498 660 312 617
Cuts at: 3620 3713 5623 5673 7964 8398 9643 10025 10660
Size: 93 1910 50 2291 434 1245 382 635
Cuts at: 10660 275
Size: 1451
Fragments arranged by size:
2291 1910 1451 1245 936 660 635 617
498 434 382 312 186 93 83 53
50
BsaWI w'CCGG_w
Cuts at: 3799 4710 5814 6384 8997 9101 10269 10667 10941
Size: 911 1104 570 2613 104 1168 398 274
Cuts at: 10941 11088 11499 3799
Size: 147 411 4136
Fragments arranged by size:
4136 2613 1168 1104 911 570 411 398
274 147 104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2209 2239 2909 2939 3778
Size: 30 165 30 806 30 670 30 839
Cuts at: 3778 3808 6008 6038 7940 7970 8822 8852 1178
Size: 30 2200 30 1902 30 852 30 4162
Fragments arranged by size:
4162 2200 1902 852 839 806 670 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2030 2051 3125 3320 4325 5447 5462
Size: 841 3 21 1074 195 1005 1122 15
Cuts at: 5462 5775 6668 6686 6800 7583 7607 8723 8815
Size: 313 893 18 114 783 24 1116 92
Cuts at: 8815 9016 1186
Size: 201 4006
Fragments arranged by size:
4006 1122 1116 1074 1005 893 841 783
313 201 195 114 92 24 21 18
15 3
BseYI C'CCAG_C
Cuts at: 2337 2588 2635 2820 3069 3406 3432 3437 4104
Size: 251 47 185 249 337 26 5 667
Cuts at: 4104 4169 5027 5188 5264 5354 7644 7735 7904
Size: 65 858 161 76 90 2290 91 169
Cuts at: 7904 8208 8256 8978 9118 11039 11535 2337
Size: 304 48 722 140 1921 496 2638
Fragments arranged by size:
2638 2290 1921 858 722 667 496 337
304 251 249 185 169 161 140 91
90 76 65 48 47 26 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6317 7715 1163
Size: 573 4581 1398 5284
Fragments arranged by size:
5284 4581 1398 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2099 2564 4051 5136 5347 9286
Size: 275 186 98 465 1487 1085 211 3939
Cuts at: 9286 10047 11075 1540
Size: 761 1028 2301
Fragments arranged by size:
3939 2301 1487 1085 1028 761 465 275
211 186 98
BsiHKAI G_wGCw'C
Cuts at: 2988 3492 3666 3786 4044 4578 4869 5088 5165
Size: 504 174 120 258 534 291 219 77
Cuts at: 5165 5353 5441 5592 6069 6830 6900 7296 7779
Size: 188 88 151 477 761 70 396 483
Cuts at: 7779 8180 9897 9982 11053 2988
Size: 401 1717 85 1071 3771
Fragments arranged by size:
3771 1717 1071 761 534 504 483 477
401 396 291 258 219 188 174 151
120 88 85 77 70
BsmI GAATG_Cn'
Cuts at: 9347 9440 9347
Size: 93 11743
BsmBI CGTCTCn'nnnn_
Cuts at: 3797 6206 3797
Size: 2409 9427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2589 2807 2862 2988 3152 3291 3492
Size: 399 799 218 55 126 164 139 201
Cuts at: 3492 3547 3640 3666 3786 3852 3888 4018 4044
Size: 55 93 26 120 66 36 130 26
Cuts at: 4044 4157 4170 4578 4869 5013 5088 5165 5195
Size: 113 13 408 291 144 75 77 30
Cuts at: 5195 5353 5441 5592 5638 5731 6069 6830 6900
Size: 158 88 151 46 93 338 761 70
Cuts at: 6900 7296 7697 7729 7779 7829 8053 8180 8193
Size: 396 401 32 50 50 224 127 13
Cuts at: 8193 8217 8342 8481 8627 8715 8858 9010 9155
Size: 24 125 139 146 88 143 152 145
Cuts at: 9155 9897 9982 11053 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 799 761 742 408 401 399
396 338 291 224 218 201 164 158
152 151 146 145 144 143 139 139
130 127 126 125 120 113 93 93
88 88 85 77 75 70 66 55
55 50 50 46 36 32 30 26
26 24 13 13
BspEI T'CCGG_A
Cuts at: 8997 11499 8997
Size: 2502 9334
BspHI T'CATG_A
Cuts at: 3159 4251 4578 4845 6522 7134 9725 11455 3159
Size: 1092 327 267 1677 612 2591 1730 3540
Fragments arranged by size:
3540 2591 1730 1677 1092 612 327 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2523 3368 5530 5532 6036 7472 8466 9191
Size: 1679 845 2162 2 504 1436 994 725
Cuts at: 9191 844
Size: 3489
Fragments arranged by size:
3489 2162 1679 1436 994 845 725 504
2
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 2566 4687 5386 5452 5500 6169
Size: 429 204 539 2121 699 66 48 669
Cuts at: 6169 9723 1394
Size: 3554 3507
Fragments arranged by size:
3554 3507 2121 699 669 539 429 204
66 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 3535 4836 5608 7149 10331 10505 65
Size: 1183 2287 1301 772 1541 3182 174 1396
Fragments arranged by size:
3182 2287 1541 1396 1301 1183 772 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 2463 3594 4134 4549 6384 8772
Size: 110 284 369 1131 540 415 1835 2388
Cuts at: 8772 8904 10480 10667 1700
Size: 132 1576 187 2869
Fragments arranged by size:
2869 2388 1835 1576 1131 540 415 369
284 187 132 110
BsrGI T'GTAC_A
Cuts at: 96 3327 96
Size: 3231 8605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11836
BssSI C'ACGA_G
Cuts at: 4298 5246 9896 10908 4298
Size: 948 4650 1012 5226
Fragments arranged by size:
5226 4650 1012 948
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3244 3447 3907 7222 8143 794
Size: 2450 203 460 3315 921 4487
Fragments arranged by size:
4487 3315 2450 921 460 203
BstBI TT'CG_AA
Cuts at: 10640 10640
Size: 11836
BstEII G'GTnAC_C
Cuts at: 1184 6880 1184
Size: 5696 6140
BstXI CCAn_nnnn'nTGG
Cuts at: 3271 4894 5887 6124 6892 8888 3271
Size: 1623 993 237 768 1996 6219
Fragments arranged by size:
6219 1996 1623 993 768 237
BstYI r'GATC_y
Cuts at: 1972 2349 3564 3951 4758 5721 6120 6936 7929
Size: 377 1215 387 807 963 399 816 993
Cuts at: 7929 8562 9028 9166 9533 9918 9935 11387 11473
Size: 633 466 138 367 385 17 1452 86
Cuts at: 11473 11485 11831 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1215 993 963 816 807 633
466 399 387 385 377 367 346 138
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2213 2391 4587 5886 6096 6885
Size: 552 939 209 178 2196 1299 210 789
Cuts at: 6885 6891 7782 8079 8723 513
Size: 6 891 297 644 3626
Fragments arranged by size:
3626 2196 1299 939 891 789 644 552
297 210 209 178 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2308 2470 5462 5540 5866 487
Size: 683 56 1082 162 2992 78 326 6457
Fragments arranged by size:
6457 2992 1082 683 326 162 78 56
BtsI GCAGTG_nn'
Cuts at: 5476 5534 5559 8178 9352 10146 10166 5476
Size: 58 25 2619 1174 794 20 7146
Fragments arranged by size:
7146 2619 1174 794 58 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11801
DraI TTT'AAA
Cuts at: 9140 9140
Size: 11836
DraIII CAC_nnn'GTG
Cuts at: 3487 3487
Size: 11836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5846 6220 10843 809
Size: 507 4530 374 4623 1802
Fragments arranged by size:
4623 4530 1802 507 374
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2309 4240
Size: 54 1425 50 275 18 266 213 1931
Cuts at: 4240 4599 5133 5344 5569 6421 6888 7044 8701
Size: 359 534 211 225 852 467 156 1657
Cuts at: 8701 9283 10172 11801 8
Size: 582 889 1629 43
Fragments arranged by size:
1931 1657 1629 1425 889 852 582 534
467 359 275 266 225 213 211 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2096 5133 5344 9283 1537
Size: 275 284 3037 211 3939 4090
Fragments arranged by size:
4090 3939 3037 284 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 4631 4667 4874 5096 5424 5439 6308 6645
Size: 3278 36 207 222 328 15 869 337
Cuts at: 6645 6854 7256 7394 9766 1353
Size: 209 402 138 2372 3423
Fragments arranged by size:
3423 3278 2372 869 402 337 328 222
209 207 138 36 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2162 4502 4925 5789 10423 10797
Size: 1064 138 573 2340 423 864 4634 374
Cuts at: 10797 10943 387
Size: 146 1280
Fragments arranged by size:
4634 2340 1280 1064 864 573 423 374
146 138
EcoICRI GAG'CTC
Cuts at: 4042 5439 7294 4042
Size: 1397 1855 8584
Fragments arranged by size:
8584 1855 1397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2789 2911 3083 3830 3932 3940
Size: 393 887 270 122 172 747 102 8
Cuts at: 3940 4210 4388 4400 4550 4624 5311 5314 5381
Size: 270 178 12 150 74 687 3 67
Cuts at: 5381 5516 5601 6218 6713 6953 7100 7184 7193
Size: 135 85 617 495 240 147 84 9
Cuts at: 7193 7328 7586 7637 7643 7793 7853 7865 8075
Size: 135 258 51 6 150 60 12 210
Cuts at: 8075 8297 8613 8753 9899 10496 11283 11551 1239
Size: 222 316 140 1146 597 787 268 1524
Fragments arranged by size:
1524 1146 887 787 747 687 617 597
495 393 316 270 270 268 258 240
222 210 178 172 150 150 147 140
135 135 122 102 85 84 74 67
60 51 12 12 9 8 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3338 4189 4766 5180 6422 7226 8595 3338
Size: 851 577 414 1242 804 1369 6579
Fragments arranged by size:
6579 1369 1242 851 804 577 414
EcoO109I rG'GnC_Cy
Cuts at: 2122 3543 3544 3588 3636 3637 3726 3849 3885
Size: 1421 1 44 48 1 89 123 36
Cuts at: 3885 4123 5192 5490 5635 6427 7219 7336 7693
Size: 238 1069 298 145 792 792 117 357
Cuts at: 7693 7694 8189 8190 8478 8623 8624 8728 8894
Size: 1 495 1 288 145 1 104 166
Cuts at: 8894 8963 2122
Size: 69 4995
Fragments arranged by size:
4995 1421 1069 792 792 495 357 298
288 238 166 145 145 123 117 104
89 69 48 44 36 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 9145 9523 9145
Size: 378 11458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5077 5533 6424 9049 5077
Size: 456 891 2625 7864
Fragments arranged by size:
7864 2625 891 456
FseI GG_CCGG'CC
Cuts at: 2098 2098
Size: 11836
FspI TGC'GCA
Cuts at: 2597 7148 10342 2597
Size: 4551 3194 4091
Fragments arranged by size:
4551 4091 3194
FspAI rTGC'GCAy
Cuts at: 2597 2597
Size: 11836
HaeII r_GCGC'y
Cuts at: 1678 2034 2171 2344 2776 3006 3716 4556 5189
Size: 356 137 173 432 230 710 840 633
Cuts at: 5189 5271 5301 5326 5626 5896 5919 6682 6756
Size: 82 30 25 300 270 23 763 74
Cuts at: 6756 7199 8328 8401 8773 10983 1678
Size: 443 1129 73 372 2210 2531
Fragments arranged by size:
2531 2210 1129 840 763 710 633 443
432 372 356 300 270 230 173 137
82 74 73 30 25 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 3085 3117 5006
Size: 32 135 32 637 32 844 32 1889
Cuts at: 5006 5038 5240 5272 5375 5407 5750 5782 6694
Size: 32 202 32 103 32 343 32 912
Cuts at: 6694 6726 7442 7474 7793 7825 7936 7940 7961
Size: 32 716 32 319 32 111 4 21
Cuts at: 7961 7968 7972 7993 8702 8734 8796 8822 8828
Size: 7 4 21 709 32 62 26 6
Cuts at: 8828 8854 8947 8979 10477 10509 10551 10583 1373
Size: 26 93 32 1498 32 42 32 2626
Fragments arranged by size:
2626 1889 1498 912 844 716 709 637
343 319 202 135 111 103 93 62
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
26 26 21 21 7 6 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2947 3076 4270 4972 7096 7534
Size: 988 281 1009 129 1194 702 2124 438
Cuts at: 7534 9180 9426 669
Size: 1646 246 3079
Fragments arranged by size:
3079 2124 1646 1194 1009 988 702 438
281 246 129
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11836
HpaI GTT'AAC
Cuts at: 9426 9426
Size: 11836
KasI G'GCGC_C
Cuts at: 2030 3002 3712 5622 8397 2030
Size: 972 710 1910 2775 5469
Fragments arranged by size:
5469 2775 1910 972 710
KpnI G_GTAC'C
Cuts at: 9161 9161
Size: 11836
MfeI C'AATT_G
Cuts at: 7411 9435 7411
Size: 2024 9812
MluI A'CGCG_T
Cuts at: 11712 11712
Size: 11836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3282 6020 6813 7944 8634 10950 11134 1305
Size: 1977 2738 793 1131 690 2316 184 2007
Fragments arranged by size:
2738 2316 2007 1977 1131 793 690 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4601 6423 6890 7046 10
Size: 54 1425 343 2769 1822 467 156 4800
Fragments arranged by size:
4800 2769 1822 1425 467 343 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 3143 4457 4892 5144 5885 6089
Size: 648 1098 879 1314 435 252 741 204
Cuts at: 6089 6599 6890 7139 7211 7329 9794 10153 10312
Size: 510 291 249 72 118 2465 359 159
Cuts at: 10312 518
Size: 2042
Fragments arranged by size:
2465 2042 1314 1098 879 741 648 510
435 359 291 252 249 204 159 118
72
MspA1I CmG'CkG
Cuts at: 1606 2215 2537 2698 2978 3214 3226 3297 3447
Size: 609 322 161 280 236 12 71 150
Cuts at: 3447 3705 3762 4002 4561 4567 4963 5220 5310
Size: 258 57 240 559 6 396 257 90
Cuts at: 5310 6111 7036 7739 7795 8823 8835 9252 9929
Size: 801 925 703 56 1028 12 417 677
Cuts at: 9929 11077 11322 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1028 925 801 703 677 609
559 417 396 322 280 258 257 245
240 236 161 150 90 71 57 56
12 12 6
NaeI GCC'GGC
Cuts at: 2096 3596 4136 4551 8774 8906 2096
Size: 1500 540 415 4223 132 5026
Fragments arranged by size:
5026 4223 1500 540 415 132
NarI GG'CG_CC
Cuts at: 2031 3003 3713 5623 8398 2031
Size: 972 710 1910 2775 5469
Fragments arranged by size:
5469 2775 1910 972 710
NcoI C'CATG_G
Cuts at: 513 1065 2004 4587 5886 6096 6885 6891 513
Size: 552 939 2583 1299 210 789 6 5458
Fragments arranged by size:
5458 2583 1299 939 789 552 210 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11836
NgoMIV G'CCGG_C
Cuts at: 2094 3594 4134 4549 8772 8904 2094
Size: 1500 540 415 4223 132 5026
Fragments arranged by size:
5026 4223 1500 540 415 132
NheI G'CTAG_C
Cuts at: 1052 3275 1052
Size: 2223 9613
NotI GC'GGCC_GC
Cuts at: 9283 9283
Size: 11836
NsiI A_TGCA'T
Cuts at: 4659 4659
Size: 11836
NspI r_CATG'y
Cuts at: 1132 1229 2269 2305 2596 2842 4615 4657 6133
Size: 97 1040 36 291 246 1773 42 1476
Cuts at: 6133 6598 7192 8032 8164 9194 10739 1132
Size: 465 594 840 132 1030 1545 2229
Fragments arranged by size:
2229 1773 1545 1476 1040 1030 840 594
465 291 246 132 97 42 36
PasI CC'CwG_GG
Cuts at: 1348 2296 4978 5077 7871 8194 1348
Size: 948 2682 99 2794 323 4990
Fragments arranged by size:
4990 2794 2682 948 323 99
PciI A'CATG_T
Cuts at: 6594 8160 10735 6594
Size: 1566 2575 7695
Fragments arranged by size:
7695 2575 1566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3484 5663 6841 6919 1108
Size: 2376 2179 1178 78 6025
Fragments arranged by size:
6025 2376 2179 1178 78
PfoI T'CCnGG_A
Cuts at: 3567 4119 7017 7440 3567
Size: 552 2898 423 7963
Fragments arranged by size:
7963 2898 552 423
PmeI GTTT'AAAC
Cuts at: 9140 9140
Size: 11836
PmlI CAC'GTG
Cuts at: 5845 5845
Size: 11836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8125 8157 9905 9937 11418 11450 8125
Size: 32 1748 32 1481 32 8511
Fragments arranged by size:
8511 1748 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 3588 3726 4123 5490 8728 8963 2122
Size: 1466 138 397 1367 3238 235 4995
Fragments arranged by size:
4995 3238 1466 1367 397 235 138
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11836
PsiI TTA'TAA
Cuts at: 9406 9406
Size: 11836
PspOMI G'GGCC_C
Cuts at: 3543 3636 3848 3884 4153 5191 5634 7693 8189
Size: 93 212 36 269 1038 443 2059 496
Cuts at: 8189 8477 8623 3543
Size: 288 146 6756
Fragments arranged by size:
6756 2059 1038 496 443 288 269 212
146 93 36
PspXI vC'TCGA_Gb
Cuts at: 5750 5750
Size: 11836
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 11804
PstI C_TGCA'G
Cuts at: 830 2358 2520 2852 4560 5541 8286 9188 830
Size: 1528 162 332 1708 981 2745 902 3478
Fragments arranged by size:
3478 2745 1708 1528 981 902 332 162
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11836
PvuII CAG'CTG
Cuts at: 2537 2698 3226 3297 3447 4002 4567 4963 5310
Size: 161 528 71 150 555 565 396 347
Cuts at: 5310 7036 7795 8823 2537
Size: 1726 759 1028 5550
Fragments arranged by size:
5550 1726 1028 759 565 555 528 396
347 161 150 71
SacI G_AGCT'C
Cuts at: 4044 5441 7296 4044
Size: 1397 1855 8584
Fragments arranged by size:
8584 1855 1397
SacII CC_GC'GG
Cuts at: 2216 2216
Size: 11836
SalI G'TCGA_C
Cuts at: 1936 3074 4970 9178 1936
Size: 1138 1896 4208 4594
Fragments arranged by size:
4594 4208 1896 1138
SanDI GG'GwC_CC
Cuts at: 3588 4123 5490 3588
Size: 535 1367 9934
Fragments arranged by size:
9934 1367 535
SapI GCTCTTCn'nnn_
Cuts at: 4631 4874 5096 6308 4631
Size: 243 222 1212 10159
Fragments arranged by size:
10159 1212 243 222
SbfI CC_TGCA'GG
Cuts at: 2358 9188 2358
Size: 6830 5006
ScaI AGT'ACT
Cuts at: 2504 2960 4235 10084 11796 2504
Size: 456 1275 5849 1712 2544
Fragments arranged by size:
5849 2544 1712 1275 456
SexAI A'CCwGG_T
Cuts at: 3321 3483 3321
Size: 162 11674
SfcI C'TryA_G
Cuts at: 826 1045 2354 2516 2848 3889 4556 5537 8282
Size: 219 1309 162 332 1041 667 981 2745
Cuts at: 8282 8743 9184 10319 11000 11191 826
Size: 461 441 1135 681 191 1471
Fragments arranged by size:
2745 1471 1309 1135 1041 981 681 667
461 441 332 219 191 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 3551 7875 3551
Size: 4324 7512
SfoI GGC'GCC
Cuts at: 2032 3004 3714 5624 8399 2032
Size: 972 710 1910 2775 5469
Fragments arranged by size:
5469 2775 1910 972 710
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11836
SmaI CCC'GGG
Cuts at: 1872 2127 3551 3883 4019 5633 8498 9163 1872
Size: 255 1424 332 136 1614 2865 665 4545
Fragments arranged by size:
4545 2865 1614 1424 665 332 255 136
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2194 3314 5750 9940 10841 11103
Size: 197 926 251 1120 2436 4190 901 262
Cuts at: 11103 820
Size: 1553
Fragments arranged by size:
4190 2436 1553 1120 926 901 262 251
197
SnaBI TAC'GTA
Cuts at: 493 9627 493
Size: 9134 2702
SpeI A'CTAG_T
Cuts at: 152 3823 152
Size: 3671 8165
SphI G_CATG'C
Cuts at: 1132 2596 2842 4615 4657 6133 7192 9194 1132
Size: 1464 246 1773 42 1476 1059 2002 3774
Fragments arranged by size:
3774 2002 1773 1476 1464 1059 246 42
SrfI GCCC'GGGC
Cuts at: 4019 5633 8498 4019
Size: 1614 2865 7357
Fragments arranged by size:
7357 2865 1614
SspI AAT'ATT
Cuts at: 5 52 9760 5
Size: 47 9708 2081
Fragments arranged by size:
9708 2081 47
StuI AGG'CCT
Cuts at: 1517 2360 2915 1517
Size: 843 555 10438
Fragments arranged by size:
10438 843 555
StyI C'CwwG_G
Cuts at: 513 1065 2004 2418 4587 5067 5886 6096 6418
Size: 552 939 414 2169 480 819 210 322
Cuts at: 6418 6456 6885 6891 7587 8424 8872 8959 9024
Size: 38 429 6 696 837 448 87 65
Cuts at: 9024 9033 9275 513
Size: 9 242 3074
Fragments arranged by size:
3074 2169 939 837 819 696 552 480
448 429 414 322 242 210 87 65
38 9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5041 5158 5368 5840 6457 6593 7749
Size: 1255 3461 117 210 472 617 136 1156
Cuts at: 7749 9863 9880 10033 10218 10663 325
Size: 2114 17 153 185 445 1498
Fragments arranged by size:
3461 2114 1498 1255 1156 617 472 445
210 185 153 136 117 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2958 3084
Size: 274 80 33 51 1443 525 456 126
Cuts at: 3084 3327 3923 4233 6402 6549 10082 11794 96
Size: 243 596 310 2169 147 3533 1712 138
Fragments arranged by size:
3533 2169 1712 1443 596 525 456 310
274 243 147 138 126 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 3778 3810 5474 5506 11796 11828 2536
Size: 32 1210 32 1664 32 6290 32 2544
Fragments arranged by size:
6290 2544 1664 1210 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11836
XbaI T'CTAG_A
Cuts at: 9172 9172
Size: 11836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3527 4177 4594 5173 5600 6838 8610 9031 3527
Size: 650 417 579 427 1238 1772 421 6332
Fragments arranged by size:
6332 1772 1238 650 579 427 421 417
XhoI C'TCGA_G
Cuts at: 1943 5750 1943
Size: 3807 8029
XmaI C'CCGG_G
Cuts at: 1870 2125 3549 3881 4017 5631 8496 9161 1870
Size: 255 1424 332 136 1614 2865 665 4545
Fragments arranged by size:
4545 2865 1614 1424 665 332 255 136
XmnI GAAnn'nnTTC
Cuts at: 6317 9965 6317
Size: 3648 8188
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7965 9644 276
Size: 53 83 186 936 6431 1679 2468
Fragments arranged by size:
6431 2468 1679 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I Bsu36I EcoRV NruI
PacI RsrII SwaI