Restriction map for
pFN21AE2483
(Circular) (Six-base) MAPSORT of: pFN21AE2483.seq Check: 763 from: 1 to: 5710
pFN21AE2483 5710 bp
With 182 enzymes: SgfI *
September 20, 2013 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2174 2174
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 2732 2767 3773 5157 5425 1632
Size: 857 243 35 1006 1384 268 1917
Fragments arranged by size:
1917 1384 1006 857 268 243 35
AflII C'TTAA_G
Cuts at: 820 1017 2177 820
Size: 197 1160 4353
Fragments arranged by size:
4353 1160 197
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 2106 1166
Size: 940 4770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3002 1184
Size: 1818 3892
AlwNI CAG_nnn'CTG
Cuts at: 2869 3453 5025 2869
Size: 584 1572 3554
Fragments arranged by size:
3554 1572 584
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 2507 3019 3245 3397 5506 1493
Size: 1014 512 226 152 2109 1697
Fragments arranged by size:
2109 1697 1014 512 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 3035 1870
Size: 73 1092 4545
Fragments arranged by size:
4545 1092 73
BamHI G'GATC_C
Cuts at: 2385 3040 3407 2385
Size: 655 367 4688
Fragments arranged by size:
4688 655 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2438 3031 4486 618
Size: 325 217 627 651 593 1455 1842
Fragments arranged by size:
1842 1455 651 627 593 325 217
BanII G_rGCy'C
Cuts at: 1391 3029 1391
Size: 1638 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2143 928
Size: 971 244 4495
Fragments arranged by size:
4495 971 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3901 3935 1903
Size: 34 50 34 1880 34 3678
Fragments arranged by size:
3678 1880 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2181 4321 136
Size: 107 122 71 631 605 509 2140 1525
Fragments arranged by size:
2140 1525 631 605 509 122 107 71
BglII A'GATC_T
Cuts at: 2874 5705 2874
Size: 2831 2879
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 3771 4927 1790
Size: 1981 1156 2573
Fragments arranged by size:
2573 1981 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2234 2681 2899
Size: 416 53 83 399 24 817 447 218
Cuts at: 2899 3441 4399 442
Size: 542 958 1753
Fragments arranged by size:
1753 958 817 542 447 416 399 218
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2734 3014 4370 1239
Size: 1495 280 1356 2579
Fragments arranged by size:
2579 1495 1356 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2588 4373 882
Size: 617 1089 1785 2219
Fragments arranged by size:
2219 1785 1089 617
BsaAI yAC'GTr
Cuts at: 493 1204 2172 2617 3501 493
Size: 711 968 445 884 2702
Fragments arranged by size:
2702 968 884 711 445
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2927 3517 3899 4534
Size: 53 83 186 936 1394 590 382 635
Cuts at: 4534 275
Size: 1451
Fragments arranged by size:
1451 1394 936 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 2839 2869 2885
Size: 30 165 30 1194 30 212 30 16
Cuts at: 2885 2915 1178
Size: 30 3973
Fragments arranged by size:
3973 1194 212 165 30 30 30 30
30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2135 2157 1186
Size: 868 81 22 4739
Fragments arranged by size:
4739 868 81 22
BseYI C'CCAG_C
Cuts at: 2057 2360 3001 4913 5409 2057
Size: 303 641 1912 496 2358
Fragments arranged by size:
2358 1912 641 496 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2152 2635 1163
Size: 573 416 483 4238
Fragments arranged by size:
4238 573 483 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
BsmBI CGTCTCn'nnnn_
Cuts at: 2198 2918 2198
Size: 720 4990
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3029 3771 3856 4927 1391
Size: 399 1239 742 85 1071 2174
Fragments arranged by size:
2174 1239 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 2458 3599 5329 2458
Size: 1141 1730 2839
Fragments arranged by size:
2839 1730 1141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2174 2406 3065 844
Size: 1330 232 659 3489
Fragments arranged by size:
3489 1330 659 232
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2244 2374 4205 4379 65
Size: 1183 996 130 1831 174 1396
Fragments arranged by size:
1831 1396 1183 996 174 130
BsrFI r'CCGG_y
Cuts at: 1700 1810 4354 4541 1700
Size: 110 2544 187 2869
Fragments arranged by size:
2869 2544 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 2040 1925
Size: 115 5595
BssSI C'ACGA_G
Cuts at: 2632 3770 4782 2632
Size: 1138 1012 3560
Fragments arranged by size:
3560 1138 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2108 2449 2704 2108
Size: 341 255 5114
Fragments arranged by size:
5114 341 255
BstYI r'GATC_y
Cuts at: 1972 2385 2874 3040 3407 3792 3809 5261 5347
Size: 413 489 166 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 489 413 385 367 346 166
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5158
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2965 487
Size: 683 56 1739 3232
Fragments arranged by size:
3232 1739 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2451 3157
Size: 54 1425 50 275 18 195 426 706
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 706 426 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2786 3640 1353
Size: 1433 854 3423
Fragments arranged by size:
3423 1433 854
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 2732 2734 2767 3014 3773 4370
Size: 393 857 243 2 33 247 759 597
Cuts at: 4370 5157 5425 1239
Size: 787 268 1524
Fragments arranged by size:
1524 857 787 759 597 393 268 247
243 33 2
EcoO109I rG'GnC_Cy
Cuts at: 2308 2308
Size: 5710
EcoRI G'AATT_C
Cuts at: 2507 3019 3397 2507
Size: 512 378 4820
Fragments arranged by size:
4820 512 378
EcoRV GAT'ATC
Cuts at: 2080 2080
Size: 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 5710
FspI TGC'GCA
Cuts at: 2951 4216 2951
Size: 1265 4445
FspAI rTGC'GCAy
Cuts at: 2951 2951
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 4857 1678
Size: 3179 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2133 2165 2885 2917 4351
Size: 32 135 32 561 32 720 32 1434
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1434 720 561 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2584 3054 3300 669
Size: 988 281 646 470 246 3079
Fragments arranged by size:
3079 988 646 470 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 10
Size: 54 1425 343 621 3267
Fragments arranged by size:
3267 1425 621 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 3668 4027 4186 518
Size: 648 940 1562 359 159 2042
Fragments arranged by size:
2042 1562 940 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2787 3126 3803 4951 5196 1606
Size: 1181 339 677 1148 245 2120
Fragments arranged by size:
2120 1181 1148 677 339 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5158
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2582 3068 4613 1132
Size: 97 1353 486 1545 2229
Fragments arranged by size:
2229 1545 1353 486 97
PasI CC'CwG_GG
Cuts at: 1348 2704 1348
Size: 1356 4354
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PfoI T'CCnGG_A
Cuts at: 2310 2310
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PmlI CAC'GTG
Cuts at: 2172 2172
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 5710
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2874 3062 830
Size: 2044 188 3478
Fragments arranged by size:
3478 2044 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2787 2787
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2308 2308
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2488 2870 2961 3058 4193 4874 5065
Size: 219 1443 382 91 97 1135 681 191
Cuts at: 5065 826
Size: 1471
Fragments arranged by size:
1471 1443 1135 681 382 219 191 97
91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 3814 4715 4977 820
Size: 197 926 234 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 262 234 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 4753
StyI C'CwwG_G
Cuts at: 513 1065 3149 513
Size: 552 2084 3074
Fragments arranged by size:
3074 2084 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2374 3737 3754 3907 4092 4537 325
Size: 1255 794 1363 17 153 185 445 1498
Fragments arranged by size:
1498 1363 1255 794 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 2474 3839 2474
Size: 1365 4345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI