Restriction map for
pFN21AE2481
(Circular) (Six-base) MAPSORT of: pFN21AE2481.seq Check: 7816 from: 1 to: 6121
pFN21AE2481 6121 bp
With 182 enzymes: SgfI *
August 14, 2013 14:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2855 3931 278
Size: 53 83 186 936 1319 1076 2468
Fragments arranged by size:
2468 1319 1076 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2051 3464 1937
Size: 114 1413 4594
Fragments arranged by size:
4594 1413 114
Acc65I G'GTAC_C
Cuts at: 3442 3442
Size: 6121
AclI AA'CG_TT
Cuts at: 1563 3073 4248 4621 1563
Size: 1510 1175 373 3063
Fragments arranged by size:
3063 1510 1175 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4184 5568 5836 1632
Size: 2552 1384 268 1917
Fragments arranged by size:
2552 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 4850 4850
Size: 6121
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3002 1184
Size: 1818 4303
AlwNI CAG_nnn'CTG
Cuts at: 2017 3864 5436 2017
Size: 1847 1572 2702
Fragments arranged by size:
2702 1847 1572
ApaI G_GGCC'C
Cuts at: 2697 2697
Size: 6121
ApaLI G'TGCA_C
Cuts at: 2996 4178 5334 2996
Size: 1182 1156 3783
Fragments arranged by size:
3783 1182 1156
ApoI r'AATT_y
Cuts at: 1493 2129 2792 3430 3656 3808 5917 1493
Size: 636 663 638 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 638 636 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 2181 2457 2603 3057 3446 1870
Size: 73 181 57 276 146 454 389 4545
Fragments arranged by size:
4545 454 389 276 181 146 73 57
BamHI G'GATC_C
Cuts at: 2468 3401 3451 3818 2468
Size: 933 50 367 4771
Fragments arranged by size:
4771 933 367 50
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2954 3200 3442 4897
Size: 325 217 627 387 780 246 242 1455
Cuts at: 4897 618
Size: 1842
Fragments arranged by size:
1842 1455 780 627 387 325 246 242
217
BanII G_rGCy'C
Cuts at: 1391 2228 2636 2697 3373 3440 1391
Size: 837 408 61 676 67 4072
Fragments arranged by size:
4072 837 676 408 67 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 2424 2517 2857 3150 928
Size: 971 118 407 93 340 293 3899
Fragments arranged by size:
3899 971 407 340 293 118 93
BbvCI CC'TCA_GC
Cuts at: 2307 2307
Size: 6121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4312 4346 1903
Size: 34 2375 34 3678
Fragments arranged by size:
3678 2375 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6121
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 4732 136
Size: 107 122 71 631 605 599 2461 1525
Fragments arranged by size:
2461 1525 631 605 599 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 2589 3538 2589
Size: 949 5172
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2697 3000 4182 5338 1790
Size: 387 520 303 1182 1156 2573
Fragments arranged by size:
2573 1182 1156 520 387 303
BmgBI CAC'GTC
Cuts at: 1318 2160 1318
Size: 842 5279
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 3852 4810
Size: 416 53 83 399 24 762 1673 958
Cuts at: 4810 442
Size: 1753
Fragments arranged by size:
1753 1673 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2762 4781 1239
Size: 1523 2019 2579
Fragments arranged by size:
2579 2019 1523
Bpu10I CC'TnA_GC
Cuts at: 2307 2348 2307
Size: 41 6080
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3146 4246 5111 5409 3146
Size: 1100 865 298 3858
Fragments arranged by size:
3858 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2147 2289 2887 3109 4784 882
Size: 617 648 142 598 222 1675 2219
Fragments arranged by size:
2219 1675 648 617 598 222 142
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2852 3928 4310 4945
Size: 53 83 186 936 1319 1076 382 635
Cuts at: 4945 275
Size: 1451
Fragments arranged by size:
1451 1319 1076 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3365 3398 4554 4952 5226 5373 5784 3365
Size: 33 1156 398 274 147 411 3702
Fragments arranged by size:
3702 1156 411 398 274 147 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2380 2410 1178
Size: 30 165 30 741 30 206 30 4889
Fragments arranged by size:
4889 741 206 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2050 2153 2162 2165 2879 3164 1186
Size: 864 103 9 3 714 285 4143
Fragments arranged by size:
4143 864 714 285 103 9 3
BseYI C'CCAG_C
Cuts at: 2312 2342 2448 2555 2619 5324 5820 2312
Size: 30 106 107 64 2705 496 2613
Fragments arranged by size:
2705 2613 496 107 106 64 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2885 1163
Size: 573 363 786 4399
Fragments arranged by size:
4399 786 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2675 3571 4332 5360 1540
Size: 275 186 674 896 761 1028 2301
Fragments arranged by size:
2301 1028 896 761 674 275 186
BsiHKAI G_wGCw'C
Cuts at: 3000 4182 4267 5338 3000
Size: 1182 85 1071 3783
Fragments arranged by size:
3783 1182 1071 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2228 2636 2697 3000 3373 3440
Size: 399 387 51 408 61 303 373 67
Cuts at: 3440 4182 4267 5338 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 387 373 303
85 67 61 51
BspEI T'CCGG_A
Cuts at: 3398 5784 3398
Size: 2386 3735
BspHI T'CATG_A
Cuts at: 4010 5740 4010
Size: 1730 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2002 2835 3476 844
Size: 1158 833 641 3489
Fragments arranged by size:
3489 1158 833 641
BsrBI CCG'CTC
Cuts at: 1394 1823 2277 4008 1394
Size: 429 454 1731 3507
Fragments arranged by size:
3507 1731 454 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2705 4616 4790 65
Size: 1183 1457 1911 174 1396
Fragments arranged by size:
1911 1457 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2651 3270 4765 4952 1700
Size: 110 210 631 619 1495 187 2869
Fragments arranged by size:
2869 1495 631 619 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 2292 2845 3149 4181 5193 2292
Size: 553 304 1032 1012 3220
Fragments arranged by size:
3220 1032 1012 553 304
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2017 2995 794
Size: 1223 978 3920
Fragments arranged by size:
3920 1223 978
BstBI TT'CG_AA
Cuts at: 2684 4925 2684
Size: 2241 3880
BstEII G'GTnAC_C
Cuts at: 1184 3310 1184
Size: 2126 3995
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 2539 2506
Size: 33 6088
BstYI r'GATC_y
Cuts at: 1972 2468 3342 3401 3451 3818 4203 4220 5672
Size: 496 874 59 50 367 385 17 1452
Cuts at: 5672 5758 5770 6116 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 874 496 385 367 346 86
59 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2423 2423
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2004 2919 513
Size: 552 939 915 3715
Fragments arranged by size:
3715 939 915 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2188 2583 487
Size: 683 56 962 395 4025
Fragments arranged by size:
4025 962 683 395 56
BtsI GCAGTG_nn'
Cuts at: 2032 2735 3637 4431 4451 2032
Size: 703 902 794 20 3702
Fragments arranged by size:
3702 902 794 703 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2922 3111 3568
Size: 54 1425 50 275 18 1092 189 457
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1092 889 457 275 189 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2488 2694 2709 3182 3228 4051 1353
Size: 1135 206 15 473 46 823 3423
Fragments arranged by size:
3423 1135 823 473 206 46 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2837 4708 5082 5228 387
Size: 1064 138 1248 1871 374 146 1280
Fragments arranged by size:
1871 1280 1248 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 4184 4781 5568 5836 1239
Size: 393 1130 1422 597 787 268 1524
Fragments arranged by size:
1524 1422 1130 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2014 2014
Size: 6121
EcoRI G'AATT_C
Cuts at: 2129 2792 3430 3808 2129
Size: 663 638 378 4442
Fragments arranged by size:
4442 663 638 378
FspI TGC'GCA
Cuts at: 4627 4627
Size: 6121
HaeII r_GCGC'y
Cuts at: 1678 2410 5268 1678
Size: 732 2858 2531
Fragments arranged by size:
2858 2531 732
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2672 2704 2754
Size: 32 135 32 572 32 496 32 50
Cuts at: 2754 2786 3339 3371 4762 4794 4836 4868 1373
Size: 32 553 32 1391 32 42 32 2626
Fragments arranged by size:
2626 1391 572 553 496 135 50 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3465 3711 669
Size: 988 281 1527 246 3079
Fragments arranged by size:
3079 1527 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 3446 3446
Size: 6121
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 2605 5235 5419 1305
Size: 1110 190 2630 184 2007
Fragments arranged by size:
2630 2007 1110 190 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4299
Fragments arranged by size:
4299 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4079 4438 4597 518
Size: 648 2913 359 159 2042
Fragments arranged by size:
2913 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2506 3121 3537 4214 5362 5607 1606
Size: 900 615 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 900 677 615 416 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NruI TCG'CGA
Cuts at: 2677 2677
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2269 3359 1348
Size: 921 1090 4110
Fragments arranged by size:
4110 1090 921
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3301 1108
Size: 2193 3928
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2081 2113 4190 4222 5703 5735 2081
Size: 32 2077 32 1481 32 2467
Fragments arranged by size:
2467 2077 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2014
Size: 6121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PspOMI G'GGCC_C
Cuts at: 2693 2693
Size: 6121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2556 2588 2556
Size: 32 6089
PstI C_TGCA'G
Cuts at: 830 2991 3385 3473 830
Size: 2161 394 88 3478
Fragments arranged by size:
3478 2161 394 88
PvuI CG_AT'CG
Cuts at: 2001 2675 2001
Size: 674 5447
PvuII CAG'CTG
Cuts at: 3121 3121
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 2694 2694
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2052 2987 3381 3469 4604 5285 5476
Size: 219 1007 935 394 88 1135 681 191
Cuts at: 5476 826
Size: 1471
Fragments arranged by size:
1471 1135 1007 935 681 394 219 191
88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 2126 2183 2459 3448 1872
Size: 254 57 276 989 4545
Fragments arranged by size:
4545 989 276 254 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3161 4225 5126 5388 820
Size: 197 926 1218 1064 901 262 1553
Fragments arranged by size:
1553 1218 1064 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 2874 3029 1517
Size: 1357 155 4609
Fragments arranged by size:
4609 1357 155
StyI C'CwwG_G
Cuts at: 513 1065 2004 3560 513
Size: 552 939 1556 3074
Fragments arranged by size:
3074 1556 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2440 3188 4148 4165 4318 4503 4948
Size: 1255 860 748 960 17 153 185 445
Cuts at: 4948 325
Size: 1498
Fragments arranged by size:
1498 1255 960 860 748 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4367 6079
Size: 274 80 33 51 1443 855 1535 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
1712 1535 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 6081 6113 2380
Size: 32 3669 32 2388
Fragments arranged by size:
3669 2388 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2158 1316
Size: 842 5279
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 2124 2181 2457 3446 1870
Size: 254 57 276 989 4545
Fragments arranged by size:
4545 989 276 254 57
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2853 3929 276
Size: 53 83 186 936 1319 1076 2468
Fragments arranged by size:
2468 1319 1076 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI