Restriction map for
pFN21AE2478
(Circular) (Six-base) MAPSORT of: pFN21AE2478.seq Check: 6204 from: 1 to: 7618
pFN21AE2478 7618 bp
With 182 enzymes: SgfI *
July 26, 2013 12:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2510 2510
Size: 7618
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5428 278
Size: 53 83 186 936 3892 2468
Fragments arranged by size:
3892 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4961 1937
Size: 3024 4594
Acc65I G'GTAC_C
Cuts at: 4939 4939
Size: 7618
AclI AA'CG_TT
Cuts at: 1563 5745 6118 1563
Size: 4182 373 3063
Fragments arranged by size:
4182 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3432 4104 5681 7065 7333 1632
Size: 1800 672 1577 1384 268 1917
Fragments arranged by size:
1917 1800 1577 1384 672 268
AfeI AGC'GCT
Cuts at: 4651 4651
Size: 7618
AflII C'TTAA_G
Cuts at: 820 1017 4763 820
Size: 197 3746 3675
Fragments arranged by size:
3746 3675 197
AflIII A'CryG_T
Cuts at: 1203 2858 2963 6517 7494 1203
Size: 1655 105 3554 977 1327
Fragments arranged by size:
3554 1655 1327 977 105
AgeI A'CCGG_T
Cuts at: 6449 6449
Size: 7618
AhdI GACnn_n'nnGTC
Cuts at: 6347 6347
Size: 7618
AleI CACnn'nnGTG
Cuts at: 1166 2685 2810 3359 1166
Size: 1519 125 549 5425
Fragments arranged by size:
5425 1519 549 125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7618
AlwNI CAG_nnn'CTG
Cuts at: 5361 6933 5361
Size: 1572 6046
ApaLI G'TGCA_C
Cuts at: 5675 6831 5675
Size: 1156 6462
ApoI r'AATT_y
Cuts at: 1493 4253 4927 5153 5305 7414 1493
Size: 2760 674 226 152 2109 1697
Fragments arranged by size:
2760 2109 1697 674 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6172 160
Size: 872 5140 1606
Fragments arranged by size:
5140 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 3617 4845 4943 1870
Size: 73 87 1587 1228 98 4545
Fragments arranged by size:
4545 1587 1228 98 87 73
BamHI G'GATC_C
Cuts at: 4948 5315 4948
Size: 367 7251
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2673 4939 6394 618
Size: 325 217 627 886 2266 1455 1842
Fragments arranged by size:
2266 1842 1455 886 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2286 3348 3721 4937 1391
Size: 895 1062 373 1216 4072
Fragments arranged by size:
4072 1216 1062 895 373
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2966 4716 928
Size: 971 1067 1750 3830
Fragments arranged by size:
3830 1750 1067 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3007 3041 3901 3935 4083 4117 4447
Size: 34 1070 34 860 34 148 34 330
Cuts at: 4447 4481 5809 5843 1903
Size: 34 1328 34 3678
Fragments arranged by size:
3678 1328 1070 860 330 148 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2893 5510 6720 2893
Size: 2617 1210 3791
Fragments arranged by size:
3791 2617 1210
BclI T'GATC_A
Cuts at: 1548 3007 1548
Size: 1459 6159
BfrBI ATG'CAT
Cuts at: 4739 4739
Size: 7618
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6229 136
Size: 107 122 71 631 605 4557 1525
Fragments arranged by size:
4557 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4479 7613 4479
Size: 3134 4484
BlpI GC'TnA_GC
Cuts at: 3606 5035 3606
Size: 1429 6189
Bme1580I G_kGCm'C
Cuts at: 1790 2155 4066 4073 5679 6835 1790
Size: 365 1911 7 1606 1156 2573
Fragments arranged by size:
2573 1911 1606 1156 365 7
BmgBI CAC'GTC
Cuts at: 1318 3925 1318
Size: 2607 5011
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4226 4321 4380
Size: 416 53 83 399 24 2809 95 59
Cuts at: 4380 5349 6307 442
Size: 969 958 1753
Fragments arranged by size:
2809 1753 969 958 416 399 95 83
59 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2804 2930 3884 4585 6278 1239
Size: 1184 381 126 954 701 1693 2579
Fragments arranged by size:
2579 1693 1184 954 701 381 126
Bpu10I CC'TnA_GC
Cuts at: 2528 3812 2528
Size: 1284 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2783 5743 6608 6906 2783
Size: 2960 865 298 3495
Fragments arranged by size:
3495 2960 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4900 6281 882
Size: 617 3401 1381 2219
Fragments arranged by size:
3401 2219 1381 617
BsaAI yAC'GTr
Cuts at: 493 1204 3679 4428 4675 5409 493
Size: 711 2475 749 247 734 2702
Fragments arranged by size:
2702 2475 749 734 711 247
BsaBI GATnn'nnATC
Cuts at: 7612 7612
Size: 7618
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 5425 5807 6442
Size: 53 83 186 936 648 3244 382 635
Cuts at: 6442 275
Size: 1451
Fragments arranged by size:
3244 1451 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6051 6449 6723 6870 7281 6051
Size: 398 274 147 411 6388
Fragments arranged by size:
6388 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 2283 2313 1178
Size: 30 165 30 798 30 52 30 6483
Fragments arranged by size:
6483 798 165 52 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2767 3417 3483 4131 1186
Size: 1581 650 66 648 4673
Fragments arranged by size:
4673 1581 650 648 66
BseYI C'CCAG_C
Cuts at: 4357 4408 6821 7317 4357
Size: 51 2413 496 4658
Fragments arranged by size:
4658 2413 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2623 2734 3244 3365 1163
Size: 573 887 111 510 121 5416
Fragments arranged by size:
5416 887 573 510 121 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5068 5829 6857 1540
Size: 275 186 3067 761 1028 2301
Fragments arranged by size:
3067 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2219 2286 3110 5679 5764 6835 2219
Size: 67 824 2569 85 1071 3002
Fragments arranged by size:
3002 2569 1071 824 85 67
BsmI GAATG_Cn'
Cuts at: 2505 3287 3435 5129 5222 2505
Size: 782 148 1694 93 4901
Fragments arranged by size:
4901 1694 782 148 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 7618
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2219 2286 3110 3348 3721 4066
Size: 399 365 64 67 824 238 373 345
Cuts at: 4066 4073 4937 5679 5764 6835 1391
Size: 7 864 742 85 1071 2174
Fragments arranged by size:
2174 1071 864 824 742 399 373 365
345 238 85 67 64 7
BspEI T'CCGG_A
Cuts at: 7281 7281
Size: 7618
BspHI T'CATG_A
Cuts at: 2365 5507 7237 2365
Size: 3142 1730 2746
Fragments arranged by size:
3142 2746 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2510 3241 4574 4973 844
Size: 1666 731 1333 399 3489
Fragments arranged by size:
3489 1666 1333 731 399
BsrBI CCG'CTC
Cuts at: 1394 1823 2514 5505 1394
Size: 429 691 2991 3507
Fragments arranged by size:
3507 2991 691 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3001 6113 6287 65
Size: 1183 1753 3112 174 1396
Fragments arranged by size:
3112 1753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6262 6449 1700
Size: 110 4452 187 2869
Fragments arranged by size:
4452 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7618
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7618
BssSI C'ACGA_G
Cuts at: 5678 6690 5678
Size: 1012 6606
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7618
BstBI TT'CG_AA
Cuts at: 6422 6422
Size: 7618
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7618
BstXI CCAn_nnnn'nTGG
Cuts at: 2812 2954 2812
Size: 142 7476
BstYI r'GATC_y
Cuts at: 1972 4479 4948 5315 5700 5717 7169 7255 7267
Size: 2507 469 367 385 17 1452 86 12
Cuts at: 7267 7613 1972
Size: 346 1977
Fragments arranged by size:
2507 1977 1452 469 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3741 3741
Size: 7618
BtgI C'CryG_G
Cuts at: 513 1065 2004 2121 2659 513
Size: 552 939 117 538 5472
Fragments arranged by size:
5472 939 552 538 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4573 487
Size: 683 56 3347 3532
Fragments arranged by size:
3532 3347 683 56
BtsI GCAGTG_nn'
Cuts at: 2075 2258 2917 3382 5134 5928 5948 2075
Size: 183 659 465 1752 794 20 3745
Fragments arranged by size:
3745 1752 794 659 465 183 20
ClaI AT'CG_AT
Cuts at: 1554 2265 1554
Size: 711 6907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2906 2941 529
Size: 35 2342 35 5206
Fragments arranged by size:
5206 2342 35 35
DraI TTT'AAA
Cuts at: 3064 4922 3064
Size: 1858 5760
DraIII CAC_nnn'GTG
Cuts at: 3371 4422 3371
Size: 1051 6567
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6625 809
Size: 507 5309 1802
Fragments arranged by size:
5309 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2622 3724 5065
Size: 54 1425 50 275 18 792 1102 1341
Cuts at: 5065 5954 7583 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1341 1102 889 792 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5065 1537
Size: 275 3253 4090
Fragments arranged by size:
4090 3253 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 3451 3490 4540 5548 1353
Size: 1161 937 39 1050 1008 3423
Fragments arranged by size:
3423 1161 1050 1008 937 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6205 6579 6725 387
Size: 1064 138 4616 374 146 1280
Fragments arranged by size:
4616 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2284 2284
Size: 7618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2804 2930 3432 3884 4104 4585
Size: 393 791 381 126 502 452 220 481
Cuts at: 4585 5681 6278 7065 7333 1239
Size: 1096 597 787 268 1524
Fragments arranged by size:
1524 1096 791 787 597 502 481 452
393 381 268 220 126
EcoO109I rG'GnC_Cy
Cuts at: 2524 2575 3120 3462 4666 2524
Size: 51 545 342 1204 5476
Fragments arranged by size:
5476 1204 545 342 51
EcoRI G'AATT_C
Cuts at: 4927 5305 4927
Size: 378 7240
FspI TGC'GCA
Cuts at: 6124 6124
Size: 7618
HaeII r_GCGC'y
Cuts at: 1678 4456 4653 6765 1678
Size: 2778 197 2112 2531
Fragments arranged by size:
2778 2531 2112 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 2476 2508 2628
Size: 32 135 32 711 32 161 32 120
Cuts at: 2628 2660 2928 2960 3068 3092 3100 3124 4854
Size: 32 268 32 108 24 8 24 1730
Cuts at: 4854 4886 6259 6291 6333 6365 1373
Size: 32 1373 32 42 32 2626
Fragments arranged by size:
2626 1730 1373 711 268 161 135 120
108 42 32 32 32 32 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2437 2869 4016 4962 5208 669
Size: 988 281 499 432 1147 946 246 3079
Fragments arranged by size:
3079 1147 988 946 499 432 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7618
HpaI GTT'AAC
Cuts at: 5208 5208
Size: 7618
KpnI G_GTAC'C
Cuts at: 4943 4943
Size: 7618
MfeI C'AATT_G
Cuts at: 5217 5217
Size: 7618
MluI A'CGCG_T
Cuts at: 7494 7494
Size: 7618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2914 2931 6732 6916 1305
Size: 1609 17 3801 184 2007
Fragments arranged by size:
3801 2007 1609 184 17
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 10
Size: 54 1425 343 792 5004
Fragments arranged by size:
5004 1425 792 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 2685 2745 2810 3359 5576 5935
Size: 648 1264 255 60 65 549 2217 359
Cuts at: 5935 6094 518
Size: 159 2042
Fragments arranged by size:
2217 2042 1264 648 549 359 255 159
65 60
MspA1I CmG'CkG
Cuts at: 1606 2123 2718 3135 5034 5711 6859 7104 1606
Size: 517 595 417 1899 677 1148 245 2120
Fragments arranged by size:
2120 1899 1148 677 595 517 417 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2659 513
Size: 552 939 655 5472
Fragments arranged by size:
5472 939 655 552
NdeI CA'TA_TG
Cuts at: 387 4558 4681 387
Size: 4171 123 3324
Fragments arranged by size:
4171 3324 123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7618
NotI GC'GGCC_GC
Cuts at: 5065 5065
Size: 7618
NsiI A_TGCA'T
Cuts at: 4741 4741
Size: 7618
NspI r_CATG'y
Cuts at: 1132 1229 2967 3693 4976 6521 1132
Size: 97 1738 726 1283 1545 2229
Fragments arranged by size:
2229 1738 1545 1283 726 97
PasI CC'CwG_GG
Cuts at: 1348 3296 4066 1348
Size: 1948 770 4900
Fragments arranged by size:
4900 1948 770
PciI A'CATG_T
Cuts at: 2963 6517 2963
Size: 3554 4064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3097 1108
Size: 1989 5629
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 7618
PmeI GTTT'AAAC
Cuts at: 4922 4922
Size: 7618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5687 5719 7200 7232 5687
Size: 32 1481 32 6073
Fragments arranged by size:
6073 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3120 4666 3120
Size: 1546 6072
PshAI GACnn'nnGTC
Cuts at: 1613 2912 1613
Size: 1299 6319
PsiI TTA'TAA
Cuts at: 5188 5188
Size: 7618
PstI C_TGCA'G
Cuts at: 830 2924 4036 4970 830
Size: 2094 1112 934 3478
Fragments arranged by size:
3478 2094 1112 934
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7618
PvuII CAG'CTG
Cuts at: 2718 3135 2718
Size: 417 7201
SacI G_AGCT'C
Cuts at: 2286 2286
Size: 7618
SacII CC_GC'GG
Cuts at: 2124 2124
Size: 7618
SalI G'TCGA_C
Cuts at: 1936 4960 1936
Size: 3024 4594
SapI GCTCTTCn'nnn_
Cuts at: 3490 3490
Size: 7618
SbfI CC_TGCA'GG
Cuts at: 4970 4970
Size: 7618
ScaI AGT'ACT
Cuts at: 5866 7578 5866
Size: 1712 5906
SexAI A'CCwGG_T
Cuts at: 4018 4018
Size: 7618
SfcI C'TryA_G
Cuts at: 826 1045 2554 2920 4032 4966 6101 6782 6973
Size: 219 1509 366 1112 934 1135 681 191
Cuts at: 6973 826
Size: 1471
Fragments arranged by size:
1509 1471 1135 1112 934 681 366 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7618
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7618
SmaI CCC'GGG
Cuts at: 1872 2032 4945 1872
Size: 160 2913 4545
Fragments arranged by size:
4545 2913 160
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 4763 5722 6623 6885 820
Size: 197 926 819 2001 959 901 262 1553
Fragments arranged by size:
2001 1553 959 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 3679 5409 493
Size: 3186 1730 2702
Fragments arranged by size:
3186 2702 1730
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7618
SphI G_CATG'C
Cuts at: 1132 4976 1132
Size: 3844 3774
SspI AAT'ATT
Cuts at: 5 52 2317 4028 5542 5
Size: 47 2265 1711 1514 2081
Fragments arranged by size:
2265 2081 1711 1514 47
StuI AGG'CCT
Cuts at: 1517 2160 2608 4122 1517
Size: 643 448 1514 5013
Fragments arranged by size:
5013 1514 643 448
StyI C'CwwG_G
Cuts at: 513 1065 2004 2155 2236 2579 2659 5057 513
Size: 552 939 151 81 343 80 2398 3074
Fragments arranged by size:
3074 2398 939 552 343 151 81 80
SwaI ATTT'AAAT
Cuts at: 3064 3064
Size: 7618
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2215 2661 3215 5645 5662 5815 6000
Size: 1255 635 446 554 2430 17 153 185
Cuts at: 6000 6445 325
Size: 445 1498
Fragments arranged by size:
2430 1498 1255 635 554 446 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 4827 5864
Size: 274 80 33 51 1443 753 2097 1037
Cuts at: 5864 7576 96
Size: 1712 138
Fragments arranged by size:
2097 1712 1443 1037 753 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7578 7610 7578
Size: 32 7586
Tth111I GACn'n_nGTC
Cuts at: 1316 2777 1316
Size: 1461 6157
XbaI T'CTAG_A
Cuts at: 4954 4954
Size: 7618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 2812 2993 3293 4085 4253 2666
Size: 146 181 300 792 168 6031
Fragments arranged by size:
6031 792 300 181 168 146
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7618
XmaI C'CCGG_G
Cuts at: 1870 2030 4943 1870
Size: 160 2913 4545
Fragments arranged by size:
4545 2913 160
XmnI GAAnn'nnTTC
Cuts at: 4820 5747 4820
Size: 927 6691
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5426 276
Size: 53 83 186 936 3892 2468
Fragments arranged by size:
3892 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SfiI SfoI SrfI