Restriction map for
pFN21AE2476
(Circular) (Six-base) MAPSORT of: pFN21AE2476.seq Check: 7828 from: 1 to: 6205
pFN21AE2476 6205 bp
With 182 enzymes: SgfI *
June 18, 2013 12:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2596 2810 2596
Size: 214 5991
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2982 3548 1937
Size: 1045 566 4594
Fragments arranged by size:
4594 1045 566
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2566 2954 3083 3485 4268 5652 5920 1632
Size: 934 388 129 402 783 1384 268 1917
Fragments arranged by size:
1917 1384 934 783 402 388 268 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 2263 5104 6081 1203
Size: 1060 2841 977 1327
Fragments arranged by size:
2841 1327 1060 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2733 2765 2733
Size: 32 6173
AlwNI CAG_nnn'CTG
Cuts at: 3948 5520 3948
Size: 1572 4633
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 2792 3154 3514 3740 3892 6001 1493
Size: 1299 362 360 226 152 2109 1697
Fragments arranged by size:
2109 1697 1299 362 360 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 3211 3292 3530 1870
Size: 73 99 1169 81 238 4545
Fragments arranged by size:
4545 1169 238 99 81 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2658 2691 2658
Size: 33 6172
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3526 4981 618
Size: 325 217 627 1739 1455 1842
Fragments arranged by size:
1842 1739 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3218 3524 1391
Size: 1827 306 4072
Fragments arranged by size:
4072 1827 306
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2322 2409 3071 3401 3411 928
Size: 971 423 87 662 330 10 3722
Fragments arranged by size:
3722 971 662 423 330 87 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3273 3307 4396 4430 1903
Size: 34 50 34 1252 34 1089 34 3678
Fragments arranged by size:
3678 1252 1089 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 2896 1548
Size: 1348 4857
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 4816 136
Size: 107 122 71 631 605 430 2714 1525
Fragments arranged by size:
2714 1525 631 605 430 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 3622 3622
Size: 6205
Bme1580I G_kGCm'C
Cuts at: 1790 2033 4266 5422 1790
Size: 243 2233 1156 2573
Fragments arranged by size:
2573 2233 1156 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4894 442
Size: 416 53 83 399 24 2519 958 1753
Fragments arranged by size:
2519 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4865 1239
Size: 3626 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4330 5195 5493 4330
Size: 865 298 5042
Fragments arranged by size:
5042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4012 4394 5029 275
Size: 53 83 186 936 2479 382 635 1451
Fragments arranged by size:
2479 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3185 4638 5036 5310 5457 5868 3185
Size: 1453 398 274 147 411 3522
Fragments arranged by size:
3522 1453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2733 2763 3191 3221 1178
Size: 30 165 30 1330 30 428 30 4162
Fragments arranged by size:
4162 1330 428 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 3414 1186
Size: 1387 841 3977
Fragments arranged by size:
3977 1387 841
BseYI C'CCAG_C
Cuts at: 2768 5408 5904 2768
Size: 2640 496 3069
Fragments arranged by size:
3069 2640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2624 1163
Size: 573 303 585 4744
Fragments arranged by size:
4744 585 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2293 4266 4351 5422 2043
Size: 250 1973 85 1071 2826
Fragments arranged by size:
2826 1973 1071 250 85
BsmI GAATG_Cn'
Cuts at: 3417 3716 3809 3417
Size: 299 93 5813
Fragments arranged by size:
5813 299 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2043 2293 3218 3524 4266 4351
Size: 399 243 10 250 925 306 742 85
Cuts at: 4351 5422 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 925 742 399 306 250 243
85 10
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 2788 4094 5824 2788
Size: 1306 1730 3169
Fragments arranged by size:
3169 1730 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2596 2810 3560 844
Size: 1752 214 750 3489
Fragments arranged by size:
3489 1752 750 214
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 2780 4700 4874 65
Size: 1183 1323 209 1920 174 1396
Fragments arranged by size:
1920 1396 1323 1183 209 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 4849 5036 1700
Size: 110 295 2744 187 2869
Fragments arranged by size:
2869 2744 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 2203 5009 2203
Size: 2806 3399
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 2509 2509
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2326 3535 3902 4287 4304 5756 5842 5854
Size: 354 1209 367 385 17 1452 86 12
Cuts at: 5854 6200 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1209 385 367 354 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2424 2424
Size: 6205
BtgI C'CryG_G
Cuts at: 513 1065 2631 2695 3437 513
Size: 552 1566 64 742 3281
Fragments arranged by size:
3281 1566 742 552 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 3721 4515 4535 3721
Size: 794 20 5391
Fragments arranged by size:
5391 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DraIII CAC_nnn'GTG
Cuts at: 2725 2725
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3652 4541 6170
Size: 54 1425 50 275 18 1822 889 1629
Cuts at: 6170 8
Size: 43
Fragments arranged by size:
1822 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2573 4135 1353
Size: 1220 1562 3423
Fragments arranged by size:
3423 1562 1220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2566 2954 3083 3485 4268 4865 5652
Size: 393 934 388 129 402 783 597 787
Cuts at: 5652 5920 1239
Size: 268 1524
Fragments arranged by size:
1524 934 787 783 597 402 393 388
268 129
EcoO109I rG'GnC_Cy
Cuts at: 2563 3356 2563
Size: 793 5412
EcoRI G'AATT_C
Cuts at: 2792 3514 3892 2792
Size: 722 378 5105
Fragments arranged by size:
5105 722 378
FseI GG_CCGG'CC
Cuts at: 2109 2109
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 5352 1678
Size: 3674 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2396 2428 3240 3272 4846
Size: 32 135 32 824 32 812 32 1574
Cuts at: 4846 4878 4920 4952 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1574 824 812 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2261 2983 3178 3549 3795 669
Size: 988 281 323 722 195 371 246 3079
Fragments arranged by size:
3079 988 722 371 323 281 246 195
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3183 5319 5503 1305
Size: 1878 2136 184 2007
Fragments arranged by size:
2136 2007 1878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4383
Fragments arranged by size:
4383 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 2225 2298 3341 4163 4522 4681
Size: 648 991 68 73 1043 822 359 159
Cuts at: 4681 518
Size: 2042
Fragments arranged by size:
2042 1043 991 822 648 359 159 73
68
MspA1I CmG'CkG
Cuts at: 1606 2768 3621 4298 5446 5691 1606
Size: 1162 853 677 1148 245 2120
Fragments arranged by size:
2120 1162 1148 853 677 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5653
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3240 3563 5108 1132
Size: 97 2011 323 1545 2229
Fragments arranged by size:
2229 2011 1545 323 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2597 1108
Size: 1489 4716
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2733 2765 4274 4306 5787 5819 2733
Size: 32 1509 32 1481 32 3119
Fragments arranged by size:
3119 1509 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3356 3356
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PstI C_TGCA'G
Cuts at: 830 2116 3557 830
Size: 1286 1441 3478
Fragments arranged by size:
3478 1441 1286
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2768 2768
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 2981 3547 1936
Size: 1045 566 4594
Fragments arranged by size:
4594 1045 566
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2112 2240 3022 3553 4688 5369 5560
Size: 219 1067 128 782 531 1135 681 191
Cuts at: 5560 826
Size: 1471
Fragments arranged by size:
1471 1135 1067 782 681 531 219 191
128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4309 5210 5472 820
Size: 197 926 2366 901 262 1553
Fragments arranged by size:
2366 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 3261 3644 513
Size: 552 2196 383 3074
Fragments arranged by size:
3074 2196 552 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3195 4232 4249 4402 4587 5032 325
Size: 1255 1615 1037 17 153 185 445 1498
Fragments arranged by size:
1615 1498 1255 1037 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4451 6163 96
Size: 274 80 33 51 1443 2474 1712 138
Fragments arranged by size:
2474 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 6165 6197 3365
Size: 32 2768 32 3373
Fragments arranged by size:
3373 2768 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3269 1316
Size: 1953 4252
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI