Restriction map for
pFN21AE2475
(Circular) (Six-base) MAPSORT of: pFN21AE2475.seq Check: 4011 from: 1 to: 5797
pFN21AE2475 5797 bp
With 182 enzymes: SgfI *
May 14, 2013 11:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2889 2936 3011 3030 3860 5244 5512
Size: 522 735 47 75 19 830 1384 268
Cuts at: 5512 1632
Size: 1917
Fragments arranged by size:
1917 1384 830 735 522 268 75 47
19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 5765
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaLI G'TGCA_C
Cuts at: 2436 3854 5010 2436
Size: 1418 1156 3223
Fragments arranged by size:
3223 1418 1156
ApoI r'AATT_y
Cuts at: 1493 2047 2591 2952 3106 3332 3484 5593 1493
Size: 554 544 361 154 226 152 2109 1697
Fragments arranged by size:
2109 1697 554 544 361 226 154 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
AvrII C'CTAG_G
Cuts at: 2737 2737
Size: 5797
BamHI G'GATC_C
Cuts at: 2080 3127 3494 2080
Size: 1047 367 4383
Fragments arranged by size:
4383 1047 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2792 3116 1391
Size: 1401 324 4072
Fragments arranged by size:
4072 1401 324
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 2175 1548
Size: 627 5170
BfrBI ATG'CAT
Cuts at: 2699 2699
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2440 3858 5014 1790
Size: 650 1418 1156 2573
Fragments arranged by size:
2573 1418 1156 650
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 3528 4486
Size: 416 53 83 399 24 917 1194 958
Cuts at: 4486 442
Size: 1753
Fragments arranged by size:
1753 1194 958 917 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4457 1239
Size: 3218 2579
Bpu10I CC'TnA_GC
Cuts at: 2619 2619
Size: 5797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2838 2968 3922 4787 5085 2838
Size: 130 954 865 298 3550
Fragments arranged by size:
3550 954 865 298 130
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 3040 5791 3040
Size: 2751 3046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2749 4230 4628 4902 5049 5460 2749
Size: 1481 398 274 147 411 3086
Fragments arranged by size:
3086 1481 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 2752 1186
Size: 1384 182 4231
Fragments arranged by size:
4231 1384 182
BseYI C'CCAG_C
Cuts at: 2791 5000 5496 2791
Size: 2209 496 3092
Fragments arranged by size:
3092 2209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2440 2854 3858 3943 5014 2440
Size: 414 1004 85 1071 3223
Fragments arranged by size:
3223 1071 1004 414 85
BsmI GAATG_Cn'
Cuts at: 2209 3020 3056 3308 3401 2209
Size: 811 36 252 93 4605
Fragments arranged by size:
4605 811 252 93 36
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2440 2792 2854 3116 3858 3943 5014
Size: 399 650 352 62 262 742 85 1071
Cuts at: 5014 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 650 399 352 262 85
62
BspEI T'CCGG_A
Cuts at: 2749 5460 2749
Size: 2711 3086
BspHI T'CATG_A
Cuts at: 2422 3686 5416 2422
Size: 1264 1730 2803
Fragments arranged by size:
2803 1730 1264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3152 844
Size: 2308 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 3025 4292 4466 65
Size: 1183 777 1000 1267 174 1396
Fragments arranged by size:
1396 1267 1183 1000 777 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2382 4441 4628 1700
Size: 110 572 2059 187 2869
Fragments arranged by size:
2869 2059 572 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2204 794
Size: 1410 4387
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 2344 1184
Size: 1160 4637
BstYI r'GATC_y
Cuts at: 1972 2080 3127 3494 3879 3896 5348 5434 5446
Size: 108 1047 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 108 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3313 4107 4127 2640
Size: 673 794 20 4310
Fragments arranged by size:
4310 794 673 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3045 3080 529
Size: 35 2481 35 3246
Fragments arranged by size:
3246 2481 35 35
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3244 4133 5762
Size: 54 1425 50 275 18 1414 889 1629
Cuts at: 5762 8
Size: 43
Fragments arranged by size:
1629 1425 1414 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2889 2936 3011 3030 3860 4457
Size: 393 522 735 47 75 19 830 597
Cuts at: 4457 5244 5512 1239
Size: 787 268 1524
Fragments arranged by size:
1524 830 787 735 597 522 393 268
75 47 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2222 2222
Size: 5797
EcoO109I rG'GnC_Cy
Cuts at: 2138 2138
Size: 5797
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 4944 1678
Size: 3266 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2014 2046 2243 2275 2303
Size: 32 135 32 442 32 197 32 28
Cuts at: 2303 2335 4438 4470 4512 4544 1373
Size: 32 2103 32 42 32 2626
Fragments arranged by size:
2626 2103 442 197 135 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3141 3387 669
Size: 988 281 894 309 246 3079
Fragments arranged by size:
3079 988 894 309 281 246
HindIII A'AGCT_T
Cuts at: 748 2930 748
Size: 2182 3615
HpaI GTT'AAC
Cuts at: 2832 3387 2832
Size: 555 5242
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 3755 4114 4273 518
Size: 648 1248 1341 359 159 2042
Fragments arranged by size:
2042 1341 1248 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2651 2837 3213 3890 5038 5283 1606
Size: 1045 186 376 677 1148 245 2120
Fragments arranged by size:
2120 1148 1045 677 376 245 186
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NsiI A_TGCA'T
Cuts at: 2701 2701
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2152 2850 3155 4700 1132
Size: 97 923 698 305 1545 2229
Fragments arranged by size:
2229 1545 923 698 305 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5797
PfoI T'CCnGG_A
Cuts at: 2083 2083
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2138 2138
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2651 2651
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SexAI A'CCwGG_T
Cuts at: 2158 2158
Size: 5797
SfcI C'TryA_G
Cuts at: 826 1045 3145 4280 4961 5152 826
Size: 219 2100 1135 681 191 1471
Fragments arranged by size:
2100 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2853 2947 3901 4802 5064 820
Size: 197 926 910 94 954 901 262 1553
Fragments arranged by size:
1553 954 926 910 901 262 197 94
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2209 2223 2674 2737 2796 3236 513
Size: 552 1144 14 451 63 59 440 3074
Fragments arranged by size:
3074 1144 552 451 440 63 59 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4043 5755 96
Size: 274 80 33 51 1443 2066 1712 138
Fragments arranged by size:
2066 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 2521 2553 5757 5789 2079
Size: 32 410 32 3204 32 2087
Fragments arranged by size:
3204 2087 410 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI