Restriction map for
pFN21AE2473
(Circular) (Six-base) MAPSORT of: pFN21AE2473.seq Check: 9357 from: 1 to: 7231
pFN21AE2473 7231 bp
With 182 enzymes: SgfI *
December 3, 2014 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3069 3069
Size: 7231
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5041 278
Size: 53 83 186 936 3505 2468
Fragments arranged by size:
3505 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2714 4574 1937
Size: 777 1860 4594
Fragments arranged by size:
4594 1860 777
Acc65I G'GTAC_C
Cuts at: 4552 4552
Size: 7231
AclI AA'CG_TT
Cuts at: 1563 5358 5731 1563
Size: 3795 373 3063
Fragments arranged by size:
3795 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3202 3778 4164 4199 4521 5294 6678
Size: 602 968 576 386 35 322 773 1384
Cuts at: 6678 6946 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 968 773 602 576 386 322
268 35
AfeI AGC'GCT
Cuts at: 3478 4272 3478
Size: 794 6437
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7034
AflIII A'CryG_T
Cuts at: 1203 3693 3828 4464 6130 7107 1203
Size: 2490 135 636 1666 977 1327
Fragments arranged by size:
2490 1666 1327 977 636 135
AgeI A'CCGG_T
Cuts at: 6062 6062
Size: 7231
AhdI GACnn_n'nnGTC
Cuts at: 5960 5960
Size: 7231
AleI CACnn'nnGTG
Cuts at: 1166 2096 2521 1166
Size: 930 425 5876
Fragments arranged by size:
5876 930 425
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3917 4265 1184
Size: 2733 348 4150
Fragments arranged by size:
4150 2733 348
AlwNI CAG_nnn'CTG
Cuts at: 2410 3511 4974 6546 2410
Size: 1101 1463 1572 3095
Fragments arranged by size:
3095 1572 1463 1101
ApaI G_GGCC'C
Cuts at: 2043 2308 2623 3750 3875 2043
Size: 265 315 1127 125 5399
Fragments arranged by size:
5399 1127 315 265 125
ApaLI G'TGCA_C
Cuts at: 3788 5288 6444 3788
Size: 1500 1156 4575
Fragments arranged by size:
4575 1500 1156
ApoI r'AATT_y
Cuts at: 1493 4540 4766 4918 7027 1493
Size: 3047 226 152 2109 1697
Fragments arranged by size:
3047 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5785 160
Size: 872 4753 1606
Fragments arranged by size:
4753 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7231
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 2769 3351 3524 4443 4556 1870
Size: 73 355 471 582 173 919 113 4545
Fragments arranged by size:
4545 919 582 471 355 173 113 73
BamHI G'GATC_C
Cuts at: 3006 4561 4928 3006
Size: 1555 367 5309
Fragments arranged by size:
5309 1555 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2357 2848 2893 3084 3093
Size: 325 217 627 570 491 45 191 9
Cuts at: 3093 3605 3647 4552 6007 618
Size: 512 42 905 1455 1842
Fragments arranged by size:
1842 1455 905 627 570 512 491 325
217 191 45 42 9
BanII G_rGCy'C
Cuts at: 1391 2043 2308 2623 2817 3072 3525 3531 3750
Size: 652 265 315 194 255 453 6 219
Cuts at: 3750 3875 4550 1391
Size: 125 675 4072
Fragments arranged by size:
4072 675 652 453 315 265 255 219
194 125 6
BbeI G_GCGC'C
Cuts at: 2361 3097 2361
Size: 736 6495
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2926 4509 928
Size: 971 1027 1583 3650
Fragments arranged by size:
3650 1583 1027 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 5422 5456 1903
Size: 34 2384 34 1067 34 3678
Fragments arranged by size:
3678 2384 1067 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4270 5123 6333 4270
Size: 853 1210 5168
Fragments arranged by size:
5168 1210 853
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7231
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2276 2280 2289
Size: 107 122 71 631 605 604 4 9
Cuts at: 2289 2303 4133 4301 5842 136
Size: 14 1830 168 1541 1525
Fragments arranged by size:
1830 1541 1525 631 605 604 168 122
107 71 14 9 4
BglII A'GATC_T
Cuts at: 7226 7226
Size: 7231
BlpI GC'TnA_GC
Cuts at: 3155 4184 4648 3155
Size: 1029 464 5738
Fragments arranged by size:
5738 1029 464
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2103 2294 2308 2623 2836 3134 3358
Size: 253 60 191 14 315 213 298 224
Cuts at: 3358 3750 3792 3875 4014 5292 6448 1790
Size: 392 42 83 139 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 392 315 298 253 224
213 191 139 83 60 42 14
BmgBI CAC'GTC
Cuts at: 1318 3027 3829 1318
Size: 1709 802 4720
Fragments arranged by size:
4720 1709 802
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2632 4962 5920
Size: 416 53 83 399 24 1215 2330 958
Cuts at: 5920 442
Size: 1753
Fragments arranged by size:
2330 1753 1215 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2799 2799
Size: 7231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2363 2613 2933 3759 5891 1239
Size: 1124 250 320 826 2132 2579
Fragments arranged by size:
2579 2132 1124 826 320 250
Bpu10I CC'TnA_GC
Cuts at: 4104 4104
Size: 7231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 4214 5356 6221 6519 2924
Size: 1290 1142 865 298 3636
Fragments arranged by size:
3636 1290 1142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2456 2593 2618 3999 5894 882
Size: 617 957 137 25 1381 1895 2219
Fragments arranged by size:
2219 1895 1381 957 617 137 25
BsaAI yAC'GTr
Cuts at: 493 1204 2224 5022 493
Size: 711 1020 2798 2702
Fragments arranged by size:
2798 2702 1020 711
BsaBI GATnn'nnATC
Cuts at: 7225 7225
Size: 7231
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2158 2358 3094 5038
Size: 53 83 186 936 625 200 736 1944
Cuts at: 5038 5420 6055 275
Size: 382 635 1451
Fragments arranged by size:
1944 1451 936 736 635 625 382 200
186 83 53
BsaWI w'CCGG_w
Cuts at: 3990 5664 6062 6336 6483 6894 3990
Size: 1674 398 274 147 411 4327
Fragments arranged by size:
4327 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3455 3485 3680 3710 1178
Size: 30 165 30 2052 30 195 30 4699
Fragments arranged by size:
4699 2052 195 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 3521 3728 1186
Size: 1264 1071 207 4689
Fragments arranged by size:
4689 1264 1071 207
BseYI C'CCAG_C
Cuts at: 4076 6434 6930 4076
Size: 2358 496 4377
Fragments arranged by size:
4377 2358 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3035 3047 1163
Size: 573 1299 12 5347
Fragments arranged by size:
5347 1299 573 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4066 4681 5442 6470 1540
Size: 275 186 2065 615 761 1028 2301
Fragments arranged by size:
2301 2065 1028 761 615 275 186
BsiHKAI G_wGCw'C
Cuts at: 2802 3072 3600 3792 3896 5292 5377 6448 2802
Size: 270 528 192 104 1396 85 1071 3585
Fragments arranged by size:
3585 1396 1071 528 270 192 104 85
BsmI GAATG_Cn'
Cuts at: 4742 4835 4742
Size: 93 7138
BsmBI CGTCTCn'nnnn_
Cuts at: 3033 3380 3033
Size: 347 6884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2103 2294 2308 2623 2802 2817
Size: 399 253 60 191 14 315 179 15
Cuts at: 2817 2836 3072 3134 3358 3525 3531 3600 3750
Size: 19 236 62 224 167 6 69 150
Cuts at: 3750 3792 3875 3896 4014 4550 5292 5377 6448
Size: 42 83 21 118 536 742 85 1071
Cuts at: 6448 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 536 399 315 253 236
224 191 179 167 150 118 85 83
69 62 60 42 21 19 15 14
6
BspEI T'CCGG_A
Cuts at: 6894 6894
Size: 7231
BspHI T'CATG_A
Cuts at: 2659 5120 6850 2659
Size: 2461 1730 3040
Fragments arranged by size:
3040 2461 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2542 2644 2997 3069 4586 844
Size: 1698 102 353 72 1517 3489
Fragments arranged by size:
3489 1698 1517 353 102 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 2389 4171 4188 5118 1394
Size: 429 344 222 1782 17 930 3507
Fragments arranged by size:
3507 1782 930 429 344 222 17
BsrDI GCAATG_nn'
Cuts at: 65 1248 2421 2648 4212 5726 5900 65
Size: 1183 1173 227 1564 1514 174 1396
Fragments arranged by size:
1564 1514 1396 1183 1173 227 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2201 2270 2354 3536 5875 6062 1700
Size: 110 391 69 84 1182 2339 187 2869
Fragments arranged by size:
2869 2339 1182 391 187 110 84 69
BsrGI T'GTAC_A
Cuts at: 96 3624 96
Size: 3528 3703
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7231
BssSI C'ACGA_G
Cuts at: 2096 3065 3357 4427 5291 6303 2096
Size: 969 292 1070 864 1012 3024
Fragments arranged by size:
3024 1070 1012 969 864 292
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2025 794
Size: 1231 6000
BstBI TT'CG_AA
Cuts at: 6035 6035
Size: 7231
BstEII G'GTnAC_C
Cuts at: 1184 3973 1184
Size: 2789 4442
BstXI CCAn_nnnn'nTGG
Cuts at: 2523 2957 4345 2523
Size: 434 1388 5409
Fragments arranged by size:
5409 1388 434
BstYI r'GATC_y
Cuts at: 1972 3006 4383 4561 4928 5313 5330 6782 6868
Size: 1034 1377 178 367 385 17 1452 86
Cuts at: 6868 6880 7226 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1377 1034 385 367 346 178
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 4521 513
Size: 552 939 39 2478 3223
Fragments arranged by size:
3223 2478 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4027 487
Size: 683 56 2801 3691
Fragments arranged by size:
3691 2801 683 56
BtsI GCAGTG_nn'
Cuts at: 2292 2309 3074 3369 4747 5541 5561 2292
Size: 17 765 295 1378 794 20 3962
Fragments arranged by size:
3962 1378 794 765 295 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7196
DraI TTT'AAA
Cuts at: 4535 4535
Size: 7231
DraIII CAC_nnn'GTG
Cuts at: 3303 3303
Size: 7231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6238 809
Size: 507 4922 1802
Fragments arranged by size:
4922 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2268 2281
Size: 54 1425 50 275 18 411 27 13
Cuts at: 2281 2352 3189 4063 4311 4678 5567 7196 8
Size: 71 837 874 248 367 889 1629 43
Fragments arranged by size:
1629 1425 889 874 837 411 367 275
248 71 54 50 43 27 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 4063 4678 1537
Size: 275 2251 615 4090
Fragments arranged by size:
4090 2251 615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2151 2658 3884 5161 1353
Size: 798 507 1226 1277 3423
Fragments arranged by size:
3423 1277 1226 798 507
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2732 5818 6192 6338 387
Size: 1064 138 1143 3086 374 146 1280
Fragments arranged by size:
3086 1280 1143 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3070 3070
Size: 7231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2363 2613 2933 3202 3759 3778
Size: 393 602 129 250 320 269 557 19
Cuts at: 3778 4164 4199 4521 5294 5891 6678 6946 1239
Size: 386 35 322 773 597 787 268 1524
Fragments arranged by size:
1524 787 773 602 597 557 393 386
322 320 269 268 250 129 35 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3655 3741 3655
Size: 86 7145
EcoO109I rG'GnC_Cy
Cuts at: 2039 2304 2305 2620 3730 3746 3747 2039
Size: 265 1 315 1110 16 1 5523
Fragments arranged by size:
5523 1110 315 265 16 1 1
EcoRI G'AATT_C
Cuts at: 4540 4918 4540
Size: 378 6853
FseI GG_CCGG'CC
Cuts at: 2205 2274 2205
Size: 69 7162
FspI TGC'GCA
Cuts at: 2993 3553 5737 2993
Size: 560 2184 4487
Fragments arranged by size:
4487 2184 560
HaeII r_GCGC'y
Cuts at: 1678 2261 2361 3097 3480 4274 6378 1678
Size: 583 100 736 383 794 2104 2531
Fragments arranged by size:
2531 2104 794 736 583 383 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 3109 3141 3426
Size: 32 135 32 602 32 903 32 285
Cuts at: 3426 3455 3458 3487 3917 3949 4250 4282 5872
Size: 29 3 29 430 32 301 32 1590
Cuts at: 5872 5904 5946 5978 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1590 903 602 430 301 285 135
42 32 32 32 32 32 32 32
32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4575 4821 669
Size: 988 281 2637 246 3079
Fragments arranged by size:
3079 2637 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7231
HpaI GTT'AAC
Cuts at: 4821 4821
Size: 7231
KasI G'GCGC_C
Cuts at: 2357 3093 2357
Size: 736 6495
KpnI G_GTAC'C
Cuts at: 4556 4556
Size: 7231
MfeI C'AATT_G
Cuts at: 3637 4830 3637
Size: 1193 6038
MluI A'CGCG_T
Cuts at: 7107 7107
Size: 7231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6345 6529 1305
Size: 5040 184 2007
Fragments arranged by size:
5040 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 4313 10
Size: 54 1425 343 411 2070 2928
Fragments arranged by size:
2928 2070 1425 411 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2096 2521 3683 3842 4343 5189 5548
Size: 648 930 425 1162 159 501 846 359
Cuts at: 5548 5707 518
Size: 159 2042
Fragments arranged by size:
2042 1162 930 846 648 501 425 359
159 159
MspA1I CmG'CkG
Cuts at: 1606 2174 2233 2483 2791 3622 4076 4474 4647
Size: 568 59 250 308 831 454 398 173
Cuts at: 4647 5324 6472 6717 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 568 454 398 308
250 245 173 59
NaeI GCC'GGC
Cuts at: 2203 2272 2356 3538 2203
Size: 69 84 1182 5896
Fragments arranged by size:
5896 1182 84 69
NarI GG'CG_CC
Cuts at: 2358 3094 2358
Size: 736 6495
NcoI C'CATG_G
Cuts at: 513 1065 2004 4521 513
Size: 552 939 2517 3223
Fragments arranged by size:
3223 2517 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7231
NgoMIV G'CCGG_C
Cuts at: 2201 2270 2354 3536 2201
Size: 69 84 1182 5896
Fragments arranged by size:
5896 1182 84 69
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7231
NotI GC'GGCC_GC
Cuts at: 4678 4678
Size: 7231
NspI r_CATG'y
Cuts at: 1132 1229 3226 3631 3697 4468 4589 6134 1132
Size: 97 1997 405 66 771 121 1545 2229
Fragments arranged by size:
2229 1997 1545 771 405 121 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7231
PciI A'CATG_T
Cuts at: 3693 4464 6130 3693
Size: 771 1666 4794
Fragments arranged by size:
4794 1666 771
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2677 3511 1108
Size: 1569 834 4828
Fragments arranged by size:
4828 1569 834
PfoI T'CCnGG_A
Cuts at: 3736 3736
Size: 7231
PmeI GTTT'AAAC
Cuts at: 4535 4535
Size: 7231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2820 2852 5300 5332 6813 6845 2820
Size: 32 2448 32 1481 32 3206
Fragments arranged by size:
3206 2448 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3730 3730
Size: 7231
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7231
PsiI TTA'TAA
Cuts at: 4801 4801
Size: 7231
PspOMI G'GGCC_C
Cuts at: 2039 2304 2619 3746 3871 2039
Size: 265 315 1127 125 5399
Fragments arranged by size:
5399 1127 315 265 125
PspXI vC'TCGA_Gb
Cuts at: 2769 2769
Size: 7231
PstI C_TGCA'G
Cuts at: 830 2156 2232 2790 3825 4114 4479 4583 830
Size: 1326 76 558 1035 289 365 104 3478
Fragments arranged by size:
3478 1326 1035 558 365 289 104 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7231
PvuII CAG'CTG
Cuts at: 2233 2483 2791 3622 4076 4474 2233
Size: 250 308 831 454 398 4990
Fragments arranged by size:
4990 831 454 398 308 250
SacI G_AGCT'C
Cuts at: 3072 3072
Size: 7231
SalI G'TCGA_C
Cuts at: 1936 4573 1936
Size: 2637 4594
SapI GCTCTTCn'nnn_
Cuts at: 2151 3884 2151
Size: 1733 5498
SbfI CC_TGCA'GG
Cuts at: 4583 4583
Size: 7231
ScaI AGT'ACT
Cuts at: 5479 7191 5479
Size: 1712 5519
SexAI A'CCwGG_T
Cuts at: 3162 3510 3162
Size: 348 6883
SfcI C'TryA_G
Cuts at: 826 1045 2152 2228 2786 2860 3578 3655 3821
Size: 219 1107 76 558 74 718 77 166
Cuts at: 3821 4110 4475 4579 5714 6395 6586 826
Size: 289 365 104 1135 681 191 1471
Fragments arranged by size:
1471 1135 1107 718 681 558 365 289
219 191 166 104 77 76 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 2280 2280
Size: 7231
SfoI GGC'GCC
Cuts at: 2359 3095 2359
Size: 736 6495
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7231
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7231
SmaI CCC'GGG
Cuts at: 1872 2300 3353 4558 1872
Size: 428 1053 1205 4545
Fragments arranged by size:
4545 1205 1053 428
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2769 2939 4229 5335 6236 6498
Size: 197 926 826 170 1290 1106 901 262
Cuts at: 6498 820
Size: 1553
Fragments arranged by size:
1553 1290 1106 926 901 826 262 197
170
SnaBI TAC'GTA
Cuts at: 493 5022 493
Size: 4529 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7231
SphI G_CATG'C
Cuts at: 1132 4589 1132
Size: 3457 3774
SspI AAT'ATT
Cuts at: 5 52 5155 5
Size: 47 5103 2081
Fragments arranged by size:
5103 2081 47
StuI AGG'CCT
Cuts at: 1517 3983 1517
Size: 2466 4765
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2897 4521 4670 513
Size: 552 939 129 764 1624 149 3074
Fragments arranged by size:
3074 1624 939 764 552 149 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2560 2685 5258 5275 5428 5613 6058
Size: 1255 980 125 2573 17 153 185 445
Cuts at: 6058 325
Size: 1498
Fragments arranged by size:
2573 1498 1255 980 445 185 153 125
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2969 3624 5477
Size: 274 80 33 51 1443 992 655 1853
Cuts at: 5477 7189 96
Size: 1712 138
Fragments arranged by size:
1853 1712 1443 992 655 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7191 7223 7191
Size: 32 7199
Tth111I GACn'n_nGTC
Cuts at: 1316 2631 4022 1316
Size: 1315 1391 4525
Fragments arranged by size:
4525 1391 1315
XbaI T'CTAG_A
Cuts at: 4567 4567
Size: 7231
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2523 4342 2011
Size: 512 1819 4900
Fragments arranged by size:
4900 1819 512
XhoI C'TCGA_G
Cuts at: 1943 2769 1943
Size: 826 6405
XmaI C'CCGG_G
Cuts at: 1870 2298 3351 4556 1870
Size: 428 1053 1205 4545
Fragments arranged by size:
4545 1205 1053 428
XmnI GAAnn'nnTTC
Cuts at: 5360 5360
Size: 7231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5039 276
Size: 53 83 186 936 3505 2468
Fragments arranged by size:
3505 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoRV FalI FspAI NruI NsiI PacI PmlI
PsrI RsrII SacII SanDI SrfI SwaI