Restriction map for
pFN21AE2471
(Circular) (Six-base) MAPSORT of: pFN21AE2471.seq Check: 2241 from: 1 to: 6496
pFN21AE2471 6496 bp
With 182 enzymes: SgfI *
May 21, 2013 17:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3738 4306 278
Size: 53 83 186 936 2202 568 2468
Fragments arranged by size:
2468 2202 936 568 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3839 1937
Size: 1902 4594
Acc65I G'GTAC_C
Cuts at: 3817 3817
Size: 6496
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2444 2793 2874 3002 3401 3746 4559
Size: 488 324 349 81 128 399 345 813
Cuts at: 4559 5943 6211 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 813 488 399 349 345 324
268 128 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 5395 6372 1203
Size: 4192 977 1327
Fragments arranged by size:
4192 1327 977
AgeI A'CCGG_T
Cuts at: 2949 5327 2949
Size: 2378 4118
AhdI GACnn_n'nnGTC
Cuts at: 3208 5225 3208
Size: 2017 4479
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2433 2465 2433
Size: 32 6464
AlwNI CAG_nnn'CTG
Cuts at: 3604 3776 4239 5811 3604
Size: 172 463 1572 4289
Fragments arranged by size:
4289 1572 463 172
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 2603 3805 4031 4183 6292 1493
Size: 1110 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 1110 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5050 160
Size: 872 4018 1606
Fragments arranged by size:
4018 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 2934 3821 1870
Size: 73 991 887 4545
Fragments arranged by size:
4545 991 887 73
AvrII C'CTAG_G
Cuts at: 3439 3439
Size: 6496
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2739 2772 2739
Size: 33 6463
BamHI G'GATC_C
Cuts at: 2135 3826 4193 2135
Size: 1691 367 4438
Fragments arranged by size:
4438 1691 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 3817 5272 618
Size: 325 217 627 768 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 768 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3077 3815 1391
Size: 1686 738 4072
Fragments arranged by size:
4072 1686 738
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5525
BbvCI CC'TCA_GC
Cuts at: 3653 3653
Size: 6496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2042 2076 3577 3611 4687 4721 1903
Size: 34 105 34 1501 34 1076 34 3678
Fragments arranged by size:
3678 1501 1076 105 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5107 136
Size: 107 122 71 631 605 3435 1525
Fragments arranged by size:
3435 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6491 6491
Size: 6496
BlpI GC'TnA_GC
Cuts at: 3272 3518 3913 3272
Size: 246 395 5855
Fragments arranged by size:
5855 395 246
Bme1580I G_kGCm'C
Cuts at: 1790 4557 5713 1790
Size: 2767 1156 2573
Fragments arranged by size:
2767 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4227 5185 442
Size: 416 53 83 399 24 2810 958 1753
Fragments arranged by size:
2810 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2134 3067 3139 3396 3485 3753 5156
Size: 857 38 933 72 257 89 268 1403
Cuts at: 5156 1239
Size: 2579
Fragments arranged by size:
2579 1403 933 857 268 257 89 72
38
Bpu10I CC'TnA_GC
Cuts at: 3653 3653
Size: 6496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3091 4621 5486 5784 3091
Size: 1530 865 298 3803
Fragments arranged by size:
3803 1530 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3047 3348 5159 882
Size: 617 1548 301 1811 2219
Fragments arranged by size:
2219 1811 1548 617 301
BsaAI yAC'GTr
Cuts at: 493 1204 2899 4287 493
Size: 711 1695 1388 2702
Fragments arranged by size:
2702 1695 1388 711
BsaBI GATnn'nnATC
Cuts at: 6490 6490
Size: 6496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3735 4303 4685 5320
Size: 53 83 186 936 2202 568 382 635
Cuts at: 5320 275
Size: 1451
Fragments arranged by size:
2202 1451 936 635 568 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2949 4929 5327 5601 5748 6159 2949
Size: 1980 398 274 147 411 3286
Fragments arranged by size:
3286 1980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2433 2463 3458 3488 3641
Size: 30 165 30 1030 30 995 30 153
Cuts at: 3641 3671 1178
Size: 30 4003
Fragments arranged by size:
4003 1030 995 165 153 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2906 3062 3065 1186
Size: 1720 156 3 4617
Fragments arranged by size:
4617 1720 156 3
BseYI C'CCAG_C
Cuts at: 3112 3299 5699 6195 3112
Size: 187 2400 496 3413
Fragments arranged by size:
3413 2400 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3281 3590 1163
Size: 573 1545 309 4069
Fragments arranged by size:
4069 1545 573 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3030 3641 3946 4707 5735 1540
Size: 275 186 1029 611 305 761 1028 2301
Fragments arranged by size:
2301 1029 1028 761 611 305 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2420 2898 2919 3077 4557 4642 5713 2304
Size: 116 478 21 158 1480 85 1071 3087
Fragments arranged by size:
3087 1480 1071 478 158 116 85 21
BsmI GAATG_Cn'
Cuts at: 2866 4007 4100 2866
Size: 1141 93 5262
Fragments arranged by size:
5262 1141 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3575 3575
Size: 6496
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2420 2898 2919 3077 3815 4557
Size: 399 514 116 478 21 158 738 742
Cuts at: 4557 4642 5713 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 738 514 478 399 158
116 85 21
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 4385 6115 4385
Size: 1730 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3612 3851 844
Size: 2768 239 3489
Fragments arranged by size:
3489 2768 239
BsrBI CCG'CTC
Cuts at: 1394 1823 4383 1394
Size: 429 2560 3507
Fragments arranged by size:
3507 2560 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2646 2746 4991 5165 65
Size: 1183 1398 100 2245 174 1396
Fragments arranged by size:
2245 1398 1396 1183 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 2949 5140 5327 1700
Size: 110 1139 2191 187 2869
Fragments arranged by size:
2869 2191 1139 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 4556 5568 4556
Size: 1012 5484
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3604 794
Size: 2810 3686
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2135 2470 2688 3826 4193 4578 4595 6047
Size: 163 335 218 1138 367 385 17 1452
Cuts at: 6047 6133 6145 6491 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 335 218
163 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2476 2544 513
Size: 552 939 472 68 4465
Fragments arranged by size:
4465 939 552 472 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5757
Fragments arranged by size:
5757 683 56
BtsI GCAGTG_nn'
Cuts at: 3269 4012 4806 4826 3269
Size: 743 794 20 4939
Fragments arranged by size:
4939 794 743 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6461
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DraIII CAC_nnn'GTG
Cuts at: 3064 3064
Size: 6496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5503 809
Size: 507 4187 1802
Fragments arranged by size:
4187 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 2375 3027
Size: 54 1425 50 275 18 310 235 652
Cuts at: 3027 3638 3943 4832 6461 8
Size: 611 305 889 1629 43
Fragments arranged by size:
1629 1425 889 652 611 310 305 275
235 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3027 3638 3943 1537
Size: 275 1215 611 305 4090
Fragments arranged by size:
4090 1215 611 305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2268 2978 3377 4426 1353
Size: 915 710 399 1049 3423
Fragments arranged by size:
3423 1049 915 710 399
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2441 3366 3635 5083 5457 5603
Size: 1064 138 852 925 269 1448 374 146
Cuts at: 5603 387
Size: 1280
Fragments arranged by size:
1448 1280 1064 925 852 374 269 146
138
EcoICRI GAG'CTC
Cuts at: 3075 3075
Size: 6496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2120 2134 2444 2793 2874 3002
Size: 393 464 24 14 310 349 81 128
Cuts at: 3002 3067 3139 3396 3401 3485 3746 3753 4559
Size: 65 72 257 5 84 261 7 806
Cuts at: 4559 5156 5943 6211 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 806 787 597 464 393 349 310
268 261 257 128 84 81 72 65
24 14 7 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3676 3676
Size: 6496
EcoO109I rG'GnC_Cy
Cuts at: 3601 3601
Size: 6496
EcoRI G'AATT_C
Cuts at: 3805 4183 3805
Size: 378 6118
EcoRV GAT'ATC
Cuts at: 3508 3508
Size: 6496
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2044 2909 5643 1678
Size: 366 865 2734 2531
Fragments arranged by size:
2734 2531 865 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2455 2487 2817
Size: 32 135 32 581 32 270 32 330
Cuts at: 2817 2849 2962 2994 3059 3091 3194 3226 3458
Size: 32 113 32 65 32 103 32 232
Cuts at: 3458 3490 5137 5169 5211 5243 1373
Size: 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 581 330 270 232 135 113
103 65 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 3840 4086 669
Size: 988 281 1084 818 246 3079
Fragments arranged by size:
3079 1084 988 818 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KpnI G_GTAC'C
Cuts at: 3821 3821
Size: 6496
MfeI C'AATT_G
Cuts at: 2508 4095 2508
Size: 1587 4909
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2086 3192 3672 3690 5610 5794 1305
Size: 781 1106 480 18 1920 184 2007
Fragments arranged by size:
2007 1920 1106 781 480 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4674
Fragments arranged by size:
4674 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 4454 4813 4972 518
Size: 648 2310 978 359 159 2042
Fragments arranged by size:
2310 2042 978 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2753 2838 2924 3379 3912 4589 5737 5982
Size: 1147 85 86 455 533 677 1148 245
Cuts at: 5982 1606
Size: 2120
Fragments arranged by size:
2120 1148 1147 677 533 455 245 86
85
NcoI C'CATG_G
Cuts at: 513 1065 2004 2476 2544 513
Size: 552 939 472 68 4465
Fragments arranged by size:
4465 939 552 472 68
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6496
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 2845 3854 5399 1132
Size: 97 1616 1009 1545 2229
Fragments arranged by size:
2229 1616 1545 1009 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3064 1108
Size: 1956 4540
PfoI T'CCnGG_A
Cuts at: 2436 2515 3333 2436
Size: 79 818 5599
Fragments arranged by size:
5599 818 79
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PmlI CAC'GTG
Cuts at: 2899 2899
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2433 2465 4565 4597 6078 6110 2433
Size: 32 2100 32 1481 32 2819
Fragments arranged by size:
2819 2100 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3601 3601
Size: 6496
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 4066 4066
Size: 6496
PstI C_TGCA'G
Cuts at: 830 2870 3282 3600 3848 830
Size: 2040 412 318 248 3478
Fragments arranged by size:
3478 2040 412 318 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 2753 2838 2924 3379 2753
Size: 85 86 455 5870
Fragments arranged by size:
5870 455 86 85
SacI G_AGCT'C
Cuts at: 3077 3077
Size: 6496
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SapI GCTCTTCn'nnn_
Cuts at: 3377 3377
Size: 6496
SbfI CC_TGCA'GG
Cuts at: 3282 3600 3848 3282
Size: 318 248 5930
Fragments arranged by size:
5930 318 248
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SfcI C'TryA_G
Cuts at: 826 1045 2866 3010 3278 3307 3596 3844 4979
Size: 219 1821 144 268 29 289 248 1135
Cuts at: 4979 5660 5851 826
Size: 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 289 268 248 219
191 144 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 3823 1872
Size: 1951 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3070 4600 5501 5763 820
Size: 197 926 1127 1530 901 262 1553
Fragments arranged by size:
1553 1530 1127 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 3854 1132
Size: 2722 3774
SspI AAT'ATT
Cuts at: 5 52 4420 5
Size: 47 4368 2081
Fragments arranged by size:
4368 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6496
StyI C'CwwG_G
Cuts at: 513 1065 2004 2476 2544 3423 3439 3935 513
Size: 552 939 472 68 879 16 496 3074
Fragments arranged by size:
3074 939 879 552 496 472 68 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3755 4523 4540 4693 4878 5323 325
Size: 1255 2175 768 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 768 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 2785 4742
Size: 274 80 33 51 1443 690 118 1957
Cuts at: 4742 6454 96
Size: 1712 138
Fragments arranged by size:
1957 1712 1443 690 274 138 118 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 6456 6488 2103
Size: 32 4321 32 2111
Fragments arranged by size:
4321 2111 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 3821 1870
Size: 1951 4545
XmnI GAAnn'nnTTC
Cuts at: 3703 4625 3703
Size: 922 5574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3736 4304 276
Size: 53 83 186 936 2202 568 2468
Fragments arranged by size:
2468 2202 936 568 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BfrBI BsiWI BstXI
BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI