Restriction map for
pFN21AE2470
(Circular) (Six-base) MAPSORT of: pFN21AE2470.seq Check: 7993 from: 1 to: 6127
pFN21AE2470 6127 bp
With 182 enzymes: SgfI *
May 21, 2013 17:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 3470 1937
Size: 955 578 4594
Fragments arranged by size:
4594 955 578
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3253 4190 5574 5842 1632
Size: 1621 937 1384 268 1917
Fragments arranged by size:
1917 1621 1384 937 268
AfeI AGC'GCT
Cuts at: 2043 2844 2043
Size: 801 5326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 3135 5026 6003 1203
Size: 1932 1891 977 1327
Fragments arranged by size:
1932 1891 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 2696 3870 5442 2696
Size: 1174 1572 3381
Fragments arranged by size:
3381 1572 1174
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2081 2149 2167 2854 3178 3359
Size: 73 125 13 68 18 687 324 181
Cuts at: 3359 3379 3392 3452 1870
Size: 20 13 60 4545
Fragments arranged by size:
4545 687 324 181 125 73 68 60
20 18 13 13
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2567 2756 3448 4903
Size: 325 217 627 285 495 189 692 1455
Cuts at: 4903 618
Size: 1842
Fragments arranged by size:
1842 1455 692 627 495 325 285 217
189
BanII G_rGCy'C
Cuts at: 1391 2063 3185 3318 3446 1391
Size: 672 1122 133 128 4072
Fragments arranged by size:
4072 1122 672 133 128
BbeI G_GCGC'C
Cuts at: 2076 2571 2760 2076
Size: 495 189 5443
Fragments arranged by size:
5443 495 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3216 928
Size: 971 1317 3839
Fragments arranged by size:
3839 1317 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4318 4352 1903
Size: 34 2381 34 3678
Fragments arranged by size:
3678 2381 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2505 2726 2822
Size: 107 122 71 631 605 833 221 96
Cuts at: 2822 3024 4738 136
Size: 202 1714 1525
Fragments arranged by size:
1714 1525 833 631 605 221 202 122
107 96 71
BglII A'GATC_T
Cuts at: 2268 6122 2268
Size: 3854 2273
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2915 4188 5344 1790
Size: 388 737 1273 1156 2573
Fragments arranged by size:
2573 1273 1156 737 388
BmgBI CAC'GTC
Cuts at: 1318 2916 1318
Size: 1598 4529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3337 3394 3407 3337
Size: 57 13 6057
Fragments arranged by size:
6057 57 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 4787 1239
Size: 1171 2377 2579
Fragments arranged by size:
2579 2377 1171
Bpu10I CC'TnA_GC
Cuts at: 3020 3020
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 4252 5117 5415 2339
Size: 1913 865 298 3051
Fragments arranged by size:
3051 1913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2568 2757 3934
Size: 53 83 186 936 540 495 189 1177
Cuts at: 3934 4316 4951 275
Size: 382 635 1451
Fragments arranged by size:
1451 1177 936 635 540 495 382 189
186 83 53
BsaWI w'CCGG_w
Cuts at: 4560 4958 5232 5379 5790 4560
Size: 398 274 147 411 4897
Fragments arranged by size:
4897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2414 2438 2444 2460
Size: 30 165 30 1005 6 24 6 16
Cuts at: 2460 2490 3125 3155 1178
Size: 30 635 30 4150
Fragments arranged by size:
4150 1005 635 165 30 30 30 30
24 16 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 2422 2437 2556 2809 2996 2999 3133
Size: 903 333 15 119 253 187 3 134
Cuts at: 3133 3151 3154 3163 3166 3175 1186
Size: 18 3 9 3 9 4138
Fragments arranged by size:
4138 903 333 253 187 134 119 18
15 9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 2086 2281 2798 2957 3014 3233 5330 5826 2086
Size: 195 517 159 57 219 2097 496 2387
Fragments arranged by size:
2387 2097 517 496 219 195 159 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 3337 1163
Size: 573 294 1307 3953
Fragments arranged by size:
3953 1307 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2439 3028 3061 3292 3577 4338
Size: 275 186 438 589 33 231 285 761
Cuts at: 4338 5366 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 589 438 285 275 231
186 33
BsiHKAI G_wGCw'C
Cuts at: 2063 4188 4273 5344 2063
Size: 2125 85 1071 2846
Fragments arranged by size:
2846 2125 1071 85
BsmI GAATG_Cn'
Cuts at: 3638 3731 3638
Size: 93 6034
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2178 2915 3185 3318 3446 4188
Size: 399 273 115 737 270 133 128 742
Cuts at: 4188 4273 5344 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 737 399 273 270 133
128 115 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3225 3482 844
Size: 2381 257 3489
Fragments arranged by size:
3489 2381 257
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 2344 3024 3063 4014 1394
Size: 429 401 120 680 39 951 3507
Fragments arranged by size:
3507 951 680 429 401 120 39
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2075 2388 2581 3056 4771 4958
Size: 110 241 24 313 193 475 1715 187
Cuts at: 4958 1700
Size: 2869
Fragments arranged by size:
2869 1715 475 313 241 193 187 110
24
BsrGI T'GTAC_A
Cuts at: 96 2781 96
Size: 2685 3442
BssHII G'CGCG_C
Cuts at: 1925 3097 3123 1925
Size: 1172 26 4929
Fragments arranged by size:
4929 1172 26
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2696 794
Size: 1902 4225
BstBI TT'CG_AA
Cuts at: 3207 4931 3207
Size: 1724 4403
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2281
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 2268 2319 3457 3824 4209 4226 5678 5764
Size: 296 51 1138 367 385 17 1452 86
Cuts at: 5764 5776 6122 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 296 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2500 2928 3079 513
Size: 552 939 123 373 428 151 3561
Fragments arranged by size:
3561 939 552 428 373 151 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 3643 4437 4457 2491
Size: 1152 794 20 4161
Fragments arranged by size:
4161 1152 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2461 2496 529
Size: 35 1897 35 4160
Fragments arranged by size:
4160 1897 35 35
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2436 2497
Size: 54 1425 50 275 18 219 387 61
Cuts at: 2497 3025 3058 3574 4463 6092 8
Size: 528 33 516 889 1629 43
Fragments arranged by size:
1629 1425 889 528 516 387 275 219
61 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2436 3025 3058 3574 1537
Size: 275 624 589 33 516 4090
Fragments arranged by size:
4090 624 589 516 275 33
EarI CTCTTCn'nnn_
Cuts at: 1353 3172 4057 1353
Size: 1819 885 3423
Fragments arranged by size:
3423 1819 885
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 2803 2885 4714 5088 5234
Size: 1064 138 765 449 82 1829 374 146
Cuts at: 5234 387
Size: 1280
Fragments arranged by size:
1829 1280 1064 765 449 374 146 138
82
EcoICRI GAG'CTC
Cuts at: 2061 2061
Size: 6127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 3253 4190 4787 5574 5842 1239
Size: 393 778 843 937 597 787 268 1524
Fragments arranged by size:
1524 937 843 787 778 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 6127
EcoO109I rG'GnC_Cy
Cuts at: 3295 3295
Size: 6127
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2045 2076 2571 2760 2805 2846 3039 3287
Size: 367 31 495 189 45 41 193 248
Cuts at: 3287 5274 1678
Size: 1987 2531
Fragments arranged by size:
2531 1987 495 367 248 193 189 45
41 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 2759 2791 3239
Size: 32 135 32 888 32 267 32 448
Cuts at: 3239 3271 4768 4800 4842 4874 1373
Size: 32 1497 32 42 32 2626
Fragments arranged by size:
2626 1497 888 448 267 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KasI G'GCGC_C
Cuts at: 2072 2567 2756 2072
Size: 495 189 5443
Fragments arranged by size:
5443 495 189
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 3135 6003 3135
Size: 2868 3259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3219 3432 5241 5425 1305
Size: 1914 213 1809 184 2007
Fragments arranged by size:
2007 1914 1809 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4085 4444 4603 518
Size: 648 2919 359 159 2042
Fragments arranged by size:
2919 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2693 3081 3543 4220 5368 5613 1606
Size: 1087 388 462 677 1148 245 2120
Fragments arranged by size:
2120 1148 1087 677 462 388 245
NaeI GCC'GGC
Cuts at: 2053 2077 3058 2053
Size: 24 981 5122
Fragments arranged by size:
5122 981 24
NarI GG'CG_CC
Cuts at: 2073 2568 2757 2073
Size: 495 189 5443
Fragments arranged by size:
5443 495 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 2500 513
Size: 552 939 496 4140
Fragments arranged by size:
4140 939 552 496
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NgoMIV G'CCGG_C
Cuts at: 2051 2075 3056 2051
Size: 24 981 5122
Fragments arranged by size:
5122 981 24
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3025 3574 3025
Size: 549 5578
NspI r_CATG'y
Cuts at: 1132 1229 2257 3256 3485 5030 1132
Size: 97 1028 999 229 1545 2229
Fragments arranged by size:
2229 1545 1028 999 229 97
PasI CC'CwG_GG
Cuts at: 1348 2752 2968 3259 1348
Size: 1404 216 291 4216
Fragments arranged by size:
4216 1404 291 216
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PfoI T'CCnGG_A
Cuts at: 3342 3342
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 4196 4228 5709 5741 3221
Size: 32 943 32 1481 32 3607
Fragments arranged by size:
3607 1481 943 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3295 3295
Size: 6127
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspXI vC'TCGA_Gb
Cuts at: 2081 3392 2081
Size: 1311 4816
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
SacI G_AGCT'C
Cuts at: 2063 2063
Size: 6127
SacII CC_GC'GG
Cuts at: 3082 3082
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SexAI A'CCwGG_T
Cuts at: 3411 3411
Size: 6127
SfcI C'TryA_G
Cuts at: 826 1045 3475 4610 5291 5482 826
Size: 219 2430 1135 681 191 1471
Fragments arranged by size:
2430 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2074 2569 2758 2074
Size: 495 189 5443
Fragments arranged by size:
5443 495 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 2856 3454 1872
Size: 984 598 4545
Fragments arranged by size:
4545 984 598
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 2354 3392 4231 5132 5394
Size: 197 926 138 273 1038 839 901 262
Cuts at: 5394 820
Size: 1553
Fragments arranged by size:
1553 1038 926 901 839 273 262 197
138
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2004 2500 3566 513
Size: 552 939 496 1066 3074
Fragments arranged by size:
3074 1066 939 552 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4154 4171 4324 4509 4954 325
Size: 1255 2574 17 153 185 445 1498
Fragments arranged by size:
2574 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 4373 6085
Size: 274 80 33 51 1443 804 1592 1712
Cuts at: 6085 96
Size: 138
Fragments arranged by size:
1712 1592 1443 804 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 6087 6119 3125
Size: 32 2930 32 3133
Fragments arranged by size:
3133 2930 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2474 1316
Size: 1158 4969
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 2965 2659
Size: 306 5821
XhoI C'TCGA_G
Cuts at: 1943 2081 3392 1943
Size: 138 1311 4678
Fragments arranged by size:
4678 1311 138
XmaI C'CCGG_G
Cuts at: 1870 2854 3452 1870
Size: 984 598 4545
Fragments arranged by size:
4545 984 598
XmnI GAAnn'nnTTC
Cuts at: 2198 4256 2198
Size: 2058 4069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PsrI PvuII
RsrII SanDI SapI SfiI SrfI SwaI