Restriction map for
pFN21AE2458
(Circular) (Six-base) MAPSORT of: pFN21AE2458.seq Check: 8069 from: 1 to: 5533
pFN21AE2458 5533 bp
With 182 enzymes: SgfI *
February 8, 2013 19:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2876 1937
Size: 939 4594
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 2610 3596 4980 5248 1632
Size: 896 82 986 1384 268 1917
Fragments arranged by size:
1917 1384 986 896 268 82
AfeI AGC'GCT
Cuts at: 2072 2072
Size: 5533
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 2133 4432 5409 1203
Size: 930 2299 977 1327
Fragments arranged by size:
2299 1327 977 930
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 2387 3276 4848 2387
Size: 889 1572 3072
Fragments arranged by size:
3072 1572 889
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2176 2784 2842 3068 3220 5329 1493
Size: 683 608 58 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 608 226 152 58
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2858 1870
Size: 73 73 842 4545
Fragments arranged by size:
4545 842 73 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2468 2854 4309 618
Size: 325 217 627 681 386 1455 1842
Fragments arranged by size:
1842 1455 681 627 386 325 217
BanII G_rGCy'C
Cuts at: 1391 2394 2653 2852 1391
Size: 1003 259 199 4072
Fragments arranged by size:
4072 1003 259 199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2388 2950 2388
Size: 562 4971
Bme1580I G_kGCm'C
Cuts at: 1790 3594 4750 1790
Size: 1804 1156 2573
Fragments arranged by size:
2573 1804 1156
BmgBI CAC'GTC
Cuts at: 1318 2767 1318
Size: 1449 4084
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 2825 3264
Size: 416 53 83 399 24 739 669 439
Cuts at: 3264 4222 442
Size: 958 1753
Fragments arranged by size:
1753 958 739 669 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4193 1239
Size: 2954 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3658 4523 4821 3658
Size: 865 298 4370
Fragments arranged by size:
4370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2403 4196 882
Size: 617 904 1793 2219
Fragments arranged by size:
2219 1793 904 617
BsaAI yAC'GTr
Cuts at: 493 1204 3324 493
Size: 711 2120 2702
Fragments arranged by size:
2702 2120 711
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 3340 3722 4357
Size: 53 83 186 936 510 1297 382 635
Cuts at: 4357 275
Size: 1451
Fragments arranged by size:
1451 1297 936 635 510 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5308
Fragments arranged by size:
5308 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5533
BseYI C'CCAG_C
Cuts at: 2708 4736 5232 2708
Size: 2028 496 3009
Fragments arranged by size:
3009 2028 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4960
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 3594 3679 4750 2394
Size: 1200 85 1071 3177
Fragments arranged by size:
3177 1200 1071 85
BsmI GAATG_Cn'
Cuts at: 2235 3044 3137 2235
Size: 809 93 4631
Fragments arranged by size:
4631 809 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2653 2852 3594 3679 4750 1391
Size: 399 604 259 199 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 259 199 85
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 2166 3422 5152 2166
Size: 1256 1730 2547
Fragments arranged by size:
2547 1730 1256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 2888 844
Size: 1206 838 3489
Fragments arranged by size:
3489 1206 838
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4028 4202 65
Size: 1183 2780 174 1396
Fragments arranged by size:
2780 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2512 2863 3230 3615 3632 5084 5170 5182
Size: 540 351 367 385 17 1452 86 12
Cuts at: 5182 5528 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 540 385 367 351 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4042
Fragments arranged by size:
4042 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4794
Fragments arranged by size:
4794 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 2758 3049 3843 3863 2374
Size: 384 291 794 20 4044
Fragments arranged by size:
4044 794 384 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2569 2837 2569
Size: 268 5265
DraIII CAC_nnn'GTG
Cuts at: 2188 2188
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2980 3869 5498
Size: 54 1425 50 275 18 1150 889 1629
Cuts at: 5498 8
Size: 43
Fragments arranged by size:
1629 1425 1150 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2097 2547 3463 1353
Size: 744 450 916 3423
Fragments arranged by size:
3423 916 744 450
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2392
Size: 5533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2610 3596 4193 4980 5248 1239
Size: 393 896 82 986 597 787 268 1524
Fragments arranged by size:
1524 986 896 787 597 393 268 82
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FspI TGC'GCA
Cuts at: 4039 4039
Size: 5533
HaeII r_GCGC'y
Cuts at: 1678 2038 2074 4680 1678
Size: 360 36 2606 2531
Fragments arranged by size:
2606 2531 360 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2524 2556 4174 4206 4248
Size: 32 135 32 952 32 1618 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 1618 952 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 4647 4831 1305
Size: 1185 2157 184 2007
Fragments arranged by size:
2157 2007 1185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3711
Fragments arranged by size:
3711 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2138 2165 2240 2474 3491 3850 4009
Size: 648 972 27 75 234 1017 359 159
Cuts at: 4009 518
Size: 2042
Fragments arranged by size:
2042 1017 972 648 359 234 159 75
27
MspA1I CmG'CkG
Cuts at: 1606 2048 2384 2718 2949 3626 4774 5019 1606
Size: 442 336 334 231 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 442 336 334 245 231
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4042
Fragments arranged by size:
4042 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2137 2891 4436 1132
Size: 97 908 754 1545 2229
Fragments arranged by size:
2229 1545 908 754 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 2133 4432 2133
Size: 2299 3234
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PstI C_TGCA'G
Cuts at: 830 2885 830
Size: 2055 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
PvuII CAG'CTG
Cuts at: 2384 2384
Size: 5533
SacI G_AGCT'C
Cuts at: 2394 2394
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SapI GCTCTTCn'nnn_
Cuts at: 2547 2547
Size: 5533
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2881 4016 4697 4888 826
Size: 219 1836 1135 681 191 1471
Fragments arranged by size:
1836 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2018 2860 1872
Size: 146 842 4545
Fragments arranged by size:
4545 842 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 4538 4800 820
Size: 197 926 1694 901 262 1553
Fragments arranged by size:
1694 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2972 513
Size: 552 939 968 3074
Fragments arranged by size:
3074 968 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3560 3577 3730 3915 4360 325
Size: 1255 1980 17 153 185 445 1498
Fragments arranged by size:
1980 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 1316
Size: 846 4687
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2016 2858 1870
Size: 146 842 4545
Fragments arranged by size:
4545 842 146
XmnI GAAnn'nnTTC
Cuts at: 2283 3662 2283
Size: 1379 4154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI