Restriction map for
pFN21AE2452
(Circular) (Six-base) MAPSORT of: pFN21AE2452.seq Check: 933 from: 1 to: 6292
pFN21AE2452 6292 bp
With 182 enzymes: SgfI *
February 5, 2013 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2066 3336 3450 2066
Size: 1270 114 4908
Fragments arranged by size:
4908 1270 114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2565 2769 4102 278
Size: 53 83 186 936 1029 204 1333 2468
Fragments arranged by size:
2468 1333 1029 936 204 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 2690 3209 4355 5739 6007 1632
Size: 597 461 519 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 597 519 461 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 5191 6168 1203
Size: 3988 977 1327
Fragments arranged by size:
3988 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2927 3447 1184
Size: 1743 520 4029
Fragments arranged by size:
4029 1743 520
AlwNI CAG_nnn'CTG
Cuts at: 4035 5607 4035
Size: 1572 4720
ApaI G_GGCC'C
Cuts at: 2012 3112 2012
Size: 1100 5192
ApaLI G'TGCA_C
Cuts at: 2516 3059 3422 4349 5505 2516
Size: 543 363 927 1156 3303
Fragments arranged by size:
3303 1156 927 543 363
ApoI r'AATT_y
Cuts at: 1493 3601 3827 3979 6088 1493
Size: 2108 226 152 2109 1697
Fragments arranged by size:
2109 2108 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2225 2225
Size: 6292
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3617 1870
Size: 73 1674 4545
Fragments arranged by size:
4545 1674 73
BamHI G'GATC_C
Cuts at: 2427 3622 3989 2427
Size: 1195 367 4730
Fragments arranged by size:
4730 1195 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2233 2524 2717 3305 3613
Size: 325 217 627 446 291 193 588 308
Cuts at: 3613 5068 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 588 446 325 308 291
217 193
BanII G_rGCy'C
Cuts at: 1391 2012 2113 2549 3112 3171 3611 1391
Size: 621 101 436 563 59 440 4072
Fragments arranged by size:
4072 621 563 440 436 101 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2854 2923 3037 928
Size: 971 955 69 114 4183
Fragments arranged by size:
4183 971 955 114 69
BbvCI CC'TCA_GC
Cuts at: 3357 3357
Size: 6292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3133 4903 136
Size: 107 122 71 631 605 1461 1770 1525
Fragments arranged by size:
1770 1525 1461 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2754 6287 2754
Size: 3533 2759
BlpI GC'TnA_GC
Cuts at: 2045 2091 2283 3194 3709 2045
Size: 46 192 911 515 4628
Fragments arranged by size:
4628 911 515 192 46
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2238 2513 2520 2529 2720 3063 3112
Size: 222 226 275 7 9 191 343 49
Cuts at: 3112 3129 3308 3426 4353 5509 1790
Size: 17 179 118 927 1156 2573
Fragments arranged by size:
2573 1156 927 343 275 226 222 191
179 118 49 17 9 7
BmgBI CAC'GTC
Cuts at: 1318 2251 1318
Size: 933 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 2530 2689
Size: 416 53 83 399 24 713 400 159
Cuts at: 2689 3106 4023 4981 442
Size: 417 917 958 1753
Fragments arranged by size:
1753 958 917 713 417 416 400 399
159 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2141
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2136 2663 3236 4952 1239
Size: 897 527 573 1716 2579
Fragments arranged by size:
2579 1716 897 573 527
Bpu10I CC'TnA_GC
Cuts at: 3357 3357
Size: 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 4955 882
Size: 617 613 2843 2219
Fragments arranged by size:
2843 2219 617 613
BsaAI yAC'GTr
Cuts at: 493 1204 4083 493
Size: 711 2879 2702
Fragments arranged by size:
2879 2702 711
BsaBI GATnn'nnATC
Cuts at: 2426 6286 2426
Size: 3860 2432
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2562 2766 4099 4481
Size: 53 83 186 936 1029 204 1333 382
Cuts at: 4481 5116 275
Size: 635 1451
Fragments arranged by size:
1451 1333 1029 936 635 382 204 186
83 53
BsaWI w'CCGG_w
Cuts at: 4725 5123 5397 5544 5955 4725
Size: 398 274 147 411 5062
Fragments arranged by size:
5062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2486 2516 2531
Size: 30 165 30 708 30 345 30 15
Cuts at: 2531 2561 2858 2888 3053 3083 1178
Size: 30 297 30 165 30 4387
Fragments arranged by size:
4387 708 345 297 165 165 30 30
30 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2119 3251 3272 3275 1186
Size: 933 1132 21 3 4203
Fragments arranged by size:
4203 1132 933 21 3
BseYI C'CCAG_C
Cuts at: 2071 2220 3074 5495 5991 2071
Size: 149 854 2421 496 2372
Fragments arranged by size:
2421 2372 854 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3300 3314 1163
Size: 573 1564 14 4141
Fragments arranged by size:
4141 1564 573 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 2549 3054 3063 3171 3426 4353 4438 5509
Size: 29 505 9 108 255 927 85 1071
Cuts at: 5509 2520
Size: 3303
Fragments arranged by size:
3303 1071 927 505 255 108 85 29
9
BsmI GAATG_Cn'
Cuts at: 3803 3896 3803
Size: 93 6199
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2113 2238 2513 2520 2529 2549
Size: 399 222 101 125 275 7 9 20
Cuts at: 2549 2720 3054 3063 3112 3129 3171 3308 3426
Size: 171 334 9 49 17 42 137 118
Cuts at: 3426 3611 4353 4438 5509 1391
Size: 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 275 222 185
171 137 125 118 101 85 49 42
20 17 9 9 7
BspEI T'CCGG_A
Cuts at: 5955 5955
Size: 6292
BspHI T'CATG_A
Cuts at: 4181 5911 4181
Size: 1730 4562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2066 2877 3006 3272 3336 3450 3647 844
Size: 1222 811 129 266 64 114 197 3489
Fragments arranged by size:
3489 1222 811 266 197 129 114 64
BsrBI CCG'CTC
Cuts at: 1394 1823 2688 2800 2983 3069 4179 1394
Size: 429 865 112 183 86 1110 3507
Fragments arranged by size:
3507 1110 865 429 183 112 86
BsrDI GCAATG_nn'
Cuts at: 65 1248 2147 3429 4787 4961 65
Size: 1183 899 1282 1358 174 1396
Fragments arranged by size:
1396 1358 1282 1183 899 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 3147 4936 5123 1700
Size: 110 374 963 1789 187 2869
Fragments arranged by size:
2869 1789 963 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 2225 1925
Size: 300 5992
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 3312 794
Size: 1730 788 3774
Fragments arranged by size:
3774 1730 788
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 6292
BstYI r'GATC_y
Cuts at: 1972 2427 2754 3510 3622 3989 4374 4391 5843
Size: 455 327 756 112 367 385 17 1452
Cuts at: 5843 5929 5941 6287 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 756 455 385 367 346 327
112 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3343 3343
Size: 6292
BtgI C'CryG_G
Cuts at: 513 1065 2004 2142 2505 2712 3162 3319 3495
Size: 552 939 138 363 207 450 157 176
Cuts at: 3495 513
Size: 3310
Fragments arranged by size:
3310 939 552 450 363 207 176 157
138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2263 487
Size: 683 56 1037 4516
Fragments arranged by size:
4516 1037 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3808 4602 4622 2478
Size: 1330 794 20 4148
Fragments arranged by size:
4148 1330 794 20
ClaI AT'CG_AT
Cuts at: 1554 2292 1554
Size: 738 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2365 2400 529
Size: 35 1801 35 4421
Fragments arranged by size:
4421 1801 35 35
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DraIII CAC_nnn'GTG
Cuts at: 2524 2901 3100 2524
Size: 377 199 5716
Fragments arranged by size:
5716 377 199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 3322 3739
Size: 54 1425 50 275 18 702 790 417
Cuts at: 3739 4628 6257 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 790 702 417 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 2207 2504 2807 3469 4222 1353
Size: 842 12 297 303 662 753 3423
Fragments arranged by size:
3423 842 753 662 303 297 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2547 3169 2547
Size: 622 5670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2136 2229 2663 2690 3209 3236 4355
Size: 393 504 93 434 27 519 27 1119
Cuts at: 4355 4952 5739 6007 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1119 787 597 519 504 434 393
268 93 27 27
EcoO109I rG'GnC_Cy
Cuts at: 2009 2407 3108 3109 3339 3347 2009
Size: 398 701 1 230 8 4954
Fragments arranged by size:
4954 701 398 230 8 1
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2098 5439 1678
Size: 420 3341 2531
Fragments arranged by size:
3341 2531 420
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2262 2294 3446
Size: 32 135 32 539 32 119 32 1152
Cuts at: 3446 3478 4933 4965 5007 5039 1373
Size: 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1455 1152 539 135 119 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3636 3882 669
Size: 988 281 1698 246 3079
Fragments arranged by size:
3079 1698 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3891 3891
Size: 6292
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2901 5406 5590 1305
Size: 1596 2505 184 2007
Fragments arranged by size:
2505 2007 1596 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4470
Fragments arranged by size:
4470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2909 4250 4609 4768 518
Size: 648 1128 615 1341 359 159 2042
Fragments arranged by size:
2042 1341 1128 648 615 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 3708 4385 5533 5778 1606
Size: 614 1488 677 1148 245 2120
Fragments arranged by size:
2120 1488 1148 677 614 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3495 513
Size: 552 939 1491 3310
Fragments arranged by size:
3310 1491 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3279 3403 3650 5195 1132
Size: 97 2050 124 247 1545 2229
Fragments arranged by size:
2229 2050 1545 247 124 97
PasI CC'CwG_GG
Cuts at: 1348 2106 1348
Size: 758 5534
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2078 2331 1108
Size: 970 253 5069
Fragments arranged by size:
5069 970 253
PfoI T'CCnGG_A
Cuts at: 2113 2612 2113
Size: 499 5793
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2407 3347 2407
Size: 940 5352
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PspOMI G'GGCC_C
Cuts at: 2008 3108 2008
Size: 1100 5192
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 6260
PstI C_TGCA'G
Cuts at: 830 2555 3317 3372 3644 830
Size: 1725 762 55 272 3478
Fragments arranged by size:
3478 1725 762 272 55
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
SacI G_AGCT'C
Cuts at: 2549 3171 2549
Size: 622 5670
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SanDI GG'GwC_CC
Cuts at: 2407 2407
Size: 6292
SapI GCTCTTCn'nnn_
Cuts at: 2195 2807 2195
Size: 612 5680
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2551 3313 3368 3640 4775 5456 5647
Size: 219 1506 762 55 272 1135 681 191
Cuts at: 5647 826
Size: 1471
Fragments arranged by size:
1506 1471 1135 762 681 272 219 191
55
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6292
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 3351 3495 3731 513
Size: 552 939 483 864 144 236 3074
Fragments arranged by size:
3074 939 864 552 483 236 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2512 3088 3095 4319 4336 4489 4674
Size: 1255 932 576 7 1224 17 153 185
Cuts at: 4674 5119 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1224 932 576 445 185 153
17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4538 6250 96
Size: 274 80 33 51 1443 2561 1712 138
Fragments arranged by size:
2561 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 3053 3085 6252 6284 2486
Size: 32 535 32 3167 32 2494
Fragments arranged by size:
3167 2494 535 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 3332 2638
Size: 694 5598
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 4421 4421
Size: 6292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2563 2767 4100 276
Size: 53 83 186 936 1029 204 1333 2468
Fragments arranged by size:
2468 1333 1029 936 204 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII
RsrII SacII SexAI SfiI SfoI SrfI SwaI