Restriction map for
pFN21AE2450
(Circular) (Six-base) MAPSORT of: pFN21AE2450.seq Check: 8501 from: 1 to: 5668
pFN21AE2450 5668 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2329 2989 2329
Size: 660 5008
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3731 5115 5383 1632
Size: 2099 1384 268 1917
Fragments arranged by size:
2099 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 4567 5544 1203
Size: 3364 977 1327
Fragments arranged by size:
3364 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 3411 4983 3411
Size: 1572 4096
ApaLI G'TGCA_C
Cuts at: 2645 3725 4881 2645
Size: 1080 1156 3432
Fragments arranged by size:
3432 1156 1080
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2504 2741 2993 1870
Size: 73 561 237 252 4545
Fragments arranged by size:
4545 561 252 237 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 2584 2989 4444 618
Size: 325 217 627 542 255 405 1455 1842
Fragments arranged by size:
1842 1455 627 542 405 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2109 2987 1391
Size: 718 878 4072
Fragments arranged by size:
4072 878 718
BbeI G_GCGC'C
Cuts at: 2588 2588
Size: 5668
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2275 2884 928
Size: 971 376 609 3712
Fragments arranged by size:
3712 971 609 376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3859 3893 1903
Size: 34 50 34 1838 34 3678
Fragments arranged by size:
3678 1838 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 2364 1548
Size: 816 4852
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2640 4279 136
Size: 107 122 71 631 605 968 1639 1525
Fragments arranged by size:
1639 1525 968 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2649 3729 4885 1790
Size: 859 1080 1156 2573
Fragments arranged by size:
2573 1156 1080 859
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5668
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2338 3399 4357
Size: 416 53 83 399 24 921 1061 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1061 958 921 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2792 4328 1239
Size: 1553 1536 2579
Fragments arranged by size:
2579 1553 1536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2585 3475 3857 4492
Size: 53 83 186 936 1052 890 382 635
Cuts at: 4492 275
Size: 1451
Fragments arranged by size:
1451 1052 936 890 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2936 2966 1178
Size: 30 165 30 1533 30 3880
Fragments arranged by size:
3880 1533 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 2789 1186
Size: 903 700 4065
Fragments arranged by size:
4065 903 700
BseYI C'CCAG_C
Cuts at: 4871 5367 4871
Size: 496 5172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2708 2890 1163
Size: 573 972 182 3941
Fragments arranged by size:
3941 972 573 182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2649 2718 3729 3814 4885 2190
Size: 459 69 1011 85 1071 2973
Fragments arranged by size:
2973 1071 1011 459 85 69
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2190 2649 2718 2987 3729 3814
Size: 399 319 81 459 69 269 742 85
Cuts at: 3814 4885 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 319 269 85
81 69
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2178 2619 3023 844
Size: 1334 441 404 3489
Fragments arranged by size:
3489 1334 441 404
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 4163 4337 65
Size: 1183 1539 1376 174 1396
Fragments arranged by size:
1539 1396 1376 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4312 4499 1700
Size: 110 2502 187 2869
Fragments arranged by size:
2869 2502 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 2294 3728 4740 2294
Size: 1434 1012 3222
Fragments arranged by size:
3222 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2694 2722 794
Size: 1900 28 3740
Fragments arranged by size:
3740 1900 28
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstXI CCAn_nnnn'nTGG
Cuts at: 2770 2941 2770
Size: 171 5497
BstYI r'GATC_y
Cuts at: 1972 2766 2998 3365 3750 3767 5219 5305 5317
Size: 794 232 367 385 17 1452 86 12
Cuts at: 5317 5663 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 794 385 367 346 232 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2580 2629 513
Size: 552 1515 49 3552
Fragments arranged by size:
3552 1515 552 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3978 3998 3184
Size: 794 20 4854
Fragments arranged by size:
4854 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2559 2683 2760
Size: 54 1425 50 275 18 729 124 77
Cuts at: 2760 3115 4004 5633 8
Size: 355 889 1629 43
Fragments arranged by size:
1629 1425 889 729 355 275 124 77
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 3598 1353
Size: 1139 1106 3423
Fragments arranged by size:
3423 1139 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2185 4255 4629 4775 387
Size: 1064 138 596 2070 374 146 1280
Fragments arranged by size:
2070 1280 1064 596 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2792 3731 4328 5115 5383 1239
Size: 393 1160 939 597 787 268 1524
Fragments arranged by size:
1524 1160 939 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2774 2774
Size: 5668
EcoO109I rG'GnC_Cy
Cuts at: 2050 2749 2050
Size: 699 4969
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
EcoRV GAT'ATC
Cuts at: 2020 2020
Size: 5668
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2551 2580 2588 4815 1678
Size: 873 29 8 2227 2531
Fragments arranged by size:
2531 2227 873 29 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2914 2936 2946 2968 4309
Size: 32 135 32 1342 22 10 22 1341
Cuts at: 4309 4341 4383 4415 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1342 1341 135 42 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KasI G'GCGC_C
Cuts at: 2584 2584
Size: 5668
KpnI G_GTAC'C
Cuts at: 2333 2993 2333
Size: 660 5008
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4782 4966 1305
Size: 3477 184 2007
Fragments arranged by size:
3477 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2561 2762 10
Size: 54 1425 343 729 201 2916
Fragments arranged by size:
2916 1425 729 343 201 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3626 3985 4144 518
Size: 648 1755 705 359 159 2042
Fragments arranged by size:
2042 1755 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2545 2631 2805 3084 3761 4909 5154 1606
Size: 939 86 174 279 677 1148 245 2120
Fragments arranged by size:
2120 1148 939 677 279 245 174 86
NarI GG'CG_CC
Cuts at: 2585 2585
Size: 5668
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5116
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2251 1348
Size: 903 4765
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PfoI T'CCnGG_A
Cuts at: 2769 2769
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 3737 3769 5250 5282 2828
Size: 32 877 32 1481 32 3214
Fragments arranged by size:
3214 1481 877 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2749 2050
Size: 699 4969
PshAI GACnn'nnGTC
Cuts at: 1613 2456 1613
Size: 843 4825
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 5636
PstI C_TGCA'G
Cuts at: 830 2230 2409 2555 2834 3020 830
Size: 1400 179 146 279 186 3478
Fragments arranged by size:
3478 1400 279 186 179 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SacII CC_GC'GG
Cuts at: 2632 2632
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SanDI GG'GwC_CC
Cuts at: 2749 2749
Size: 5668
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 2348 3916 5628 2348
Size: 1568 1712 2388
Fragments arranged by size:
2388 1712 1568
SfcI C'TryA_G
Cuts at: 826 1045 2226 2405 2551 2830 3016 4151 4832
Size: 219 1181 179 146 279 186 1135 681
Cuts at: 4832 5023 826
Size: 191 1471
Fragments arranged by size:
1471 1181 1135 681 279 219 191 186
179 146
SfoI GGC'GCC
Cuts at: 2586 2586
Size: 5668
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2506 2995 1872
Size: 634 489 4545
Fragments arranged by size:
4545 634 489
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3772 4673 4935 820
Size: 197 926 1829 901 262 1553
Fragments arranged by size:
1829 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 3107 513
Size: 552 2042 3074
Fragments arranged by size:
3074 2042 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2878 3695 3712 3865 4050 4495 325
Size: 1255 1298 817 17 153 185 445 1498
Fragments arranged by size:
1498 1298 1255 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 2616 3914
Size: 274 80 33 51 1443 369 270 1298
Cuts at: 3914 5626 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1298 369 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2855 2887 5628 5660 2855
Size: 32 2741 32 2863
Fragments arranged by size:
2863 2741 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 2368
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5668
XmaI C'CCGG_G
Cuts at: 1870 2504 2993 1870
Size: 634 489 4545
Fragments arranged by size:
4545 634 489
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PvuII RsrII SacI SapI
SexAI SfiI SrfI SwaI