Restriction map for
pFN21AE2449
(Circular) (Six-base) MAPSORT of: pFN21AE2449.seq Check: 9878 from: 1 to: 7525
pFN21AE2449 7525 bp
With 182 enzymes: SgfI *
January 18, 2013 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5335 278
Size: 53 83 186 936 3799 2468
Fragments arranged by size:
3799 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3402 4868 1937
Size: 1465 1466 4594
Fragments arranged by size:
4594 1466 1465
Acc65I G'GTAC_C
Cuts at: 4846 4846
Size: 7525
AclI AA'CG_TT
Cuts at: 1563 3786 5652 6025 1563
Size: 2223 1866 373 3063
Fragments arranged by size:
3063 2223 1866 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 3270 3598 4385 5588 6972 7240 1632
Size: 846 792 328 787 1203 1384 268 1917
Fragments arranged by size:
1917 1384 1203 846 792 787 328 268
AflII C'TTAA_G
Cuts at: 820 1017 2866 4411 820
Size: 197 1849 1545 3934
Fragments arranged by size:
3934 1849 1545 197
AflIII A'CryG_T
Cuts at: 1203 2572 3654 6424 7401 1203
Size: 1369 1082 2770 977 1327
Fragments arranged by size:
2770 1369 1327 1082 977
AgeI A'CCGG_T
Cuts at: 6356 6356
Size: 7525
AhdI GACnn_n'nnGTC
Cuts at: 2664 6254 2664
Size: 3590 3935
AleI CACnn'nnGTG
Cuts at: 1166 2738 4620 1166
Size: 1572 1882 4071
Fragments arranged by size:
4071 1882 1572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2390 2756 1184
Size: 1206 366 5953
Fragments arranged by size:
5953 1206 366
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 3937 3969 2163
Size: 32 1742 32 5719
Fragments arranged by size:
5719 1742 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5268 6840 5268
Size: 1572 5953
ApaLI G'TGCA_C
Cuts at: 5582 6738 5582
Size: 1156 6369
ApoI r'AATT_y
Cuts at: 1493 2199 4738 4802 4834 5060 5212 7321 1493
Size: 706 2539 64 32 226 152 2109 1697
Fragments arranged by size:
2539 2109 1697 706 226 152 64 32
AseI AT'TA_AT
Cuts at: 160 1032 2883 4449 6079 160
Size: 872 1851 1566 1630 1606
Fragments arranged by size:
1851 1630 1606 1566 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7525
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 4161 4850 1870
Size: 73 187 2031 689 4545
Fragments arranged by size:
4545 2031 689 187 73
BamHI G'GATC_C
Cuts at: 2013 4855 5222 2013
Size: 2842 367 4316
Fragments arranged by size:
4316 2842 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 4002 4846 6301 618
Size: 325 217 627 660 1555 844 1455 1842
Fragments arranged by size:
1842 1555 1455 844 660 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2509 2595 2935 4110 4420 4844 1391
Size: 1118 86 340 1175 310 424 4072
Fragments arranged by size:
4072 1175 1118 424 340 310 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2151 2438 2822 3096 3258 3447 4226
Size: 971 252 287 384 274 162 189 779
Cuts at: 4226 4509 928
Size: 283 3944
Fragments arranged by size:
3944 971 779 384 287 283 274 252
189 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5716 5750 1903
Size: 34 3779 34 3678
Fragments arranged by size:
3779 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5417 6627 5417
Size: 1210 6315
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7525
BfrBI ATG'CAT
Cuts at: 4470 4470
Size: 7525
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2383 3722 6136
Size: 107 122 71 631 605 711 1339 2414
Cuts at: 6136 136
Size: 1525
Fragments arranged by size:
2414 1525 1339 711 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3545 7520 3545
Size: 3975 3550
BlpI GC'TnA_GC
Cuts at: 4942 4942
Size: 7525
Bme1580I G_kGCm'C
Cuts at: 1790 2722 4007 5586 6742 1790
Size: 932 1285 1579 1156 2573
Fragments arranged by size:
2573 1579 1285 1156 932
BmgBI CAC'GTC
Cuts at: 1318 2573 1318
Size: 1255 6270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3416 3655 4772
Size: 416 53 83 399 24 1999 239 1117
Cuts at: 4772 5256 6214 442
Size: 484 958 1753
Fragments arranged by size:
1999 1753 1117 958 484 416 399 239
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4319
Size: 7525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3183 3300 3939 6185 1239
Size: 1944 117 639 2246 2579
Fragments arranged by size:
2579 2246 1944 639 117
Bpu10I CC'TnA_GC
Cuts at: 2499 3407 2499
Size: 908 6617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 5650 6515 6813 2285
Size: 3365 865 298 2997
Fragments arranged by size:
3365 2997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3022 4290 6188 882
Size: 617 1523 1268 1898 2219
Fragments arranged by size:
2219 1898 1523 1268 617
BsaAI yAC'GTr
Cuts at: 493 1204 5316 493
Size: 711 4112 2702
Fragments arranged by size:
4112 2702 711
BsaBI GATnn'nnATC
Cuts at: 7519 7519
Size: 7525
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3331 5332 5714 6349
Size: 53 83 186 936 1798 2001 382 635
Cuts at: 6349 275
Size: 1451
Fragments arranged by size:
2001 1798 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4212 5958 6356 6630 6777 7188 4212
Size: 1746 398 274 147 411 4549
Fragments arranged by size:
4549 1746 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3280 3310 3937 3967 1178
Size: 30 165 30 1877 30 627 30 4736
Fragments arranged by size:
4736 1877 627 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2170 2303 2455 3564 1186
Size: 886 98 133 152 1109 5147
Fragments arranged by size:
5147 1109 886 152 133 98
BseYI C'CCAG_C
Cuts at: 2612 4338 6728 7224 2612
Size: 1726 2390 496 2913
Fragments arranged by size:
2913 2390 1726 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2376 3483 4598 1163
Size: 573 640 1107 1115 4090
Fragments arranged by size:
4090 1115 1107 640 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4975 5736 6764 1540
Size: 275 186 2974 761 1028 2301
Fragments arranged by size:
2974 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2301 3733 4110 5586 5671 6742 2301
Size: 1432 377 1476 85 1071 3084
Fragments arranged by size:
3084 1476 1432 1071 377 85
BsmI GAATG_Cn'
Cuts at: 2356 3742 5036 5129 2356
Size: 1386 1294 93 4752
Fragments arranged by size:
4752 1386 1294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2509 2595 2722 2935 3733 4007
Size: 399 511 208 86 127 213 798 274
Cuts at: 4007 4110 4420 4844 5586 5671 6742 1391
Size: 103 310 424 742 85 1071 2174
Fragments arranged by size:
2174 1071 798 742 511 424 399 310
274 213 208 127 103 86 85
BspEI T'CCGG_A
Cuts at: 7188 7188
Size: 7525
BspHI T'CATG_A
Cuts at: 5414 7144 5414
Size: 1730 5795
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3460 4880 844
Size: 2616 1420 3489
Fragments arranged by size:
3489 2616 1420
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 4231 5412 1394
Size: 429 341 2067 1181 3507
Fragments arranged by size:
3507 2067 1181 429 341
BsrDI GCAATG_nn'
Cuts at: 65 1248 3963 6020 6194 65
Size: 1183 2715 2057 174 1396
Fragments arranged by size:
2715 2057 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6169 6356 1700
Size: 110 4359 187 2869
Fragments arranged by size:
4359 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7525
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7525
BssSI C'ACGA_G
Cuts at: 5585 6597 5585
Size: 1012 6513
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2726 3737 794
Size: 1932 1011 4582
Fragments arranged by size:
4582 1932 1011
BstBI TT'CG_AA
Cuts at: 6329 6329
Size: 7525
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7525
BstYI r'GATC_y
Cuts at: 1972 2013 3545 4752 4855 5222 5607 5624 7076
Size: 41 1532 1207 103 367 385 17 1452
Cuts at: 7076 7162 7174 7520 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1532 1452 1207 385 367 346 103
86 41 17 12
Bsu36I CC'TnA_GG
Cuts at: 2237 2237
Size: 7525
BtgI C'CryG_G
Cuts at: 513 1065 2760 3349 4291 513
Size: 552 1695 589 942 3747
Fragments arranged by size:
3747 1695 942 589 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4524 487
Size: 683 56 3298 3488
Fragments arranged by size:
3488 3298 683 56
BtsI GCAGTG_nn'
Cuts at: 2623 2745 5041 5835 5855 2623
Size: 122 2296 794 20 4293
Fragments arranged by size:
4293 2296 794 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7525
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7490
DraI TTT'AAA
Cuts at: 2583 4829 2583
Size: 2246 5279
DraIII CAC_nnn'GTG
Cuts at: 3271 3271
Size: 7525
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6532 809
Size: 507 5216 1802
Fragments arranged by size:
5216 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4288 4972 5861
Size: 54 1425 50 275 18 2458 684 889
Cuts at: 5861 7490 8
Size: 1629 43
Fragments arranged by size:
2458 1629 1425 889 684 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4972 1537
Size: 275 3160 4090
Fragments arranged by size:
4090 3160 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 2985 3115 3810 5455 1353
Size: 1241 391 130 695 1645 3423
Fragments arranged by size:
3423 1645 1241 695 391 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 6112 6486 6632 387
Size: 1064 138 504 4019 374 146 1280
Fragments arranged by size:
4019 1280 1064 504 374 146 138
EcoICRI GAG'CTC
Cuts at: 4108 4108
Size: 7525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 3183 3270 3300 3598 3939 4385
Size: 393 846 705 87 30 298 341 446
Cuts at: 4385 5588 6185 6972 7240 1239
Size: 1203 597 787 268 1524
Fragments arranged by size:
1524 1203 846 787 705 597 446 393
341 298 268 87 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3587 4596 3587
Size: 1009 6516
EcoO109I rG'GnC_Cy
Cuts at: 3304 3304
Size: 7525
EcoRI G'AATT_C
Cuts at: 4834 5212 4834
Size: 378 7147
FspI TGC'GCA
Cuts at: 6031 6031
Size: 7525
HaeII r_GCGC'y
Cuts at: 1678 6672 1678
Size: 4994 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2215 2247 2593
Size: 32 135 32 541 32 70 32 346
Cuts at: 2593 2625 2650 2682 3280 3312 6166 6198 6240
Size: 32 25 32 598 32 2854 32 42
Cuts at: 6240 6272 1373
Size: 32 2626
Fragments arranged by size:
2854 2626 598 541 346 135 70 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4869 5115 669
Size: 988 281 1818 1113 246 3079
Fragments arranged by size:
3079 1818 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7525
HpaI GTT'AAC
Cuts at: 5115 5115
Size: 7525
KpnI G_GTAC'C
Cuts at: 4850 4850
Size: 7525
MfeI C'AATT_G
Cuts at: 5124 5124
Size: 7525
MluI A'CGCG_T
Cuts at: 7401 7401
Size: 7525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3777 3920 6639 6823 1305
Size: 2472 143 2719 184 2007
Fragments arranged by size:
2719 2472 2007 184 143
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4290 10
Size: 54 1425 343 2458 3245
Fragments arranged by size:
3245 2458 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2738 3890 3917 4620 5483 5842 6001
Size: 648 1572 1152 27 703 863 359 159
Cuts at: 6001 518
Size: 2042
Fragments arranged by size:
2042 1572 1152 863 703 648 359 159
27
MspA1I CmG'CkG
Cuts at: 1606 2639 4941 5618 6766 7011 1606
Size: 1033 2302 677 1148 245 2120
Fragments arranged by size:
2302 2120 1148 1033 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2760 3349 4291 513
Size: 552 1695 589 942 3747
Fragments arranged by size:
3747 1695 942 589 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7525
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7525
NotI GC'GGCC_GC
Cuts at: 4972 4972
Size: 7525
NsiI A_TGCA'T
Cuts at: 4472 4472
Size: 7525
NspI r_CATG'y
Cuts at: 1132 1229 3658 4883 6428 1132
Size: 97 2429 1225 1545 2229
Fragments arranged by size:
2429 2229 1545 1225 97
PasI CC'CwG_GG
Cuts at: 1348 2356 1348
Size: 1008 6517
PciI A'CATG_T
Cuts at: 3654 6424 3654
Size: 2770 4755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7525
PfoI T'CCnGG_A
Cuts at: 3083 3083
Size: 7525
PmeI GTTT'AAAC
Cuts at: 4829 4829
Size: 7525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3937 3969 5594 5626 7107 7139 3937
Size: 32 1625 32 1481 32 4323
Fragments arranged by size:
4323 1625 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3304 3304
Size: 7525
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7525
PsiI TTA'TAA
Cuts at: 2978 5095 2978
Size: 2117 5408
PstI C_TGCA'G
Cuts at: 830 2403 4877 830
Size: 1573 2474 3478
Fragments arranged by size:
3478 2474 1573
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7525
PvuII CAG'CTG
Cuts at: 2639 2639
Size: 7525
SacI G_AGCT'C
Cuts at: 4110 4110
Size: 7525
SalI G'TCGA_C
Cuts at: 1936 4867 1936
Size: 2931 4594
SanDI GG'GwC_CC
Cuts at: 3304 3304
Size: 7525
SapI GCTCTTCn'nnn_
Cuts at: 2985 2985
Size: 7525
SbfI CC_TGCA'GG
Cuts at: 4877 4877
Size: 7525
ScaI AGT'ACT
Cuts at: 2674 5773 7485 2674
Size: 3099 1712 2714
Fragments arranged by size:
3099 2714 1712
SexAI A'CCwGG_T
Cuts at: 3267 4544 3267
Size: 1277 6248
SfcI C'TryA_G
Cuts at: 826 1045 2399 3210 3336 4488 4873 6008 6689
Size: 219 1354 811 126 1152 385 1135 681
Cuts at: 6689 6880 826
Size: 191 1471
Fragments arranged by size:
1471 1354 1152 1135 811 681 385 219
191 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7525
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7525
SmaI CCC'GGG
Cuts at: 1872 4852 1872
Size: 2980 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2300 2866 4411 5629 6530 6792
Size: 197 926 357 566 1545 1218 901 262
Cuts at: 6792 820
Size: 1553
Fragments arranged by size:
1553 1545 1218 926 901 566 357 262
197
SnaBI TAC'GTA
Cuts at: 493 5316 493
Size: 4823 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7525
SphI G_CATG'C
Cuts at: 1132 4883 1132
Size: 3751 3774
SspI AAT'ATT
Cuts at: 5 52 3042 4814 5449 5
Size: 47 2990 1772 635 2081
Fragments arranged by size:
2990 2081 1772 635 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7525
StyI C'CwwG_G
Cuts at: 513 1065 2191 2760 3299 3349 4291 4312 4964
Size: 552 1126 569 539 50 942 21 652
Cuts at: 4964 513
Size: 3074
Fragments arranged by size:
3074 1126 942 652 569 552 539 50
21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4352 5552 5569 5722 5907 6352 325
Size: 1255 2772 1200 17 153 185 445 1498
Fragments arranged by size:
2772 1498 1255 1200 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2560 2672 5771
Size: 274 80 33 51 1443 583 112 3099
Cuts at: 5771 7483 96
Size: 1712 138
Fragments arranged by size:
3099 1712 1443 583 274 138 112 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3079 3111 3512 3544 4117 4149 7485 7517 3079
Size: 32 401 32 573 32 3336 32 3087
Fragments arranged by size:
3336 3087 573 401 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7525
XbaI T'CTAG_A
Cuts at: 4861 4861
Size: 7525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3158 4794 3158
Size: 1636 5889
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7525
XmaI C'CCGG_G
Cuts at: 1870 4850 1870
Size: 2980 4545
XmnI GAAnn'nnTTC
Cuts at: 3804 4741 5654 3804
Size: 937 913 5675
Fragments arranged by size:
5675 937 913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5333 276
Size: 53 83 186 936 3799 2468
Fragments arranged by size:
3799 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI