Restriction map for
pFN21AE2448
(Circular) (Six-base) MAPSORT of: pFN21AE2448.seq Check: 1502 from: 1 to: 6502
pFN21AE2448 6502 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2280 4312 278
Size: 53 83 186 936 744 2032 2468
Fragments arranged by size:
2468 2032 936 744 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3474 3845 1937
Size: 1537 371 4594
Fragments arranged by size:
4594 1537 371
Acc65I G'GTAC_C
Cuts at: 3387 3823 3387
Size: 436 6066
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 4565 5949 6217 1632
Size: 542 2391 1384 268 1917
Fragments arranged by size:
2391 1917 1384 542 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2083 2115 2083
Size: 32 6470
AlwNI CAG_nnn'CTG
Cuts at: 2302 2868 4245 5817 2302
Size: 566 1377 1572 2987
Fragments arranged by size:
2987 1572 1377 566
ApaLI G'TGCA_C
Cuts at: 2585 2887 4559 5715 2585
Size: 302 1672 1156 3372
Fragments arranged by size:
3372 1672 1156 302
ApoI r'AATT_y
Cuts at: 1493 2555 3811 4037 4189 6298 1493
Size: 1062 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 1062 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 3827 1870
Size: 73 1884 4545
Fragments arranged by size:
4545 1884 73
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2678 2707 2834 2873 3149
Size: 325 217 627 891 29 127 39 276
Cuts at: 3149 3272 3387 3394 3612 3823 5278 618
Size: 123 115 7 218 211 1455 1842
Fragments arranged by size:
1842 1455 891 627 325 276 218 217
211 127 123 115 39 29 7
BanII G_rGCy'C
Cuts at: 1391 2184 2241 2958 3263 3522 3687 3821 1391
Size: 793 57 717 305 259 165 134 4072
Fragments arranged by size:
4072 793 717 305 259 165 134 57
BbeI G_GCGC'C
Cuts at: 2711 2877 3153 3616 2711
Size: 166 276 463 5597
Fragments arranged by size:
5597 463 276 166
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3178 928
Size: 971 1279 4252
Fragments arranged by size:
4252 1279 971
BbvCI CC'TCA_GC
Cuts at: 2148 2282 2148
Size: 134 6368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4693 4727 1903
Size: 34 50 34 2672 34 3678
Fragments arranged by size:
3678 2672 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 2775 3225 1548
Size: 1227 450 4825
Fragments arranged by size:
4825 1227 450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3293 3625 5113
Size: 107 122 71 631 605 1621 332 1488
Cuts at: 5113 136
Size: 1525
Fragments arranged by size:
1621 1525 1488 631 605 332 122 107
71
BglII A'GATC_T
Cuts at: 3780 6497 3780
Size: 2717 3785
BlpI GC'TnA_GC
Cuts at: 2448 3326 3919 2448
Size: 878 593 5031
Fragments arranged by size:
5031 878 593
Bme1580I G_kGCm'C
Cuts at: 1790 2478 2589 2891 2951 3207 4563 5719 1790
Size: 688 111 302 60 256 1356 1156 2573
Fragments arranged by size:
2573 1356 1156 688 302 256 111 60
BmgBI CAC'GTC
Cuts at: 1318 2415 1318
Size: 1097 5405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3266 4233 5191
Size: 416 53 83 399 24 1849 967 958
Cuts at: 5191 442
Size: 1753
Fragments arranged by size:
1849 1753 967 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2672 1056
Size: 1616 4886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2091 2651 2672 3701 5162 1239
Size: 841 11 560 21 1029 1461 2579
Fragments arranged by size:
2579 1461 1029 841 560 21 11
Bpu10I CC'TnA_GC
Cuts at: 2148 2282 2148
Size: 134 6368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2705 4627 5492 5790 2210
Size: 495 1922 865 298 2922
Fragments arranged by size:
2922 1922 865 495 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2530 5165 882
Size: 617 1031 2635 2219
Fragments arranged by size:
2635 2219 1031 617
BsaAI yAC'GTr
Cuts at: 493 1204 4293 493
Size: 711 3089 2702
Fragments arranged by size:
3089 2702 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 2708 2874 3150
Size: 53 83 186 936 744 431 166 276
Cuts at: 3150 3613 4309 4691 5326 275
Size: 463 696 382 635 1451
Fragments arranged by size:
1451 936 744 696 635 463 431 382
276 186 166 83 53
BsaWI w'CCGG_w
Cuts at: 4935 5333 5607 5754 6165 4935
Size: 398 274 147 411 5272
Fragments arranged by size:
5272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6277
Fragments arranged by size:
6277 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 2410 3572 1186
Size: 1045 179 1162 4116
Fragments arranged by size:
4116 1162 1045 179
BseYI C'CCAG_C
Cuts at: 2302 3112 3459 3467 3792 5705 6201 2302
Size: 810 347 8 325 1913 496 2603
Fragments arranged by size:
2603 1913 810 496 347 325 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2420 2777 3384 1163
Size: 573 684 357 607 4281
Fragments arranged by size:
4281 684 607 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3198 3620 3952 4713 5741
Size: 275 186 1060 137 422 332 761 1028
Cuts at: 5741 1540
Size: 2301
Fragments arranged by size:
2301 1060 1028 761 422 332 275 186
137
BsiHKAI G_wGCw'C
Cuts at: 2184 2589 2891 2958 3009 3582 4563 4648 5719
Size: 405 302 67 51 573 981 85 1071
Cuts at: 5719 2184
Size: 2967
Fragments arranged by size:
2967 1071 981 573 405 302 85 67
51
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2184 2241 2478 2589 2891 2951 2958
Size: 399 394 57 237 111 302 60 7
Cuts at: 2958 3009 3207 3263 3522 3582 3687 3821 4563
Size: 51 198 56 259 60 105 134 742
Cuts at: 4563 4648 5719 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 302 259 237
198 134 111 105 85 60 60 57
56 51 7
BspEI T'CCGG_A
Cuts at: 6165 6165
Size: 6502
BspHI T'CATG_A
Cuts at: 2778 4391 6121 2778
Size: 1613 1730 3159
Fragments arranged by size:
3159 1730 1613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3328 3486 3857 844
Size: 2484 158 371 3489
Fragments arranged by size:
3489 2484 371 158
BsrBI CCG'CTC
Cuts at: 1394 1823 2740 4389 1394
Size: 429 917 1649 3507
Fragments arranged by size:
3507 1649 917 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 2213 2990 3152 3371 3615 3619
Size: 110 382 21 777 162 219 244 4
Cuts at: 3619 5146 5333 1700
Size: 1527 187 2869
Fragments arranged by size:
2869 1527 777 382 244 219 187 162
110 21 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6502
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3726 794
Size: 2932 3570
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 3442 3700 1184
Size: 2258 258 3986
Fragments arranged by size:
3986 2258 258
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2485 2521 3421 2359
Size: 126 36 900 5440
Fragments arranged by size:
5440 900 126 36
BstYI r'GATC_y
Cuts at: 1972 2592 3780 3832 4199 4584 4601 6053 6139
Size: 620 1188 52 367 385 17 1452 86
Cuts at: 6139 6151 6497 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1188 620 385 367 346 86
52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2640 2838 2970 513
Size: 552 1575 198 132 4045
Fragments arranged by size:
4045 1575 552 198 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 2791 3167 3401 4018 4812 4832 2791
Size: 376 234 617 794 20 4461
Fragments arranged by size:
4461 794 617 376 234 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2211 2316
Size: 54 1425 50 275 18 360 21 105
Cuts at: 2316 2967 2988 3058 3135 3195 3303 3369 3423
Size: 651 21 70 77 60 108 66 54
Cuts at: 3423 3546 3617 3949 4838 6467 8
Size: 123 71 332 889 1629 43
Fragments arranged by size:
1629 1425 889 651 360 332 275 123
108 105 77 71 70 66 60 54
54 50 43 21 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 3195 3617 3949 1537
Size: 275 1246 137 422 332 4090
Fragments arranged by size:
4090 1246 422 332 275 137
EarI CTCTTCn'nnn_
Cuts at: 1353 4432 1353
Size: 3079 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2278 2930 3626 5089 5463 5609
Size: 1064 138 689 652 696 1463 374 146
Cuts at: 5609 387
Size: 1280
Fragments arranged by size:
1463 1280 1064 696 689 652 374 146
138
EcoICRI GAG'CTC
Cuts at: 2182 2956 2182
Size: 774 5728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2091 2174 2651 2672 3701 4565
Size: 393 448 11 83 477 21 1029 864
Cuts at: 4565 5162 5949 6217 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1029 864 787 597 477 448 393
268 83 21 11
EcoO109I rG'GnC_Cy
Cuts at: 2032 2091 3560 2032
Size: 59 1469 4974
Fragments arranged by size:
4974 1469 59
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FseI GG_CCGG'CC
Cuts at: 3623 3623
Size: 6502
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2640 2711 2877 3153 3333 3616 5649 1678
Size: 962 71 166 276 180 283 2033 2531
Fragments arranged by size:
2531 2033 962 283 276 180 166 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 5143 5175 5217
Size: 32 135 32 559 32 2980 32 42
Cuts at: 5217 5249 1373
Size: 32 2626
Fragments arranged by size:
2980 2626 559 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2494 3846 4092 669
Size: 988 281 121 435 1352 246 3079
Fragments arranged by size:
3079 1352 988 435 281 246 121
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KasI G'GCGC_C
Cuts at: 2707 2873 3149 3612 2707
Size: 166 276 463 5597
Fragments arranged by size:
5597 463 276 166
KpnI G_GTAC'C
Cuts at: 3391 3827 3391
Size: 436 6066
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2742 2793 3288 5616 5800 1305
Size: 1071 366 51 495 2328 184 2007
Fragments arranged by size:
2328 2007 1071 495 366 184 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3305 3425 10
Size: 54 1425 343 1473 120 3087
Fragments arranged by size:
3087 1473 1425 343 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 2483 2777 4460 4819 4978 518
Size: 648 1212 105 294 1683 359 159 2042
Fragments arranged by size:
2042 1683 1212 648 359 294 159 105
MspA1I CmG'CkG
Cuts at: 1606 2152 2299 2574 2868 3112 3421 3459 3551
Size: 546 147 275 294 244 309 38 92
Cuts at: 3551 3610 3708 3918 4595 5743 5988 1606
Size: 59 98 210 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 546 309 294 275 245
244 210 147 98 92 59 38
NaeI GCC'GGC
Cuts at: 2194 2215 2992 3154 3373 3617 3621 2194
Size: 21 777 162 219 244 4 5075
Fragments arranged by size:
5075 777 244 219 162 21 4
NarI GG'CG_CC
Cuts at: 2708 2874 3150 3613 2708
Size: 166 276 463 5597
Fragments arranged by size:
5597 463 276 166
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5950
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NgoMIV G'CCGG_C
Cuts at: 2192 2213 2990 3152 3371 3615 3619 2192
Size: 21 777 162 219 244 4 5075
Fragments arranged by size:
5075 777 244 219 162 21 4
NheI G'CTAG_C
Cuts at: 1052 2668 1052
Size: 1616 4886
NotI GC'GGCC_GC
Cuts at: 3058 3949 3058
Size: 891 5611
NspI r_CATG'y
Cuts at: 1132 1229 3860 5405 1132
Size: 97 2631 1545 2229
Fragments arranged by size:
2631 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2674 2697 3687 1348
Size: 1326 23 990 4163
Fragments arranged by size:
4163 1326 990 23
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 3679 3799 1108
Size: 1194 1377 120 3811
Fragments arranged by size:
3811 1377 1194 120
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2091 3560 2032
Size: 59 1469 4974
Fragments arranged by size:
4974 1469 59
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3174 3339 3609 3731 3854 830
Size: 2344 165 270 122 123 3478
Fragments arranged by size:
3478 2344 270 165 123 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2152 2299 2868 2152
Size: 147 569 5786
Fragments arranged by size:
5786 569 147
SacI G_AGCT'C
Cuts at: 2184 2958 2184
Size: 774 5728
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SanDI GG'GwC_CC
Cuts at: 3560 3560
Size: 6502
SbfI CC_TGCA'GG
Cuts at: 3339 3854 3339
Size: 515 5987
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SexAI A'CCwGG_T
Cuts at: 3390 3390
Size: 6502
SfcI C'TryA_G
Cuts at: 826 1045 3170 3335 3605 3727 3850 4985 5666
Size: 219 2125 165 270 122 123 1135 681
Cuts at: 5666 5857 826
Size: 191 1471
Fragments arranged by size:
2125 1471 1135 681 270 219 191 165
123 122
SfoI GGC'GCC
Cuts at: 2709 2875 3151 3614 2709
Size: 166 276 463 5597
Fragments arranged by size:
5597 463 276 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 2720 4606 5507 5769 820
Size: 197 926 282 495 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 495 282 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 2231 3758 1517
Size: 714 1527 4261
Fragments arranged by size:
4261 1527 714
StyI C'CwwG_G
Cuts at: 513 1065 3941 513
Size: 552 2876 3074
Fragments arranged by size:
3074 2876 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 2666 2710 3451 4529 4546 4699
Size: 1255 897 189 44 741 1078 17 153
Cuts at: 4699 4884 5329 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1078 897 741 445 189 185
153 44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 4748 6460
Size: 274 80 33 51 1443 276 2495 1712
Cuts at: 6460 96
Size: 138
Fragments arranged by size:
2495 1712 1443 276 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2647 3289 2647
Size: 642 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2278 4310 276
Size: 53 83 186 936 744 2032 2468
Fragments arranged by size:
2468 2032 936 744 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SfiI SrfI SwaI