Restriction map for
pFN21AE2447
(Circular) (Six-base) MAPSORT of: pFN21AE2447.seq Check: 4763 from: 1 to: 7261
pFN21AE2447 7261 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3380 3380
Size: 7261
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5071 278
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2473 4382 4604 1937
Size: 536 1909 222 4594
Fragments arranged by size:
4594 1909 536 222
Acc65I G'GTAC_C
Cuts at: 4582 4582
Size: 7261
AclI AA'CG_TT
Cuts at: 1563 5388 5761 1563
Size: 3825 373 3063
Fragments arranged by size:
3825 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2082 2907 3695 4212 4418 5324 6708 6976
Size: 450 825 788 517 206 906 1384 268
Cuts at: 6976 1632
Size: 1917
Fragments arranged by size:
1917 1384 906 825 788 517 450 268
206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7064
AflIII A'CryG_T
Cuts at: 1203 6160 7137 1203
Size: 4957 977 1327
Fragments arranged by size:
4957 1327 977
AgeI A'CCGG_T
Cuts at: 6092 6092
Size: 7261
AhdI GACnn_n'nnGTC
Cuts at: 4435 5990 4435
Size: 1555 5706
AleI CACnn'nnGTG
Cuts at: 1166 4418 1166
Size: 3252 4009
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7261
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3906 3938 3906
Size: 32 7229
AlwNI CAG_nnn'CTG
Cuts at: 5004 6576 5004
Size: 1572 5689
ApaI G_GGCC'C
Cuts at: 2620 2620
Size: 7261
ApaLI G'TGCA_C
Cuts at: 2803 5318 6474 2803
Size: 2515 1156 3590
Fragments arranged by size:
3590 2515 1156
ApoI r'AATT_y
Cuts at: 1493 2068 2335 2401 2738 3123 3609 4053 4311
Size: 575 267 66 337 385 486 444 258
Cuts at: 4311 4510 4570 4796 4948 7057 1493
Size: 199 60 226 152 2109 1697
Fragments arranged by size:
2109 1697 575 486 444 385 337 267
258 226 199 152 66 60
AseI AT'TA_AT
Cuts at: 160 1032 5815 160
Size: 872 4783 1606
Fragments arranged by size:
4783 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
AvaI C'yCGr_G
Cuts at: 1870 1943 3746 4586 1870
Size: 73 1803 840 4545
Fragments arranged by size:
4545 1803 840 73
BamHI G'GATC_C
Cuts at: 2899 4591 4958 2899
Size: 1692 367 5202
Fragments arranged by size:
5202 1692 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4582 6037 618
Size: 325 217 627 2795 1455 1842
Fragments arranged by size:
2795 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2609 2620 2816 4580 1391
Size: 1218 11 196 1764 4072
Fragments arranged by size:
4072 1764 1218 196 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5452 5486 1903
Size: 34 50 34 3431 34 3678
Fragments arranged by size:
3678 3431 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5153 6363 5153
Size: 1210 6051
BclI T'GATC_A
Cuts at: 1548 4048 1548
Size: 2500 4761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5872 136
Size: 107 122 71 631 605 4200 1525
Fragments arranged by size:
4200 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2411 7256 2411
Size: 4845 2416
BlpI GC'TnA_GC
Cuts at: 4678 4678
Size: 7261
Bme1580I G_kGCm'C
Cuts at: 1790 2620 2715 2807 5322 6478 1790
Size: 830 95 92 2515 1156 2573
Fragments arranged by size:
2573 2515 1156 830 95 92
BmgBI CAC'GTC
Cuts at: 1318 4199 1318
Size: 2881 4380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4992 5950
Size: 416 53 83 399 24 1727 1848 958
Cuts at: 5950 442
Size: 1753
Fragments arranged by size:
1848 1753 1727 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2888 3228 4273 4353 4408 4498 4512 5921
Size: 1649 340 1045 80 55 90 14 1409
Cuts at: 5921 1239
Size: 2579
Fragments arranged by size:
2579 1649 1409 1045 340 90 80 55
14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2380 3043 5386 6251 6549 2380
Size: 663 2343 865 298 3092
Fragments arranged by size:
3092 2343 865 663 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5924 882
Size: 617 4425 2219
Fragments arranged by size:
4425 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5052 493
Size: 711 3848 2702
Fragments arranged by size:
3848 2702 711
BsaBI GATnn'nnATC
Cuts at: 2370 4047 7255 2370
Size: 1677 3208 2376
Fragments arranged by size:
3208 2376 1677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5068 5450 6085 275
Size: 53 83 186 936 3535 382 635 1451
Fragments arranged by size:
3535 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2351 5694 6092 6366 6513 6924 2351
Size: 3343 398 274 147 411 2688
Fragments arranged by size:
3343 2688 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2661 2691 1178
Size: 30 165 30 1258 30 5748
Fragments arranged by size:
5748 1258 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3734 1186
Size: 2548 4713
BseYI C'CCAG_C
Cuts at: 6464 6960 6464
Size: 496 6765
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3036 1163
Size: 573 1300 5388
Fragments arranged by size:
5388 1300 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4711 5472 6500 1540
Size: 275 186 2710 761 1028 2301
Fragments arranged by size:
2710 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2807 3435 4396 5322 5407 6478 2807
Size: 628 961 926 85 1071 3590
Fragments arranged by size:
3590 1071 961 926 628 85
BsmI GAATG_Cn'
Cuts at: 2126 4538 4772 4865 2126
Size: 2412 234 93 4522
Fragments arranged by size:
4522 2412 234 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4205 4205
Size: 7261
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2609 2620 2715 2807 2816 3435 4396
Size: 399 819 11 95 92 9 619 961
Cuts at: 4396 4580 5322 5407 6478 1391
Size: 184 742 85 1071 2174
Fragments arranged by size:
2174 1071 961 819 742 619 399 184
95 92 85 11 9
BspEI T'CCGG_A
Cuts at: 6924 6924
Size: 7261
BspHI T'CATG_A
Cuts at: 2916 5150 6880 2916
Size: 2234 1730 3297
Fragments arranged by size:
3297 2234 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3380 4372 4616 844
Size: 2536 992 244 3489
Fragments arranged by size:
3489 2536 992 244
BsrBI CCG'CTC
Cuts at: 1394 1823 2574 5148 1394
Size: 429 751 2574 3507
Fragments arranged by size:
3507 2574 751 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5756 5930 65
Size: 1183 4508 174 1396
Fragments arranged by size:
4508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5905 6092 1700
Size: 110 4095 187 2869
Fragments arranged by size:
4095 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3829 96
Size: 3733 3528
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7261
BssSI C'ACGA_G
Cuts at: 5321 6333 5321
Size: 1012 6249
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7261
BstBI TT'CG_AA
Cuts at: 6065 6065
Size: 7261
BstEII G'GTnAC_C
Cuts at: 1184 2638 3667 1184
Size: 1454 1029 4778
Fragments arranged by size:
4778 1454 1029
BstYI r'GATC_y
Cuts at: 1972 2299 2411 2490 2899 4591 4958 5343 5360
Size: 327 112 79 409 1692 367 385 17
Cuts at: 5360 6812 6898 6910 7256 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1692 1452 409 385 367 346 327
112 86 79 17 12
BstZ17I GTA'TAC
Cuts at: 2474 4383 2474
Size: 1909 5352
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6522
Fragments arranged by size:
6522 683 56
BtsI GCAGTG_nn'
Cuts at: 3687 3832 4777 5571 5591 3687
Size: 145 945 794 20 5357
Fragments arranged by size:
5357 945 794 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7261
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3100 3135 529
Size: 35 2536 35 4655
Fragments arranged by size:
4655 2536 35 35
DraI TTT'AAA
Cuts at: 2965 3933 4565 2965
Size: 968 632 5661
Fragments arranged by size:
5661 968 632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6268 809
Size: 507 4952 1802
Fragments arranged by size:
4952 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2150 4708 5597
Size: 54 1425 50 275 18 320 2558 889
Cuts at: 5597 7226 8
Size: 1629 43
Fragments arranged by size:
2558 1629 1425 889 320 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4708 1537
Size: 275 2896 4090
Fragments arranged by size:
4090 2896 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2264 3072 4170 5191 1353
Size: 911 808 1098 1021 3423
Fragments arranged by size:
3423 1098 1021 911 808
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2763 5848 6222 6368 387
Size: 1064 138 1174 3085 374 146 1280
Fragments arranged by size:
3085 1280 1174 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 2888 2907 3228 3695 4212 4273
Size: 393 450 806 19 321 467 517 61
Cuts at: 4273 4353 4408 4418 4498 4512 5324 5921 6708
Size: 80 55 10 80 14 812 597 787
Cuts at: 6708 6976 1239
Size: 268 1524
Fragments arranged by size:
1524 812 806 787 597 517 467 450
393 321 268 80 80 61 55 19
14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4320 4320
Size: 7261
EcoO109I rG'GnC_Cy
Cuts at: 2128 2957 2128
Size: 829 6432
EcoRI G'AATT_C
Cuts at: 2738 4570 4948 2738
Size: 1832 378 5051
Fragments arranged by size:
5051 1832 378
FspI TGC'GCA
Cuts at: 5767 5767
Size: 7261
HaeII r_GCGC'y
Cuts at: 1678 6408 1678
Size: 4730 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4055 4087 4421 4453 5902
Size: 32 135 32 2483 32 334 32 1449
Cuts at: 5902 5934 5976 6008 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2483 1449 334 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2558 4605 4851 669
Size: 988 281 620 2047 246 3079
Fragments arranged by size:
3079 2047 988 620 281 246
HindIII A'AGCT_T
Cuts at: 748 4238 748
Size: 3490 3771
HpaI GTT'AAC
Cuts at: 4851 4851
Size: 7261
KpnI G_GTAC'C
Cuts at: 4586 4586
Size: 7261
MfeI C'AATT_G
Cuts at: 2146 3117 4860 2146
Size: 971 1743 4547
Fragments arranged by size:
4547 1743 971
MluI A'CGCG_T
Cuts at: 7137 7137
Size: 7261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2802 3363 3548 6375 6559 1305
Size: 1497 561 185 2827 184 2007
Fragments arranged by size:
2827 2007 1497 561 185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5439
Fragments arranged by size:
5439 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4418 5219 5578 5737 518
Size: 648 3252 801 359 159 2042
Fragments arranged by size:
3252 2042 801 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2566 3080 4232 4677 5354 6502 6747 1606
Size: 960 514 1152 445 677 1148 245 2120
Fragments arranged by size:
2120 1152 1148 960 677 514 445 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6709
NdeI CA'TA_TG
Cuts at: 387 4306 387
Size: 3919 3342
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7261
NotI GC'GGCC_GC
Cuts at: 4708 4708
Size: 7261
NspI r_CATG'y
Cuts at: 1132 1229 2462 2785 4619 6164 1132
Size: 97 1233 323 1834 1545 2229
Fragments arranged by size:
2229 1834 1545 1233 323 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7261
PciI A'CATG_T
Cuts at: 6160 6160
Size: 7261
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3090 1108
Size: 1982 5279
PmeI GTTT'AAAC
Cuts at: 4565 4565
Size: 7261
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5330 5362 6843 6875 5330
Size: 32 1481 32 5716
Fragments arranged by size:
5716 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2128
Size: 7261
PshAI GACnn'nnGTC
Cuts at: 1613 3024 1613
Size: 1411 5850
PsiI TTA'TAA
Cuts at: 2538 2991 4094 4831 2538
Size: 453 1103 737 4968
Fragments arranged by size:
4968 1103 737 453
PspOMI G'GGCC_C
Cuts at: 2616 2616
Size: 7261
PstI C_TGCA'G
Cuts at: 830 4613 830
Size: 3783 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7261
PvuII CAG'CTG
Cuts at: 2566 3080 4232 2566
Size: 514 1152 5595
Fragments arranged by size:
5595 1152 514
SalI G'TCGA_C
Cuts at: 1936 4603 1936
Size: 2667 4594
SapI GCTCTTCn'nnn_
Cuts at: 3072 4170 3072
Size: 1098 6163
SbfI CC_TGCA'GG
Cuts at: 4613 4613
Size: 7261
ScaI AGT'ACT
Cuts at: 5509 7221 5509
Size: 1712 5549
SfcI C'TryA_G
Cuts at: 826 1045 2314 4609 5744 6425 6616 826
Size: 219 1269 2295 1135 681 191 1471
Fragments arranged by size:
2295 1471 1269 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7261
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7261
SmaI CCC'GGG
Cuts at: 1872 4588 1872
Size: 2716 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2395 3022 5365 6266 6528 820
Size: 197 926 452 627 2343 901 262 1553
Fragments arranged by size:
2343 1553 926 901 627 452 262 197
SnaBI TAC'GTA
Cuts at: 493 5052 493
Size: 4559 2702
SpeI A'CTAG_T
Cuts at: 152 4504 152
Size: 4352 2909
SphI G_CATG'C
Cuts at: 1132 4619 1132
Size: 3487 3774
SspI AAT'ATT
Cuts at: 5 52 2113 2281 3719 5185 5
Size: 47 2061 168 1438 1466 2081
Fragments arranged by size:
2081 2061 1466 1438 168 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7261
StyI C'CwwG_G
Cuts at: 513 1065 2716 3969 4700 513
Size: 552 1651 1253 731 3074
Fragments arranged by size:
3074 1651 1253 731 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2022 5288 5305 5458 5643 6088 325
Size: 1255 442 3266 17 153 185 445 1498
Fragments arranged by size:
3266 1498 1255 445 442 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2677 3829 5507
Size: 274 80 33 51 1443 700 1152 1678
Cuts at: 5507 7219 96
Size: 1712 138
Fragments arranged by size:
1712 1678 1443 1152 700 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4188 4220 7221 7253 4188
Size: 32 3001 32 4196
Fragments arranged by size:
4196 3001 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7261
XbaI T'CTAG_A
Cuts at: 4597 4597
Size: 7261
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7261
XmaI C'CCGG_G
Cuts at: 1870 4586 1870
Size: 2716 4545
XmnI GAAnn'nnTTC
Cuts at: 3399 5390 3399
Size: 1991 5270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5069 276
Size: 53 83 186 936 3535 2468
Fragments arranged by size:
3535 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI