Restriction map for
pFN21AE2439
(Circular) (Six-base) MAPSORT of: pFN21AE2439.seq Check: 7392 from: 1 to: 5134
pFN21AE2439 5134 bp
With 182 enzymes: SgfI *
January 7, 2013 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 2192
Size: 5134
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2944 278
Size: 53 83 186 936 1408 2468
Fragments arranged by size:
2468 1408 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2477 1937
Size: 540 4594
Acc65I G'GTAC_C
Cuts at: 2455 2455
Size: 5134
AclI AA'CG_TT
Cuts at: 1563 3261 3634 1563
Size: 1698 373 3063
Fragments arranged by size:
3063 1698 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 3197 4581 4849 1632
Size: 713 852 1384 268 1917
Fragments arranged by size:
1917 1384 852 713 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4937
AflIII A'CryG_T
Cuts at: 1203 4033 5010 1203
Size: 2830 977 1327
Fragments arranged by size:
2830 1327 977
AgeI A'CCGG_T
Cuts at: 3965 3965
Size: 5134
AhdI GACnn_n'nnGTC
Cuts at: 3863 3863
Size: 5134
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5134
AlwNI CAG_nnn'CTG
Cuts at: 2877 4449 2877
Size: 1572 3562
ApaI G_GGCC'C
Cuts at: 2136 2194 2136
Size: 58 5076
ApaLI G'TGCA_C
Cuts at: 3191 4347 3191
Size: 1156 3978
ApoI r'AATT_y
Cuts at: 1493 2443 2669 2821 4930 1493
Size: 950 226 152 2109 1697
Fragments arranged by size:
2109 1697 950 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3688 160
Size: 872 2656 1606
Fragments arranged by size:
2656 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5134
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 2459 1870
Size: 73 158 358 4545
Fragments arranged by size:
4545 358 158 73
BamHI G'GATC_C
Cuts at: 2464 2831 2464
Size: 367 4767
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 2218 2300 2318 2455
Size: 325 217 627 393 38 82 18 137
Cuts at: 2455 3910 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 393 325 217 137 82
38 18
BanII G_rGCy'C
Cuts at: 1391 2136 2194 2453 1391
Size: 745 58 259 4072
Fragments arranged by size:
4072 745 259 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3325 3359 1903
Size: 34 50 34 1304 34 3678
Fragments arranged by size:
3678 1304 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3026 4236 3026
Size: 1210 3924
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5134
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2048 3745 136
Size: 107 122 71 631 605 376 1697 1525
Fragments arranged by size:
1697 1525 631 605 376 122 107 71
BglII A'GATC_T
Cuts at: 5129 5129
Size: 5134
BlpI GC'TnA_GC
Cuts at: 2551 2551
Size: 5134
Bme1580I G_kGCm'C
Cuts at: 1790 2136 2183 2194 2223 2297 3195 4351 1790
Size: 346 47 11 29 74 898 1156 2573
Fragments arranged by size:
2573 1156 898 346 74 47 29 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5134
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 2865 3823
Size: 416 53 83 399 24 867 581 958
Cuts at: 3823 442
Size: 1753
Fragments arranged by size:
1753 958 867 581 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2039
Size: 5134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3794 1239
Size: 2555 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3259 4124 4422 3259
Size: 865 298 3971
Fragments arranged by size:
3971 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 3797 882
Size: 617 852 1446 2219
Fragments arranged by size:
2219 1446 852 617
BsaAI yAC'GTr
Cuts at: 493 1204 2925 493
Size: 711 1721 2702
Fragments arranged by size:
2702 1721 711
BsaBI GATnn'nnATC
Cuts at: 5128 5128
Size: 5134
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2941 3323 3958 275
Size: 53 83 186 936 1408 382 635 1451
Fragments arranged by size:
1451 1408 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2208 3567 3965 4239 4386 4797 2208
Size: 1359 398 274 147 411 2545
Fragments arranged by size:
2545 1359 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4909
Fragments arranged by size:
4909 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2018 2027 1186
Size: 829 3 9 4293
Fragments arranged by size:
4293 829 9 3
BseYI C'CCAG_C
Cuts at: 2144 2199 4337 4833 2144
Size: 55 2138 496 2445
Fragments arranged by size:
2445 2138 496 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2122 2233 2365 1163
Size: 573 386 111 132 3932
Fragments arranged by size:
3932 573 386 132 111
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 2584 3345 4373 1540
Size: 275 186 66 517 761 1028 2301
Fragments arranged by size:
2301 1028 761 517 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 3195 3280 4351 3195
Size: 85 1071 3978
Fragments arranged by size:
3978 1071 85
BsmI GAATG_Cn'
Cuts at: 2645 2738 2645
Size: 93 5041
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2183 2194 2223 2297 2453 3195
Size: 399 346 47 11 29 74 156 742
Cuts at: 3195 3280 4351 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 156 85 74
47 29 11
BspEI T'CCGG_A
Cuts at: 4797 4797
Size: 5134
BspHI T'CATG_A
Cuts at: 3023 4753 3023
Size: 1730 3404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 2489 844
Size: 1348 297 3489
Fragments arranged by size:
3489 1348 297
BsrBI CCG'CTC
Cuts at: 1394 1823 3021 1394
Size: 429 1198 3507
Fragments arranged by size:
3507 1198 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3629 3803 65
Size: 1183 2381 174 1396
Fragments arranged by size:
2381 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3778 3965 1700
Size: 110 1968 187 2869
Fragments arranged by size:
2869 1968 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5134
BssHII G'CGCG_C
Cuts at: 1925 2333 1925
Size: 408 4726
BssSI C'ACGA_G
Cuts at: 3194 4206 3194
Size: 1012 4122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 2211 794
Size: 1363 54 3717
Fragments arranged by size:
3717 1363 54
BstBI TT'CG_AA
Cuts at: 3938 3938
Size: 5134
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5134
BstYI r'GATC_y
Cuts at: 1972 2464 2831 3216 3233 4685 4771 4783 5129
Size: 492 367 385 17 1452 86 12 346
Cuts at: 5129 1972
Size: 1977
Fragments arranged by size:
1977 1452 492 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2061 513
Size: 552 996 3586
Fragments arranged by size:
3586 996 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4395
Fragments arranged by size:
4395 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 3444 3464 2650
Size: 794 20 4320
Fragments arranged by size:
4320 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5099
DraI TTT'AAA
Cuts at: 2438 2438
Size: 5134
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4141 809
Size: 507 2825 1802
Fragments arranged by size:
2825 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2581 3470 5099
Size: 54 1425 50 275 18 751 889 1629
Cuts at: 5099 8
Size: 43
Fragments arranged by size:
1629 1425 889 751 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2581 1537
Size: 275 769 4090
Fragments arranged by size:
4090 769 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3064 1353
Size: 1711 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2300 3721 4095 4241 387
Size: 1064 138 711 1421 374 146 1280
Fragments arranged by size:
1421 1280 1064 711 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 3197 3794 4581 4849 1239
Size: 393 713 852 597 787 268 1524
Fragments arranged by size:
1524 852 787 713 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2327 2327
Size: 5134
EcoO109I rG'GnC_Cy
Cuts at: 2132 2133 2190 2132
Size: 1 57 5076
Fragments arranged by size:
5076 57 1
EcoRI G'AATT_C
Cuts at: 2443 2821 2443
Size: 378 4756
FspI TGC'GCA
Cuts at: 3640 3640
Size: 5134
HaeII r_GCGC'y
Cuts at: 1678 2054 4281 1678
Size: 376 2227 2531
Fragments arranged by size:
2531 2227 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3775 3807 3849 3881 1373
Size: 32 135 32 2203 32 42 32 2626
Fragments arranged by size:
2626 2203 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2478 2724 669
Size: 988 281 540 246 3079
Fragments arranged by size:
3079 988 540 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5134
HpaI GTT'AAC
Cuts at: 2724 2724
Size: 5134
KpnI G_GTAC'C
Cuts at: 2459 2459
Size: 5134
MfeI C'AATT_G
Cuts at: 2733 2733
Size: 5134
MluI A'CGCG_T
Cuts at: 5010 5010
Size: 5134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4248 4432 1305
Size: 2943 184 2007
Fragments arranged by size:
2943 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3312
Fragments arranged by size:
3312 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 3451 3610 518
Size: 648 1926 359 159 2042
Fragments arranged by size:
2042 1926 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2550 3227 4375 4620 1606
Size: 457 487 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 487 457 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4582
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5134
NotI GC'GGCC_GC
Cuts at: 2581 2581
Size: 5134
NspI r_CATG'y
Cuts at: 1132 1229 2492 4037 1132
Size: 97 1263 1545 2229
Fragments arranged by size:
2229 1545 1263 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5134
PciI A'CATG_T
Cuts at: 4033 4033
Size: 5134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5134
PmeI GTTT'AAAC
Cuts at: 2438 2438
Size: 5134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 4716 4748 3203
Size: 32 1481 32 3589
Fragments arranged by size:
3589 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5134
PsiI TTA'TAA
Cuts at: 2704 2704
Size: 5134
PspOMI G'GGCC_C
Cuts at: 2132 2190 2132
Size: 58 5076
PstI C_TGCA'G
Cuts at: 830 2486 830
Size: 1656 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5134
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 5134
SalI G'TCGA_C
Cuts at: 1936 2476 1936
Size: 540 4594
SbfI CC_TGCA'GG
Cuts at: 2486 2486
Size: 5134
ScaI AGT'ACT
Cuts at: 3382 5094 3382
Size: 1712 3422
SfcI C'TryA_G
Cuts at: 826 1045 2482 3617 4298 4489 826
Size: 219 1437 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5134
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5134
SmaI CCC'GGG
Cuts at: 1872 2103 2461 1872
Size: 231 358 4545
Fragments arranged by size:
4545 358 231
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3238 4139 4401 820
Size: 197 926 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2925 493
Size: 2432 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5134
SphI G_CATG'C
Cuts at: 1132 2492 1132
Size: 1360 3774
SspI AAT'ATT
Cuts at: 5 52 3058 5
Size: 47 3006 2081
Fragments arranged by size:
3006 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5134
StyI C'CwwG_G
Cuts at: 513 1065 2314 2573 513
Size: 552 1249 259 3074
Fragments arranged by size:
3074 1249 552 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3161 3178 3331 3516 3961 325
Size: 1255 1581 17 153 185 445 1498
Fragments arranged by size:
1581 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 3380 5092
Size: 274 80 33 51 1443 282 1121 1712
Cuts at: 5092 96
Size: 138
Fragments arranged by size:
1712 1443 1121 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5094 5126 5094
Size: 32 5102
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5134
XbaI T'CTAG_A
Cuts at: 2470 2470
Size: 5134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5134
XmaI C'CCGG_G
Cuts at: 1870 2101 2459 1870
Size: 231 358 4545
Fragments arranged by size:
4545 358 231
XmnI GAAnn'nnTTC
Cuts at: 3263 3263
Size: 5134
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2942 276
Size: 53 83 186 936 1408 2468
Fragments arranged by size:
2468 1408 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI