Restriction map for
pFN21AE2437
(Circular) (Six-base) MAPSORT of: pFN21AE2437.seq Check: 1007 from: 1 to: 6070
pFN21AE2437 6070 bp
With 182 enzymes: SgfI *
December 4, 2012 13:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2821 2821
Size: 6070
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2401 3413 1937
Size: 464 1012 4594
Fragments arranged by size:
4594 1012 464
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2027 2188 2478 4133 5517 5785 1632
Size: 395 161 290 1655 1384 268 1917
Fragments arranged by size:
1917 1655 1384 395 290 268 161
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 4969 5946 1203
Size: 3766 977 1327
Fragments arranged by size:
3766 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 2456 3813 5385 2456
Size: 1357 1572 3141
Fragments arranged by size:
3141 1572 1357
ApaI G_GGCC'C
Cuts at: 2439 2753 2799 2937 2439
Size: 314 46 138 5572
Fragments arranged by size:
5572 314 138 46
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 2034 3379 3605 3757 5866 1493
Size: 541 1345 226 152 2109 1697
Fragments arranged by size:
2109 1697 1345 541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2858 3395 1870
Size: 73 915 537 4545
Fragments arranged by size:
4545 915 537 73
BamHI G'GATC_C
Cuts at: 2543 2561 3400 3767 2543
Size: 18 839 367 4846
Fragments arranged by size:
4846 839 367 18
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3122 3391 4846 618
Size: 325 217 627 1335 269 1455 1842
Fragments arranged by size:
1842 1455 1335 627 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2439 2528 2753 2799 2937 3141 3389 1391
Size: 1048 89 225 46 138 204 248 4072
Fragments arranged by size:
4072 1048 248 225 204 138 89 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2704 928
Size: 971 805 4294
Fragments arranged by size:
4294 971 805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4261 4295 1903
Size: 34 2324 34 3678
Fragments arranged by size:
3678 2324 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2757 4681 136
Size: 107 122 71 631 605 1085 1924 1525
Fragments arranged by size:
1924 1525 1085 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3035 6065 3035
Size: 3030 3040
BlpI GC'TnA_GC
Cuts at: 3487 3487
Size: 6070
Bme1580I G_kGCm'C
Cuts at: 1790 2439 2753 2799 2937 4131 5287 1790
Size: 649 314 46 138 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 649 314 138 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2931 3322 3801
Size: 416 53 83 399 24 1514 391 479
Cuts at: 3801 4759 442
Size: 958 1753
Fragments arranged by size:
1753 1514 958 479 416 399 391 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2850 4730 1239
Size: 1043 568 1880 2579
Fragments arranged by size:
2579 1880 1043 568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3008 4195 5060 5358 3008
Size: 1187 865 298 3720
Fragments arranged by size:
3720 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 3021 3281 3306 4733 882
Size: 617 759 763 260 25 1427 2219
Fragments arranged by size:
2219 1427 763 759 617 260 25
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 3877 4259 4894
Size: 53 83 186 936 635 1709 382 635
Cuts at: 4894 275
Size: 1451
Fragments arranged by size:
1709 1451 936 635 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4503 4901 5175 5322 5733 4503
Size: 398 274 147 411 4840
Fragments arranged by size:
4840 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2158 2188 3329 3359 1178
Size: 30 165 30 755 30 1141 30 3889
Fragments arranged by size:
3889 1141 755 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2163 2996 3170 1186
Size: 977 833 174 4086
Fragments arranged by size:
4086 977 833 174
BseYI C'CCAG_C
Cuts at: 2117 5273 5769 2117
Size: 3156 496 2418
Fragments arranged by size:
3156 2418 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4131 4216 5287 4131
Size: 85 1071 4914
Fragments arranged by size:
4914 1071 85
BsmI GAATG_Cn'
Cuts at: 3581 3674 3581
Size: 93 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2439 2528 2753 2799 2937 3141 3389
Size: 399 649 89 225 46 138 204 248
Cuts at: 3389 4131 4216 5287 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 399 248 225 204
138 89 85 46
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2821 3324 3425 844
Size: 1977 503 101 3489
Fragments arranged by size:
3489 1977 503 101
BsrBI CCG'CTC
Cuts at: 1394 1823 3957 1394
Size: 429 2134 3507
Fragments arranged by size:
3507 2134 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3012 3326 4565 4739 65
Size: 1183 1764 314 1239 174 1396
Fragments arranged by size:
1764 1396 1239 1183 314 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3231 4714 4901 1700
Size: 110 1421 1483 187 2869
Fragments arranged by size:
2869 1483 1421 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 4130 5142 4130
Size: 1012 5058
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6070
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 2480 2579 3227 1184
Size: 1296 99 648 4027
Fragments arranged by size:
4027 1296 648 99
BstYI r'GATC_y
Cuts at: 1972 2543 2561 3035 3400 3767 4152 4169 5621
Size: 571 18 474 365 367 385 17 1452
Cuts at: 5621 5707 5719 6065 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 571 474 385 367 365 346
86 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4579
Fragments arranged by size:
4579 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3192 487
Size: 683 56 1966 3365
Fragments arranged by size:
3365 1966 683 56
BtsI GCAGTG_nn'
Cuts at: 3105 3586 4380 4400 3105
Size: 481 794 20 4775
Fragments arranged by size:
4775 794 481 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2272 5077 809
Size: 507 956 2805 1802
Fragments arranged by size:
2805 1802 956 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2212 2380
Size: 54 1425 50 275 18 313 69 168
Cuts at: 2380 2447 2785 3517 4406 6035 8
Size: 67 338 732 889 1629 43
Fragments arranged by size:
1629 1425 889 732 338 313 275 168
69 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4000 1353
Size: 2647 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 5031 5177 387
Size: 1064 138 3068 374 146 1280
Fragments arranged by size:
3068 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2188 2282 2478 2850 4133 4730
Size: 393 395 161 94 196 372 1283 597
Cuts at: 4730 5517 5785 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1283 787 597 395 393 372 268
196 161 94
EcoO109I rG'GnC_Cy
Cuts at: 2453 2462 2588 2687 2795 2796 2804 2834 2453
Size: 9 126 99 108 1 8 30 5689
Fragments arranged by size:
5689 126 108 99 30 9 8 1
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 5217 1678
Size: 3539 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 3020 3052 4711
Size: 32 135 32 586 32 830 32 1659
Cuts at: 4711 4743 4785 4817 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1659 830 586 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2780 2971 3414 3660 669
Size: 988 281 842 191 443 246 3079
Fragments arranged by size:
3079 988 842 443 281 246 191
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5184 5368 1305
Size: 3879 184 2007
Fragments arranged by size:
3879 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 2449 10
Size: 54 1425 343 313 304 3631
Fragments arranged by size:
3631 1425 343 313 304 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3234 3240 4028 4387 4546 518
Size: 648 2068 6 788 359 159 2042
Fragments arranged by size:
2068 2042 788 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 3079 3201 3486 4163 5311 5556 1606
Size: 1473 122 285 677 1148 245 2120
Fragments arranged by size:
2120 1473 1148 677 285 245 122
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4579
Fragments arranged by size:
4579 939 552
NdeI CA'TA_TG
Cuts at: 387 3307 387
Size: 2920 3150
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2157 2287 2431 3285 3428 4973 1132
Size: 97 928 130 144 854 143 1545 2229
Fragments arranged by size:
2229 1545 928 854 144 143 130 97
PasI CC'CwG_GG
Cuts at: 1348 2306 2323 2466 3299 1348
Size: 958 17 143 833 4119
Fragments arranged by size:
4119 958 833 143 17
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2445 1108
Size: 1337 4733
PfoI T'CCnGG_A
Cuts at: 2620 2866 2620
Size: 246 5824
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2462 2588 2687 2834 2462
Size: 126 99 147 5698
Fragments arranged by size:
5698 147 126 99
PshAI GACnn'nnGTC
Cuts at: 1613 2586 1613
Size: 973 5097
PsiI TTA'TAA
Cuts at: 2828 3640 2828
Size: 812 5258
PspOMI G'GGCC_C
Cuts at: 2435 2749 2795 2933 2435
Size: 314 46 138 5572
Fragments arranged by size:
5572 314 138 46
PstI C_TGCA'G
Cuts at: 830 2818 3112 3147 3422 830
Size: 1988 294 35 275 3478
Fragments arranged by size:
3478 1988 294 275 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 3079 3079
Size: 6070
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SanDI GG'GwC_CC
Cuts at: 2588 2834 2588
Size: 246 5824
SbfI CC_TGCA'GG
Cuts at: 2818 3422 2818
Size: 604 5466
ScaI AGT'ACT
Cuts at: 3332 4318 6030 3332
Size: 986 1712 3372
Fragments arranged by size:
3372 1712 986
SfcI C'TryA_G
Cuts at: 826 1045 2814 3108 3143 3418 4553 5234 5425
Size: 219 1769 294 35 275 1135 681 191
Cuts at: 5425 826
Size: 1471
Fragments arranged by size:
1769 1471 1135 681 294 275 219 191
35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2860 3397 1872
Size: 988 537 4545
Fragments arranged by size:
4545 988 537
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2987 4174 5075 5337 820
Size: 197 926 1044 1187 901 262 1553
Fragments arranged by size:
1553 1187 1044 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 2157 2287 2431 3428 1132
Size: 1025 130 144 997 3774
Fragments arranged by size:
3774 1025 997 144 130
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6070
StyI C'CwwG_G
Cuts at: 513 1065 2004 2529 3058 3509 513
Size: 552 939 525 529 451 3074
Fragments arranged by size:
3074 939 552 529 525 451
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 3173 3341 4097 4114 4267 4452
Size: 1255 1437 156 168 756 17 153 185
Cuts at: 4452 4897 325
Size: 445 1498
Fragments arranged by size:
1498 1437 1255 756 445 185 168 156
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3042 3330 4316
Size: 274 80 33 51 1443 1065 288 986
Cuts at: 4316 6028 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1065 986 288 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2172 2204 6030 6062 2172
Size: 32 3826 32 2180
Fragments arranged by size:
3826 2180 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6070
XmaI C'CCGG_G
Cuts at: 1870 2858 3395 1870
Size: 988 537 4545
Fragments arranged by size:
4545 988 537
XmnI GAAnn'nnTTC
Cuts at: 4199 4199
Size: 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI XcmI