Restriction map for
pFN21AE2433
(Circular) (Six-base) MAPSORT of: pFN21AE2433.seq Check: 5974 from: 1 to: 8617
pFN21AE2433 8617 bp
With 182 enzymes: SgfI *
February 22, 2013 16:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6427 278
Size: 53 83 186 936 4891 2468
Fragments arranged by size:
4891 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5960 1937
Size: 4023 4594
Acc65I G'GTAC_C
Cuts at: 5938 5938
Size: 8617
AclI AA'CG_TT
Cuts at: 1563 6744 7117 1563
Size: 5181 373 3063
Fragments arranged by size:
5181 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3137 3369 4164 4391 4986 5015 6680 8064
Size: 1505 232 795 227 595 29 1665 1384
Cuts at: 8064 8332 1632
Size: 268 1917
Fragments arranged by size:
1917 1665 1505 1384 795 595 268 232
227 29
AflII C'TTAA_G
Cuts at: 820 1017 2692 820
Size: 197 1675 6745
Fragments arranged by size:
6745 1675 197
AflIII A'CryG_T
Cuts at: 1203 5369 5586 7516 8493 1203
Size: 4166 217 1930 977 1327
Fragments arranged by size:
4166 1930 1327 977 217
AgeI A'CCGG_T
Cuts at: 7448 7448
Size: 8617
AhdI GACnn_n'nnGTC
Cuts at: 2712 7346 2712
Size: 4634 3983
AleI CACnn'nnGTG
Cuts at: 1166 4926 1166
Size: 3760 4857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8617
AlwNI CAG_nnn'CTG
Cuts at: 5764 6360 7932 5764
Size: 596 1572 6449
Fragments arranged by size:
6449 1572 596
ApaLI G'TGCA_C
Cuts at: 6674 7830 6674
Size: 1156 7461
ApoI r'AATT_y
Cuts at: 1493 2055 2130 3578 4331 5805 5926 6152 6304
Size: 562 75 1448 753 1474 121 226 152
Cuts at: 6304 8413 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1474 1448 753 562 226 152
121 75
AseI AT'TA_AT
Cuts at: 160 1032 7171 160
Size: 872 6139 1606
Fragments arranged by size:
6139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8617
AvaI C'yCGr_G
Cuts at: 1870 1943 5942 1870
Size: 73 3999 4545
Fragments arranged by size:
4545 3999 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4500 4533 4500
Size: 33 8584
BamHI G'GATC_C
Cuts at: 2767 5947 6314 2767
Size: 3180 367 5070
Fragments arranged by size:
5070 3180 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 5938 7393 618
Size: 325 217 627 843 3308 1455 1842
Fragments arranged by size:
3308 1842 1455 843 627 325 217
BanII G_rGCy'C
Cuts at: 1391 5199 5704 5936 1391
Size: 3808 505 232 4072
Fragments arranged by size:
4072 3808 505 232
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2432 4898 928
Size: 971 533 2466 4647
Fragments arranged by size:
4647 2466 971 533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4088 4122 6808 6842 1903
Size: 34 2151 34 2686 34 3678
Fragments arranged by size:
3678 2686 2151 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2156 3672 4217 6509 7719 2156
Size: 1516 545 2292 1210 3054
Fragments arranged by size:
3054 2292 1516 1210 545
BclI T'GATC_A
Cuts at: 1548 2617 4657 1548
Size: 1069 2040 5508
Fragments arranged by size:
5508 2040 1069
BfrBI ATG'CAT
Cuts at: 5023 5023
Size: 8617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2267 2850 7228
Size: 107 122 71 631 605 595 583 4378
Cuts at: 7228 136
Size: 1525
Fragments arranged by size:
4378 1525 631 605 595 583 122 107
71
BglII A'GATC_T
Cuts at: 4797 5047 8612 4797
Size: 250 3565 4802
Fragments arranged by size:
4802 3565 250
BlpI GC'TnA_GC
Cuts at: 2319 6034 2319
Size: 3715 4902
Bme1580I G_kGCm'C
Cuts at: 1790 6678 7834 1790
Size: 4888 1156 2573
Fragments arranged by size:
4888 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6348 7306 442
Size: 416 53 83 399 24 4931 958 1753
Fragments arranged by size:
4931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2522 1056
Size: 1466 7151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2117 2928 3156 2117
Size: 811 228 7578
Fragments arranged by size:
7578 811 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 2760 3012 3039 3102 3240 3267 7277
Size: 1320 201 252 27 63 138 27 4010
Cuts at: 7277 1239
Size: 2579
Fragments arranged by size:
4010 2579 1320 252 201 138 63 27
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2073 3656 6742 7607 7905 2073
Size: 1583 3086 865 298 2785
Fragments arranged by size:
3086 2785 1583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2663 3838 5538 7280 882
Size: 617 1164 1175 1700 1742 2219
Fragments arranged by size:
2219 1742 1700 1175 1164 617
BsaAI yAC'GTr
Cuts at: 493 1204 2167 6408 493
Size: 711 963 4241 2702
Fragments arranged by size:
4241 2702 963 711
BsaBI GATnn'nnATC
Cuts at: 4316 4656 8611 4316
Size: 340 3955 4322
Fragments arranged by size:
4322 3955 340
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6424 6806 7441 275
Size: 53 83 186 936 4891 382 635 1451
Fragments arranged by size:
4891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7050 7448 7722 7869 8280 7050
Size: 398 274 147 411 7387
Fragments arranged by size:
7387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2971 3001 3100 3130 3289
Size: 30 165 30 1568 30 99 30 159
Cuts at: 3289 3319 1178
Size: 30 6476
Fragments arranged by size:
6476 1568 165 159 99 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2934 3243 5664 1186
Size: 1748 309 2421 4139
Fragments arranged by size:
4139 2421 1748 309
BseYI C'CCAG_C
Cuts at: 2574 5773 7820 8316 2574
Size: 3199 2047 496 2875
Fragments arranged by size:
3199 2875 2047 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4560 5592 1163
Size: 573 2824 1032 4188
Fragments arranged by size:
4188 2824 1032 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6067 6828 7856 1540
Size: 275 186 4066 761 1028 2301
Fragments arranged by size:
4066 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 2869 4993 5199 6678 6763 7834 2325
Size: 544 2124 206 1479 85 1071 3108
Fragments arranged by size:
3108 2124 1479 1071 544 206 85
BsmI GAATG_Cn'
Cuts at: 2452 3924 4980 6128 6221 2452
Size: 1472 1056 1148 93 4848
Fragments arranged by size:
4848 1472 1148 1056 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2325 2869 4993 5199 5704 5936 6678
Size: 399 535 544 2124 206 505 232 742
Cuts at: 6678 6763 7834 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2124 1071 742 544 535 505 399
232 206 85
BspEI T'CCGG_A
Cuts at: 8280 8280
Size: 8617
BspHI T'CATG_A
Cuts at: 4887 5275 6506 8236 4887
Size: 388 1231 1730 5268
Fragments arranged by size:
5268 1730 1231 388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5972 844
Size: 5128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6504 1394
Size: 429 4681 3507
Fragments arranged by size:
4681 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2270 3548 4128 7112 7286 65
Size: 1183 1022 1278 580 2984 174 1396
Fragments arranged by size:
2984 1396 1278 1183 1022 580 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7261 7448 1700
Size: 110 5451 187 2869
Fragments arranged by size:
5451 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3684 4006 5551 96
Size: 3588 322 1545 3162
Fragments arranged by size:
3588 3162 1545 322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8617
BssSI C'ACGA_G
Cuts at: 3573 3728 6677 7689 3573
Size: 155 2949 1012 4501
Fragments arranged by size:
4501 2949 1012 155
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3812 4286 794
Size: 3018 474 5125
Fragments arranged by size:
5125 3018 474
BstBI TT'CG_AA
Cuts at: 3277 7421 3277
Size: 4144 4473
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8617
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3386 4564 3103
Size: 283 1178 7156
Fragments arranged by size:
7156 1178 283
BstYI r'GATC_y
Cuts at: 1972 2767 3723 4797 5047 5947 6314 6699 6716
Size: 795 956 1074 250 900 367 385 17
Cuts at: 6716 8168 8254 8266 8612 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1074 956 900 795 385 367
346 250 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2845 513
Size: 552 939 841 6285
Fragments arranged by size:
6285 939 841 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7878
Fragments arranged by size:
7878 683 56
BtsI GCAGTG_nn'
Cuts at: 5580 6133 6927 6947 5580
Size: 553 794 20 7250
Fragments arranged by size:
7250 794 553 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8582
DraI TTT'AAA
Cuts at: 3405 3510 4450 4824 5921 3405
Size: 105 940 374 1097 6101
Fragments arranged by size:
6101 1097 940 374 105
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7624 809
Size: 507 6308 1802
Fragments arranged by size:
6308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4125 6064 6953
Size: 54 1425 50 275 18 2295 1939 889
Cuts at: 6953 8582 8
Size: 1629 43
Fragments arranged by size:
2295 1939 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6064 1537
Size: 275 4252 4090
Fragments arranged by size:
4252 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3468 4950 6547 1353
Size: 2115 1482 1597 3423
Fragments arranged by size:
3423 2115 1597 1482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2389 4199 7204 7578 7724 387
Size: 1064 138 800 1810 3005 374 146 1280
Fragments arranged by size:
3005 1810 1280 1064 800 374 146 138
EcoICRI GAG'CTC
Cuts at: 5197 5197
Size: 8617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 2760 3012 3039 3102 3137 3240
Size: 393 927 201 252 27 63 35 103
Cuts at: 3240 3267 3369 4164 4391 4986 5015 6680 7277
Size: 27 102 795 227 595 29 1665 597
Cuts at: 7277 8064 8332 1239
Size: 787 268 1524
Fragments arranged by size:
1665 1524 927 795 787 597 595 393
268 252 227 201 103 102 63 35
29 27 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2811 3117 2811
Size: 306 8311
EcoO109I rG'GnC_Cy
Cuts at: 5710 5710
Size: 8617
EcoRI G'AATT_C
Cuts at: 4331 5926 6304 4331
Size: 1595 378 6644
Fragments arranged by size:
6644 1595 378
EcoRV GAT'ATC
Cuts at: 2255 2255
Size: 8617
FspI TGC'GCA
Cuts at: 7123 7123
Size: 8617
HaeII r_GCGC'y
Cuts at: 1678 7764 1678
Size: 6086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2201 2233 2419 2451 2698
Size: 32 135 32 629 32 186 32 247
Cuts at: 2698 2730 3289 3321 3368 3400 3598 3630 4423
Size: 32 559 32 47 32 198 32 793
Cuts at: 4423 4455 5220 5252 5659 5691 7258 7290 7332
Size: 32 765 32 407 32 1567 32 42
Cuts at: 7332 7364 1373
Size: 32 2626
Fragments arranged by size:
2626 1567 793 765 629 559 407 247
198 186 135 47 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2295 5239 5961 6207 669
Size: 988 281 357 2944 722 246 3079
Fragments arranged by size:
3079 2944 988 722 357 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8617
HpaI GTT'AAC
Cuts at: 6207 6207
Size: 8617
KpnI G_GTAC'C
Cuts at: 5942 5942
Size: 8617
MfeI C'AATT_G
Cuts at: 2175 3081 4617 6216 2175
Size: 906 1536 1599 4576
Fragments arranged by size:
4576 1599 1536 906
MluI A'CGCG_T
Cuts at: 8493 8493
Size: 8617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3332 7731 7915 1305
Size: 2027 4399 184 2007
Fragments arranged by size:
4399 2027 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4127 10
Size: 54 1425 343 2295 4500
Fragments arranged by size:
4500 2295 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2925 3101 4926 5018 6575 6934 7093
Size: 648 1759 176 1825 92 1557 359 159
Cuts at: 7093 518
Size: 2042
Fragments arranged by size:
2042 1825 1759 1557 648 359 176 159
92
MspA1I CmG'CkG
Cuts at: 1606 2615 2792 3260 4859 6033 6710 7858 8103
Size: 1009 177 468 1599 1174 677 1148 245
Cuts at: 8103 1606
Size: 2120
Fragments arranged by size:
2120 1599 1174 1148 1009 677 468 245
177
NcoI C'CATG_G
Cuts at: 513 1065 2004 2845 513
Size: 552 939 841 6285
Fragments arranged by size:
6285 939 841 552
NdeI CA'TA_TG
Cuts at: 387 3712 387
Size: 3325 5292
NheI G'CTAG_C
Cuts at: 1052 2518 1052
Size: 1466 7151
NotI GC'GGCC_GC
Cuts at: 6064 6064
Size: 8617
NsiI A_TGCA'T
Cuts at: 5025 5025
Size: 8617
NspI r_CATG'y
Cuts at: 1132 1229 3343 5373 5590 5975 7520 1132
Size: 97 2114 2030 217 385 1545 2229
Fragments arranged by size:
2229 2114 2030 1545 385 217 97
PasI CC'CwG_GG
Cuts at: 1348 2675 3919 1348
Size: 1327 1244 6046
Fragments arranged by size:
6046 1327 1244
PciI A'CATG_T
Cuts at: 5369 5586 7516 5369
Size: 217 1930 6470
Fragments arranged by size:
6470 1930 217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2846 1108
Size: 1738 6879
PfoI T'CCnGG_A
Cuts at: 2737 2806 2989 4556 2737
Size: 69 183 1567 6798
Fragments arranged by size:
6798 1567 183 69
PmeI GTTT'AAAC
Cuts at: 5921 5921
Size: 8617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3271 3303 6686 6718 8199 8231 3271
Size: 32 3383 32 1481 32 3657
Fragments arranged by size:
3657 3383 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8617
PsiI TTA'TAA
Cuts at: 6187 6187
Size: 8617
PstI C_TGCA'G
Cuts at: 830 5969 830
Size: 5139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8617
PvuII CAG'CTG
Cuts at: 2615 3260 4859 2615
Size: 645 1599 6373
Fragments arranged by size:
6373 1599 645
SacI G_AGCT'C
Cuts at: 5199 5199
Size: 8617
SalI G'TCGA_C
Cuts at: 1936 5959 1936
Size: 4023 4594
SapI GCTCTTCn'nnn_
Cuts at: 3468 3468
Size: 8617
SbfI CC_TGCA'GG
Cuts at: 5969 5969
Size: 8617
ScaI AGT'ACT
Cuts at: 5388 6865 8577 5388
Size: 1477 1712 5428
Fragments arranged by size:
5428 1712 1477
SexAI A'CCwGG_T
Cuts at: 2710 2710
Size: 8617
SfcI C'TryA_G
Cuts at: 826 1045 2109 4716 5343 5965 7100 7781 7972
Size: 219 1064 2607 627 622 1135 681 191
Cuts at: 7972 826
Size: 1471
Fragments arranged by size:
2607 1471 1135 1064 681 627 622 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8617
SmaI CCC'GGG
Cuts at: 1872 5944 1872
Size: 4072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2088 2692 3635 6721 7622 7884
Size: 197 926 145 604 943 3086 901 262
Cuts at: 7884 820
Size: 1553
Fragments arranged by size:
3086 1553 943 926 901 604 262 197
145
SnaBI TAC'GTA
Cuts at: 493 6408 493
Size: 5915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8617
SphI G_CATG'C
Cuts at: 1132 5975 1132
Size: 4843 3774
SspI AAT'ATT
Cuts at: 5 52 5750 6541 5
Size: 47 5698 791 2081
Fragments arranged by size:
5698 2081 791 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2845 4137 6056 513
Size: 552 939 841 1292 1919 3074
Fragments arranged by size:
3074 1919 1292 939 841 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3032 3308 4916 4940 5787 6644 6661
Size: 1255 1452 276 1608 24 847 857 17
Cuts at: 6661 6814 6999 7444 325
Size: 153 185 445 1498
Fragments arranged by size:
1608 1498 1452 1255 857 847 445 276
185 153 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3684 4006 4593
Size: 274 80 33 51 1443 1707 322 587
Cuts at: 4593 5095 5386 5551 6863 8575 96
Size: 502 291 165 1312 1712 138
Fragments arranged by size:
1712 1707 1443 1312 587 502 322 291
274 165 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4102 4134 4387 4419 8577 8609 4102
Size: 32 253 32 4158 32 4110
Fragments arranged by size:
4158 4110 253 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8617
XbaI T'CTAG_A
Cuts at: 5953 5953
Size: 8617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 4120 4135 2132
Size: 1988 15 6614
Fragments arranged by size:
6614 1988 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8617
XmaI C'CCGG_G
Cuts at: 1870 5942 1870
Size: 4072 4545
XmnI GAAnn'nnTTC
Cuts at: 3150 6746 3150
Size: 3596 5021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6425 276
Size: 53 83 186 936 4891 2468
Fragments arranged by size:
4891 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI