Restriction map for
pFN21AE2425
(Circular) (Six-base) MAPSORT of: pFN21AE2425.seq Check: 9597 from: 1 to: 5692
pFN21AE2425 5692 bp
With 182 enzymes: SgfI *
November 22, 2012 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2609 2609
Size: 5692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2308 2462 3755 5139 5407 1632
Size: 676 154 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 676 268 154
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 2254 1166
Size: 1088 4604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2321 1184
Size: 1137 4555
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2522 2554 2522
Size: 32 5660
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaI G_GGCC'C
Cuts at: 2096 2393 2473 2588 2096
Size: 297 80 115 5200
Fragments arranged by size:
5200 297 115 80
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2096 3017 1870
Size: 73 123 30 921 4545
Fragments arranged by size:
4545 921 123 73 30
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 3013 4468 618
Size: 325 217 627 939 287 1455 1842
Fragments arranged by size:
1842 1455 939 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 2096 2393 2473 2588 3011 1391
Size: 705 297 80 115 423 4072
Fragments arranged by size:
4072 705 423 297 115 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2713 928
Size: 971 814 3907
Fragments arranged by size:
3907 971 814
BbvCI CC'TCA_GC
Cuts at: 2132 2132
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2059 4303
Size: 107 122 71 631 605 337 50 2244
Cuts at: 4303 136
Size: 1525
Fragments arranged by size:
2244 1525 631 605 337 122 107 71
50
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2393 2473 2588 3753 4909 1790
Size: 306 297 80 115 1165 1156 2573
Fragments arranged by size:
2573 1165 1156 306 297 115 80
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2351 2885 4352 1239
Size: 1051 61 534 1467 2579
Fragments arranged by size:
2579 1467 1051 534 61
Bpu10I CC'TnA_GC
Cuts at: 2132 2132
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2152 3483 493
Size: 711 948 1331 2702
Fragments arranged by size:
2702 1331 948 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2522 2552 1178
Size: 30 165 30 1119 30 4318
Fragments arranged by size:
4318 1119 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 4335
BseYI C'CCAG_C
Cuts at: 2014 2080 2109 2344 2812 4895 5391 2014
Size: 66 29 235 468 2083 496 2315
Fragments arranged by size:
2315 2083 496 468 235 66 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2054 2063 3142 3903 4931 1540
Size: 275 186 53 9 1079 761 1028 2301
Fragments arranged by size:
2301 1079 1028 761 275 186 53 9
BsiHKAI G_wGCw'C
Cuts at: 2838 2990 3753 3838 4909 2838
Size: 152 763 85 1071 3621
Fragments arranged by size:
3621 1071 763 152 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2393 2473 2588 2838 2990 3011
Size: 399 306 297 80 115 250 152 21
Cuts at: 3011 3753 3838 4909 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 297 250 152
115 85 80 21
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2609 3047 844
Size: 1765 438 3489
Fragments arranged by size:
3489 1765 438
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2463 2608 2959 3579 1394
Size: 429 236 404 145 351 620 3507
Fragments arranged by size:
3507 620 429 404 351 236 145
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2773 4336 4523 1700
Size: 110 963 1563 187 2869
Fragments arranged by size:
2869 1563 963 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2867 794
Size: 2073 3619
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2230 2230
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2199 513
Size: 552 939 195 4006
Fragments arranged by size:
4006 939 552 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 3208 4002 4022 2733
Size: 475 794 20 4403
Fragments arranged by size:
4403 794 475 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2051 2060 3139
Size: 54 1425 50 275 18 221 9 1079
Cuts at: 3139 4028 5657 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1079 889 275 221 54 50
43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2051 2060 3139 1537
Size: 275 239 9 1079 4090
Fragments arranged by size:
4090 1079 275 239 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 2750 2891 3622 1353
Size: 815 582 141 731 3423
Fragments arranged by size:
3423 815 731 582 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2648 4279 4653 4799 387
Size: 1064 138 1059 1631 374 146 1280
Fragments arranged by size:
1631 1280 1064 1059 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2308 2351 2462 2885 3755 4352
Size: 393 658 18 43 111 423 870 597
Cuts at: 4352 5139 5407 1239
Size: 787 268 1524
Fragments arranged by size:
1524 870 787 658 597 423 393 268
111 43 18
EcoO109I rG'GnC_Cy
Cuts at: 2093 2585 2093
Size: 492 5200
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 5692
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2015 2081 2354 4839 1678
Size: 337 66 273 2485 2531
Fragments arranged by size:
2531 2485 337 273 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4333 4365 4407 4439 1373
Size: 32 135 32 2761 32 42 32 2626
Fragments arranged by size:
2761 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2254 2315 2486 3650 4009 4168 518
Size: 648 1088 61 171 1164 359 159 2042
Fragments arranged by size:
2042 1164 1088 648 359 171 159 61
MspA1I CmG'CkG
Cuts at: 1606 2201 2348 2475 2521 2650 2717 2816 3108
Size: 595 147 127 46 129 67 99 292
Cuts at: 3108 3785 4933 5178 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 292 245 147 129
127 99 67 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 2060 3139 2060
Size: 1079 4613
NspI r_CATG'y
Cuts at: 1132 1229 2491 3050 4595 1132
Size: 97 1262 559 1545 2229
Fragments arranged by size:
2229 1545 1262 559 97
PasI CC'CwG_GG
Cuts at: 1348 2187 2872 1348
Size: 839 685 4168
Fragments arranged by size:
4168 839 685
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2522 2554 3761 3793 5274 5306 2522
Size: 32 1207 32 1481 32 2908
Fragments arranged by size:
2908 1481 1207 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2038 1613
Size: 425 5267
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2092 2389 2469 2584 2092
Size: 297 80 115 5200
Fragments arranged by size:
5200 297 115 80
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2650 2717 2816 2650
Size: 67 99 5526
Fragments arranged by size:
5526 99 67
SacII CC_GC'GG
Cuts at: 2202 2202
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SapI GCTCTTCn'nnn_
Cuts at: 2168 2168
Size: 5692
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 3040 4175 4856 5047 826
Size: 219 1995 1135 681 191 1471
Fragments arranged by size:
1995 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2059 2059
Size: 5692
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 2098 3019 1872
Size: 226 921 4545
Fragments arranged by size:
4545 921 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2829 3131 513
Size: 552 939 825 302 3074
Fragments arranged by size:
3074 939 825 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 2853 2864 3719 3736 3889 4074
Size: 1255 521 752 11 855 17 153 185
Cuts at: 4074 4519 325
Size: 445 1498
Fragments arranged by size:
1498 1255 855 752 521 445 185 153
17 11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2230 2011
Size: 219 5473
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 2096 3017 1870
Size: 226 921 4545
Fragments arranged by size:
4545 921 226
XmnI GAAnn'nnTTC
Cuts at: 2681 3821 2681
Size: 1140 4552
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI
SexAI SfoI SrfI SwaI