Restriction map for
pFN21AE2424
(Circular) (Six-base) MAPSORT of: pFN21AE2424.seq Check: 6136 from: 1 to: 7192
pFN21AE2424 7192 bp
With 182 enzymes: SgfI *
November 9, 2012 16:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5002 278
Size: 53 83 186 936 3466 2468
Fragments arranged by size:
3466 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4535 1937
Size: 2598 4594
Acc65I G'GTAC_C
Cuts at: 4513 4513
Size: 7192
AclI AA'CG_TT
Cuts at: 1563 5319 5692 1563
Size: 3756 373 3063
Fragments arranged by size:
3756 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 3791 5255 6639 6907 1632
Size: 930 1229 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 1229 930 268
AfeI AGC'GCT
Cuts at: 3019 3142 3019
Size: 123 7069
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6995
AflIII A'CryG_T
Cuts at: 1203 6091 7068 1203
Size: 4888 977 1327
Fragments arranged by size:
4888 1327 977
AgeI A'CCGG_T
Cuts at: 2812 6023 2812
Size: 3211 3981
AhdI GACnn_n'nnGTC
Cuts at: 5921 5921
Size: 7192
AleI CACnn'nnGTG
Cuts at: 1166 2120 4262 1166
Size: 954 2142 4096
Fragments arranged by size:
4096 2142 954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4082 1184
Size: 2898 4294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2372
Size: 32 7160
AlwNI CAG_nnn'CTG
Cuts at: 4311 4935 6507 4311
Size: 624 1572 4996
Fragments arranged by size:
4996 1572 624
ApaI G_GGCC'C
Cuts at: 2079 3586 2079
Size: 1507 5685
ApaLI G'TGCA_C
Cuts at: 5249 6405 5249
Size: 1156 6036
ApoI r'AATT_y
Cuts at: 1493 4501 4727 4879 6988 1493
Size: 3008 226 152 2109 1697
Fragments arranged by size:
3008 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5746 160
Size: 872 4714 1606
Fragments arranged by size:
4714 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7192
AvaI C'yCGr_G
Cuts at: 1870 1943 3039 3186 3979 4517 1870
Size: 73 1096 147 793 538 4545
Fragments arranged by size:
4545 1096 793 538 147 73
BamHI G'GATC_C
Cuts at: 3621 4522 4889 3621
Size: 901 367 5924
Fragments arranged by size:
5924 901 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2287 2826 3789 4513
Size: 325 217 627 449 51 539 963 724
Cuts at: 4513 5968 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 963 724 627 539 449 325
217 51
BanII G_rGCy'C
Cuts at: 1391 2079 2538 2780 3206 3586 4511 1391
Size: 688 459 242 426 380 925 4072
Fragments arranged by size:
4072 925 688 459 426 380 242
BbeI G_GCGC'C
Cuts at: 2240 2240
Size: 7192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2759 2766 2984 3361 3895 4480 928
Size: 971 860 7 218 377 534 585 3640
Fragments arranged by size:
3640 971 860 585 534 377 218 7
BbvCI CC'TCA_GC
Cuts at: 3506 3938 3506
Size: 432 6760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4345 4379 5383 5417 1903
Size: 34 2408 34 1004 34 3678
Fragments arranged by size:
3678 2408 1004 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4453 5084 6294 4453
Size: 631 1210 5351
Fragments arranged by size:
5351 1210 631
BclI T'GATC_A
Cuts at: 1548 3399 1548
Size: 1851 5341
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3833 5803 136
Size: 107 122 71 631 605 2161 1970 1525
Fragments arranged by size:
2161 1970 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3336 4479 7187 3336
Size: 1143 2708 3341
Fragments arranged by size:
3341 2708 1143
BlpI GC'TnA_GC
Cuts at: 4609 4609
Size: 7192
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2292 2829 3586 3610 3659 3707 5253
Size: 289 213 537 757 24 49 48 1546
Cuts at: 5253 6409 1790
Size: 1156 2573
Fragments arranged by size:
2573 1546 1156 757 537 289 213 49
48 24
BmgBI CAC'GTC
Cuts at: 1318 2871 1318
Size: 1553 5639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2487 2512 3159
Size: 416 53 83 399 24 1070 25 647
Cuts at: 3159 3485 4923 5881 442
Size: 326 1438 958 1753
Fragments arranged by size:
1753 1438 1070 958 647 416 399 326
83 53 25 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2983 5852 1239
Size: 1744 2869 2579
Fragments arranged by size:
2869 2579 1744
Bpu10I CC'TnA_GC
Cuts at: 2471 3506 3938 2471
Size: 1035 432 5725
Fragments arranged by size:
5725 1035 432
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2844 5317 6182 6480 2844
Size: 2473 865 298 3556
Fragments arranged by size:
3556 2473 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5855 882
Size: 617 4356 2219
Fragments arranged by size:
4356 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 2398 4006 4051 4983 493
Size: 711 918 276 1608 45 932 2702
Fragments arranged by size:
2702 1608 932 918 711 276 45
BsaBI GATnn'nnATC
Cuts at: 7186 7186
Size: 7192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 2237 4999 5381
Size: 53 83 186 936 567 137 2762 382
Cuts at: 5381 6016 275
Size: 635 1451
Fragments arranged by size:
2762 1451 936 635 567 382 186 137
83 53
BsaWI w'CCGG_w
Cuts at: 2812 4209 5625 6023 6297 6444 6855 2812
Size: 1397 1416 398 274 147 411 3149
Fragments arranged by size:
3149 1416 1397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2402 3080 3110 4385
Size: 30 165 30 969 30 678 30 1275
Cuts at: 4385 4415 1178
Size: 30 3955
Fragments arranged by size:
3955 1275 969 678 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3227 3926 4352 4393 1186
Size: 2041 699 426 41 3985
Fragments arranged by size:
3985 2041 699 426 41
BseYI C'CCAG_C
Cuts at: 2720 2952 3529 3859 6395 6891 2720
Size: 232 577 330 2536 496 3021
Fragments arranged by size:
3021 2536 577 496 330 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3410 4054 4487 1163
Size: 573 1674 644 433 3868
Fragments arranged by size:
3868 1674 644 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 2817 4642 5403 6431 1540
Size: 275 186 51 765 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 765 761 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 2037 2472 2538 3206 4011 4344 5253 5338 6409
Size: 435 66 668 805 333 909 85 1071
Cuts at: 6409 2037
Size: 2820
Fragments arranged by size:
2820 1071 909 805 668 435 333 85
66
BsmI GAATG_Cn'
Cuts at: 4703 4796 4703
Size: 93 7099
BsmBI CGTCTCn'nnnn_
Cuts at: 2092 2092
Size: 7192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2079 2292 2472 2538 2780 2829
Size: 399 247 42 213 180 66 242 49
Cuts at: 2829 3206 3586 3610 3659 3707 4011 4344 4511
Size: 377 380 24 49 48 304 333 167
Cuts at: 4511 5253 5338 6409 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 377 333 304
247 242 213 180 167 85 66 49
49 48 42 24
BspEI T'CCGG_A
Cuts at: 4209 6855 4209
Size: 2646 4546
BspHI T'CATG_A
Cuts at: 4200 5081 6811 4200
Size: 881 1730 4581
Fragments arranged by size:
4581 1730 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2937 4179 4547 844
Size: 2093 1242 368 3489
Fragments arranged by size:
3489 2093 1242 368
BsrBI CCG'CTC
Cuts at: 1394 1823 4045 5079 1394
Size: 429 2222 1034 3507
Fragments arranged by size:
3507 2222 1034 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3351 3360 3601 5687 5861 65
Size: 1183 2103 9 241 2086 174 1396
Fragments arranged by size:
2103 2086 1396 1183 241 174 9
BsrFI r'CCGG_y
Cuts at: 1700 1810 2812 3963 5836 6023 1700
Size: 110 1002 1151 1873 187 2869
Fragments arranged by size:
2869 1873 1151 1002 187 110
BsrGI T'GTAC_A
Cuts at: 96 3744 96
Size: 3648 3544
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7192
BssSI C'ACGA_G
Cuts at: 5252 6264 5252
Size: 1012 6180
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4254 794
Size: 3460 3732
BstBI TT'CG_AA
Cuts at: 5996 5996
Size: 7192
BstEII G'GTnAC_C
Cuts at: 1184 2569 3032 3167 1184
Size: 1385 463 135 5209
Fragments arranged by size:
5209 1385 463 135
BstYI r'GATC_y
Cuts at: 1972 3116 3336 3621 3984 4479 4522 4889 5274
Size: 1144 220 285 363 495 43 367 385
Cuts at: 5274 5291 6743 6829 6841 7187 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1144 495 385 367 363 346
285 220 86 43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3651 4263 513
Size: 552 2586 612 3442
Fragments arranged by size:
3442 2586 612 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3907 4393 487
Size: 683 56 2681 486 3286
Fragments arranged by size:
3286 2681 683 486 56
BtsI GCAGTG_nn'
Cuts at: 4037 4708 5502 5522 4037
Size: 671 794 20 5707
Fragments arranged by size:
5707 794 671 20
ClaI AT'CG_AT
Cuts at: 1554 3243 1554
Size: 1689 5503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7157
DraI TTT'AAA
Cuts at: 4496 4496
Size: 7192
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3257 6199 809
Size: 507 1941 2942 1802
Fragments arranged by size:
2942 1941 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2691 2991 3834
Size: 54 1425 50 275 18 861 300 843
Cuts at: 3834 4152 4639 5528 7157 8
Size: 318 487 889 1629 43
Fragments arranged by size:
1629 1425 889 861 843 487 318 300
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4639 1537
Size: 275 2827 4090
Fragments arranged by size:
4090 2827 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4289 5122 1353
Size: 2936 833 3423
Fragments arranged by size:
3423 2936 833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2246 3980 5779 6153 6299 387
Size: 1064 138 657 1734 1799 374 146 1280
Fragments arranged by size:
1799 1734 1280 1064 657 374 146 138
EcoICRI GAG'CTC
Cuts at: 2536 3204 2536
Size: 668 6524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 2983 3791 5255 5852 6639 6907
Size: 393 930 421 808 1464 597 787 268
Cuts at: 6907 1239
Size: 1524
Fragments arranged by size:
1524 1464 930 808 787 597 421 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2075 3154 3583 4422 2075
Size: 1079 429 839 4845
Fragments arranged by size:
4845 1079 839 429
EcoRI G'AATT_C
Cuts at: 4501 4879 4501
Size: 378 6814
FspI TGC'GCA
Cuts at: 3593 5698 3593
Size: 2105 5087
HaeII r_GCGC'y
Cuts at: 1678 2240 3021 3144 3492 3813 6339 1678
Size: 562 781 123 348 321 2526 2531
Fragments arranged by size:
2531 2526 781 562 348 321 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5833 5865 5907 5939 1373
Size: 32 135 32 4261 32 42 32 2626
Fragments arranged by size:
4261 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 2149 2425 3136 4536 4782
Size: 988 281 196 15 276 711 1400 246
Cuts at: 4782 669
Size: 3079
Fragments arranged by size:
3079 1400 988 711 281 276 246 196
15
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7192
HpaI GTT'AAC
Cuts at: 4782 4782
Size: 7192
KasI G'GCGC_C
Cuts at: 2236 2236
Size: 7192
KpnI G_GTAC'C
Cuts at: 4517 4517
Size: 7192
MfeI C'AATT_G
Cuts at: 4791 4791
Size: 7192
MluI A'CGCG_T
Cuts at: 7068 7068
Size: 7192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2358 2601 3273 6306 6490 1305
Size: 1053 243 672 3033 184 2007
Fragments arranged by size:
3033 2007 1053 672 243 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2993 3836 4154 10
Size: 54 1425 343 1161 843 318 3048
Fragments arranged by size:
3048 1425 1161 843 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2663 3746 4199 4262 4412 5150
Size: 648 954 543 1083 453 63 150 738
Cuts at: 5150 5509 5668 518
Size: 359 159 2042
Fragments arranged by size:
2042 1083 954 738 648 543 453 359
159 150 63
MspA1I CmG'CkG
Cuts at: 1606 3394 4038 4471 4608 5285 6433 6678 1606
Size: 1788 644 433 137 677 1148 245 2120
Fragments arranged by size:
2120 1788 1148 677 644 433 245 137
NarI GG'CG_CC
Cuts at: 2237 2237
Size: 7192
NcoI C'CATG_G
Cuts at: 513 1065 3651 513
Size: 552 2586 4054
Fragments arranged by size:
4054 2586 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7192
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7192
NotI GC'GGCC_GC
Cuts at: 4639 4639
Size: 7192
NspI r_CATG'y
Cuts at: 1132 1229 2668 3769 4550 6095 1132
Size: 97 1439 1101 781 1545 2229
Fragments arranged by size:
2229 1545 1439 1101 781 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7192
PciI A'CATG_T
Cuts at: 6091 6091
Size: 7192
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 3415 3475 1108
Size: 1230 1077 60 4825
Fragments arranged by size:
4825 1230 1077 60
PmeI GTTT'AAAC
Cuts at: 4496 4496
Size: 7192
PmlI CAC'GTG
Cuts at: 4006 4006
Size: 7192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2372 2404 5261 5293 6774 6806 2372
Size: 32 2857 32 1481 32 2758
Fragments arranged by size:
2857 2758 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3154 4422 3154
Size: 1268 5924
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7192
PsiI TTA'TAA
Cuts at: 4762 4762
Size: 7192
PspOMI G'GGCC_C
Cuts at: 2075 3582 2075
Size: 1507 5685
PspXI vC'TCGA_Gb
Cuts at: 3039 3039
Size: 7192
PstI C_TGCA'G
Cuts at: 830 4330 4544 830
Size: 3500 214 3478
Fragments arranged by size:
3500 3478 214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7192
PvuII CAG'CTG
Cuts at: 3394 3394
Size: 7192
SacI G_AGCT'C
Cuts at: 2538 3206 2538
Size: 668 6524
SalI G'TCGA_C
Cuts at: 1936 4534 1936
Size: 2598 4594
SapI GCTCTTCn'nnn_
Cuts at: 4289 4289
Size: 7192
SbfI CC_TGCA'GG
Cuts at: 4544 4544
Size: 7192
ScaI AGT'ACT
Cuts at: 2522 5440 7152 2522
Size: 2918 1712 2562
Fragments arranged by size:
2918 2562 1712
SfcI C'TryA_G
Cuts at: 826 1045 3814 4326 4540 5675 6356 6547 826
Size: 219 2769 512 214 1135 681 191 1471
Fragments arranged by size:
2769 1471 1135 681 512 219 214 191
SfoI GGC'GCC
Cuts at: 2238 2238
Size: 7192
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7192
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7192
SmaI CCC'GGG
Cuts at: 1872 4519 1872
Size: 2647 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2859 3039 5296 6197 6459 820
Size: 197 926 916 180 2257 901 262 1553
Fragments arranged by size:
2257 1553 926 916 901 262 197 180
SnaBI TAC'GTA
Cuts at: 493 4983 493
Size: 4490 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7192
SphI G_CATG'C
Cuts at: 1132 3769 4550 1132
Size: 2637 781 3774
Fragments arranged by size:
3774 2637 781
SspI AAT'ATT
Cuts at: 5 52 5116 5
Size: 47 5064 2081
Fragments arranged by size:
5064 2081 47
StuI AGG'CCT
Cuts at: 1517 2585 1517
Size: 1068 6124
StyI C'CwwG_G
Cuts at: 513 1065 3028 3651 4631 513
Size: 552 1963 623 980 3074
Fragments arranged by size:
3074 1963 980 623 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2771 3015 3251 3486 5219 5236
Size: 1255 458 733 244 236 235 1733 17
Cuts at: 5236 5389 5574 6019 325
Size: 153 185 445 1498
Fragments arranged by size:
1733 1498 1255 733 458 445 244 236
235 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3744 5438
Size: 274 80 33 51 1443 543 1224 1694
Cuts at: 5438 7150 96
Size: 1712 138
Fragments arranged by size:
1712 1694 1443 1224 543 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2606 2638 4385 4417 7152 7184 2606
Size: 32 1747 32 2735 32 2614
Fragments arranged by size:
2735 2614 1747 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2129 1316
Size: 813 6379
XbaI T'CTAG_A
Cuts at: 4528 4528
Size: 7192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3205 2011
Size: 1194 5998
XhoI C'TCGA_G
Cuts at: 1943 3039 1943
Size: 1096 6096
XmaI C'CCGG_G
Cuts at: 1870 4517 1870
Size: 2647 4545
XmnI GAAnn'nnTTC
Cuts at: 5321 5321
Size: 7192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5000 276
Size: 53 83 186 936 3466 2468
Fragments arranged by size:
3466 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI