Restriction map for
pFN21AE2423
(Circular) (Six-base) MAPSORT of: pFN21AE2423.seq Check: 748 from: 1 to: 5539
pFN21AE2423 5539 bp
With 182 enzymes: SgfI *
October 29, 2012 18:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2745 2882 1937
Size: 808 137 4594
Fragments arranged by size:
4594 808 137
Acc65I G'GTAC_C
Cuts at: 2298 2860 2298
Size: 562 4977
AclI AA'CG_TT
Cuts at: 1563 2456 3666 4039 1563
Size: 893 1210 373 3063
Fragments arranged by size:
3063 1210 893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2790 3602 4986 5254 1632
Size: 893 265 812 1384 268 1917
Fragments arranged by size:
1917 1384 893 812 268 265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 2187 1166
Size: 1021 4518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2487 1184
Size: 1303 4236
AlwNI CAG_nnn'CTG
Cuts at: 3282 4854 3282
Size: 1572 3967
ApaLI G'TGCA_C
Cuts at: 3596 4752 3596
Size: 1156 4383
ApoI r'AATT_y
Cuts at: 1493 2156 2448 2594 2723 2848 3074 3226 5335
Size: 663 292 146 129 125 226 152 2109
Cuts at: 5335 1493
Size: 1697
Fragments arranged by size:
2109 1697 663 292 226 152 146 129
125
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2864 1870
Size: 73 921 4545
Fragments arranged by size:
4545 921 73
BamHI G'GATC_C
Cuts at: 2612 2869 3236 2612
Size: 257 367 4915
Fragments arranged by size:
4915 367 257
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2860 4315 618
Size: 325 217 627 511 562 1455 1842
Fragments arranged by size:
1842 1455 627 562 511 325 217
BanII G_rGCy'C
Cuts at: 1391 2858 1391
Size: 1467 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2320 2332 928
Size: 971 421 12 4135
Fragments arranged by size:
4135 971 421 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2123 3431 4641 2123
Size: 1308 1210 3021
Fragments arranged by size:
3021 1308 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5534 5534
Size: 5539
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 3600 4756 1790
Size: 1810 1156 2573
Fragments arranged by size:
2573 1810 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3270 4228 442
Size: 416 53 83 399 24 1853 958 1753
Fragments arranged by size:
1853 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4199 1239
Size: 2960 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4202 882
Size: 617 2703 2219
Fragments arranged by size:
2703 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3330 493
Size: 711 2126 2702
Fragments arranged by size:
2702 2126 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3346 3728 4363 275
Size: 53 83 186 936 1813 382 635 1451
Fragments arranged by size:
1813 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5314
Fragments arranged by size:
5314 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2645 1186
Size: 1122 337 4080
Fragments arranged by size:
4080 1122 337
BseYI C'CCAG_C
Cuts at: 4742 5238 4742
Size: 496 5043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4966
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3600 3685 4756 3600
Size: 85 1071 4383
Fragments arranged by size:
4383 1071 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2858 3600 3685 4756 1391
Size: 399 1068 742 85 1071 2174
Fragments arranged by size:
2174 1071 1068 742 399 85
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 844
Size: 2050 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4183 4370 1700
Size: 110 2373 187 2869
Fragments arranged by size:
2869 2373 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5539
BssSI C'ACGA_G
Cuts at: 3599 4611 3599
Size: 1012 4527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5539
BstBI TT'CG_AA
Cuts at: 2152 4343 2152
Size: 2191 3348
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2393 2612 2869 3236 3621 3638 5090 5176
Size: 421 219 257 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 421 385 367 346 257 219
86 17 12
BstZ17I GTA'TAC
Cuts at: 2746 2746
Size: 5539
BtgI C'CryG_G
Cuts at: 513 1065 2004 2318 513
Size: 552 939 314 3734
Fragments arranged by size:
3734 939 552 314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2101 2843 2101
Size: 742 4797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2086 2751 3469 1353
Size: 733 665 718 3423
Fragments arranged by size:
3423 733 718 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4126 4500 4646 387
Size: 1064 138 2537 374 146 1280
Fragments arranged by size:
2537 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 2790 3602 4199 4986 5254 1239
Size: 393 893 265 812 597 787 268 1524
Fragments arranged by size:
1524 893 812 787 597 393 268 265
EcoRI G'AATT_C
Cuts at: 2723 2848 3226 2723
Size: 125 378 5036
Fragments arranged by size:
5036 378 125
EcoRV GAT'ATC
Cuts at: 2686 2686
Size: 5539
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 4686 1678
Size: 3008 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 4180 4212 4254
Size: 32 135 32 1025 32 1551 32 42
Cuts at: 4254 4286 1373
Size: 32 2626
Fragments arranged by size:
2626 1551 1025 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 3129 669
Size: 988 281 699 246 246 3079
Fragments arranged by size:
3079 988 699 281 246 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5539
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KpnI G_GTAC'C
Cuts at: 2302 2864 2302
Size: 562 4977
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4653 4837 1305
Size: 3348 184 2007
Fragments arranged by size:
3348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 3497 3856 4015 518
Size: 648 1021 1310 359 159 2042
Fragments arranged by size:
2042 1310 1021 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2955 3632 4780 5025 1606
Size: 1349 677 1148 245 2120
Fragments arranged by size:
2120 1349 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4048
Fragments arranged by size:
4048 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5539
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5539
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5539
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2891 830
Size: 2061 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2887 4022 4703 4894 826
Size: 219 1842 1135 681 191 1471
Fragments arranged by size:
1842 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2866 1872
Size: 994 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5
Size: 47 3411 2081
Fragments arranged by size:
3411 2081 47
StuI AGG'CCT
Cuts at: 1517 2829 1517
Size: 1312 4227
StyI C'CwwG_G
Cuts at: 513 1065 2004 2438 2978 513
Size: 552 939 434 540 3074
Fragments arranged by size:
3074 939 552 540 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3566 3583 3736 3921 4366 325
Size: 1255 1986 17 153 185 445 1498
Fragments arranged by size:
1986 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2875 2875
Size: 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2864 1870
Size: 994 4545
XmnI GAAnn'nnTTC
Cuts at: 2023 2178 3668 2023
Size: 155 1490 3894
Fragments arranged by size:
3894 1490 155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI