Restriction map for
pFN21AE2420
(Circular) (Six-base) MAPSORT of: pFN21AE2420.seq Check: 6012 from: 1 to: 10720
pFN21AE2420 10720 bp
With 182 enzymes: SgfI *
November 2, 2012 18:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 7309 7309
Size: 10720
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8530 278
Size: 53 83 186 936 6994 2468
Fragments arranged by size:
6994 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4789 6257 8063 1937
Size: 2852 1468 1806 4594
Fragments arranged by size:
4594 2852 1806 1468
Acc65I G'GTAC_C
Cuts at: 3160 8041 3160
Size: 4881 5839
AclI AA'CG_TT
Cuts at: 1563 8847 9220 1563
Size: 7284 373 3063
Fragments arranged by size:
7284 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 3713 4119 4736 6011 6213 7130 7616
Size: 597 1484 406 617 1275 202 917 486
Cuts at: 7616 7715 8783 10167 10435 1632
Size: 99 1068 1384 268 1917
Fragments arranged by size:
1917 1484 1384 1275 1068 917 617 597
486 406 268 202 99
AfeI AGC'GCT
Cuts at: 2153 2153
Size: 10720
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10523
AflIII A'CryG_T
Cuts at: 1203 3755 5893 9619 10596 1203
Size: 2552 2138 3726 977 1327
Fragments arranged by size:
3726 2552 2138 1327 977
AgeI A'CCGG_T
Cuts at: 5884 9551 5884
Size: 3667 7053
AhdI GACnn_n'nnGTC
Cuts at: 3845 7947 9449 3845
Size: 4102 1502 5116
Fragments arranged by size:
5116 4102 1502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10720
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10720
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3930 3962 6810 6842 7671 7703 3930
Size: 32 2848 32 829 32 6947
Fragments arranged by size:
6947 2848 829 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3227 4025 4991 5326 7702 8463 10035 3227
Size: 798 966 335 2376 761 1572 3912
Fragments arranged by size:
3912 2376 1572 966 798 761 335
ApaI G_GGCC'C
Cuts at: 4682 4682
Size: 10720
ApaLI G'TGCA_C
Cuts at: 8777 9933 8777
Size: 1156 9564
ApoI r'AATT_y
Cuts at: 1493 2216 2615 3135 3239 3557 3677 3728 4478
Size: 723 399 520 104 318 120 51 750
Cuts at: 4478 4553 4815 5617 5672 6689 7602 8029 8255
Size: 75 262 802 55 1017 913 427 226
Cuts at: 8255 8407 10516 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1017 913 802 750 723 520
427 399 318 262 226 152 120 104
75 55 51
AseI AT'TA_AT
Cuts at: 160 1032 2447 3443 5820 6219 9274 160
Size: 872 1415 996 2377 399 3055 1606
Fragments arranged by size:
3055 2377 1606 1415 996 872 399
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10720
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 7434 8045 1870
Size: 73 180 5311 611 4545
Fragments arranged by size:
5311 4545 611 180 73
AvrII C'CTAG_G
Cuts at: 6008 6008
Size: 10720
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6037 6070 6037
Size: 33 10687
BamHI G'GATC_C
Cuts at: 8050 8417 8050
Size: 367 10353
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 5068 7057 8041 9496
Size: 325 217 627 1373 1908 1989 984 1455
Cuts at: 9496 618
Size: 1842
Fragments arranged by size:
1989 1908 1842 1455 1373 984 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2124 4248 4682 6789 7684 8039 1391
Size: 733 2124 434 2107 895 355 4072
Fragments arranged by size:
4072 2124 2107 895 733 434 355
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2725 3471 4429 4484 4749 5087 7355
Size: 971 826 746 958 55 265 338 2268
Cuts at: 7355 928
Size: 4293
Fragments arranged by size:
4293 2268 971 958 826 746 338 265
55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8911 8945 1903
Size: 34 6974 34 3678
Fragments arranged by size:
6974 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6398 8612 9822 6398
Size: 2214 1210 7296
Fragments arranged by size:
7296 2214 1210
BclI T'GATC_A
Cuts at: 1548 2644 5395 5863 1548
Size: 1096 2751 468 6405
Fragments arranged by size:
6405 2751 1096 468
BfrBI ATG'CAT
Cuts at: 5203 6140 5203
Size: 937 9783
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9331 136
Size: 107 122 71 631 605 7659 1525
Fragments arranged by size:
7659 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6550 7370 7723 10715 6550
Size: 820 353 2992 6555
Fragments arranged by size:
6555 2992 820 353
BlpI GC'TnA_GC
Cuts at: 8137 8137
Size: 10720
Bme1580I G_kGCm'C
Cuts at: 1790 4682 8781 9937 1790
Size: 2892 4099 1156 2573
Fragments arranged by size:
4099 2892 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3758 7286 1318
Size: 2440 3528 4752
Fragments arranged by size:
4752 3528 2440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2602 5094 8451
Size: 416 53 83 399 24 1185 2492 3357
Cuts at: 8451 9409 442
Size: 958 1753
Fragments arranged by size:
3357 2492 1753 1185 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 5922 1056
Size: 4866 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4559 7668 9380 1239
Size: 3320 3109 1712 2579
Fragments arranged by size:
3320 3109 2579 1712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3407 3617 8845 9710 10008 3407
Size: 210 5228 865 298 4119
Fragments arranged by size:
5228 4119 865 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9383 882
Size: 617 7884 2219
Fragments arranged by size:
7884 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6230 7898 8511 493
Size: 711 5026 1668 613 2702
Fragments arranged by size:
5026 2702 1668 711 613
BsaBI GATnn'nnATC
Cuts at: 6555 7596 10714 6555
Size: 1041 3118 6561
Fragments arranged by size:
6561 3118 1041
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 8527 8909 9544
Size: 53 83 186 936 537 6457 382 635
Cuts at: 9544 275
Size: 1451
Fragments arranged by size:
6457 1451 936 635 537 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5884 7810 9153 9551 9825 9972 10383 5884
Size: 1926 1343 398 274 147 411 6221
Fragments arranged by size:
6221 1926 1343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3252 3282 3930 3960 5502
Size: 30 165 30 1849 30 648 30 1542
Cuts at: 5502 5532 7410 7440 7671 7701 1178
Size: 30 1878 30 231 30 4197
Fragments arranged by size:
4197 1878 1849 1542 648 231 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2954 3474 3954 7635 1186
Size: 883 885 520 480 3681 4271
Fragments arranged by size:
4271 3681 885 883 520 480
BseYI C'CCAG_C
Cuts at: 5594 6277 9923 10419 5594
Size: 683 3646 496 5895
Fragments arranged by size:
5895 3646 683 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 7266 1163
Size: 573 5530 4617
Fragments arranged by size:
5530 4617 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8170 8931 9959 1540
Size: 275 186 6169 761 1028 2301
Fragments arranged by size:
6169 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 4248 4930 7684 8781 8866 9937 2124
Size: 2124 682 2754 1097 85 1071 2907
Fragments arranged by size:
2907 2754 2124 1097 1071 682 85
BsmI GAATG_Cn'
Cuts at: 3521 8231 8324 3521
Size: 4710 93 5917
Fragments arranged by size:
5917 4710 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6119 7931 7966 6119
Size: 1812 35 8873
Fragments arranged by size:
8873 1812 35
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 4248 4682 4930 6789 7684 8039
Size: 399 334 2124 434 248 1859 895 355
Cuts at: 8039 8781 8866 9937 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 2124 1859 1071 895 742 434 399
355 334 248 85
BspEI T'CCGG_A
Cuts at: 7810 10383 7810
Size: 2573 8147
BspHI T'CATG_A
Cuts at: 3703 3913 6720 8609 10339 3703
Size: 210 2807 1889 1730 4084
Fragments arranged by size:
4084 2807 1889 1730 210
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 2307 2545 2779 7309 8075 844
Size: 1287 176 238 234 4530 766 3489
Fragments arranged by size:
4530 3489 1287 766 238 234 176
BsrBI CCG'CTC
Cuts at: 1394 1823 8607 1394
Size: 429 6784 3507
Fragments arranged by size:
6784 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3963 4197 7913 9215 9389 65
Size: 1183 2715 234 3716 1302 174 1396
Fragments arranged by size:
3716 2715 1396 1302 1183 234 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 5884 9364 9551 1700
Size: 110 320 3754 3480 187 2869
Fragments arranged by size:
3754 3480 2869 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10720
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10720
BssSI C'ACGA_G
Cuts at: 2792 8780 9792 2792
Size: 5988 1012 3720
Fragments arranged by size:
5988 3720 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3800 7324 794
Size: 3006 3524 4190
Fragments arranged by size:
4190 3524 3006
BstBI TT'CG_AA
Cuts at: 7606 9524 7606
Size: 1918 8802
BstEII G'GTnAC_C
Cuts at: 1184 4778 1184
Size: 3594 7126
BstXI CCAn_nnnn'nTGG
Cuts at: 7513 7513
Size: 10720
BstYI r'GATC_y
Cuts at: 1972 3592 4498 4988 5701 6550 7370 7420 7723
Size: 1620 906 490 713 849 820 50 303
Cuts at: 7723 7777 8050 8417 8802 8819 10271 10357 10369
Size: 54 273 367 385 17 1452 86 12
Cuts at: 10369 10715 1972
Size: 346 1977
Fragments arranged by size:
1977 1620 1452 906 849 820 713 490
385 367 346 303 273 86 54 50
17 12
Bsu36I CC'TnA_GG
Cuts at: 3179 3966 3179
Size: 787 9933
BtgI C'CryG_G
Cuts at: 513 1065 2004 4216 513
Size: 552 939 2212 7017
Fragments arranged by size:
7017 2212 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7232 487
Size: 683 56 6006 3975
Fragments arranged by size:
6006 3975 683 56
BtsI GCAGTG_nn'
Cuts at: 2553 7165 7577 8236 9030 9050 2553
Size: 4612 412 659 794 20 4223
Fragments arranged by size:
4612 4223 794 659 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10720
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10685
DraI TTT'AAA
Cuts at: 2688 3642 4333 5457 5928 7408 8024 2688
Size: 954 691 1124 471 1480 616 5384
Fragments arranged by size:
5384 1480 1124 954 691 616 471
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7259 9727 809
Size: 507 5943 2468 1802
Fragments arranged by size:
5943 2468 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 5789 7530
Size: 54 1425 50 275 18 244 3715 1741
Cuts at: 7530 8167 9056 10685 8
Size: 637 889 1629 43
Fragments arranged by size:
3715 1741 1629 1425 889 637 275 244
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8167 1537
Size: 275 6355 4090
Fragments arranged by size:
6355 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2557 3488 5821 6030 7143 7359 8650 1353
Size: 1204 931 2333 209 1113 216 1291 3423
Fragments arranged by size:
3423 2333 1291 1204 1113 931 216 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2079 9307 9681 9827 387
Size: 1064 138 441 49 7228 374 146 1280
Fragments arranged by size:
7228 1280 1064 441 374 146 138 49
EcoICRI GAG'CTC
Cuts at: 2122 4246 7682 2122
Size: 2124 3436 5160
Fragments arranged by size:
5160 3436 2124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 3713 4119 4559 4736 6011 6213
Size: 393 597 1484 406 440 177 1275 202
Cuts at: 6213 7130 7616 7668 7715 8783 9380 10167 10435
Size: 917 486 52 47 1068 597 787 268
Cuts at: 10435 1239
Size: 1524
Fragments arranged by size:
1524 1484 1275 1068 917 787 597 597
486 440 406 393 268 202 177 52
47
EcoO109I rG'GnC_Cy
Cuts at: 2037 3785 4678 4679 5684 6065 6537 7627 2037
Size: 1748 893 1 1005 381 472 1090 5130
Fragments arranged by size:
5130 1748 1090 1005 893 472 381 1
EcoRI G'AATT_C
Cuts at: 3239 6689 7602 8029 8407 3239
Size: 3450 913 427 378 5552
Fragments arranged by size:
5552 3450 913 427 378
EcoRV GAT'ATC
Cuts at: 3531 3531
Size: 10720
FspI TGC'GCA
Cuts at: 9226 9226
Size: 10720
HaeII r_GCGC'y
Cuts at: 1678 2155 9867 1678
Size: 477 7712 2531
Fragments arranged by size:
7712 2531 477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3831 3863 4067 4099 5149
Size: 32 135 32 2259 32 204 32 1050
Cuts at: 5149 5181 5387 5419 6887 6919 7171 7203 7410
Size: 32 206 32 1468 32 252 32 207
Cuts at: 7410 7442 7933 7965 9361 9393 9435 9467 1373
Size: 32 491 32 1396 32 42 32 2626
Fragments arranged by size:
2626 2259 1468 1396 1050 491 252 207
206 204 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3175 4304 4571 4790 7446 8064
Size: 988 281 1237 1129 267 219 2656 618
Cuts at: 8064 8310 669
Size: 246 3079
Fragments arranged by size:
3079 2656 1237 1129 988 618 281 267
246 219
HindIII A'AGCT_T
Cuts at: 748 4199 748
Size: 3451 7269
HpaI GTT'AAC
Cuts at: 7446 8310 7446
Size: 864 9856
KpnI G_GTAC'C
Cuts at: 3164 8045 3164
Size: 4881 5839
MfeI C'AATT_G
Cuts at: 3510 3999 5589 7004 8319 3510
Size: 489 1590 1415 1315 5911
Fragments arranged by size:
5911 1590 1415 1315 489
MluI A'CGCG_T
Cuts at: 10596 10596
Size: 10720
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2722 4495 5581 9834 10018 1305
Size: 1417 1773 1086 4253 184 2007
Fragments arranged by size:
4253 2007 1773 1417 1086 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5791 10
Size: 54 1425 343 3959 4939
Fragments arranged by size:
4939 3959 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5169 5813 6242 8678 9037 9196 518
Size: 648 4003 644 429 2436 359 159 2042
Fragments arranged by size:
4003 2436 2042 648 644 429 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 3227 3844 6506 8136 8813 9961 10206
Size: 406 1215 617 2662 1630 677 1148 245
Cuts at: 10206 1606
Size: 2120
Fragments arranged by size:
2662 2120 1630 1215 1148 677 617 406
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4216 513
Size: 552 939 2212 7017
Fragments arranged by size:
7017 2212 939 552
NdeI CA'TA_TG
Cuts at: 387 3799 387
Size: 3412 7308
NheI G'CTAG_C
Cuts at: 1052 5918 1052
Size: 4866 5854
NotI GC'GGCC_GC
Cuts at: 8167 8167
Size: 10720
NsiI A_TGCA'T
Cuts at: 5205 6142 5205
Size: 937 9783
NspI r_CATG'y
Cuts at: 1132 1229 2392 3202 5897 8078 9623 1132
Size: 97 1163 810 2695 2181 1545 2229
Fragments arranged by size:
2695 2229 2181 1545 1163 810 97
PasI CC'CwG_GG
Cuts at: 1348 3789 1348
Size: 2441 8279
PciI A'CATG_T
Cuts at: 5893 9619 5893
Size: 3726 6994
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10720
PfoI T'CCnGG_A
Cuts at: 4204 5689 6748 4204
Size: 1485 1059 8176
Fragments arranged by size:
8176 1485 1059
PmeI GTTT'AAAC
Cuts at: 8024 8024
Size: 10720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3930 3962 7671 7703 8789 8821 10302 10334 3930
Size: 32 3709 32 1086 32 1481 32 4316
Fragments arranged by size:
4316 3709 1481 1086 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3785 5684 6065 6537 3785
Size: 1899 381 472 7968
Fragments arranged by size:
7968 1899 472 381
PshAI GACnn'nnGTC
Cuts at: 1613 6542 1613
Size: 4929 5791
PsiI TTA'TAA
Cuts at: 4710 5522 8003 8290 4710
Size: 812 2481 287 7140
Fragments arranged by size:
7140 2481 812 287
PspOMI G'GGCC_C
Cuts at: 4678 4678
Size: 10720
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5301 5333 7008 7040 5301
Size: 32 1675 32 8981
Fragments arranged by size:
8981 1675 32 32
PstI C_TGCA'G
Cuts at: 830 2142 2776 8072 830
Size: 1312 634 5296 3478
Fragments arranged by size:
5296 3478 1312 634
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10720
PvuII CAG'CTG
Cuts at: 3227 3844 6506 3227
Size: 617 2662 7441
Fragments arranged by size:
7441 2662 617
SacI G_AGCT'C
Cuts at: 2124 4248 7684 2124
Size: 2124 3436 5160
Fragments arranged by size:
5160 3436 2124
SalI G'TCGA_C
Cuts at: 1936 4788 8062 1936
Size: 2852 3274 4594
Fragments arranged by size:
4594 3274 2852
SbfI CC_TGCA'GG
Cuts at: 8072 8072
Size: 10720
ScaI AGT'ACT
Cuts at: 3005 6763 8968 10680 3005
Size: 3758 2205 1712 3045
Fragments arranged by size:
3758 3045 2205 1712
SexAI A'CCwGG_T
Cuts at: 4774 4774
Size: 10720
SfcI C'TryA_G
Cuts at: 826 1045 2138 2303 2772 3933 5318 8068 9203
Size: 219 1093 165 469 1161 1385 2750 1135
Cuts at: 9203 9884 10075 826
Size: 681 191 1471
Fragments arranged by size:
2750 1471 1385 1161 1135 1093 681 469
219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10720
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10720
SmaI CCC'GGG
Cuts at: 1872 8047 1872
Size: 6175 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3386 3596 7434 8824 9725 9987
Size: 197 926 1443 210 3838 1390 901 262
Cuts at: 9987 820
Size: 1553
Fragments arranged by size:
3838 1553 1443 1390 926 901 262 210
197
SnaBI TAC'GTA
Cuts at: 493 7898 8511 493
Size: 7405 613 2702
Fragments arranged by size:
7405 2702 613
SpeI A'CTAG_T
Cuts at: 152 4573 152
Size: 4421 6299
SphI G_CATG'C
Cuts at: 1132 8078 1132
Size: 6946 3774
SspI AAT'ATT
Cuts at: 5 52 2399 3124 4348 5011 5127 6421 6770
Size: 47 2347 725 1224 663 116 1294 349
Cuts at: 6770 7033 8644 5
Size: 263 1611 2081
Fragments arranged by size:
2347 2081 1611 1294 1224 725 663 349
263 116 47
StuI AGG'CCT
Cuts at: 1517 3412 4132 1517
Size: 1895 720 8105
Fragments arranged by size:
8105 1895 720
StyI C'CwwG_G
Cuts at: 513 1065 2004 4216 4674 6008 6540 7306 8159
Size: 552 939 2212 458 1334 532 766 853
Cuts at: 8159 513
Size: 3074
Fragments arranged by size:
3074 2212 1334 939 853 766 552 532
458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 8747 8764 8917 9102 9547 325
Size: 1255 719 6448 17 153 185 445 1498
Fragments arranged by size:
6448 1498 1255 719 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2088 3003 3479
Size: 274 80 33 51 1443 111 915 476
Cuts at: 3479 5485 6761 7133 8966 10678 96
Size: 2006 1276 372 1833 1712 138
Fragments arranged by size:
2006 1833 1712 1443 1276 915 476 372
274 138 111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3520 3552 5176 5208 6675 6707 10680 10712 3520
Size: 32 1624 32 1467 32 3973 32 3528
Fragments arranged by size:
3973 3528 1624 1467 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10720
XbaI T'CTAG_A
Cuts at: 8056 8056
Size: 10720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6538 6538
Size: 10720
XhoI C'TCGA_G
Cuts at: 1943 7434 1943
Size: 5491 5229
XmaI C'CCGG_G
Cuts at: 1870 8045 1870
Size: 6175 4545
XmnI GAAnn'nnTTC
Cuts at: 2916 4347 7074 8849 2916
Size: 1431 2727 1775 4787
Fragments arranged by size:
4787 2727 1775 1431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8528 276
Size: 53 83 186 936 6994 2468
Fragments arranged by size:
6994 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI