Restriction map for
pFN21AE2415
(Circular) (Six-base) MAPSORT of: pFN21AE2415.seq Check: 5406 from: 1 to: 6082
pFN21AE2415 6082 bp
With 182 enzymes: SgfI *
July 10, 2012 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2449 2449
Size: 6082
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2310 3330 3425 1937
Size: 373 1020 95 4594
Fragments arranged by size:
4594 1020 373 95
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 4145 5529 5797 1632
Size: 402 2111 1384 268 1917
Fragments arranged by size:
2111 1917 1384 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 3264 3379 4981 5958 1203
Size: 2061 115 1602 977 1327
Fragments arranged by size:
2061 1602 1327 977 115
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 2930 3200 1166
Size: 1764 270 4048
Fragments arranged by size:
4048 1764 270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 3825 5397 3825
Size: 1572 4510
ApaLI G'TGCA_C
Cuts at: 4139 5295 4139
Size: 1156 4926
ApoI r'AATT_y
Cuts at: 1493 2044 3391 3617 3769 5878 1493
Size: 551 1347 226 152 2109 1697
Fragments arranged by size:
2109 1697 1347 551 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 3407 1870
Size: 73 1464 4545
Fragments arranged by size:
4545 1464 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2798 2831 2798
Size: 33 6049
BamHI G'GATC_C
Cuts at: 3348 3412 3779 3348
Size: 64 367 5651
Fragments arranged by size:
5651 367 64
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 3403 4858 618
Size: 325 217 627 590 1026 1455 1842
Fragments arranged by size:
1842 1455 1026 627 590 325 217
BanII G_rGCy'C
Cuts at: 1391 2389 2428 3401 1391
Size: 998 39 973 4072
Fragments arranged by size:
4072 998 973 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2171 2632 928
Size: 971 272 461 4378
Fragments arranged by size:
4378 971 461 272
BbvCI CC'TCA_GC
Cuts at: 3025 3224 3025
Size: 199 5883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 3300 1548
Size: 1752 4330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4693 136
Size: 107 122 71 631 605 3021 1525
Fragments arranged by size:
3021 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6077 6077
Size: 6082
BlpI GC'TnA_GC
Cuts at: 3499 3499
Size: 6082
Bme1580I G_kGCm'C
Cuts at: 1790 4143 5299 1790
Size: 2353 1156 2573
Fragments arranged by size:
2573 2353 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2114 2175 3170
Size: 416 53 83 399 24 697 61 995
Cuts at: 3170 3813 4771 442
Size: 643 958 1753
Fragments arranged by size:
1753 995 958 697 643 416 399 83
61 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2414 3151 4742 1239
Size: 866 309 737 1591 2579
Fragments arranged by size:
2579 1591 866 737 309
Bpu10I CC'TnA_GC
Cuts at: 3025 3224 3025
Size: 199 5883
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 2981 4207 5072 5370 2218
Size: 763 1226 865 298 2930
Fragments arranged by size:
2930 1226 865 763 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4745 882
Size: 617 3246 2219
Fragments arranged by size:
3246 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 3265 3873 493
Size: 711 1188 873 608 2702
Fragments arranged by size:
2702 1188 873 711 608
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3889 4271 4906 275
Size: 53 83 186 936 2356 382 635 1451
Fragments arranged by size:
2356 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4515 4913 5187 5334 5745 4515
Size: 398 274 147 411 4852
Fragments arranged by size:
4852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2376 2406 1178
Size: 30 165 30 973 30 4854
Fragments arranged by size:
4854 973 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3013 1186
Size: 1827 4255
BseYI C'CCAG_C
Cuts at: 3360 5285 5781 3360
Size: 1925 496 3661
Fragments arranged by size:
3661 1925 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2208 1163
Size: 573 472 5037
Fragments arranged by size:
5037 573 472
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3532 4293 5321 1540
Size: 275 186 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2443 4143 4228 5299 2443
Size: 1700 85 1071 3226
Fragments arranged by size:
3226 1700 1071 85
BsmI GAATG_Cn'
Cuts at: 3593 3686 3593
Size: 93 5989
BsmBI CGTCTCn'nnnn_
Cuts at: 2661 2661
Size: 6082
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2389 2428 2443 3401 4143 4228 5299
Size: 399 599 39 15 958 742 85 1071
Cuts at: 5299 1391
Size: 2174
Fragments arranged by size:
2174 1071 958 742 599 399 85 39
15
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3971 5701 3971
Size: 1730 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2449 3437 844
Size: 1605 988 3489
Fragments arranged by size:
3489 1605 988
BsrBI CCG'CTC
Cuts at: 1394 1823 3969 1394
Size: 429 2146 3507
Fragments arranged by size:
3507 2146 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3124 4577 4751 65
Size: 1183 1876 1453 174 1396
Fragments arranged by size:
1876 1453 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4726 4913 1700
Size: 110 2916 187 2869
Fragments arranged by size:
2916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3185 96
Size: 3089 2993
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 4142 5154 4142
Size: 1012 5070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2447 794
Size: 1653 4429
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 3348 3412 3779 4164 4181 5633 5719 5731
Size: 1376 64 367 385 17 1452 86 12
Cuts at: 5731 6077 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1376 385 367 346 86 64
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4591
Fragments arranged by size:
4591 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2762 3598 4392 4412 2762
Size: 836 794 20 4432
Fragments arranged by size:
4432 836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3529 4418 6047
Size: 54 1425 50 275 18 1699 889 1629
Cuts at: 6047 8
Size: 43
Fragments arranged by size:
1699 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3209 4012 1353
Size: 1856 803 3423
Fragments arranged by size:
3423 1856 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3341 4669 5043 5189 387
Size: 1064 138 1752 1328 374 146 1280
Fragments arranged by size:
1752 1328 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2105 2414 3151 4145 4742 5529
Size: 393 402 71 309 737 994 597 787
Cuts at: 5529 5797 1239
Size: 268 1524
Fragments arranged by size:
1524 994 787 737 597 402 393 309
268 71
EcoRI G'AATT_C
Cuts at: 2044 3391 3769 2044
Size: 1347 378 4357
Fragments arranged by size:
4357 1347 378
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 5229 1678
Size: 3551 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2531 2563 2658 2690 4723
Size: 32 135 32 959 32 95 32 2033
Cuts at: 4723 4755 4797 4829 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2033 959 135 95 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3127 3426 3672 669
Size: 988 281 1189 299 246 3079
Fragments arranged by size:
3079 1189 988 299 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6082
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4260
Fragments arranged by size:
4260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2930 3200 4040 4399 4558 518
Size: 648 1764 270 840 359 159 2042
Fragments arranged by size:
2042 1764 840 648 359 270 159
MspA1I CmG'CkG
Cuts at: 1606 3498 4175 5323 5568 1606
Size: 1892 677 1148 245 2120
Fragments arranged by size:
2120 1892 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4591
Fragments arranged by size:
4591 939 552
NdeI CA'TA_TG
Cuts at: 387 3046 387
Size: 2659 3423
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 3440 4985 1132
Size: 97 2211 1545 2229
Fragments arranged by size:
2229 2211 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6082
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3199 1108
Size: 2091 3991
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PmlI CAC'GTG
Cuts at: 2392 2392
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 2663 3652 2663
Size: 989 5093
PstI C_TGCA'G
Cuts at: 830 2589 3259 3434 830
Size: 1759 670 175 3478
Fragments arranged by size:
3478 1759 670 175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SfcI C'TryA_G
Cuts at: 826 1045 2585 3255 3430 4565 5246 5437 826
Size: 219 1540 670 175 1135 681 191 1471
Fragments arranged by size:
1540 1471 1135 681 670 219 191 175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 3409 1872
Size: 1537 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2197 2996 4186 5087 5349 820
Size: 197 926 254 799 1190 901 262 1553
Fragments arranged by size:
1553 1190 926 901 799 262 254 197
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 2304 4006 5
Size: 47 2252 1702 2081
Fragments arranged by size:
2252 2081 1702 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6082
StyI C'CwwG_G
Cuts at: 513 1065 2004 3521 513
Size: 552 939 1517 3074
Fragments arranged by size:
3074 1517 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4109 4126 4279 4464 4909 325
Size: 1255 2529 17 153 185 445 1498
Fragments arranged by size:
2529 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3082 3185 4328
Size: 274 80 33 51 1443 1105 103 1143
Cuts at: 4328 6040 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1143 1105 274 138 103 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2376 2408 6042 6074 2376
Size: 32 3634 32 2384
Fragments arranged by size:
3634 2384 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6082
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 3407 1870
Size: 1537 4545
XmnI GAAnn'nnTTC
Cuts at: 3097 3218 4211 3097
Size: 121 993 4968
Fragments arranged by size:
4968 993 121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI