Restriction map for
pFN21AE2412
(Circular) (Six-base) MAPSORT of: pFN21AE2412.seq Check: 8903 from: 1 to: 5233
pFN21AE2412 5233 bp
With 182 enzymes: SgfI *
May 24, 2012 15:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3043 278
Size: 53 83 186 936 1507 2468
Fragments arranged by size:
2468 1507 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2576 1937
Size: 639 4594
Acc65I G'GTAC_C
Cuts at: 2554 2554
Size: 5233
AclI AA'CG_TT
Cuts at: 1563 3360 3733 1563
Size: 1797 373 3063
Fragments arranged by size:
3063 1797 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3296 4680 4948 1632
Size: 1664 1384 268 1917
Fragments arranged by size:
1917 1664 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5036
AflIII A'CryG_T
Cuts at: 1203 4132 5109 1203
Size: 2929 977 1327
Fragments arranged by size:
2929 1327 977
AgeI A'CCGG_T
Cuts at: 2530 4064 2530
Size: 1534 3699
AhdI GACnn_n'nnGTC
Cuts at: 3962 3962
Size: 5233
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 2123
Size: 32 5201
AlwNI CAG_nnn'CTG
Cuts at: 2976 4548 2976
Size: 1572 3661
ApaLI G'TGCA_C
Cuts at: 3290 4446 3290
Size: 1156 4077
ApoI r'AATT_y
Cuts at: 1493 2542 2768 2920 5029 1493
Size: 1049 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3787 160
Size: 872 2755 1606
Fragments arranged by size:
2755 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5233
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2110 2558 1870
Size: 73 124 43 448 4545
Fragments arranged by size:
4545 448 124 73 43
BamHI G'GATC_C
Cuts at: 2563 2930 2563
Size: 367 4866
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2554 4009 618
Size: 325 217 627 327 440 1455 1842
Fragments arranged by size:
1842 1455 627 440 327 325 217
BanII G_rGCy'C
Cuts at: 1391 2552 1391
Size: 1161 4072
BbeI G_GCGC'C
Cuts at: 2118 2118
Size: 5233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2329 928
Size: 971 430 3832
Fragments arranged by size:
3832 971 430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3424 3458 1903
Size: 34 1487 34 3678
Fragments arranged by size:
3678 1487 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3125 4335 3125
Size: 1210 4023
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5233
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3844 136
Size: 107 122 71 631 605 337 1835 1525
Fragments arranged by size:
1835 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5228 5228
Size: 5233
BlpI GC'TnA_GC
Cuts at: 2650 2650
Size: 5233
Bme1580I G_kGCm'C
Cuts at: 1790 3294 4450 1790
Size: 1504 1156 2573
Fragments arranged by size:
2573 1504 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5233
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2964 3922 442
Size: 416 53 83 399 24 1547 958 1753
Fragments arranged by size:
1753 1547 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 3893 1239
Size: 911 1743 2579
Fragments arranged by size:
2579 1743 911
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3358 4223 4521 3358
Size: 865 298 4070
Fragments arranged by size:
4070 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3896 882
Size: 617 2397 2219
Fragments arranged by size:
2397 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3024 493
Size: 711 1820 2702
Fragments arranged by size:
2702 1820 711
BsaBI GATnn'nnATC
Cuts at: 5227 5227
Size: 5233
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3040 3422 4057
Size: 53 83 186 936 582 925 382 635
Cuts at: 4057 275
Size: 1451
Fragments arranged by size:
1451 936 925 635 582 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2530 3666 4064 4338 4485 4896 2530
Size: 1136 398 274 147 411 2867
Fragments arranged by size:
2867 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2010 2040 2123 2153 1178
Size: 30 165 30 607 30 83 30 4258
Fragments arranged by size:
4258 607 165 83 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2234 1186
Size: 975 73 4185
Fragments arranged by size:
4185 975 73
BseYI C'CCAG_C
Cuts at: 4436 4932 4436
Size: 496 4737
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4660
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2531 2683 3444 4472 1540
Size: 275 186 12 518 152 761 1028 2301
Fragments arranged by size:
2301 1028 761 518 275 186 152 12
BsiHKAI G_wGCw'C
Cuts at: 3294 3379 4450 3294
Size: 85 1071 4077
Fragments arranged by size:
4077 1071 85
BsmI GAATG_Cn'
Cuts at: 2744 2837 2744
Size: 93 5140
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2552 3294 3379 4450 1391
Size: 399 762 742 85 1071 2174
Fragments arranged by size:
2174 1071 762 742 399 85
BspEI T'CCGG_A
Cuts at: 4896 4896
Size: 5233
BspHI T'CATG_A
Cuts at: 3122 4852 3122
Size: 1730 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2588 844
Size: 1744 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3120 1394
Size: 429 1297 3507
Fragments arranged by size:
3507 1297 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2392 3728 3902 65
Size: 1183 1144 1336 174 1396
Fragments arranged by size:
1396 1336 1183 1144 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 2084 2530 3877 4064 1700
Size: 110 202 72 446 1347 187 2869
Fragments arranged by size:
2869 1347 446 202 187 110 72
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5233
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5233
BssSI C'ACGA_G
Cuts at: 3293 4305 3293
Size: 1012 4221
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 794
Size: 1595 3638
BstBI TT'CG_AA
Cuts at: 4037 4037
Size: 5233
BstEII G'GTnAC_C
Cuts at: 1184 2071 1184
Size: 887 4346
BstYI r'GATC_y
Cuts at: 1972 2563 2930 3315 3332 4784 4870 4882 5228
Size: 591 367 385 17 1452 86 12 346
Cuts at: 5228 1972
Size: 1977
Fragments arranged by size:
1977 1452 591 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4681
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4494
Fragments arranged by size:
4494 683 56
BtsI GCAGTG_nn'
Cuts at: 2749 3543 3563 2749
Size: 794 20 4419
Fragments arranged by size:
4419 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5198
DraI TTT'AAA
Cuts at: 2537 2537
Size: 5233
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4240 809
Size: 507 2924 1802
Fragments arranged by size:
2924 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2680 3569
Size: 54 1425 50 275 18 180 670 889
Cuts at: 3569 5198 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 670 275 180 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2680 1537
Size: 275 198 670 4090
Fragments arranged by size:
4090 670 275 198
EarI CTCTTCn'nnn_
Cuts at: 1353 3163 1353
Size: 1810 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2031 3820 4194 4340 387
Size: 1064 138 442 1789 374 146 1280
Fragments arranged by size:
1789 1280 1064 442 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 3296 3893 4680 4948 1239
Size: 393 518 1146 597 787 268 1524
Fragments arranged by size:
1524 1146 787 597 518 393 268
EcoRI G'AATT_C
Cuts at: 2542 2920 2542
Size: 378 4855
FspI TGC'GCA
Cuts at: 3739 3739
Size: 5233
HaeII r_GCGC'y
Cuts at: 1678 2118 4380 1678
Size: 440 2262 2531
Fragments arranged by size:
2531 2262 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2010 2042 3874 3906 3948
Size: 32 135 32 438 32 1832 32 42
Cuts at: 3948 3980 1373
Size: 32 2626
Fragments arranged by size:
2626 1832 438 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2577 2823 669
Size: 988 281 639 246 3079
Fragments arranged by size:
3079 988 639 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5233
HpaI GTT'AAC
Cuts at: 2823 2823
Size: 5233
KasI G'GCGC_C
Cuts at: 2114 2114
Size: 5233
KpnI G_GTAC'C
Cuts at: 2558 2558
Size: 5233
MfeI C'AATT_G
Cuts at: 2832 2832
Size: 5233
MluI A'CGCG_T
Cuts at: 5109 5109
Size: 5233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4347 4531 1305
Size: 3042 184 2007
Fragments arranged by size:
3042 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3411
Fragments arranged by size:
3411 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 3191 3550 3709 518
Size: 648 1047 978 359 159 2042
Fragments arranged by size:
2042 1047 978 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2649 3326 4474 4719 1606
Size: 1043 677 1148 245 2120
Fragments arranged by size:
2120 1148 1043 677 245
NaeI GCC'GGC
Cuts at: 2014 2086 2014
Size: 72 5161
NarI GG'CG_CC
Cuts at: 2115 2115
Size: 5233
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4681
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5233
NgoMIV G'CCGG_C
Cuts at: 2012 2084 2012
Size: 72 5161
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5233
NotI GC'GGCC_GC
Cuts at: 2680 2680
Size: 5233
NspI r_CATG'y
Cuts at: 1132 1229 2260 2591 4136 1132
Size: 97 1031 331 1545 2229
Fragments arranged by size:
2229 1545 1031 331 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5233
PciI A'CATG_T
Cuts at: 4132 4132
Size: 5233
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5233
PfoI T'CCnGG_A
Cuts at: 2304 2304
Size: 5233
PmeI GTTT'AAAC
Cuts at: 2537 2537
Size: 5233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 3302 3334 4815 4847 2123
Size: 32 1147 32 1481 32 2509
Fragments arranged by size:
2509 1481 1147 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5233
PsiI TTA'TAA
Cuts at: 2803 2803
Size: 5233
PstI C_TGCA'G
Cuts at: 830 2585 830
Size: 1755 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5233
SalI G'TCGA_C
Cuts at: 1936 2575 1936
Size: 639 4594
SbfI CC_TGCA'GG
Cuts at: 2585 2585
Size: 5233
ScaI AGT'ACT
Cuts at: 3481 5193 3481
Size: 1712 3521
SfcI C'TryA_G
Cuts at: 826 1045 2581 3716 4397 4588 826
Size: 219 1536 1135 681 191 1471
Fragments arranged by size:
1536 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2116 2116
Size: 5233
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5233
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5233
SmaI CCC'GGG
Cuts at: 1872 2069 2560 1872
Size: 197 491 4545
Fragments arranged by size:
4545 491 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3337 4238 4500 820
Size: 197 926 1394 901 262 1553
Fragments arranged by size:
1553 1394 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3024 493
Size: 2531 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5233
SphI G_CATG'C
Cuts at: 1132 2591 1132
Size: 1459 3774
SspI AAT'ATT
Cuts at: 5 52 3157 5
Size: 47 3105 2081
Fragments arranged by size:
3105 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5233
StyI C'CwwG_G
Cuts at: 513 1065 2672 513
Size: 552 1607 3074
Fragments arranged by size:
3074 1607 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3260 3277 3430 3615 4060 325
Size: 1255 1680 17 153 185 445 1498
Fragments arranged by size:
1680 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3479 5191 96
Size: 274 80 33 51 1443 1502 1712 138
Fragments arranged by size:
1712 1502 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5193 5225 5193
Size: 32 5201
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5233
XbaI T'CTAG_A
Cuts at: 2569 2569
Size: 5233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5233
XmaI C'CCGG_G
Cuts at: 1870 2067 2558 1870
Size: 197 491 4545
Fragments arranged by size:
4545 491 197
XmnI GAAnn'nnTTC
Cuts at: 3362 3362
Size: 5233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3041 276
Size: 53 83 186 936 1507 2468
Fragments arranged by size:
2468 1507 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI