Restriction map for
pFN21AE2408
(Circular) (Six-base) MAPSORT of: pFN21AE2408.seq Check: 4092 from: 1 to: 11209
pFN21AE2408 11209 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4156 4156
Size: 11209
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 9019 278
Size: 53 83 186 936 3061 4422 2468
Fragments arranged by size:
4422 3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2239 3134 4606 8552 1937
Size: 302 895 1472 3946 4594
Fragments arranged by size:
4594 3946 1472 895 302
Acc65I G'GTAC_C
Cuts at: 8530 8530
Size: 11209
AclI AA'CG_TT
Cuts at: 1563 9336 9709 1563
Size: 7773 373 3063
Fragments arranged by size:
7773 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2067 3276 3377 3837 4014 4377 4402 5615
Size: 435 1209 101 460 177 363 25 1213
Cuts at: 5615 5622 5793 6368 6848 6881 7056 8067 8148
Size: 7 171 575 480 33 175 1011 81
Cuts at: 8148 9272 10656 10924 1632
Size: 1124 1384 268 1917
Fragments arranged by size:
1917 1384 1213 1209 1124 1011 575 480
460 435 363 268 177 175 171 101
81 33 25 7
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11012
AflIII A'CryG_T
Cuts at: 1203 5199 10108 11085 1203
Size: 3996 4909 977 1327
Fragments arranged by size:
4909 3996 1327 977
AgeI A'CCGG_T
Cuts at: 10040 10040
Size: 11209
AhdI GACnn_n'nnGTC
Cuts at: 9938 9938
Size: 11209
AleI CACnn'nnGTG
Cuts at: 1166 3120 6920 1166
Size: 1954 3800 5455
Fragments arranged by size:
5455 3800 1954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6505 1184
Size: 5321 5888
AlwNI CAG_nnn'CTG
Cuts at: 4112 5441 7478 8952 10524 4112
Size: 1329 2037 1474 1572 4797
Fragments arranged by size:
4797 2037 1572 1474 1329
ApaLI G'TGCA_C
Cuts at: 6490 7578 9266 10422 6490
Size: 1088 1688 1156 7277
Fragments arranged by size:
7277 1688 1156 1088
ApoI r'AATT_y
Cuts at: 1493 2267 2409 4631 4893 7245 7553 8518 8744
Size: 774 142 2222 262 2352 308 965 226
Cuts at: 8744 8896 11005 1493
Size: 152 2109 1697
Fragments arranged by size:
2352 2222 2109 1697 965 774 308 262
226 152 142
AseI AT'TA_AT
Cuts at: 160 1032 3561 9763 160
Size: 872 2529 6202 1606
Fragments arranged by size:
6202 2529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11209
AvaI C'yCGr_G
Cuts at: 1870 1943 4743 7341 8534 1870
Size: 73 2800 2598 1193 4545
Fragments arranged by size:
4545 2800 2598 1193 73
BamHI G'GATC_C
Cuts at: 8539 8906 8539
Size: 367 10842
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4296 8530 9985 618
Size: 325 217 627 2509 4234 1455 1842
Fragments arranged by size:
4234 2509 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4888 7692 8528 1391
Size: 3497 2804 836 4072
Fragments arranged by size:
4072 3497 2804 836
BbeI G_GCGC'C
Cuts at: 4300 4300
Size: 11209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4549 4990 5202 5603 5976 7572 8061
Size: 971 2650 441 212 401 373 1596 489
Cuts at: 8061 928
Size: 4076
Fragments arranged by size:
4076 2650 1596 971 489 441 401 373
212
BbvCI CC'TCA_GC
Cuts at: 2032 4788 2032
Size: 2756 8453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7559 7593 9400 9434 1903
Size: 34 5622 34 1807 34 3678
Fragments arranged by size:
5622 3678 1807 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5462 8370 9101 10311 5462
Size: 2908 731 1210 6360
Fragments arranged by size:
6360 2908 1210 731
BclI T'GATC_A
Cuts at: 1548 5655 1548
Size: 4107 7102
BfrBI ATG'CAT
Cuts at: 8249 8249
Size: 11209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9820 136
Size: 107 122 71 631 605 8148 1525
Fragments arranged by size:
8148 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6349 7417 11204 6349
Size: 1068 3787 6354
Fragments arranged by size:
6354 3787 1068
BlpI GC'TnA_GC
Cuts at: 4887 5206 8626 4887
Size: 319 3420 7470
Fragments arranged by size:
7470 3420 319
Bme1580I G_kGCm'C
Cuts at: 1790 4071 5852 6494 7582 9270 10426 1790
Size: 2281 1781 642 1088 1688 1156 2573
Fragments arranged by size:
2573 2281 1781 1688 1156 1088 642
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11209
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 2414 3719
Size: 416 53 83 399 24 805 192 1305
Cuts at: 3719 3795 4135 6536 8940 9898 442
Size: 76 340 2401 2404 958 1753
Fragments arranged by size:
2404 2401 1753 1305 958 805 416 399
340 192 83 76 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2133 5607 5844 7029 7668 9869 1239
Size: 795 99 3474 237 1185 639 2201 2579
Fragments arranged by size:
3474 2579 2201 1185 795 639 237 99
Bpu10I CC'TnA_GC
Cuts at: 2032 2254 2305 2617 4788 7691 7944 2032
Size: 222 51 312 2171 2903 253 5297
Fragments arranged by size:
5297 2903 2171 312 253 222 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2755 4384 4662 5746 7257 7268 8087 9334 10199
Size: 1629 278 1084 1511 11 819 1247 865
Cuts at: 10199 10497 2755
Size: 298 3467
Fragments arranged by size:
3467 1629 1511 1247 1084 865 819 298
278 11
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9872 882
Size: 617 8373 2219
Fragments arranged by size:
8373 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 9000 493
Size: 711 7796 2702
Fragments arranged by size:
7796 2702 711
BsaBI GATnn'nnATC
Cuts at: 11203 11203
Size: 11209
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4297 4594 6154 9016
Size: 53 83 186 936 2764 297 1560 2862
Cuts at: 9016 9398 10033 275
Size: 382 635 1451
Fragments arranged by size:
2862 2764 1560 1451 936 635 382 297
186 83 53
BsaWI w'CCGG_w
Cuts at: 9642 10040 10314 10461 10872 9642
Size: 398 274 147 411 9979
Fragments arranged by size:
9979 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5842 5872 6787 6817 7346
Size: 30 165 30 4439 30 915 30 529
Cuts at: 7346 7376 1178
Size: 30 5011
Fragments arranged by size:
5011 4439 915 529 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 3510 4805 5420 5743 5901 6750 6795
Size: 846 1478 1295 615 323 158 849 45
Cuts at: 6795 1186
Size: 5600
Fragments arranged by size:
5600 1478 1295 849 846 615 323 158
45
BseYI C'CCAG_C
Cuts at: 2168 2431 3030 6799 10412 10908 2168
Size: 263 599 3769 3613 496 2469
Fragments arranged by size:
3769 3613 2469 599 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2361 5315 7716 1163
Size: 573 625 2954 2401 4656
Fragments arranged by size:
4656 2954 2401 625 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8659 9420 10448 1540
Size: 275 186 6658 761 1028 2301
Fragments arranged by size:
6658 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4888 6494 7582 9270 9355 10426 4888
Size: 1606 1088 1688 85 1071 5671
Fragments arranged by size:
5671 1688 1606 1088 1071 85
BsmI GAATG_Cn'
Cuts at: 2260 3314 4479 7749 8720 8813 2260
Size: 1054 1165 3270 971 93 4656
Fragments arranged by size:
4656 3270 1165 1054 971 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4585 6114 4585
Size: 1529 9680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4071 4888 5852 6494 7582 7692 8528
Size: 399 2281 817 964 642 1088 110 836
Cuts at: 8528 9270 9355 10426 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2281 2174 1088 1071 964 836 817 742
642 399 110 85
BspEI T'CCGG_A
Cuts at: 10872 10872
Size: 11209
BspHI T'CATG_A
Cuts at: 2443 8239 9098 10828 2443
Size: 5796 859 1730 2824
Fragments arranged by size:
5796 2824 1730 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4156 7090 8564 844
Size: 3312 2934 1474 3489
Fragments arranged by size:
3489 3312 2934 1474
BsrBI CCG'CTC
Cuts at: 1394 1823 5089 7648 9096 1394
Size: 429 3266 2559 1448 3507
Fragments arranged by size:
3507 3266 2559 1448 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2724 3570 4530 4630 6116 6565 7386
Size: 1183 1476 846 960 100 1486 449 821
Cuts at: 7386 7938 9704 9878 65
Size: 552 1766 174 1396
Fragments arranged by size:
1766 1486 1476 1396 1183 960 846 821
552 449 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 6156 9853 10040 1700
Size: 110 4346 3697 187 2869
Fragments arranged by size:
4346 3697 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11209
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11209
BssSI C'ACGA_G
Cuts at: 9269 10281 9269
Size: 1012 10197
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4322 4474 794
Size: 3528 152 7529
Fragments arranged by size:
7529 3528 152
BstBI TT'CG_AA
Cuts at: 10013 10013
Size: 11209
BstEII G'GTnAC_C
Cuts at: 1184 2022 6017 1184
Size: 838 3995 6376
Fragments arranged by size:
6376 3995 838
BstXI CCAn_nnnn'nTGG
Cuts at: 3488 4945 6922 3488
Size: 1457 1977 7775
Fragments arranged by size:
7775 1977 1457
BstYI r'GATC_y
Cuts at: 1972 3287 6349 6812 7417 8539 8906 9291 9308
Size: 1315 3062 463 605 1122 367 385 17
Cuts at: 9308 10760 10846 10858 11204 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3062 1977 1452 1315 1122 605 463 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3045 3045
Size: 11209
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 9130
Fragments arranged by size:
9130 939 588 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10470
Fragments arranged by size:
10470 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 4484 7582 7732 8725 9519 9539 2797
Size: 1687 3098 150 993 794 20 4467
Fragments arranged by size:
4467 3098 1687 993 794 150 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2098 2128 2133 2163 529
Size: 35 1534 30 5 30 9575
Fragments arranged by size:
9575 1534 35 30 30 5
DraI TTT'AAA
Cuts at: 6312 8513 6312
Size: 2201 9008
DraIII CAC_nnn'GTG
Cuts at: 6707 6707
Size: 11209
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6763 8005 10216 809
Size: 507 5447 1242 2211 1802
Fragments arranged by size:
5447 2211 1802 1242 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6136 8452 8656
Size: 54 1425 50 275 18 4306 2316 204
Cuts at: 8656 9545 11174 8
Size: 889 1629 43
Fragments arranged by size:
4306 2316 1629 1425 889 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8656 1537
Size: 275 6844 4090
Fragments arranged by size:
6844 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3344 3831 4210 4491 5826 6033 6867 7592
Size: 1991 487 379 281 1335 207 834 725
Cuts at: 7592 7665 9139 1353
Size: 73 1474 3423
Fragments arranged by size:
3423 1991 1474 1335 834 725 487 379
281 207 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9796 10170 10316 387
Size: 1064 138 8207 374 146 1280
Fragments arranged by size:
8207 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4886 4886
Size: 11209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2067 2133 3276 3377 3837 4014
Size: 393 402 33 66 1143 101 460 177
Cuts at: 4014 4377 4402 5607 5615 5622 5793 5844 6368
Size: 363 25 1205 8 7 171 51 524
Cuts at: 6368 6848 6881 7029 7056 7668 8067 8148 9272
Size: 480 33 148 27 612 399 81 1124
Cuts at: 9272 9869 10656 10924 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1205 1143 1124 787 612 597 524
480 460 402 399 393 363 268 177
171 148 101 81 66 51 33 33
27 25 8 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3359 6963 7406 3359
Size: 3604 443 7162
Fragments arranged by size:
7162 3604 443
EcoO109I rG'GnC_Cy
Cuts at: 7180 8197 7180
Size: 1017 10192
EcoRI G'AATT_C
Cuts at: 2409 4631 8518 8896 2409
Size: 2222 3887 378 4722
Fragments arranged by size:
4722 3887 2222 378
EcoRV GAT'ATC
Cuts at: 4396 5995 4396
Size: 1599 9610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2120 5015 5435 5823 7856 8178 2120
Size: 2895 420 388 2033 322 5151
Fragments arranged by size:
5151 2895 2033 420 388 322
FspI TGC'GCA
Cuts at: 9715 9715
Size: 11209
HaeII r_GCGC'y
Cuts at: 1678 4300 10356 1678
Size: 2622 6056 2531
Fragments arranged by size:
6056 2622 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3869 3884 3901 3916 4579
Size: 32 135 32 2297 15 17 15 663
Cuts at: 4579 4611 5241 5273 5702 5734 7346 7378 7466
Size: 32 630 32 429 32 1612 32 88
Cuts at: 7466 7498 8042 8048 8074 8080 9850 9882 9924
Size: 32 544 6 26 6 1770 32 42
Cuts at: 9924 9956 1373
Size: 32 2626
Fragments arranged by size:
2626 2297 1770 1612 663 630 544 429
135 88 42 32 32 32 32 32
32 32 32 32 26 17 15 15
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2683 4607 6543 6741 8553 8799
Size: 988 281 745 1924 1936 198 1812 246
Cuts at: 8799 669
Size: 3079
Fragments arranged by size:
3079 1936 1924 1812 988 745 281 246
198
HindIII A'AGCT_T
Cuts at: 748 4709 5604 5634 6416 7188 748
Size: 3961 895 30 782 772 4769
Fragments arranged by size:
4769 3961 895 782 772 30
HpaI GTT'AAC
Cuts at: 8799 8799
Size: 11209
KasI G'GCGC_C
Cuts at: 4296 4296
Size: 11209
KpnI G_GTAC'C
Cuts at: 8534 8534
Size: 11209
MfeI C'AATT_G
Cuts at: 8808 8808
Size: 11209
MluI A'CGCG_T
Cuts at: 11085 11085
Size: 11209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4577 7442 10323 10507 1305
Size: 3272 2865 2881 184 2007
Fragments arranged by size:
3272 2881 2865 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6138 8454 10
Size: 54 1425 343 4306 2316 2765
Fragments arranged by size:
4306 2765 2316 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3120 5291 6920 8244 9167 9526 9685
Size: 648 1954 2171 1629 1324 923 359 159
Cuts at: 9685 518
Size: 2042
Fragments arranged by size:
2171 2042 1954 1629 1324 923 648 359
159
MspA1I CmG'CkG
Cuts at: 1606 2168 5438 7088 8625 9302 10450 10695 1606
Size: 562 3270 1650 1537 677 1148 245 2120
Fragments arranged by size:
3270 2120 1650 1537 1148 677 562 245
NarI GG'CG_CC
Cuts at: 4297 4297
Size: 11209
NcoI C'CATG_G
Cuts at: 513 1065 2004 2592 513
Size: 552 939 588 9130
Fragments arranged by size:
9130 939 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11209
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11209
NotI GC'GGCC_GC
Cuts at: 8656 8656
Size: 11209
NsiI A_TGCA'T
Cuts at: 8251 8251
Size: 11209
NspI r_CATG'y
Cuts at: 1132 1229 5203 5673 7586 8567 10112 1132
Size: 97 3974 470 1913 981 1545 2229
Fragments arranged by size:
3974 2229 1913 1545 981 470 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11209
PciI A'CATG_T
Cuts at: 5199 10108 5199
Size: 4909 6300
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2234 2668 4196 4619 6476 1108
Size: 1126 434 1528 423 1857 5841
Fragments arranged by size:
5841 1857 1528 1126 434 423
PfoI T'CCnGG_A
Cuts at: 4583 5245 5860 4583
Size: 662 615 9932
Fragments arranged by size:
9932 662 615
PmeI GTTT'AAAC
Cuts at: 8513 8513
Size: 11209
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9278 9310 10791 10823 9278
Size: 32 1481 32 9664
Fragments arranged by size:
9664 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 7180 8197 7180
Size: 1017 10192
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11209
PsiI TTA'TAA
Cuts at: 8295 8779 8295
Size: 484 10725
PstI C_TGCA'G
Cuts at: 830 7087 8561 830
Size: 6257 1474 3478
Fragments arranged by size:
6257 3478 1474
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11209
PvuII CAG'CTG
Cuts at: 2168 5438 7088 2168
Size: 3270 1650 6289
Fragments arranged by size:
6289 3270 1650
SacI G_AGCT'C
Cuts at: 4888 4888
Size: 11209
SalI G'TCGA_C
Cuts at: 1936 4605 8551 1936
Size: 2669 3946 4594
Fragments arranged by size:
4594 3946 2669
SapI GCTCTTCn'nnn_
Cuts at: 3344 5826 6033 3344
Size: 2482 207 8520
Fragments arranged by size:
8520 2482 207
SbfI CC_TGCA'GG
Cuts at: 8561 8561
Size: 11209
ScaI AGT'ACT
Cuts at: 9457 11169 9457
Size: 1712 9497
SexAI A'CCwGG_T
Cuts at: 2901 2901
Size: 11209
SfcI C'TryA_G
Cuts at: 826 1045 6622 7083 8557 9692 10373 10564 826
Size: 219 5577 461 1474 1135 681 191 1471
Fragments arranged by size:
5577 1474 1471 1135 681 461 219 191
SfoI GGC'GCC
Cuts at: 4298 4298
Size: 11209
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11209
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11209
SmaI CCC'GGG
Cuts at: 1872 8536 1872
Size: 6664 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2770 4399 4677 5725 7272 7283
Size: 197 926 827 1629 278 1048 1547 11
Cuts at: 7283 7341 8102 9313 10214 10476 820
Size: 58 761 1211 901 262 1553
Fragments arranged by size:
1629 1553 1547 1211 1048 926 901 827
761 278 262 197 58 11
SnaBI TAC'GTA
Cuts at: 493 9000 493
Size: 8507 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11209
SphI G_CATG'C
Cuts at: 1132 5673 8567 1132
Size: 4541 2894 3774
Fragments arranged by size:
4541 3774 2894
SspI AAT'ATT
Cuts at: 5 52 5341 6937 9133 5
Size: 47 5289 1596 2196 2081
Fragments arranged by size:
5289 2196 2081 1596 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 11209
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 2705 5844 5932 8193 8648
Size: 552 939 588 113 3139 88 2261 455
Cuts at: 8648 513
Size: 3074
Fragments arranged by size:
3139 3074 2261 939 588 552 455 113
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2241 2311 3456 4427 5390 5731 5869
Size: 1255 661 70 1145 971 963 341 138
Cuts at: 5869 6477 9236 9253 9406 9591 10036 325
Size: 608 2759 17 153 185 445 1498
Fragments arranged by size:
2759 1498 1255 1145 971 963 661 608
445 341 185 153 138 70 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2566 2609 3617
Size: 274 80 33 51 1443 589 43 1008
Cuts at: 3617 5267 5407 6084 9455 11167 96
Size: 1650 140 677 3371 1712 138
Fragments arranged by size:
3371 1712 1650 1443 1008 677 589 274
140 138 80 51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5842 5874 11169 11201 5842
Size: 32 5295 32 5850
Fragments arranged by size:
5850 5295 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11209
XbaI T'CTAG_A
Cuts at: 2806 4897 7420 8383 8545 2806
Size: 2091 2523 963 162 5470
Fragments arranged by size:
5470 2523 2091 963 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2343 7917 2231
Size: 112 5574 5523
Fragments arranged by size:
5574 5523 112
XhoI C'TCGA_G
Cuts at: 1943 7341 1943
Size: 5398 5811
XmaI C'CCGG_G
Cuts at: 1870 8534 1870
Size: 6664 4545
XmnI GAAnn'nnTTC
Cuts at: 3787 6520 7839 9338 3787
Size: 2733 1319 1499 5658
Fragments arranged by size:
5658 2733 1499 1319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 9017 276
Size: 53 83 186 936 3061 4422 2468
Fragments arranged by size:
4422 3061 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI
BstZ17I FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI
SwaI