Restriction map for
pFN21AE2406
(Circular) (Six-base) MAPSORT of: pFN21AE2406.seq Check: 3584 from: 1 to: 9196
pFN21AE2406 9196 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2269 3070 2269
Size: 801 8395
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7006 278
Size: 53 83 186 936 5470 2468
Fragments arranged by size:
5470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6539 1937
Size: 4602 4594
Acc65I G'GTAC_C
Cuts at: 4376 6517 4376
Size: 2141 7055
AclI AA'CG_TT
Cuts at: 1563 7323 7696 1563
Size: 5760 373 3063
Fragments arranged by size:
5760 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2061 3254 3509 4835 4968 5277 7259 8643
Size: 429 1193 255 1326 133 309 1982 1384
Cuts at: 8643 8911 1632
Size: 268 1917
Fragments arranged by size:
1982 1917 1384 1326 1193 429 309 268
255 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8999
AflIII A'CryG_T
Cuts at: 1203 4974 5415 8095 9072 1203
Size: 3771 441 2680 977 1327
Fragments arranged by size:
3771 2680 1327 977 441
AgeI A'CCGG_T
Cuts at: 8027 8027
Size: 9196
AhdI GACnn_n'nnGTC
Cuts at: 5608 7925 5608
Size: 2317 6879
AleI CACnn'nnGTG
Cuts at: 1166 2996 1166
Size: 1830 7366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 9164
AlwNI CAG_nnn'CTG
Cuts at: 3992 4046 4523 5141 6939 8511 3992
Size: 54 477 618 1798 1572 4677
Fragments arranged by size:
4677 1798 1572 618 477 54
ApaI G_GGCC'C
Cuts at: 3789 4037 4995 5004 5324 5463 5688 6203 6374
Size: 248 958 9 320 139 225 515 171
Cuts at: 6374 3789
Size: 6611
Fragments arranged by size:
6611 958 515 320 248 225 171 139
9
ApaLI G'TGCA_C
Cuts at: 7253 8409 7253
Size: 1156 8040
ApoI r'AATT_y
Cuts at: 1493 2378 2427 2536 5519 6505 6731 6883 8992
Size: 885 49 109 2983 986 226 152 2109
Cuts at: 8992 1493
Size: 1697
Fragments arranged by size:
2983 2109 1697 986 885 226 152 109
49
AseI AT'TA_AT
Cuts at: 160 1032 7750 160
Size: 872 6718 1606
Fragments arranged by size:
6718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9196
AvaI C'yCGr_G
Cuts at: 1870 1943 4155 4211 4638 5325 5455 5551 5679
Size: 73 2212 56 427 687 130 96 128
Cuts at: 5679 5689 6411 6521 1870
Size: 10 722 110 4545
Fragments arranged by size:
4545 2212 722 687 427 130 128 110
96 73 56 10
BamHI G'GATC_C
Cuts at: 5565 6526 6893 5565
Size: 961 367 7868
Fragments arranged by size:
7868 961 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 2894 3369 3586 4376
Size: 325 217 627 831 276 475 217 790
Cuts at: 4376 4511 5978 6517 7972 618
Size: 135 1467 539 1455 1842
Fragments arranged by size:
1842 1467 1455 831 790 627 539 475
325 276 217 217 135
BanII G_rGCy'C
Cuts at: 1391 2254 2342 2528 2603 2732 3648 3789 4037
Size: 863 88 186 75 129 916 141 248
Cuts at: 4037 4065 4212 4417 4924 4995 5004 5021 5324
Size: 28 147 205 507 71 9 17 303
Cuts at: 5324 5463 5496 5688 5707 6203 6374 6515 1391
Size: 139 33 192 19 496 171 141 4072
Fragments arranged by size:
4072 916 863 507 496 303 248 205
192 186 171 147 141 141 139 129
88 75 71 33 28 19 17 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2764 3165 3242 5265 6241 928
Size: 971 865 401 77 2023 976 3883
Fragments arranged by size:
3883 2023 976 971 865 401 77
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6007 6041 7387 7421 1903
Size: 34 4070 34 1346 34 3678
Fragments arranged by size:
4070 3678 1346 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 7088 8298 3788
Size: 3300 1210 4686
Fragments arranged by size:
4686 3300 1210
BclI T'GATC_A
Cuts at: 1548 5754 1548
Size: 4206 4990
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3378 3579 3864
Size: 107 122 71 631 605 1706 201 285
Cuts at: 3864 4032 4167 4527 4590 4609 4674 4728 4737
Size: 168 135 360 63 19 65 54 9
Cuts at: 4737 4872 4999 5008 5683 5805 6207 6226 7807
Size: 135 127 9 675 122 402 19 1581
Cuts at: 7807 136
Size: 1525
Fragments arranged by size:
1706 1581 1525 675 631 605 402 360
285 201 168 135 135 127 122 122
107 71 65 63 54 19 19 9
9
BglII A'GATC_T
Cuts at: 5087 6112 9191 5087
Size: 1025 3079 5092
Fragments arranged by size:
5092 3079 1025
BlpI GC'TnA_GC
Cuts at: 6613 6613
Size: 9196
Bme1580I G_kGCm'C
Cuts at: 1790 3374 3789 4037 4516 4995 5004 5324 5463
Size: 1584 415 248 479 479 9 320 139
Cuts at: 5463 5688 6203 6347 6374 7257 8413 1790
Size: 225 515 144 27 883 1156 2573
Fragments arranged by size:
2573 1584 1156 883 515 479 479 415
320 248 225 144 139 27 9
BmgBI CAC'GTC
Cuts at: 1318 5290 1318
Size: 3972 5224
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2599 4293 6927
Size: 416 53 83 399 24 1182 1694 2634
Cuts at: 6927 7885 442
Size: 958 1753
Fragments arranged by size:
2634 1753 1694 1182 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5515 5623 5515
Size: 108 9088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2326 3530 3560 3756 3945 4023 4341
Size: 941 146 1204 30 196 189 78 318
Cuts at: 4341 4401 4491 4503 5637 6129 6333 7856 1239
Size: 60 90 12 1134 492 204 1523 2579
Fragments arranged by size:
2579 1523 1204 1134 941 492 318 204
196 189 146 90 78 60 30 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2483 7321 8186 8484 2483
Size: 4838 865 298 3195
Fragments arranged by size:
4838 3195 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2573 3222 4058 4317 4346 4544 4752
Size: 617 1074 649 836 259 29 198 208
Cuts at: 4752 7859 882
Size: 3107 2219
Fragments arranged by size:
3107 2219 1074 836 649 617 259 208
198 29
BsaAI yAC'GTr
Cuts at: 493 1204 4598 4975 6987 493
Size: 711 3394 377 2012 2702
Fragments arranged by size:
3394 2702 2012 711 377
BsaBI GATnn'nnATC
Cuts at: 9190 9190
Size: 9196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2795 7003 7385 8020
Size: 53 83 186 936 1262 4208 382 635
Cuts at: 8020 275
Size: 1451
Fragments arranged by size:
4208 1451 1262 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2359 5099 5742 7629 8027 8301 8448 8859 2359
Size: 2740 643 1887 398 274 147 411 2696
Fragments arranged by size:
2740 2696 1887 643 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2329 2359 2639 2669 6463
Size: 30 165 30 926 30 280 30 3794
Cuts at: 6463 6493 1178
Size: 30 3881
Fragments arranged by size:
3881 3794 926 280 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2729 2827 3327 3488 3538 3656 5486 5504
Size: 1543 98 500 161 50 118 1830 18
Cuts at: 5504 5875 5878 5961 1186
Size: 371 3 83 4421
Fragments arranged by size:
4421 1830 1543 500 371 161 118 98
83 50 18 3
BseYI C'CCAG_C
Cuts at: 2870 3409 4531 6377 6458 8399 8895 2870
Size: 539 1122 1846 81 1941 496 3171
Fragments arranged by size:
3171 1941 1846 1122 539 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2971 3020 3608 5976 5991 1163
Size: 573 1235 49 588 2368 15 4368
Fragments arranged by size:
4368 2368 1235 588 573 49 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2513 6646 7407 8435 1540
Size: 275 186 512 4133 761 1028 2301
Fragments arranged by size:
4133 2301 1028 761 512 275 186
BsiHKAI G_wGCw'C
Cuts at: 2342 2603 2732 3648 4417 5496 5942 7257 7342
Size: 261 129 916 769 1079 446 1315 85
Cuts at: 7342 8413 2342
Size: 1071 3125
Fragments arranged by size:
3125 1315 1079 1071 916 769 446 261
129 85
BsmI GAATG_Cn'
Cuts at: 4929 5403 6707 6800 4929
Size: 474 1304 93 7325
Fragments arranged by size:
7325 1304 474 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2254 2342 2528 2603 2732 3374 3648
Size: 399 464 88 186 75 129 642 274
Cuts at: 3648 3789 4037 4065 4212 4417 4516 4924 4995
Size: 141 248 28 147 205 99 408 71
Cuts at: 4995 5004 5021 5324 5463 5496 5688 5707 5942
Size: 9 17 303 139 33 192 19 235
Cuts at: 5942 6203 6347 6374 6515 7257 7342 8413 1391
Size: 261 144 27 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 642 464 408 399 303
274 261 248 235 205 192 186 147
144 141 141 139 129 99 88 85
75 71 33 28 27 19 17 9
BspEI T'CCGG_A
Cuts at: 2359 5099 5742 8859 2359
Size: 2740 643 3117 2696
Fragments arranged by size:
3117 2740 2696 643
BspHI T'CATG_A
Cuts at: 3090 7085 8815 3090
Size: 3995 1730 3471
Fragments arranged by size:
3995 3471 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2269 3070 6551 844
Size: 1425 801 3481 3489
Fragments arranged by size:
3489 3481 1425 801
BsrBI CCG'CTC
Cuts at: 1394 1823 7083 1394
Size: 429 5260 3507
Fragments arranged by size:
5260 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 2637 3459 4233 5581 6085 7691
Size: 1183 874 515 822 774 1348 504 1606
Cuts at: 7691 7865 65
Size: 174 1396
Fragments arranged by size:
1606 1396 1348 1183 874 822 774 515
504 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2154 4526 5627 6493 7840 8027 1700
Size: 110 344 2372 1101 866 1347 187 2869
Fragments arranged by size:
2869 2372 1347 1101 866 344 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9196
BssSI C'ACGA_G
Cuts at: 2217 2415 3897 7256 8268 2217
Size: 198 1482 3359 1012 3145
Fragments arranged by size:
3359 3145 1482 1012 198
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5717 5978 794
Size: 4923 261 4012
Fragments arranged by size:
4923 4012 261
BstBI TT'CG_AA
Cuts at: 8000 8000
Size: 9196
BstEII G'GTnAC_C
Cuts at: 1184 4133 6443 1184
Size: 2949 2310 3937
Fragments arranged by size:
3937 2949 2310
BstXI CCAn_nnnn'nTGG
Cuts at: 6302 6302
Size: 9196
BstYI r'GATC_y
Cuts at: 1972 3841 3920 5087 5565 6112 6526 6893 7278
Size: 1869 79 1167 478 547 414 367 385
Cuts at: 7278 7295 8747 8833 8845 9191 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1869 1452 1167 547 478 414 385
367 346 86 79 17 12
Bsu36I CC'TnA_GG
Cuts at: 4362 4822 4362
Size: 460 8736
BtgI C'CryG_G
Cuts at: 513 1065 2004 2682 2694 2890 3591 3861 3888
Size: 552 939 678 12 196 701 270 27
Cuts at: 3888 4300 4488 5853 513
Size: 412 188 1365 3856
Fragments arranged by size:
3856 1365 939 701 678 552 412 270
196 188 27 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3021 3121 487
Size: 683 56 1795 100 6562
Fragments arranged by size:
6562 1795 683 100 56
BtsI GCAGTG_nn'
Cuts at: 3264 3298 3313 6712 7506 7526 3264
Size: 34 15 3399 794 20 4934
Fragments arranged by size:
4934 3399 794 34 20 15
ClaI AT'CG_AT
Cuts at: 1554 4448 1554
Size: 2894 6302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9161
DraI TTT'AAA
Cuts at: 6500 6500
Size: 9196
DraIII CAC_nnn'GTG
Cuts at: 3980 5197 3980
Size: 1217 7979
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2884 3819 5544 5664 8203 809
Size: 507 1568 935 1725 120 2539 1802
Fragments arranged by size:
2539 1802 1725 1568 935 507 120
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4384 5035 6643
Size: 54 1425 50 275 18 2554 651 1608
Cuts at: 6643 7532 9161 8
Size: 889 1629 43
Fragments arranged by size:
2554 1629 1608 1425 889 651 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6643 1537
Size: 275 4831 4090
Fragments arranged by size:
4831 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 3455 5257 5935 7126 1353
Size: 1006 1096 1802 678 1191 3423
Fragments arranged by size:
3423 1802 1191 1096 1006 678
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2968 3485 5322 6051 6099 6478
Size: 1064 138 1379 517 1837 729 48 379
Cuts at: 6478 7783 8157 8303 387
Size: 1305 374 146 1280
Fragments arranged by size:
1837 1379 1305 1280 1064 729 517 379
374 146 138 48
EcoICRI GAG'CTC
Cuts at: 2340 2601 2730 3646 4415 5494 2340
Size: 261 129 916 769 1079 6042
Fragments arranged by size:
6042 1079 916 769 261 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2180 2326 3254 3509 3530 3560
Size: 393 429 119 146 928 255 21 30
Cuts at: 3560 3756 3945 4023 4341 4401 4491 4503 4835
Size: 196 189 78 318 60 90 12 332
Cuts at: 4835 4968 5277 5637 6129 6333 7259 7856 8643
Size: 133 309 360 492 204 926 597 787
Cuts at: 8643 8911 1239
Size: 268 1524
Fragments arranged by size:
1524 928 926 787 597 492 429 393
360 332 318 309 268 255 204 196
189 146 133 119 90 78 60 30
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2299 4947 2299
Size: 2648 6548
EcoO109I rG'GnC_Cy
Cuts at: 2688 2700 3740 3786 3857 3884 3893 3911 3962
Size: 12 1040 46 71 27 9 18 51
Cuts at: 3962 3998 4033 4034 4043 4124 4151 4232 4313
Size: 36 35 1 9 81 27 81 81
Cuts at: 4313 4349 4358 4466 4475 4493 4502 4520 4574
Size: 36 9 108 9 18 9 18 54
Cuts at: 4574 4610 4634 4748 4766 4775 4991 4992 5001
Size: 36 24 114 18 9 216 1 9
Cuts at: 5001 5111 5138 5321 5364 5459 5460 5546 5602
Size: 110 27 183 43 95 1 86 56
Cuts at: 5602 5648 5657 5675 5684 5685 5807 5825 6008
Size: 46 9 18 9 1 122 18 183
Cuts at: 6008 6166 6199 6317 6353 6370 6371 2688
Size: 158 33 118 36 17 1 5513
Fragments arranged by size:
5513 1040 216 183 183 158 122 118
114 110 108 95 86 81 81 81
71 56 54 51 46 46 43 36
36 36 36 35 33 27 27 27
24 18 18 18 18 18 18 17
12 9 9 9 9 9 9 9
9 9 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 2536 5519 6505 6883 2536
Size: 2983 986 378 4849
Fragments arranged by size:
4849 2983 986 378
EcoRV GAT'ATC
Cuts at: 3274 3274
Size: 9196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4097 4577 4097
Size: 480 8716
FspI TGC'GCA
Cuts at: 5783 7702 5783
Size: 1919 7277
HaeII r_GCGC'y
Cuts at: 1678 3336 3362 8343 1678
Size: 1658 26 4981 2531
Fragments arranged by size:
4981 2531 1658 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4430 4462 5308 5340 5594
Size: 32 135 32 2858 32 846 32 254
Cuts at: 5594 5626 6331 6363 7837 7869 7911 7943 1373
Size: 32 705 32 1474 32 42 32 2626
Fragments arranged by size:
2858 2626 1474 846 705 254 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6540 6786 669
Size: 988 281 4602 246 3079
Fragments arranged by size:
4602 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9196
HpaI GTT'AAC
Cuts at: 6786 6786
Size: 9196
KpnI G_GTAC'C
Cuts at: 4380 6521 4380
Size: 2141 7055
MfeI C'AATT_G
Cuts at: 6795 6795
Size: 9196
MluI A'CGCG_T
Cuts at: 9072 9072
Size: 9196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2568 4722 5515 8310 8494 1305
Size: 1263 2154 793 2795 184 2007
Fragments arranged by size:
2795 2154 2007 1263 793 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5037 10
Size: 54 1425 343 3205 4169
Fragments arranged by size:
4169 3205 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2996 4973 5594 7154 7513 7672
Size: 648 1162 668 1977 621 1560 359 159
Cuts at: 7672 518
Size: 2042
Fragments arranged by size:
2042 1977 1560 1162 668 648 621 359
159
MspA1I CmG'CkG
Cuts at: 1606 2190 2684 3105 3393 3593 4014 4535 5281
Size: 584 494 421 288 200 421 521 746
Cuts at: 5281 5723 6612 7289 8437 8682 1606
Size: 442 889 677 1148 245 2120
Fragments arranged by size:
2120 1148 889 746 677 584 521 494
442 421 421 288 245 200
NaeI GCC'GGC
Cuts at: 2156 4528 5629 2156
Size: 2372 1101 5723
Fragments arranged by size:
5723 2372 1101
NcoI C'CATG_G
Cuts at: 513 1065 2004 2694 2890 3861 3888 4488 513
Size: 552 939 690 196 971 27 600 5221
Fragments arranged by size:
5221 971 939 690 600 552 196 27
NdeI CA'TA_TG
Cuts at: 387 2917 387
Size: 2530 6666
NgoMIV G'CCGG_C
Cuts at: 2154 4526 5627 2154
Size: 2372 1101 5723
Fragments arranged by size:
5723 2372 1101
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9196
NotI GC'GGCC_GC
Cuts at: 6643 6643
Size: 9196
NspI r_CATG'y
Cuts at: 1132 1229 2854 2938 5071 5419 6070 6157 6554
Size: 97 1625 84 2133 348 651 87 397
Cuts at: 6554 8099 1132
Size: 1545 2229
Fragments arranged by size:
2229 2133 1625 1545 651 397 348 97
87 84
PasI CC'CwG_GG
Cuts at: 1348 3764 3782 4354 4879 4997 5006 5672 5831
Size: 2416 18 572 525 118 9 666 159
Cuts at: 5831 5965 6323 1348
Size: 134 358 4221
Fragments arranged by size:
4221 2416 666 572 525 358 159 134
118 18 9
PciI A'CATG_T
Cuts at: 5415 8095 5415
Size: 2680 6516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2466 3583 1108
Size: 1358 1117 6721
Fragments arranged by size:
6721 1358 1117
PfoI T'CCnGG_A
Cuts at: 2637 3916 4480 5653 6349 2637
Size: 1279 564 1173 696 5484
Fragments arranged by size:
5484 1279 1173 696 564
PmeI GTTT'AAAC
Cuts at: 6500 6500
Size: 9196
PmlI CAC'GTG
Cuts at: 4598 4598
Size: 9196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7265 7297 8778 8810 7265
Size: 32 1481 32 7651
Fragments arranged by size:
7651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2688 2700 3893 3962 3998 4043 4124 4151 4232
Size: 12 1193 69 36 45 81 27 81
Cuts at: 4232 4313 4349 4358 4475 4493 4502 4574 4634
Size: 81 36 9 117 18 9 72 60
Cuts at: 4634 4748 4766 5111 5138 5364 5546 5602 5657
Size: 114 18 345 27 226 182 56 55
Cuts at: 5657 5825 6008 6317 6353 2688
Size: 168 183 309 36 5531
Fragments arranged by size:
5531 1193 345 309 226 183 182 168
117 114 81 81 81 72 69 60
56 55 45 36 36 36 27 27
18 18 12 9 9
PshAI GACnn'nnGTC
Cuts at: 1613 4764 5292 6006 1613
Size: 3151 528 714 4803
Fragments arranged by size:
4803 3151 714 528
PsiI TTA'TAA
Cuts at: 6766 6766
Size: 9196
PspOMI G'GGCC_C
Cuts at: 3785 4033 4991 5000 5320 5459 5684 6199 6370
Size: 248 958 9 320 139 225 515 171
Cuts at: 6370 3785
Size: 6611
Fragments arranged by size:
6611 958 515 320 248 225 171 139
9
PspXI vC'TCGA_Gb
Cuts at: 4155 5679 6411 4155
Size: 1524 732 6940
Fragments arranged by size:
6940 1524 732
PstI C_TGCA'G
Cuts at: 830 2445 3271 3392 5280 5998 6548 830
Size: 1615 826 121 1888 718 550 3478
Fragments arranged by size:
3478 1888 1615 826 718 550 121
PvuI CG_AT'CG
Cuts at: 2001 2513 2001
Size: 512 8684
PvuII CAG'CTG
Cuts at: 3393 4535 5723 3393
Size: 1142 1188 6866
Fragments arranged by size:
6866 1188 1142
SacI G_AGCT'C
Cuts at: 2342 2603 2732 3648 4417 5496 2342
Size: 261 129 916 769 1079 6042
Fragments arranged by size:
6042 1079 916 769 261 129
SacII CC_GC'GG
Cuts at: 2685 3594 2685
Size: 909 8287
SalI G'TCGA_C
Cuts at: 1936 6538 1936
Size: 4602 4594
SanDI GG'GwC_CC
Cuts at: 3998 4493 5111 5364 5825 6008 3998
Size: 495 618 253 461 183 7186
Fragments arranged by size:
7186 618 495 461 253 183
SapI GCTCTTCn'nnn_
Cuts at: 3455 5935 3455
Size: 2480 6716
SbfI CC_TGCA'GG
Cuts at: 6548 6548
Size: 9196
ScaI AGT'ACT
Cuts at: 5312 7444 9156 5312
Size: 2132 1712 5352
Fragments arranged by size:
5352 2132 1712
SexAI A'CCwGG_T
Cuts at: 3958 3976 5125 6385 3958
Size: 18 1149 1260 6769
Fragments arranged by size:
6769 1260 1149 18
SfcI C'TryA_G
Cuts at: 826 1045 2441 3267 3283 3388 4047 5276 5923
Size: 219 1396 826 16 105 659 1229 647
Cuts at: 5923 5994 6259 6544 7679 8360 8551 826
Size: 71 265 285 1135 681 191 1471
Fragments arranged by size:
1471 1396 1229 1135 826 681 659 647
285 265 219 191 105 71 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 3864 4032 4527 4590 4609 4674 4728 4737 4872
Size: 168 495 63 19 65 54 9 135
Cuts at: 4872 4999 5008 5683 5805 6207 6226 3864
Size: 127 9 675 122 402 19 6834
Fragments arranged by size:
6834 675 495 402 168 135 127 122
65 63 54 19 19 9 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9196
SmaI CCC'GGG
Cuts at: 1872 4213 5457 5553 5691 6523 1872
Size: 2341 1244 96 138 832 4545
Fragments arranged by size:
4545 2341 1244 832 138 96
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 4155 5679 6411 7300 8201
Size: 197 926 555 1657 1524 732 889 901
Cuts at: 8201 8463 820
Size: 262 1553
Fragments arranged by size:
1657 1553 1524 926 901 889 732 555
262 197
SnaBI TAC'GTA
Cuts at: 493 6987 493
Size: 6494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9196
SphI G_CATG'C
Cuts at: 1132 2854 6157 6554 1132
Size: 1722 3303 397 3774
Fragments arranged by size:
3774 3303 1722 397
SspI AAT'ATT
Cuts at: 5 52 7120 5
Size: 47 7068 2081
Fragments arranged by size:
7068 2081 47
StuI AGG'CCT
Cuts at: 1517 3207 4161 4803 1517
Size: 1690 954 642 5910
Fragments arranged by size:
5910 1690 954 642
StyI C'CwwG_G
Cuts at: 513 1065 2004 2694 2890 3096 3347 3789 3861
Size: 552 939 690 196 206 251 442 72
Cuts at: 3861 3888 4057 4255 4488 4752 4762 4789 6635
Size: 27 169 198 233 264 10 27 1846
Cuts at: 6635 513
Size: 3074
Fragments arranged by size:
3074 1846 939 690 552 442 264 251
233 206 198 196 169 72 27 27
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2821 3813 5957 7223 7240 7393 7578
Size: 1255 1241 992 2144 1266 17 153 185
Cuts at: 7578 8023 325
Size: 445 1498
Fragments arranged by size:
2144 1498 1266 1255 1241 992 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5052 5064 5310
Size: 274 80 33 51 1443 3075 12 246
Cuts at: 5310 7442 9154 96
Size: 2132 1712 138
Fragments arranged by size:
3075 2132 1712 1443 274 246 138 80
51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2329 2361 2541 2573 3966 3998 5550 5582 9156
Size: 32 180 32 1393 32 1552 32 3574
Cuts at: 9156 9188 2329
Size: 32 2337
Fragments arranged by size:
3574 2337 1552 1393 180 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2406 3241 6449 1316
Size: 1090 835 3208 4063
Fragments arranged by size:
4063 3208 1090 835
XbaI T'CTAG_A
Cuts at: 6532 6532
Size: 9196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2463 3073 3363 5577 5593 6455 2463
Size: 610 290 2214 16 862 5204
Fragments arranged by size:
5204 2214 862 610 290 16
XhoI C'TCGA_G
Cuts at: 1943 4155 5679 6411 1943
Size: 2212 1524 732 4728
Fragments arranged by size:
4728 2212 1524 732
XmaI C'CCGG_G
Cuts at: 1870 4211 5455 5551 5689 6521 1870
Size: 2341 1244 96 138 832 4545
Fragments arranged by size:
4545 2341 1244 832 138 96
XmnI GAAnn'nnTTC
Cuts at: 7325 7325
Size: 9196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7004 276
Size: 53 83 186 936 5470 2468
Fragments arranged by size:
5470 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI
NsiI PacI PsrI RsrII SfoI SrfI SwaI