Restriction map for
pFN21AE2404
(Circular) (Six-base) MAPSORT of: pFN21AE2404.seq Check: 9326 from: 1 to: 5788
pFN21AE2404 5788 bp
With 182 enzymes: SgfI *
February 28, 2012 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2029 2029
Size: 5788
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3056 3095 3851 5235 5503 1632
Size: 1424 39 756 1384 268 1917
Fragments arranged by size:
1917 1424 1384 756 268 39
AflII C'TTAA_G
Cuts at: 820 1017 2339 820
Size: 197 1322 4269
Fragments arranged by size:
4269 1322 197
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 2483
Size: 32 5756
AlwNI CAG_nnn'CTG
Cuts at: 2155 3531 5103 2155
Size: 1376 1572 2840
Fragments arranged by size:
2840 1572 1376
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2665 2828 3005 3097 3323 3475 5584 1493
Size: 1172 163 177 92 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 226 177 163 152 92
AseI AT'TA_AT
Cuts at: 160 1032 2676 4342 160
Size: 872 1644 1666 1606
Fragments arranged by size:
1666 1644 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 3113 1870
Size: 73 1170 4545
Fragments arranged by size:
4545 1170 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2872 3109 4564 618
Size: 325 217 627 1085 237 1455 1842
Fragments arranged by size:
1842 1455 1085 627 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2072 3107 1391
Size: 681 1035 4072
Fragments arranged by size:
4072 1035 681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BbvCI CC'TCA_GC
Cuts at: 2108 2624 2108
Size: 516 5272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 3849 5005 1790
Size: 2059 1156 2573
Fragments arranged by size:
2573 2059 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4448 1239
Size: 3209 2579
Bpu10I CC'TnA_GC
Cuts at: 2108 2624 2108
Size: 516 5272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 2330 3913 4778 5076 2065
Size: 265 1583 865 298 2777
Fragments arranged by size:
2777 1583 865 298 265
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 4451 882
Size: 617 672 2280 2219
Fragments arranged by size:
2280 2219 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 2620 2650 1178
Size: 30 165 30 1080 30 107 30 4316
Fragments arranged by size:
4316 1080 165 107 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 2155 2193 2201 2641 3057 1186
Size: 909 60 38 8 440 416 3917
Fragments arranged by size:
3917 909 440 416 60 38 8
BseYI C'CCAG_C
Cuts at: 2033 2453 4991 5487 2033
Size: 420 2538 496 2334
Fragments arranged by size:
2538 2334 496 420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 3849 3934 5005 2072
Size: 1777 85 1071 2855
Fragments arranged by size:
2855 1777 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 3107 3849 3934 5005 1391
Size: 399 282 1035 742 85 1071 2174
Fragments arranged by size:
2174 1071 1035 742 399 282 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 3143 844
Size: 1185 1114 3489
Fragments arranged by size:
3489 1185 1114
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2479 4283 4457 65
Size: 1183 1231 1804 174 1396
Fragments arranged by size:
1804 1396 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2510 2510
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783
Size: 1146 367 385 17 1452 86 12 346
Cuts at: 5783 1972
Size: 1977
Fragments arranged by size:
1977 1452 1146 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2581 513
Size: 552 1516 3720
Fragments arranged by size:
3720 1516 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 3304 4098 4118 3304
Size: 794 20 4974
Fragments arranged by size:
4974 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2561 4795 809
Size: 507 1245 2234 1802
Fragments arranged by size:
2234 1802 1245 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2578 2584 3235
Size: 54 1425 50 275 18 748 6 651
Cuts at: 3235 4124 5753 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 748 651 275 54 50
43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2688 3718 1353
Size: 834 501 1030 3423
Fragments arranged by size:
3423 1030 834 501
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2070 2070
Size: 5788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3056 3095 3851 4448 5235 5503 1239
Size: 393 1424 39 756 597 787 268 1524
Fragments arranged by size:
1524 1424 787 756 597 393 268 39
EcoO109I rG'GnC_Cy
Cuts at: 2197 2959 2197
Size: 762 5026
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 5788
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2851 4935 1678
Size: 1173 2084 2531
Fragments arranged by size:
2531 2084 1173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2230 3132 3378 669
Size: 988 281 292 902 246 3079
Fragments arranged by size:
3079 988 902 292 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4902 5086 1305
Size: 3597 184 2007
Fragments arranged by size:
3597 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2586 10
Size: 54 1425 343 754 3212
Fragments arranged by size:
3212 1425 754 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3746 4105 4264 518
Size: 648 843 1737 359 159 2042
Fragments arranged by size:
2042 1737 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2155 2736 3204 3881 5029 5274 1606
Size: 489 60 581 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 489 468 245 60
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5236
NdeI CA'TA_TG
Cuts at: 387 3056 387
Size: 2669 3119
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PfoI T'CCnGG_A
Cuts at: 2487 2976 2487
Size: 489 5299
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2483 2515 3857 3889 5370 5402 2483
Size: 32 1342 32 1481 32 2869
Fragments arranged by size:
2869 1481 1342 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2959 2197
Size: 762 5026
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 5788
SacI G_AGCT'C
Cuts at: 2072 2072
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2941 3136 4271 4952 5143 826
Size: 219 1896 195 1135 681 191 1471
Fragments arranged by size:
1896 1471 1135 681 219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2044 2309 2339 3892 4793 5055
Size: 197 926 101 265 30 1553 901 262
Cuts at: 5055 820
Size: 1553
Fragments arranged by size:
1553 1553 926 901 265 262 197 101
30
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2587 3227 513
Size: 552 1522 640 3074
Fragments arranged by size:
3074 1522 640 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1999 3815 3832 3985 4170 4615 325
Size: 1255 419 1816 17 153 185 445 1498
Fragments arranged by size:
1816 1498 1255 445 419 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2974 2974
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 2613 2744 3917 2613
Size: 131 1173 4484
Fragments arranged by size:
4484 1173 131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI