Restriction map for
pFN21AE2402
(Circular) (Six-base) MAPSORT of: pFN21AE2402.seq Check: 1782 from: 1 to: 6787
pFN21AE2402 6787 bp
With 182 enzymes: SgfI *
February 28, 2012 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 278
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3964 4130 1937
Size: 2027 166 4594
Fragments arranged by size:
4594 2027 166
Acc65I G'GTAC_C
Cuts at: 4108 4108
Size: 6787
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2402 2738 4850 6234 6502 1632
Size: 770 336 2112 1384 268 1917
Fragments arranged by size:
2112 1917 1384 770 336 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6590
AflIII A'CryG_T
Cuts at: 1203 5686 6663 1203
Size: 4483 977 1327
Fragments arranged by size:
4483 1327 977
AgeI A'CCGG_T
Cuts at: 5618 5618
Size: 6787
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 2261 1166
Size: 1095 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2253 1184
Size: 1069 5718
AlwNI CAG_nnn'CTG
Cuts at: 2953 3541 4530 6102 2953
Size: 588 989 1572 3638
Fragments arranged by size:
3638 1572 989 588
ApaI G_GGCC'C
Cuts at: 2043 2199 2597 2613 2637 2888 3144 3167 4038
Size: 156 398 16 24 251 256 23 871
Cuts at: 4038 2043
Size: 4792
Fragments arranged by size:
4792 871 398 256 251 156 24 23
16
ApaLI G'TGCA_C
Cuts at: 2233 3893 4844 6000 2233
Size: 1660 951 1156 3020
Fragments arranged by size:
3020 1660 1156 951
ApoI r'AATT_y
Cuts at: 1493 2321 4096 4322 4474 6583 1493
Size: 828 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 828 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5341 160
Size: 872 4309 1606
Fragments arranged by size:
4309 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2198 2770 2840 3075 3279 3311 4112
Size: 73 255 572 70 235 204 32 801
Cuts at: 4112 1870
Size: 4545
Fragments arranged by size:
4545 801 572 255 235 204 73 70
32
BamHI G'GATC_C
Cuts at: 3260 4117 4484 3260
Size: 857 367 5563
Fragments arranged by size:
5563 857 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2522 2712 2893 2962 4108
Size: 325 217 627 735 190 181 69 1146
Cuts at: 4108 5563 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1146 735 627 325 217 190
181 69
BanII G_rGCy'C
Cuts at: 1391 2043 2199 2597 2613 2637 2835 2888 3144
Size: 652 156 398 16 24 198 53 256
Cuts at: 3144 3167 3237 3587 4038 4106 1391
Size: 23 70 350 451 68 4072
Fragments arranged by size:
4072 652 451 398 350 256 198 156
70 68 53 24 23 16
BbeI G_GCGC'C
Cuts at: 2526 2526
Size: 6787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3292 928
Size: 971 1393 4423
Fragments arranged by size:
4423 1393 971
BbvCI CC'TCA_GC
Cuts at: 2397 3473 2397
Size: 1076 5711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2100 2133 2134 2167 2344 2378 3995
Size: 34 163 33 1 33 177 34 1617
Cuts at: 3995 4029 4978 5012 1903
Size: 34 949 34 3678
Fragments arranged by size:
3678 1617 949 177 163 34 34 34
34 33 33 1
BciVI GTATCCnnnnn_n'
Cuts at: 4679 5889 4679
Size: 1210 5577
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2608 4022 5398
Size: 107 122 71 631 605 936 1414 1376
Cuts at: 5398 136
Size: 1525
Fragments arranged by size:
1525 1414 1376 936 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6782 6782
Size: 6787
BlpI GC'TnA_GC
Cuts at: 3586 3800 4204 3586
Size: 214 404 6169
Fragments arranged by size:
6169 404 214
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2199 2237 2441 2597 2613 2637 2888
Size: 253 156 38 204 156 16 24 251
Cuts at: 2888 3020 3144 3167 3466 3897 4018 4038 4848
Size: 132 124 23 299 431 121 20 810
Cuts at: 4848 6004 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 810 431 299 253 251 204
156 156 132 124 121 38 24 23
20 16
BmgBI CAC'GTC
Cuts at: 1318 2626 3832 3870 1318
Size: 1308 1206 38 4235
Fragments arranged by size:
4235 1308 1206 38
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2857 3837 4518
Size: 416 53 83 399 24 1440 980 681
Cuts at: 4518 5476 442
Size: 958 1753
Fragments arranged by size:
1753 1440 980 958 681 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3215 5447 1239
Size: 1976 2232 2579
Fragments arranged by size:
2579 2232 1976
Bpu10I CC'TnA_GC
Cuts at: 2397 3231 3401 3473 3815 2397
Size: 834 170 72 342 5369
Fragments arranged by size:
5369 834 342 170 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4061 4912 5777 6075 4061
Size: 851 865 298 4773
Fragments arranged by size:
4773 865 851 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3878 5450 882
Size: 617 2379 1572 2219
Fragments arranged by size:
2379 2219 1572 617
BsaAI yAC'GTr
Cuts at: 493 1204 3205 4578 493
Size: 711 2001 1373 2702
Fragments arranged by size:
2702 2001 1373 711
BsaBI GATnn'nnATC
Cuts at: 6781 6781
Size: 6787
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 2107 2523 2736
Size: 53 83 186 936 524 50 416 213
Cuts at: 2736 4594 4976 5611 275
Size: 1858 382 635 1451
Fragments arranged by size:
1858 1451 936 635 524 416 382 213
186 83 53 50
BsaWI w'CCGG_w
Cuts at: 5220 5618 5892 6039 6450 5220
Size: 398 274 147 411 5557
Fragments arranged by size:
5557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2981 2987 3011 3017 3968
Size: 30 165 30 1578 6 24 6 951
Cuts at: 3968 3998 1178
Size: 30 3967
Fragments arranged by size:
3967 1578 951 165 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2531 2570 2735 2965 2974 3845 4076 1186
Size: 1345 39 165 230 9 871 231 3897
Fragments arranged by size:
3897 1345 871 231 230 165 39 9
BseYI C'CCAG_C
Cuts at: 2285 2430 2887 3166 3648 3795 5990 6486 2285
Size: 145 457 279 482 147 2195 496 2586
Fragments arranged by size:
2586 2195 496 482 457 279 147 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2431 1163
Size: 573 695 5519
Fragments arranged by size:
5519 695 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4237 4998 6026 1540
Size: 275 186 2236 761 1028 2301
Fragments arranged by size:
2301 2236 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2250 3549 3897 4848 4933 6004 2237
Size: 13 1299 348 951 85 1071 3020
Fragments arranged by size:
3020 1299 1071 951 348 85 13
BsmI GAATG_Cn'
Cuts at: 4298 4391 4298
Size: 93 6694
BsmBI CGTCTCn'nnnn_
Cuts at: 2727 3779 2727
Size: 1052 5735
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2199 2237 2250 2441 2597 2613
Size: 399 253 156 38 13 191 156 16
Cuts at: 2613 2637 2835 2888 3020 3144 3167 3237 3466
Size: 24 198 53 132 124 23 70 229
Cuts at: 3466 3549 3587 3897 4018 4038 4106 4848 4933
Size: 83 38 310 121 20 68 742 85
Cuts at: 4933 6004 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 253 229 198
191 156 156 132 124 121 85 83
70 68 53 38 38 24 23 20
16 13
BspEI T'CCGG_A
Cuts at: 6450 6450
Size: 6787
BspHI T'CATG_A
Cuts at: 4676 6406 4676
Size: 1730 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 3987 4142 844
Size: 2890 253 155 3489
Fragments arranged by size:
3489 2890 253 155
BsrBI CCG'CTC
Cuts at: 1394 1823 2538 3199 4674 1394
Size: 429 715 661 1475 3507
Fragments arranged by size:
3507 1475 715 661 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5282 5456 65
Size: 1183 4034 174 1396
Fragments arranged by size:
4034 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3405 5431 5618 1700
Size: 110 1595 2026 187 2869
Fragments arranged by size:
2869 2026 1595 187 110
BsrGI T'GTAC_A
Cuts at: 96 2345 96
Size: 2249 4538
BssHII G'CGCG_C
Cuts at: 1925 2099 1925
Size: 174 6613
BssSI C'ACGA_G
Cuts at: 4847 5859 4847
Size: 1012 5775
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3208 794
Size: 2414 4373
BstBI TT'CG_AA
Cuts at: 5591 5591
Size: 6787
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6787
BstYI r'GATC_y
Cuts at: 1972 2418 3260 4117 4484 4869 4886 6338 6424
Size: 446 842 857 367 385 17 1452 86
Cuts at: 6424 6436 6782 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 857 842 446 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4059 4059
Size: 6787
BtgI C'CryG_G
Cuts at: 513 1065 2004 3103 3317 3706 513
Size: 552 939 1099 214 389 3594
Fragments arranged by size:
3594 1099 939 552 389 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3006 487
Size: 683 56 1780 4268
Fragments arranged by size:
4268 1780 683 56
BtsI GCAGTG_nn'
Cuts at: 2235 2237 3139 3593 3820 3931 4303 5097 5117
Size: 2 902 454 227 111 372 794 20
Cuts at: 5117 2235
Size: 3905
Fragments arranged by size:
3905 902 794 454 372 227 111 20
2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6787
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DraIII CAC_nnn'GTG
Cuts at: 3208 3208
Size: 6787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5794 809
Size: 507 4478 1802
Fragments arranged by size:
4478 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2491 3068 4234
Size: 54 1425 50 275 18 661 577 1166
Cuts at: 4234 5123 6752 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1166 889 661 577 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4234 1537
Size: 275 2422 4090
Fragments arranged by size:
4090 2422 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 2983 3016 4717 1353
Size: 957 673 33 1701 3423
Fragments arranged by size:
3423 1701 957 673 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3424 3938 5374 5748 5894 387
Size: 1064 138 1835 514 1436 374 146 1280
Fragments arranged by size:
1835 1436 1280 1064 514 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2738 3215 4850 5447 6234 6502
Size: 393 770 336 477 1635 597 787 268
Cuts at: 6502 1239
Size: 1524
Fragments arranged by size:
1635 1524 787 770 597 477 393 336
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 2720
Size: 6787
EcoO109I rG'GnC_Cy
Cuts at: 2040 2195 2486 2593 2594 2610 2634 2884 3140
Size: 155 291 107 1 16 24 250 256
Cuts at: 3140 3141 3838 4035 2040
Size: 1 697 197 4792
Fragments arranged by size:
4792 697 291 256 250 197 155 107
24 16 1 1
EcoRI G'AATT_C
Cuts at: 4096 4474 4096
Size: 378 6409
FspI TGC'GCA
Cuts at: 5293 5293
Size: 6787
HaeII r_GCGC'y
Cuts at: 1678 2526 3807 5934 1678
Size: 848 1281 2127 2531
Fragments arranged by size:
2531 2127 1281 848
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2960 2981 2987 2992 2993
Size: 32 135 32 1388 21 6 5 1
Cuts at: 2993 3013 3019 3025 3694 3726 3968 4000 5428
Size: 20 6 6 669 32 242 32 1428
Cuts at: 5428 5460 5502 5534 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1428 1388 669 242 135 42 32
32 32 32 32 32 21 20 6
6 6 5 1
HincII GTy'rAC
Cuts at: 669 1657 1938 4131 4377 669
Size: 988 281 2193 246 3079
Fragments arranged by size:
3079 2193 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4008 748
Size: 3260 3527
HpaI GTT'AAC
Cuts at: 4377 4377
Size: 6787
KasI G'GCGC_C
Cuts at: 2522 2522
Size: 6787
KpnI G_GTAC'C
Cuts at: 4112 4112
Size: 6787
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 3030 3288 5901 6085 1305
Size: 1719 6 258 2613 184 2007
Fragments arranged by size:
2613 2007 1719 258 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4965
Fragments arranged by size:
4965 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2261 4745 5104 5263 518
Size: 648 843 252 2484 359 159 2042
Fragments arranged by size:
2484 2042 843 648 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2127 2261 2891 2950 3153 3193 3319
Size: 512 9 134 630 59 203 40 126
Cuts at: 3319 3363 3477 3624 3799 3943 3954 4028 4053
Size: 44 114 147 175 144 11 74 25
Cuts at: 4053 4203 4880 6028 6273 1606
Size: 150 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 512 245 203 175
150 147 144 134 126 114 74 59
44 40 25 11 9
NaeI GCC'GGC
Cuts at: 3407 3407
Size: 6787
NarI GG'CG_CC
Cuts at: 2523 2523
Size: 6787
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5296
Fragments arranged by size:
5296 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6787
NgoMIV G'CCGG_C
Cuts at: 3405 3405
Size: 6787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 4234 4234
Size: 6787
NspI r_CATG'y
Cuts at: 1132 1229 3340 4145 5690 1132
Size: 97 2111 805 1545 2229
Fragments arranged by size:
2229 2111 1545 805 97
PasI CC'CwG_GG
Cuts at: 1348 2787 2828 3160 3580 1348
Size: 1439 41 332 420 4555
Fragments arranged by size:
4555 1439 420 332 41
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2430 2953 1108
Size: 1322 523 4942
Fragments arranged by size:
4942 1322 523
PfoI T'CCnGG_A
Cuts at: 3612 3654 3612
Size: 42 6745
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PmlI CAC'GTG
Cuts at: 3205 3205
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 6369 6401 4856
Size: 32 1481 32 5242
Fragments arranged by size:
5242 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3838 3838
Size: 6787
PshAI GACnn'nnGTC
Cuts at: 1613 3378 3485 1613
Size: 1765 107 4915
Fragments arranged by size:
4915 1765 107
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PspOMI G'GGCC_C
Cuts at: 2039 2195 2593 2609 2633 2884 3140 3163 4034
Size: 156 398 16 24 251 256 23 871
Cuts at: 4034 2039
Size: 4792
Fragments arranged by size:
4792 871 398 256 251 156 24 23
16
PstI C_TGCA'G
Cuts at: 830 2881 3537 3720 4139 830
Size: 2051 656 183 419 3478
Fragments arranged by size:
3478 2051 656 419 183
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6787
PvuII CAG'CTG
Cuts at: 2261 2950 3153 3193 3477 3799 4028 2261
Size: 689 203 40 284 322 229 5020
Fragments arranged by size:
5020 689 322 284 229 203 40
SacII CC_GC'GG
Cuts at: 3320 3320
Size: 6787
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SanDI GG'GwC_CC
Cuts at: 3838 3838
Size: 6787
SbfI CC_TGCA'GG
Cuts at: 3537 4139 3537
Size: 602 6185
ScaI AGT'ACT
Cuts at: 5035 6747 5035
Size: 1712 5075
SfcI C'TryA_G
Cuts at: 826 1045 2877 3533 3716 4135 5270 5951 6142
Size: 219 1832 656 183 419 1135 681 191
Cuts at: 6142 826
Size: 1471
Fragments arranged by size:
1832 1471 1135 681 656 419 219 191
183
SfoI GGC'GCC
Cuts at: 2524 2524
Size: 6787
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6787
SmaI CCC'GGG
Cuts at: 1872 2200 2772 3077 3281 4114 1872
Size: 328 572 305 204 833 4545
Fragments arranged by size:
4545 833 572 328 305 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4076 4891 5792 6054 820
Size: 197 926 2133 815 901 262 1553
Fragments arranged by size:
2133 1553 926 901 815 262 197
SnaBI TAC'GTA
Cuts at: 493 4578 493
Size: 4085 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6787
SphI G_CATG'C
Cuts at: 1132 3340 4145 1132
Size: 2208 805 3774
Fragments arranged by size:
3774 2208 805
SrfI GCCC'GGGC
Cuts at: 2200 2772 2200
Size: 572 6215
SspI AAT'ATT
Cuts at: 5 52 4711 5
Size: 47 4659 2081
Fragments arranged by size:
4659 2081 47
StuI AGG'CCT
Cuts at: 1517 3531 1517
Size: 2014 4773
StyI C'CwwG_G
Cuts at: 513 1065 2004 2681 3249 4226 513
Size: 552 939 677 568 977 3074
Fragments arranged by size:
3074 977 939 677 568 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 2717 4814 4831 4984 5169 5614
Size: 1255 885 252 2097 17 153 185 445
Cuts at: 5614 325
Size: 1498
Fragments arranged by size:
2097 1498 1255 885 445 252 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2345 5033 6745
Size: 274 80 33 51 1443 368 2688 1712
Cuts at: 6745 96
Size: 138
Fragments arranged by size:
2688 1712 1443 368 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6747 6779 6747
Size: 32 6755
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6787
XbaI T'CTAG_A
Cuts at: 4123 4123
Size: 6787
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6787
XmaI C'CCGG_G
Cuts at: 1870 2198 2770 3075 3279 4112 1870
Size: 328 572 305 204 833 4545
Fragments arranged by size:
4545 833 572 328 305 204
XmnI GAAnn'nnTTC
Cuts at: 2186 4916 2186
Size: 2730 4057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 276
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SacI SapI
SexAI SfiI SwaI XcmI