Restriction map for
pFN21AE2401
(Circular) (Six-base) MAPSORT of: pFN21AE2401.seq Check: 4734 from: 1 to: 5806
pFN21AE2401 5806 bp
With 182 enzymes: SgfI *
March 8, 2012 13:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 3105 3869 5253 5521 1632
Size: 1059 414 764 1384 268 1917
Fragments arranged by size:
1917 1384 1059 764 414 268
AfeI AGC'GCT
Cuts at: 2126 3082 2126
Size: 956 4850
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3058 3090 3058
Size: 32 5774
AlwNI CAG_nnn'CTG
Cuts at: 2539 3549 5121 2539
Size: 1010 1572 3224
Fragments arranged by size:
3224 1572 1010
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3014 3115 3341 3493 5602 1493
Size: 1521 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1521 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2050 2078 2693 3131 1870
Size: 73 87 20 28 615 438 4545
Fragments arranged by size:
4545 615 438 87 73 28 20
BamHI G'GATC_C
Cuts at: 3136 3503 3136
Size: 367 5439
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3127 4582 618
Size: 325 217 627 381 959 1455 1842
Fragments arranged by size:
1842 1455 959 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2031 2042 2089 3125 1391
Size: 640 11 47 1036 4072
Fragments arranged by size:
4072 1036 640 47 11
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 5806
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BbvCI CC'TCA_GC
Cuts at: 2794 2794
Size: 5806
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BfrBI ATG'CAT
Cuts at: 2643 2643
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2240 2834 4417
Size: 107 122 71 631 605 568 594 1583
Cuts at: 4417 136
Size: 1525
Fragments arranged by size:
1583 1525 631 605 594 568 122 107
71
BglII A'GATC_T
Cuts at: 3006 5801 3006
Size: 2795 3011
BlpI GC'TnA_GC
Cuts at: 3223 3223
Size: 5806
Bme1580I G_kGCm'C
Cuts at: 1790 2943 3867 5023 1790
Size: 1153 924 1156 2573
Fragments arranged by size:
2573 1156 1153 924
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2733 4466 1239
Size: 1494 1733 2579
Fragments arranged by size:
2579 1733 1494
Bpu10I CC'TnA_GC
Cuts at: 2794 2794
Size: 5806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3931 4796 5094 3931
Size: 865 298 4643
Fragments arranged by size:
4643 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2967 3613 3995
Size: 53 83 186 936 636 798 646 382
Cuts at: 3995 4630 275
Size: 635 1451
Fragments arranged by size:
1451 936 798 646 636 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5581
Fragments arranged by size:
5581 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2407 2422 2425 2434 2437 2440 2851 2854
Size: 1221 15 3 9 3 3 411 3
Cuts at: 2854 2857 2866 1186
Size: 3 9 4126
Fragments arranged by size:
4126 1221 411 15 9 9 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2219 2539 2902 3077 5009 5505 2219
Size: 320 363 175 1932 496 2520
Fragments arranged by size:
2520 1932 496 363 320 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5233
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2244 3256 4017 5045 1540
Size: 275 186 243 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 243 186
BsiHKAI G_wGCw'C
Cuts at: 3867 3952 5023 3867
Size: 85 1071 4650
Fragments arranged by size:
4650 1071 85
BsmI GAATG_Cn'
Cuts at: 2645 3089 3317 3410 2645
Size: 444 228 93 5041
Fragments arranged by size:
5041 444 228 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2042 2089 2943 3125 3867 3952
Size: 399 241 11 47 854 182 742 85
Cuts at: 3952 5023 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 854 742 399 241 182 85
47 11
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 2763 3695 5425 2763
Size: 932 1730 3144
Fragments arranged by size:
3144 1730 932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3161 844
Size: 2317 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2133 3693 1394
Size: 429 310 1560 3507
Fragments arranged by size:
3507 1560 429 310
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2468 4450 4637 1700
Size: 110 658 1982 187 2869
Fragments arranged by size:
2869 1982 658 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 2195 2249 1925
Size: 270 54 5482
Fragments arranged by size:
5482 270 54
BssSI C'ACGA_G
Cuts at: 3866 4878 3866
Size: 1012 4794
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2979 794
Size: 2185 3621
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 3006 3136 3503 3888 3905 5357 5443 5455
Size: 1034 130 367 385 17 1452 86 12
Cuts at: 5455 5801 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1034 385 367 346 130 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2878 2878
Size: 5806
BtgI C'CryG_G
Cuts at: 513 1065 2238 513
Size: 552 1173 4081
Fragments arranged by size:
4081 1173 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 2617 3322 4116 4136 2617
Size: 705 794 20 4287
Fragments arranged by size:
4287 794 705 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4813 809
Size: 507 3497 1802
Fragments arranged by size:
3497 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2241 2286
Size: 54 1425 50 275 18 354 57 45
Cuts at: 2286 3253 4142 5771 8
Size: 967 889 1629 43
Fragments arranged by size:
1629 1425 967 889 354 275 57 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2241 3253 1537
Size: 275 429 1012 4090
Fragments arranged by size:
4090 1012 429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2909 3024 3736 1353
Size: 1556 115 712 3423
Fragments arranged by size:
3423 1556 712 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2391 2410 2428 2907 4393 4767
Size: 1064 138 802 19 18 479 1486 374
Cuts at: 4767 4913 387
Size: 146 1280
Fragments arranged by size:
1486 1280 1064 802 479 374 146 138
19 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2691 2733 3105 3869 4466 5253 5521
Size: 393 1059 42 372 764 597 787 268
Cuts at: 5521 1239
Size: 1524
Fragments arranged by size:
1524 1059 787 764 597 393 372 268
42
EcoNI CCTnn'n_nnAGG
Cuts at: 2947 2947
Size: 5806
EcoO109I rG'GnC_Cy
Cuts at: 2047 2047
Size: 5806
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2128 2172 2229 2667 3084 4953 1678
Size: 450 44 57 438 417 1869 2531
Fragments arranged by size:
2531 1869 450 438 417 57 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2671 2703 2991
Size: 32 135 32 524 32 543 32 288
Cuts at: 2991 3023 4447 4479 4521 4553 1373
Size: 32 1424 32 42 32 2626
Fragments arranged by size:
2626 1424 543 524 288 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 5806
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2309 2762 3094 3764 4123 4282
Size: 648 1092 51 453 332 670 359 159
Cuts at: 4282 518
Size: 2042
Fragments arranged by size:
2042 1092 670 648 453 359 332 159
51
MspA1I CmG'CkG
Cuts at: 1606 2240 2325 3222 3899 5047 5292 1606
Size: 634 85 897 677 1148 245 2120
Fragments arranged by size:
2120 1148 897 677 634 245 85
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 5806
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 5806
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5254
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 2241 3253 2241
Size: 1012 4794
NsiI A_TGCA'T
Cuts at: 2645 2645
Size: 5806
NspI r_CATG'y
Cuts at: 1132 1229 2263 2515 3164 4709 1132
Size: 97 1034 252 649 1545 2229
Fragments arranged by size:
2229 1545 1034 649 252 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2316 2539 1108
Size: 1208 223 4375
Fragments arranged by size:
4375 1208 223
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PstI C_TGCA'G
Cuts at: 830 2624 2975 3158 830
Size: 1794 351 183 3478
Fragments arranged by size:
3478 1794 351 183
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
SacII CC_GC'GG
Cuts at: 2241 2241
Size: 5806
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SapI GCTCTTCn'nnn_
Cuts at: 3024 3024
Size: 5806
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SfcI C'TryA_G
Cuts at: 826 1045 2620 2971 3154 4289 4970 5161 826
Size: 219 1575 351 183 1135 681 191 1471
Fragments arranged by size:
1575 1471 1135 681 351 219 191 183
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 5806
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 2468 1700
Size: 768 5038
SmaI CCC'GGG
Cuts at: 1872 2032 2052 3133 1872
Size: 160 20 1081 4545
Fragments arranged by size:
4545 1081 160 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 4811 5073 820
Size: 197 926 1967 901 262 1553
Fragments arranged by size:
1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 2515 3164 1132
Size: 1383 649 3774
Fragments arranged by size:
3774 1383 649
SrfI GCCC'GGGC
Cuts at: 2032 2032
Size: 5806
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5806
StyI C'CwwG_G
Cuts at: 513 1065 2061 3245 513
Size: 552 996 1184 3074
Fragments arranged by size:
3074 1184 996 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 3850 4003 4188 4633 325
Size: 1255 2253 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2911 2943 5766 5798 2911
Size: 32 2823 32 2919
Fragments arranged by size:
2919 2823 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2020
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2030 2050 3131 1870
Size: 160 20 1081 4545
Fragments arranged by size:
4545 1081 160 20
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SwaI