Restriction map for
pFN21AE2397
(Circular) (Six-base) MAPSORT of: pFN21AE2397.seq Check: 8695 from: 1 to: 7612
pFN21AE2397 7612 bp
With 182 enzymes: SgfI *
February 7, 2012 16:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2337 2337
Size: 7612
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4038 5422 278
Size: 53 83 186 936 2502 1384 2468
Fragments arranged by size:
2502 2468 1384 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2428 4955 1937
Size: 491 2527 4594
Fragments arranged by size:
4594 2527 491
Acc65I G'GTAC_C
Cuts at: 4933 4933
Size: 7612
AclI AA'CG_TT
Cuts at: 1563 5739 6112 1563
Size: 4176 373 3063
Fragments arranged by size:
4176 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3357 4464 5675 7059 7327 1632
Size: 1725 1107 1211 1384 268 1917
Fragments arranged by size:
1917 1725 1384 1211 1107 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7415
AflIII A'CryG_T
Cuts at: 1203 6511 7488 1203
Size: 5308 977 1327
Fragments arranged by size:
5308 1327 977
AgeI A'CCGG_T
Cuts at: 6443 6443
Size: 7612
AhdI GACnn_n'nnGTC
Cuts at: 6341 6341
Size: 7612
AleI CACnn'nnGTG
Cuts at: 1166 3617 1166
Size: 2451 5161
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3564 3888 4013 4088 1184
Size: 2380 324 125 75 4708
Fragments arranged by size:
4708 2380 324 125 75
AlwNI CAG_nnn'CTG
Cuts at: 2990 4779 5355 6927 2990
Size: 1789 576 1572 3675
Fragments arranged by size:
3675 1789 1572 576
ApaLI G'TGCA_C
Cuts at: 2519 5669 6825 2519
Size: 3150 1156 3306
Fragments arranged by size:
3306 3150 1156
ApoI r'AATT_y
Cuts at: 1493 2808 4479 4921 5147 5299 7408 1493
Size: 1315 1671 442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1671 1315 442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2970 6166 160
Size: 872 1938 3196 1606
Fragments arranged by size:
3196 1938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7612
AvaI C'yCGr_G
Cuts at: 1870 1943 4937 1870
Size: 73 2994 4545
Fragments arranged by size:
4545 2994 73
BamHI G'GATC_C
Cuts at: 2056 4942 5309 2056
Size: 2886 367 4359
Fragments arranged by size:
4359 2886 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 2694 3143 4841 4933
Size: 325 217 627 432 475 449 1698 92
Cuts at: 4933 6388 618
Size: 1455 1842
Fragments arranged by size:
1842 1698 1455 627 475 449 432 325
217 92
BanII G_rGCy'C
Cuts at: 1391 3420 4761 4931 1391
Size: 2029 1341 170 4072
Fragments arranged by size:
4072 2029 1341 170
BbeI G_GCGC'C
Cuts at: 2698 2698
Size: 7612
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2935 4700 928
Size: 971 1036 1765 3840
Fragments arranged by size:
3840 1765 1036 971
BbvCI CC'TCA_GC
Cuts at: 2633 3812 2633
Size: 1179 6433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5803 5837 1903
Size: 34 3866 34 3678
Fragments arranged by size:
3866 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4527 5504 6714 4527
Size: 977 1210 5425
Fragments arranged by size:
5425 1210 977
BclI T'GATC_A
Cuts at: 1548 3777 1548
Size: 2229 5383
BfrBI ATG'CAT
Cuts at: 2747 2747
Size: 7612
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4028 6223 136
Size: 107 122 71 631 605 2356 2195 1525
Fragments arranged by size:
2356 2195 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7607 7607
Size: 7612
BlpI GC'TnA_GC
Cuts at: 5029 5029
Size: 7612
Bme1580I G_kGCm'C
Cuts at: 1790 2523 3197 5673 6829 1790
Size: 733 674 2476 1156 2573
Fragments arranged by size:
2573 2476 1156 733 674
BmgBI CAC'GTC
Cuts at: 1318 3580 1318
Size: 2262 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4510 4682 5343
Size: 416 53 83 399 24 3093 172 661
Cuts at: 5343 6301 442
Size: 958 1753
Fragments arranged by size:
3093 1753 958 661 416 399 172 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7612
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4581 4581
Size: 7612
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2817 3174 3510 4145 4666 6272 1239
Size: 1578 357 336 635 521 1606 2579
Fragments arranged by size:
2579 1606 1578 635 521 357 336
Bpu10I CC'TnA_GC
Cuts at: 2633 3812 3830 4044 2633
Size: 1179 18 214 6201
Fragments arranged by size:
6201 1179 214 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3896 5737 6602 6900 3896
Size: 1841 865 298 4608
Fragments arranged by size:
4608 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4603 6275 882
Size: 617 3104 1672 2219
Fragments arranged by size:
3104 2219 1672 617
BsaAI yAC'GTr
Cuts at: 493 1204 5403 493
Size: 711 4199 2702
Fragments arranged by size:
4199 2702 711
BsaBI GATnn'nnATC
Cuts at: 7606 7606
Size: 7612
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2695 2718 3758 4035
Size: 53 83 186 936 1162 23 1040 277
Cuts at: 4035 5419 5801 6436 275
Size: 1384 382 635 1451
Fragments arranged by size:
1451 1384 1162 1040 936 635 382 277
186 83 53 23
BsaWI w'CCGG_w
Cuts at: 2668 6045 6443 6717 6864 7275 2668
Size: 3377 398 274 147 411 3005
Fragments arranged by size:
3377 3005 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4040 4070 1178
Size: 30 165 30 2637 30 4720
Fragments arranged by size:
4720 2637 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3201 1186
Size: 2015 5597
BseYI C'CCAG_C
Cuts at: 2569 3290 3419 3818 4025 4187 6815 7311 2569
Size: 721 129 399 207 162 2628 496 2870
Fragments arranged by size:
2870 2628 721 496 399 207 162 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 3104 3641 4058 1163
Size: 573 623 745 537 417 4717
Fragments arranged by size:
4717 745 623 573 537 417
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5062 5823 6851 1540
Size: 275 186 3061 761 1028 2301
Fragments arranged by size:
3061 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 4092 4761 5673 5758 6829 2523
Size: 1569 669 912 85 1071 3306
Fragments arranged by size:
3306 1569 1071 912 669 85
BsmI GAATG_Cn'
Cuts at: 3970 5123 5216 3970
Size: 1153 93 6366
Fragments arranged by size:
6366 1153 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3570 3570
Size: 7612
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2523 3197 3420 4092 4761 4931 5673
Size: 399 733 674 223 672 669 170 742
Cuts at: 5673 5758 6829 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 733 674 672 669 399
223 170 85
BspEI T'CCGG_A
Cuts at: 2668 7275 2668
Size: 4607 3005
BspHI T'CATG_A
Cuts at: 5501 7231 5501
Size: 1730 5882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2337 4345 4967 844
Size: 1493 2008 622 3489
Fragments arranged by size:
3489 2008 1493 622
BsrBI CCG'CTC
Cuts at: 1394 1823 5499 1394
Size: 429 3676 3507
Fragments arranged by size:
3676 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6107 6281 65
Size: 1183 4859 174 1396
Fragments arranged by size:
4859 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2562 6256 6443 1700
Size: 110 752 3694 187 2869
Fragments arranged by size:
3694 2869 752 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7612
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7612
BssSI C'ACGA_G
Cuts at: 5672 6684 5672
Size: 1012 6600
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7612
BstBI TT'CG_AA
Cuts at: 6416 6416
Size: 7612
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7612
BstXI CCAn_nnnn'nTGG
Cuts at: 2401 2507 2626 4207 4880 2401
Size: 106 119 1581 673 5133
Fragments arranged by size:
5133 1581 673 119 106
BstYI r'GATC_y
Cuts at: 1972 2056 2370 3688 4237 4942 5309 5694 5711
Size: 84 314 1318 549 705 367 385 17
Cuts at: 5711 7163 7249 7261 7607 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1318 705 549 385 367 346
314 86 84 17 12
BstZ17I GTA'TAC
Cuts at: 2429 2429
Size: 7612
Bsu36I CC'TnA_GG
Cuts at: 2253 2253
Size: 7612
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 2706 3309 513
Size: 552 939 199 503 603 4816
Fragments arranged by size:
4816 939 603 552 503 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6873
Fragments arranged by size:
6873 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3612 3780 4070 4847 5128 5922 5942 2111
Size: 1501 168 290 777 281 794 20 3781
Fragments arranged by size:
3781 1501 794 777 290 281 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7577
DraI TTT'AAA
Cuts at: 4916 4916
Size: 7612
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2405 6619 809
Size: 507 1089 4214 1802
Fragments arranged by size:
4214 1802 1089 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3594 4020 4341
Size: 54 1425 50 275 18 1764 426 321
Cuts at: 4341 5059 5948 7577 8
Size: 718 889 1629 43
Fragments arranged by size:
1764 1629 1425 889 718 426 321 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5059 1537
Size: 275 3247 4090
Fragments arranged by size:
4090 3247 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2859 3474 4221 5542 1353
Size: 1506 615 747 1321 3423
Fragments arranged by size:
3423 1506 1321 747 615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 3174 6199 6573 6719 387
Size: 1064 138 996 589 3025 374 146 1280
Fragments arranged by size:
3025 1280 1064 996 589 374 146 138
EcoICRI GAG'CTC
Cuts at: 4759 4759
Size: 7612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2817 3174 3357 3510 4145 4464 4666
Size: 393 1185 357 183 153 635 319 202
Cuts at: 4666 5675 6272 7059 7327 1239
Size: 1009 597 787 268 1524
Fragments arranged by size:
1524 1185 1009 787 635 597 393 357
319 268 202 183 153
EcoNI CCTnn'n_nnAGG
Cuts at: 4738 4899 4738
Size: 161 7451
EcoO109I rG'GnC_Cy
Cuts at: 3331 4630 4892 3331
Size: 1299 262 6051
Fragments arranged by size:
6051 1299 262
EcoRI G'AATT_C
Cuts at: 4921 5299 4921
Size: 378 7234
FspI TGC'GCA
Cuts at: 6118 6118
Size: 7612
HaeII r_GCGC'y
Cuts at: 1678 2698 3607 6759 1678
Size: 1020 909 3152 2531
Fragments arranged by size:
3152 2531 1020 909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3079 3111 3305 3337 3730
Size: 32 135 32 1507 32 194 32 393
Cuts at: 3730 3762 4040 4072 4085 4117 4385 4417 4687
Size: 32 278 32 13 32 268 32 270
Cuts at: 4687 4719 6253 6285 6327 6359 1373
Size: 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 1507 393 278 270 268 194
135 42 32 32 32 32 32 32
32 32 32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 4956 5202 669
Size: 988 281 3018 246 3079
Fragments arranged by size:
3079 3018 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7612
HpaI GTT'AAC
Cuts at: 5202 5202
Size: 7612
KasI G'GCGC_C
Cuts at: 2694 2694
Size: 7612
KpnI G_GTAC'C
Cuts at: 4937 4937
Size: 7612
MfeI C'AATT_G
Cuts at: 4659 5211 4659
Size: 552 7060
MluI A'CGCG_T
Cuts at: 7488 7488
Size: 7612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2775 6726 6910 1305
Size: 1470 3951 184 2007
Fragments arranged by size:
3951 2007 1470 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4343 10
Size: 54 1425 343 2511 3279
Fragments arranged by size:
3279 2511 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 3546 3617 4878 5570 5929 6088
Size: 648 1165 1215 71 1261 692 359 159
Cuts at: 6088 518
Size: 2042
Fragments arranged by size:
2042 1261 1215 1165 692 648 359 159
71
MspA1I CmG'CkG
Cuts at: 1606 2569 2783 4025 4187 4350 4438 4462 4788
Size: 963 214 1242 162 163 88 24 326
Cuts at: 4788 5028 5705 6853 7098 1606
Size: 240 677 1148 245 2120
Fragments arranged by size:
2120 1242 1148 963 677 326 245 240
214 163 162 88 24
NarI GG'CG_CC
Cuts at: 2695 2695
Size: 7612
NcoI C'CATG_G
Cuts at: 513 1065 2004 2203 3309 513
Size: 552 939 199 1106 4816
Fragments arranged by size:
4816 1106 939 552 199
NdeI CA'TA_TG
Cuts at: 387 4384 387
Size: 3997 3615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7612
NotI GC'GGCC_GC
Cuts at: 5059 5059
Size: 7612
NsiI A_TGCA'T
Cuts at: 2749 2749
Size: 7612
NspI r_CATG'y
Cuts at: 1132 1229 2336 3169 3545 4970 6515 1132
Size: 97 1107 833 376 1425 1545 2229
Fragments arranged by size:
2229 1545 1425 1107 833 376 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7612
PciI A'CATG_T
Cuts at: 6511 6511
Size: 7612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2204 2344 3233 4350 1108
Size: 1096 140 889 1117 4370
Fragments arranged by size:
4370 1117 1096 889 140
PfoI T'CCnGG_A
Cuts at: 2506 3268 4122 2506
Size: 762 854 5996
Fragments arranged by size:
5996 854 762
PmeI GTTT'AAAC
Cuts at: 4916 4916
Size: 7612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4315 4347 5681 5713 7194 7226 4315
Size: 32 1334 32 1481 32 4701
Fragments arranged by size:
4701 1481 1334 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3331 4892 3331
Size: 1561 6051
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7612
PsiI TTA'TAA
Cuts at: 5182 5182
Size: 7612
PstI C_TGCA'G
Cuts at: 830 2613 4461 4964 830
Size: 1783 1848 503 3478
Fragments arranged by size:
3478 1848 1783 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7612
PvuII CAG'CTG
Cuts at: 2569 2783 4187 4350 4438 4462 2569
Size: 214 1404 163 88 24 5719
Fragments arranged by size:
5719 1404 214 163 88 24
SacI G_AGCT'C
Cuts at: 4761 4761
Size: 7612
SalI G'TCGA_C
Cuts at: 1936 4954 1936
Size: 3018 4594
SapI GCTCTTCn'nnn_
Cuts at: 4221 4221
Size: 7612
SbfI CC_TGCA'GG
Cuts at: 4964 4964
Size: 7612
ScaI AGT'ACT
Cuts at: 4150 5860 7572 4150
Size: 1710 1712 4190
Fragments arranged by size:
4190 1712 1710
SexAI A'CCwGG_T
Cuts at: 2215 2215
Size: 7612
SfcI C'TryA_G
Cuts at: 826 1045 2609 4457 4960 6095 6776 6967 826
Size: 219 1564 1848 503 1135 681 191 1471
Fragments arranged by size:
1848 1564 1471 1135 681 503 219 191
SfoI GGC'GCC
Cuts at: 2696 2696
Size: 7612
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7612
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7612
SmaI CCC'GGG
Cuts at: 1872 4939 1872
Size: 3067 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3911 5716 6617 6879 820
Size: 197 926 1968 1805 901 262 1553
Fragments arranged by size:
1968 1805 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5403 493
Size: 4910 2702
SpeI A'CTAG_T
Cuts at: 152 3029 152
Size: 2877 4735
SphI G_CATG'C
Cuts at: 1132 2336 3545 4970 1132
Size: 1204 1209 1425 3774
Fragments arranged by size:
3774 1425 1209 1204
SspI AAT'ATT
Cuts at: 5 52 2079 2145 2974 5536 5
Size: 47 2027 66 829 2562 2081
Fragments arranged by size:
2562 2081 2027 829 66 47
StuI AGG'CCT
Cuts at: 1517 2035 1517
Size: 518 7094
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 2394 2619 3309 5051 513
Size: 552 939 199 191 225 690 1742 3074
Fragments arranged by size:
3074 1742 939 690 552 225 199 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5639 5656 5809 5994 6439 325
Size: 1255 4059 17 153 185 445 1498
Fragments arranged by size:
4059 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3203 4148 5858
Size: 274 80 33 51 1443 1226 945 1710
Cuts at: 5858 7570 96
Size: 1712 138
Fragments arranged by size:
1712 1710 1443 1226 945 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 7572 7604 2502
Size: 32 5038 32 2510
Fragments arranged by size:
5038 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3641 4775 1316
Size: 2325 1134 4153
Fragments arranged by size:
4153 2325 1134
XbaI T'CTAG_A
Cuts at: 3511 4333 4897 4948 3511
Size: 822 564 51 6175
Fragments arranged by size:
6175 822 564 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 4497 4579 2401
Size: 2096 82 5434
Fragments arranged by size:
5434 2096 82
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7612
XmaI C'CCGG_G
Cuts at: 1870 4937 1870
Size: 3067 4545
XmnI GAAnn'nnTTC
Cuts at: 2094 5741 2094
Size: 3647 3965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4036 5420 276
Size: 53 83 186 936 2502 1384 2468
Fragments arranged by size:
2502 2468 1384 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI DraIII
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI