Restriction map for
pFN21AE2394
(Circular) (Six-base) MAPSORT of: pFN21AE2394.seq Check: 7544 from: 1 to: 7168
pFN21AE2394 7168 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2413 2413
Size: 7168
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2766 3497 4978 278
Size: 53 83 186 936 1230 731 1481 2468
Fragments arranged by size:
2468 1481 1230 936 731 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3255 4511 1937
Size: 1318 1256 4594
Fragments arranged by size:
4594 1318 1256
Acc65I G'GTAC_C
Cuts at: 4489 4489
Size: 7168
AclI AA'CG_TT
Cuts at: 1563 5295 5668 1563
Size: 3732 373 3063
Fragments arranged by size:
3732 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2715 3909 4311 5231 6615 6883 1632
Size: 1083 1194 402 920 1384 268 1917
Fragments arranged by size:
1917 1384 1194 1083 920 402 268
AflII C'TTAA_G
Cuts at: 820 1017 4141 820
Size: 197 3124 3847
Fragments arranged by size:
3847 3124 197
AflIII A'CryG_T
Cuts at: 1203 3934 6067 7044 1203
Size: 2731 2133 977 1327
Fragments arranged by size:
2731 2133 1327 977
AgeI A'CCGG_T
Cuts at: 5999 5999
Size: 7168
AhdI GACnn_n'nnGTC
Cuts at: 5897 5897
Size: 7168
AleI CACnn'nnGTG
Cuts at: 1166 3912 1166
Size: 2746 4422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7168
AlwNI CAG_nnn'CTG
Cuts at: 2050 2089 3299 4911 6483 2050
Size: 39 1210 1612 1572 2735
Fragments arranged by size:
2735 1612 1572 1210 39
ApaI G_GGCC'C
Cuts at: 2863 2863
Size: 7168
ApaLI G'TGCA_C
Cuts at: 5225 6381 5225
Size: 1156 6012
ApoI r'AATT_y
Cuts at: 1493 3386 3565 4477 4703 4855 6964 1493
Size: 1893 179 912 226 152 2109 1697
Fragments arranged by size:
2109 1893 1697 912 226 179 152
AseI AT'TA_AT
Cuts at: 160 1032 5722 160
Size: 872 4690 1606
Fragments arranged by size:
4690 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
AvaI C'yCGr_G
Cuts at: 1870 1943 2652 4493 1870
Size: 73 709 1841 4545
Fragments arranged by size:
4545 1841 709 73
BamHI G'GATC_C
Cuts at: 4498 4865 4498
Size: 367 6801
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 4489 5944 618
Size: 325 217 627 246 2456 1455 1842
Fragments arranged by size:
2456 1842 1455 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2382 2863 2949 4374 4487 1391
Size: 991 481 86 1425 113 4072
Fragments arranged by size:
4072 1425 991 481 113 86
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 7168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3831 4113 928
Size: 971 1932 282 3983
Fragments arranged by size:
3983 1932 971 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5359 5393 1903
Size: 34 3422 34 3678
Fragments arranged by size:
3678 3422 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2578 2672 5060 6270 2578
Size: 94 2388 1210 3476
Fragments arranged by size:
3476 2388 1210 94
BclI T'GATC_A
Cuts at: 1548 2496 4354 1548
Size: 948 1858 4362
Fragments arranged by size:
4362 1858 948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5779 136
Size: 107 122 71 631 605 4107 1525
Fragments arranged by size:
4107 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7163 7163
Size: 7168
BlpI GC'TnA_GC
Cuts at: 2643 2774 4585 2643
Size: 131 1811 5226
Fragments arranged by size:
5226 1811 131
Bme1580I G_kGCm'C
Cuts at: 1790 2863 5229 6385 1790
Size: 1073 2366 1156 2573
Fragments arranged by size:
2573 2366 1156 1073
BmgBI CAC'GTC
Cuts at: 1318 3403 1318
Size: 2085 5083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2435 4899 5857
Size: 416 53 83 399 24 1018 2464 958
Cuts at: 5857 442
Size: 1753
Fragments arranged by size:
2464 1753 1018 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7168
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 4303 2401
Size: 1902 5266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 3363 3846 5828 1239
Size: 833 1291 483 1982 2579
Fragments arranged by size:
2579 1982 1291 833 483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2697 3206 3887 5293 6158 6456 2697
Size: 509 681 1406 865 298 3409
Fragments arranged by size:
3409 1406 865 681 509 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2627 5831 882
Size: 617 1128 3204 2219
Fragments arranged by size:
3204 2219 1128 617
BsaAI yAC'GTr
Cuts at: 493 1204 2792 3229 4959 493
Size: 711 1588 437 1730 2702
Fragments arranged by size:
2702 1730 1588 711 437
BsaBI GATnn'nnATC
Cuts at: 3036 3544 3996 7162 3036
Size: 508 452 3166 3042
Fragments arranged by size:
3166 3042 508 452
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 2763 3494 4975
Size: 53 83 186 936 501 729 731 1481
Cuts at: 4975 5357 5992 275
Size: 382 635 1451
Fragments arranged by size:
1481 1451 936 731 729 635 501 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3951 5601 5999 6273 6420 6831 3951
Size: 1650 398 274 147 411 4288
Fragments arranged by size:
4288 1650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2999 3029 1178
Size: 30 165 30 1596 30 5317
Fragments arranged by size:
5317 1596 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2261 2584 3248 4252 1186
Size: 937 138 323 664 1004 4102
Fragments arranged by size:
4102 1004 937 664 323 138
BseYI C'CCAG_C
Cuts at: 4147 6371 6867 4147
Size: 2224 496 4448
Fragments arranged by size:
4448 2224 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2814 1163
Size: 573 1078 5517
Fragments arranged by size:
5517 1078 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4618 5379 6407 1540
Size: 275 186 2617 761 1028 2301
Fragments arranged by size:
2617 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 2791 2949 4374 5229 5314 6385 2382
Size: 409 158 1425 855 85 1071 3165
Fragments arranged by size:
3165 1425 1071 855 409 158 85
BsmI GAATG_Cn'
Cuts at: 2342 2844 3670 4679 4772 2342
Size: 502 826 1009 93 4738
Fragments arranged by size:
4738 1009 826 502 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2770 3887 2770
Size: 1117 6051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2382 2791 2863 2949 4374 4487 5229
Size: 399 592 409 72 86 1425 113 742
Cuts at: 5229 5314 6385 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1425 1071 742 592 409 399 113
86 85 72
BspEI T'CCGG_A
Cuts at: 6831 6831
Size: 7168
BspHI T'CATG_A
Cuts at: 3322 3424 5057 6787 3322
Size: 102 1633 1730 3703
Fragments arranged by size:
3703 1730 1633 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2361 2413 4523 844
Size: 1517 52 2110 3489
Fragments arranged by size:
3489 2110 1517 52
BsrBI CCG'CTC
Cuts at: 1394 1823 2463 5055 1394
Size: 429 640 2592 3507
Fragments arranged by size:
3507 2592 640 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3091 3645 5663 5837 65
Size: 1183 1843 554 2018 174 1396
Fragments arranged by size:
2018 1843 1396 1183 554 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 5812 5999 1700
Size: 110 226 3776 187 2869
Fragments arranged by size:
3776 2869 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7168
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7168
BssSI C'ACGA_G
Cuts at: 5228 6240 5228
Size: 1012 6156
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2428 794
Size: 1634 5534
BstBI TT'CG_AA
Cuts at: 5972 5972
Size: 7168
BstEII G'GTnAC_C
Cuts at: 1184 3191 1184
Size: 2007 5161
BstXI CCAn_nnnn'nTGG
Cuts at: 3818 3818
Size: 7168
BstYI r'GATC_y
Cuts at: 1972 2313 4017 4426 4498 4865 5250 5267 6719
Size: 341 1704 409 72 367 385 17 1452
Cuts at: 6719 6805 6817 7163 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1704 1452 409 385 367 346 341
86 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5677
Fragments arranged by size:
5677 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2139 487
Size: 683 56 913 5516
Fragments arranged by size:
5516 913 683 56
BtsI GCAGTG_nn'
Cuts at: 3907 4684 5478 5498 3907
Size: 777 794 20 5577
Fragments arranged by size:
5577 794 777 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3035 3070 529
Size: 35 2471 35 4627
Fragments arranged by size:
4627 2471 35 35
DraI TTT'AAA
Cuts at: 4472 4472
Size: 7168
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6175 809
Size: 507 4859 1802
Fragments arranged by size:
4859 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 4615 5504
Size: 54 1425 50 275 18 240 2545 889
Cuts at: 5504 7133 8
Size: 1629 43
Fragments arranged by size:
2545 1629 1425 889 275 240 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4615 1537
Size: 275 2803 4090
Fragments arranged by size:
4090 2803 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3165 3605 4008 4209 5098 1353
Size: 1812 440 403 201 889 3423
Fragments arranged by size:
3423 1812 889 440 403 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3797 5755 6129 6275 387
Size: 1064 138 2208 1958 374 146 1280
Fragments arranged by size:
2208 1958 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2380 2947 4372 2380
Size: 567 1425 5176
Fragments arranged by size:
5176 1425 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2715 3363 3846 3909 4311 5231
Size: 393 440 643 648 483 63 402 920
Cuts at: 5231 5828 6615 6883 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 920 787 648 643 597 483 440
402 393 268 63
EcoNI CCTnn'n_nnAGG
Cuts at: 4141 4199 4141
Size: 58 7110
EcoO109I rG'GnC_Cy
Cuts at: 2860 2875 3153 2860
Size: 15 278 6875
Fragments arranged by size:
6875 278 15
EcoRI G'AATT_C
Cuts at: 4477 4855 4477
Size: 378 6790
EcoRV GAT'ATC
Cuts at: 3718 3728 3718
Size: 10 7158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2663 2749 4316 2663
Size: 86 1567 5515
Fragments arranged by size:
5515 1567 86
FspI TGC'GCA
Cuts at: 5674 5674
Size: 7168
HaeII r_GCGC'y
Cuts at: 1678 2037 6315 1678
Size: 359 4278 2531
Fragments arranged by size:
4278 2531 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2646 2678 3302 3334 3582
Size: 32 135 32 1074 32 624 32 248
Cuts at: 3582 3614 3717 3749 4208 4240 5809 5841 5883
Size: 32 103 32 459 32 1569 32 42
Cuts at: 5883 5915 1373
Size: 32 2626
Fragments arranged by size:
2626 1569 1074 624 459 248 135 103
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4252 4444 4512 4758 669
Size: 988 281 2314 192 68 246 3079
Fragments arranged by size:
3079 2314 988 281 246 192 68
HindIII A'AGCT_T
Cuts at: 748 2771 3945 748
Size: 2023 1174 3971
Fragments arranged by size:
3971 2023 1174
HpaI GTT'AAC
Cuts at: 4252 4444 4758 4252
Size: 192 314 6662
Fragments arranged by size:
6662 314 192
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 7168
KpnI G_GTAC'C
Cuts at: 4493 4493
Size: 7168
MfeI C'AATT_G
Cuts at: 3690 4767 3690
Size: 1077 6091
MluI A'CGCG_T
Cuts at: 7044 7044
Size: 7168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3949 6282 6466 1305
Size: 2644 2333 184 2007
Fragments arranged by size:
2644 2333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5346
Fragments arranged by size:
5346 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3084 3912 3977 5126 5485 5644 518
Size: 648 1918 828 65 1149 359 159 2042
Fragments arranged by size:
2042 1918 1149 828 648 359 159 65
MspA1I CmG'CkG
Cuts at: 1606 2024 2969 3299 4584 5261 6409 6654 1606
Size: 418 945 330 1285 677 1148 245 2120
Fragments arranged by size:
2120 1285 1148 945 677 418 330 245
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 7168
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 7168
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5677
Fragments arranged by size:
5677 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7168
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 7168
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7168
NotI GC'GGCC_GC
Cuts at: 4615 4615
Size: 7168
NspI r_CATG'y
Cuts at: 1132 1229 3938 4526 6071 1132
Size: 97 2709 588 1545 2229
Fragments arranged by size:
2709 2229 1545 588 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7168
PciI A'CATG_T
Cuts at: 3934 6067 3934
Size: 2133 5035
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2050 2933 3524 1108
Size: 942 883 591 4752
Fragments arranged by size:
4752 942 883 591
PfoI T'CCnGG_A
Cuts at: 2094 2094
Size: 7168
PmeI GTTT'AAAC
Cuts at: 4472 4472
Size: 7168
PmlI CAC'GTG
Cuts at: 2792 3229 2792
Size: 437 6731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3805 3837 5237 5269 6750 6782 3805
Size: 32 1400 32 1481 32 4191
Fragments arranged by size:
4191 1481 1400 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2875 3153 2875
Size: 278 6890
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7168
PsiI TTA'TAA
Cuts at: 4304 4738 4304
Size: 434 6734
PspOMI G'GGCC_C
Cuts at: 2859 2859
Size: 7168
PspXI vC'TCGA_Gb
Cuts at: 2652 2652
Size: 7168
PstI C_TGCA'G
Cuts at: 830 4520 830
Size: 3690 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7168
PvuII CAG'CTG
Cuts at: 2969 3299 2969
Size: 330 6838
SacI G_AGCT'C
Cuts at: 2382 2949 4374 2382
Size: 567 1425 5176
Fragments arranged by size:
5176 1425 567
SalI G'TCGA_C
Cuts at: 1936 4510 1936
Size: 2574 4594
SbfI CC_TGCA'GG
Cuts at: 4520 4520
Size: 7168
ScaI AGT'ACT
Cuts at: 4163 5416 7128 4163
Size: 1253 1712 4203
Fragments arranged by size:
4203 1712 1253
SfcI C'TryA_G
Cuts at: 826 1045 2254 4516 5651 6332 6523 826
Size: 219 1209 2262 1135 681 191 1471
Fragments arranged by size:
2262 1471 1209 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 7168
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7168
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7168
SmaI CCC'GGG
Cuts at: 1872 4495 1872
Size: 2623 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2652 2712 3221 3866 4141 5272
Size: 197 926 709 60 509 645 275 1131
Cuts at: 5272 6173 6435 820
Size: 901 262 1553
Fragments arranged by size:
1553 1131 926 901 709 645 509 275
262 197 60
SnaBI TAC'GTA
Cuts at: 493 4959 493
Size: 4466 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7168
SphI G_CATG'C
Cuts at: 1132 4526 1132
Size: 3394 3774
SspI AAT'ATT
Cuts at: 5 52 5092 5
Size: 47 5040 2081
Fragments arranged by size:
5040 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2863 2927 4607 513
Size: 552 939 321 538 64 1680 3074
Fragments arranged by size:
3074 1680 939 552 538 321 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2454 2463 4391 5195 5212 5365 5550
Size: 1255 874 9 1928 804 17 153 185
Cuts at: 5550 5995 325
Size: 445 1498
Fragments arranged by size:
1928 1498 1255 874 804 445 185 153
17 9
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3200 4161 5414
Size: 274 80 33 51 1443 1223 961 1253
Cuts at: 5414 7126 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1253 1223 961 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7128 7160 7128
Size: 32 7136
Tth111I GACn'n_nGTC
Cuts at: 1316 3835 1316
Size: 2519 4649
XbaI T'CTAG_A
Cuts at: 4000 4504 4000
Size: 504 6664
XhoI C'TCGA_G
Cuts at: 1943 2652 1943
Size: 709 6459
XmaI C'CCGG_G
Cuts at: 1870 4493 1870
Size: 2623 4545
XmnI GAAnn'nnTTC
Cuts at: 2090 2163 2291 2844 5297 2090
Size: 73 128 553 2453 3961
Fragments arranged by size:
3961 2453 553 128 73
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2764 3495 4976 276
Size: 53 83 186 936 1230 731 1481 2468
Fragments arranged by size:
2468 1481 1230 936 731 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII FseI FspAI NruI NsiI
PacI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI